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sarcomas  (Novus Biologicals)


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    Structured Review

    Novus Biologicals sarcomas
    Sarcomas, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microarrays/Human+Various+Tissue+MicroArray+(Sarcoma)/pmc08319167-181-9-16
    Average 91 stars, based on 3 article reviews
    sarcomas - by Bioz Stars, 2026-10
    91/100 stars

    Images

    Related Articles

    Immunohistochemistry:

    Article Title: PIK3R3, part of the regulatory domain of PI3K, is upregulated in sarcoma stem-like cells and promotes invasion, migration, and chemotherapy resistance
    Article Snippet: Commercially available paraffin-embedded tissue array slides containing 79 human sarcomas and 4 human normal tissues (NBP2-30332, Novus Biologicals, and T242, US Biomax, Inc., USA) were purchased.. Sections were deparaffinized, then incubated with anti-human CD133, PIK3R3 (SR44-06; Novus Biologicals), and Nanog (ab80892; Abcam) in a solution of PBS with 1% BSA and 0.1% Triton X-100 at 4 °C overnight.Sections were deparaffinized, then incubated with anti-human CD133, PIK3R3 (SR44-06; Novus Biologicals), and Nanog (ab80892; Abcam) in a solution of PBS with 1% BSA and 0.1% Triton X-100 at 4 °C overnight.

    Article Title: PIK3R3, part of the regulatory domain of PI3K, is upregulated in sarcoma stem-like cells and promotes invasion, migration, and chemotherapy resistance
    Article Snippet: Protein expression of PIK3R3 and the stem cell transcription factor Nanog were greater in CD133+ compared with CD133– cells (Fig. ), while levels of other stem cell transcription factors including Sox2, Oct-4, and c-Myc were not consistently increased.Protein expression of PIK3R3 and the stem cell transcription factor Nanog were greater in CD133+ compared with CD133– cells (Fig. ), while levels of other stem cell transcription factors including Sox2, Oct-4, and c-Myc were not consistently increased.. Finally, to examine whether PIK3R3 is generally overexpressed in human sarcomas, we immunostained commercially available tissue microarrays containing 79 human sarcomas (including diverse subtypes) and 4 human normal tissues (NBP2-30332, Novus Biologicals, and T242, US Biomax).. Positive cells of CD133, Nanog, or PIK3R3 were 5.2–12.7-fold higher in tumor tissue compared with normal tissue as determined by immunostaining (Fig. ).Positive cells of CD133, Nanog, or PIK3R3 were 5.2–12.7-fold higher in tumor tissue compared with normal tissue as determined by immunostaining (Fig. ).

    Article Title: PIK3R3, part of the regulatory domain of PI3K, is upregulated in sarcoma stem-like cells and promotes invasion, migration, and chemotherapy resistance
    Article Snippet: examine whether PIK3R3 is generally overexpressed in human sarcomas, we immunostained commercially available tissue microarrays containing 79 human sarcomas (including diverse subtypes) and 4 human normal tissues (NBP2-30332, Novus Biologicals, and T242, US Biomax). Positive cells of CD133, Nanog, or PIK3R3 were 5.2–12.7-fold higher in tumor tissue compared with normal tissue as determined by immunostaining (Fig. 1F ). Together, these results show

    Article Title: Enrichment of the embryonic stem cell reprogramming factors Oct4, Nanog, Myc, and Sox2 in benign and malignant vascular tumors
    Article Snippet: Blood vessel disease spectrum tissue arrays containing various vascular tumors and non-diseased controls were purchased from US Biomax, Inc. (#SO8010).Blood vessel disease spectrum tissue arrays containing various vascular tumors and non-diseased controls were purchased from US Biomax, Inc. (#SO8010).. The sarcoma tissue arrays were purchased from Novus Biologicals (#NBP2 = 30332).. For detection of protein expression, tissue arrays were labeled with anti-Myc (Cat# ab32072; Abcam), anti-Oct4 (Cat# ab18976; Abcam), anti-Sox2 (Cat# ab97959; Abcam), anti-Klf4 (Cat# ab118961; Abcam), and anti-Nanog (Cat# ab80892; Abcam) antibodies.For detection of protein expression, tissue arrays were labeled with anti-Myc (Cat# ab32072; Abcam), anti-Oct4 (Cat# ab18976; Abcam), anti-Sox2 (Cat# ab97959; Abcam), anti-Klf4 (Cat# ab118961; Abcam), and anti-Nanog (Cat# ab80892; Abcam) antibodies.

    Immunofluorescence:

    Article Title: PIK3R3, part of the regulatory domain of PI3K, is upregulated in sarcoma stem-like cells and promotes invasion, migration, and chemotherapy resistance
    Article Snippet: Commercially available paraffin-embedded tissue array slides containing 79 human sarcomas and 4 human normal tissues (NBP2-30332, Novus Biologicals, and T242, US Biomax, Inc., USA) were purchased.. Sections were deparaffinized, then incubated with anti-human CD133, PIK3R3 (SR44-06; Novus Biologicals), and Nanog (ab80892; Abcam) in a solution of PBS with 1% BSA and 0.1% Triton X-100 at 4 °C overnight.Sections were deparaffinized, then incubated with anti-human CD133, PIK3R3 (SR44-06; Novus Biologicals), and Nanog (ab80892; Abcam) in a solution of PBS with 1% BSA and 0.1% Triton X-100 at 4 °C overnight.

    Article Title: PIK3R3, part of the regulatory domain of PI3K, is upregulated in sarcoma stem-like cells and promotes invasion, migration, and chemotherapy resistance
    Article Snippet: Protein expression of PIK3R3 and the stem cell transcription factor Nanog were greater in CD133+ compared with CD133– cells (Fig. ), while levels of other stem cell transcription factors including Sox2, Oct-4, and c-Myc were not consistently increased.Protein expression of PIK3R3 and the stem cell transcription factor Nanog were greater in CD133+ compared with CD133– cells (Fig. ), while levels of other stem cell transcription factors including Sox2, Oct-4, and c-Myc were not consistently increased.. Finally, to examine whether PIK3R3 is generally overexpressed in human sarcomas, we immunostained commercially available tissue microarrays containing 79 human sarcomas (including diverse subtypes) and 4 human normal tissues (NBP2-30332, Novus Biologicals, and T242, US Biomax).. Positive cells of CD133, Nanog, or PIK3R3 were 5.2–12.7-fold higher in tumor tissue compared with normal tissue as determined by immunostaining (Fig. ).Positive cells of CD133, Nanog, or PIK3R3 were 5.2–12.7-fold higher in tumor tissue compared with normal tissue as determined by immunostaining (Fig. ).

    Article Title: PIK3R3, part of the regulatory domain of PI3K, is upregulated in sarcoma stem-like cells and promotes invasion, migration, and chemotherapy resistance
    Article Snippet: examine whether PIK3R3 is generally overexpressed in human sarcomas, we immunostained commercially available tissue microarrays containing 79 human sarcomas (including diverse subtypes) and 4 human normal tissues (NBP2-30332, Novus Biologicals, and T242, US Biomax). Positive cells of CD133, Nanog, or PIK3R3 were 5.2–12.7-fold higher in tumor tissue compared with normal tissue as determined by immunostaining (Fig. 1F ). Together, these results show

    Article Title: Enrichment of the embryonic stem cell reprogramming factors Oct4, Nanog, Myc, and Sox2 in benign and malignant vascular tumors
    Article Snippet: Blood vessel disease spectrum tissue arrays containing various vascular tumors and non-diseased controls were purchased from US Biomax, Inc. (#SO8010).Blood vessel disease spectrum tissue arrays containing various vascular tumors and non-diseased controls were purchased from US Biomax, Inc. (#SO8010).. The sarcoma tissue arrays were purchased from Novus Biologicals (#NBP2 = 30332).. For detection of protein expression, tissue arrays were labeled with anti-Myc (Cat# ab32072; Abcam), anti-Oct4 (Cat# ab18976; Abcam), anti-Sox2 (Cat# ab97959; Abcam), anti-Klf4 (Cat# ab118961; Abcam), and anti-Nanog (Cat# ab80892; Abcam) antibodies.For detection of protein expression, tissue arrays were labeled with anti-Myc (Cat# ab32072; Abcam), anti-Oct4 (Cat# ab18976; Abcam), anti-Sox2 (Cat# ab97959; Abcam), anti-Klf4 (Cat# ab118961; Abcam), and anti-Nanog (Cat# ab80892; Abcam) antibodies.



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    SCIENION non contact inkjet microarray printer
    The VANC-D4 assay (A) Fabrication of the VANC-D4 assay. SI-ATRP is used to grow POEGMA-co-GMA brushes on glass slides. Following SI-ATRP, VANC-BSA is <t>inkjet</t> printed into the polymer brush to create stable spots and fluorescently labeled anti-VANC antibodies (termed dAb) are printed on trehalose pads to create labile spots. (B) Sequential steps of the VANC-D4 assay. The steps of the VANC-D4 are (i) dispensing a blood sample on the surface, (ii) dissolution of the trehalose pads to release the fluorescently labeled dAb into solution, (iii) diffusion of the labeled dAb in the sample volume, (iv) detection of fluorescence signal from the antibody bound to immobilized VANC-BSA. (C) Representative VANC-D4 assay response in the presence of (i) no drug, (ii) low drug, and (iii) high drug concentration. (D) VANC monitoring across its clinical range using VANC-spiked whole blood diluted 100-fold in running buffer, analyzed in triplicate. The clinical range for the test is 1–45 μg/mL. Intensity values are shown, with error bars that represent the standard error of the means for n = 3 separate runs. The LOD for the presented dose-response curve was calculated to be 0.8 μg/mL.
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    Image Search Results


    The VANC-D4 assay (A) Fabrication of the VANC-D4 assay. SI-ATRP is used to grow POEGMA-co-GMA brushes on glass slides. Following SI-ATRP, VANC-BSA is inkjet printed into the polymer brush to create stable spots and fluorescently labeled anti-VANC antibodies (termed dAb) are printed on trehalose pads to create labile spots. (B) Sequential steps of the VANC-D4 assay. The steps of the VANC-D4 are (i) dispensing a blood sample on the surface, (ii) dissolution of the trehalose pads to release the fluorescently labeled dAb into solution, (iii) diffusion of the labeled dAb in the sample volume, (iv) detection of fluorescence signal from the antibody bound to immobilized VANC-BSA. (C) Representative VANC-D4 assay response in the presence of (i) no drug, (ii) low drug, and (iii) high drug concentration. (D) VANC monitoring across its clinical range using VANC-spiked whole blood diluted 100-fold in running buffer, analyzed in triplicate. The clinical range for the test is 1–45 μg/mL. Intensity values are shown, with error bars that represent the standard error of the means for n = 3 separate runs. The LOD for the presented dose-response curve was calculated to be 0.8 μg/mL.

    Journal: iScience

    Article Title: A rapid point-of-care test for vancomycin monitoring at the clinical bedside

    doi: 10.1016/j.isci.2026.116064

    Figure Lengend Snippet: The VANC-D4 assay (A) Fabrication of the VANC-D4 assay. SI-ATRP is used to grow POEGMA-co-GMA brushes on glass slides. Following SI-ATRP, VANC-BSA is inkjet printed into the polymer brush to create stable spots and fluorescently labeled anti-VANC antibodies (termed dAb) are printed on trehalose pads to create labile spots. (B) Sequential steps of the VANC-D4 assay. The steps of the VANC-D4 are (i) dispensing a blood sample on the surface, (ii) dissolution of the trehalose pads to release the fluorescently labeled dAb into solution, (iii) diffusion of the labeled dAb in the sample volume, (iv) detection of fluorescence signal from the antibody bound to immobilized VANC-BSA. (C) Representative VANC-D4 assay response in the presence of (i) no drug, (ii) low drug, and (iii) high drug concentration. (D) VANC monitoring across its clinical range using VANC-spiked whole blood diluted 100-fold in running buffer, analyzed in triplicate. The clinical range for the test is 1–45 μg/mL. Intensity values are shown, with error bars that represent the standard error of the means for n = 3 separate runs. The LOD for the presented dose-response curve was calculated to be 0.8 μg/mL.

    Article Snippet: Following polymerization, D4 assays were fabricated using non-contact inkjet printing methods as described elsewhere., VANC-BSA was printed using a non-contact inkjet microarray printer (Scienion, sciFLEXARRAYER Type S12).

    Techniques: Polymer, Labeling, Dissolution, Diffusion-based Assay, Fluorescence, Concentration Assay