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Digilab Inc microarray robot omnigrid accent
Microarray Robot Omnigrid Accent, supplied by Digilab Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microarraying+robot+omnigrid+accent/microarraying+robot+microgrid+610/pmc04589137-29-21-25
Average 90 stars, based on 1 article reviews
microarray robot omnigrid accent - by Bioz Stars, 2026-11
90/100 stars

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Microarray:

Article Title: The role of indoleamine 2,3-dioxygenase-aryl hydrocarbon receptor pathway in the TLR4-induced tolerogenic phenotype in human DCs.
Article Snippet: .. After denaturation, samples were spotted onto nitrocellulose-coated glass slides (Grace Bio-labs) with a microarray robot (MicroGrid 610, Digilab). .. Then, slides were incubated overnight in blocking solution (0.2% I-Block (Thermo Fisher Scientific), Tween-20 (0.1%) in PBS (Sigma-Aldrich) at 4 °C.

Article Title: Clinical utility of reverse phase protein array for molecular classification of breast cancer.
Article Snippet: Reverse Phase Protein Array (RPPA) represents a sensitive and high-throughput technique allowing simultaneous quantitation of protein expression levels in biological samples.. This study aimed to confirm the ability of RPPA to classify archival formalin-fixed paraffin-embedded (FFPE) breast cancer tissues into molecular classes used in the Nottingham prognostic index plus (NPI?) determined by immunohistochemistry (IHC).. Proteins were extracted from FFPE breast cancer tissues using three extraction protocols: the Q-proteome FFPE Tissue Kit (Qiagen, Hilden, Germany) and two in-house methods using Laemmli buffer with either incubation for 20 min or 2 h at 105 C. Two preparation methods, full-face sections and macrodissection, were used to assess the yield and quality of protein extracts.

Article Title: The mannose receptor negatively modulates the Toll-like receptor 4-aryl hydrocarbon receptor-indoleamine 2,3-dioxygenase axis in dendritic cells affecting T helper cell polarization.
Article Snippet: .. After denaturation, samples were spotted onto nitrocellulose-coated glass slides (Grace Bio-Labs, Bend, Ore) with a microarray robot (MicroGrid 610; Digilab, Marlborough, Mass). .. Then slides were incubated overnight in blocking solution (0.2% IBlock [Thermo Fisher Scientific] and 0.1% Tween-20 in PBS [SigmaAldrich]) at 48C.

Article Title: A Pro-Inflammatory Signature Constitutively Activated in Monogenic Autoinflammatory Diseases
Article Snippet: .. Briefly, samples were spotted in duplicates onto a nitrocellulose-coated glass slide (Grace Bio-labs, Bend, OR, USA) using a microarray robot (MicroGrid 610, Digilab, Marlborough, MA, USA). .. The printed slides were blocked and incubated with specific primary antibodies listed in (Cell Signalling) overnight at 4 °C, with shaking.

Article Title: Charge Characteristics of Agouti-Related Protein Implicate Potent Involvement of Heparan Sulfate Proteoglycans in Metabolic Function
Article Snippet: .. Amine(-NH 2 )-linked heparan sulfate glycan compounds (Glycan Therapeutics) were immobilized on NHS-activated surface-coated slides (Nexterion Slide-H, Applied Microarrays) using a robotic microarray printer (Microgrid II, Digilab) that was equipped with StealthSMP4B microarray pins (Telechem) to couple heparan sulfate glycan compounds by covalent binding via (-NH 2 ) reactive chemistry. ..

Article Title: The role of indoleamine 2,3-dioxygenase-aryl hydrocarbon receptor pathway in the TLR4-induced tolerogenic phenotype in human DCs
Article Snippet: .. After denaturation, samples were spotted onto nitrocellulose-coated glass slides (Grace Bio-labs) with a microarray robot (MicroGrid 610, Digilab). .. Then, slides were incubated overnight in blocking solution (0.2% I-Block (Thermo Fisher Scientific), Tween-20 (0.1%) in PBS (Sigma-Aldrich) at 4 °C.

other:

Article Title: New and Improved Techniques for the Study of Pathogenic Fungi.
Article Snippet: Fungal pathogens pose serious threats to human, plant, and ecosystem health.. Improved diagnostics and antifungal strategies are therefore urgently required.. Here, we review recent developments in online bioinformatic tools and associated interactive data archives, which enable sophisticated comparative genomics and functional analysis of fungal pathogens in silico.

Article Title: SMAD4 loss enables EGF, TGFβ1 and S100A8/A9 induced activation of critical pathways to invasion in human pancreatic adenocarcinoma cells
Article Snippet: Briefly, cell lysates obtained from 800,000 cells (ø 10 cm Petri dishes) were solubilized in 4×SDS loading buffer (Sigma-Aldrich, Milano, Italy) and heated for 5 minutes at 95°C.

Glycoproteomics:

Article Title: Charge Characteristics of Agouti-Related Protein Implicate Potent Involvement of Heparan Sulfate Proteoglycans in Metabolic Function
Article Snippet: .. Amine(-NH 2 )-linked heparan sulfate glycan compounds (Glycan Therapeutics) were immobilized on NHS-activated surface-coated slides (Nexterion Slide-H, Applied Microarrays) using a robotic microarray printer (Microgrid II, Digilab) that was equipped with StealthSMP4B microarray pins (Telechem) to couple heparan sulfate glycan compounds by covalent binding via (-NH 2 ) reactive chemistry. ..

Binding Assay:

Article Title: Charge Characteristics of Agouti-Related Protein Implicate Potent Involvement of Heparan Sulfate Proteoglycans in Metabolic Function
Article Snippet: .. Amine(-NH 2 )-linked heparan sulfate glycan compounds (Glycan Therapeutics) were immobilized on NHS-activated surface-coated slides (Nexterion Slide-H, Applied Microarrays) using a robotic microarray printer (Microgrid II, Digilab) that was equipped with StealthSMP4B microarray pins (Telechem) to couple heparan sulfate glycan compounds by covalent binding via (-NH 2 ) reactive chemistry. ..



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Digilabs Inc omnigrid accent robotic microarray
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Preparation and Characterization of Functionalized Microarray Glass Slides: A) Intensity profile analysis of fluorescence spots on functionalized glass slides prepared by conventional reaction or microwave reaction. B) Fluorescent dye conjugation using the PEG-NHS slide (5-AMF and Alexafluor555) or PEG-amine slide (ZW800-1 NHS ester) functionalized by the microwave reaction. Scale bars = 200 μm. C) Image of ligand-patterned slides after robotic <t>microarraying</t> of functionalized slides.
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Preparation and Characterization of Functionalized Microarray Glass Slides: A) Intensity profile analysis of fluorescence spots on functionalized glass slides prepared by conventional reaction or microwave reaction. B) Fluorescent dye conjugation using the PEG-NHS slide (5-AMF and Alexafluor555) or PEG-amine slide (ZW800-1 NHS ester) functionalized by the microwave reaction. Scale bars = 200 μm. C) Image of ligand-patterned slides after robotic <t>microarraying</t> of functionalized slides.
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Preparation and Characterization of Functionalized Microarray Glass Slides: A) Intensity profile analysis of fluorescence spots on functionalized glass slides prepared by conventional reaction or microwave reaction. B) Fluorescent dye conjugation using the PEG-NHS slide (5-AMF and Alexafluor555) or PEG-amine slide (ZW800-1 NHS ester) functionalized by the microwave reaction. Scale bars = 200 μm. C) Image of ligand-patterned slides after robotic <t>microarraying</t> of functionalized slides.
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Preparation and Characterization of Functionalized Microarray Glass Slides: A) Intensity profile analysis of fluorescence spots on functionalized glass slides prepared by conventional reaction or microwave reaction. B) Fluorescent dye conjugation using the PEG-NHS slide (5-AMF and Alexafluor555) or PEG-amine slide (ZW800-1 NHS ester) functionalized by the microwave reaction. Scale bars = 200 μm. C) Image of ligand-patterned slides after robotic microarraying of functionalized slides.

Journal: Advanced functional materials

Article Title: Rapid and Facile Microwave-Assisted Surface Chemistry for Functionalized Microarray Slides

doi: 10.1002/adfm.201102033

Figure Lengend Snippet: Preparation and Characterization of Functionalized Microarray Glass Slides: A) Intensity profile analysis of fluorescence spots on functionalized glass slides prepared by conventional reaction or microwave reaction. B) Fluorescent dye conjugation using the PEG-NHS slide (5-AMF and Alexafluor555) or PEG-amine slide (ZW800-1 NHS ester) functionalized by the microwave reaction. Scale bars = 200 μm. C) Image of ligand-patterned slides after robotic microarraying of functionalized slides.

Article Snippet: Microarray Printing of Ligand Arrays A contact type microarraying robot (OmniGrid Accent, DigiLab, Inc., Holliston, MA) mounted with SMP 11 pins (ArryIt, Telechem, Sunnyvale, CA) was used to print the ligands onto the functionalized surface.

Techniques: Microarray, Fluorescence, Conjugation Assay

Preparation and Characterization of Functionalized Microarray Glass Slides: A) Intensity profile analysis of fluorescence spots on functionalized glass slides prepared by conventional reaction or microwave reaction. B) Fluorescent dye conjugation using the PEG-NHS slide (5-AMF and Alexafluor555) or PEG-amine slide (ZW800-1 NHS ester) functionalized by the microwave reaction. Scale bars = 200 μm. C) Image of ligand-patterned slides after robotic microarraying of functionalized slides.

Journal: Advanced functional materials

Article Title: Rapid and Facile Microwave-Assisted Surface Chemistry for Functionalized Microarray Slides

doi: 10.1002/adfm.201102033

Figure Lengend Snippet: Preparation and Characterization of Functionalized Microarray Glass Slides: A) Intensity profile analysis of fluorescence spots on functionalized glass slides prepared by conventional reaction or microwave reaction. B) Fluorescent dye conjugation using the PEG-NHS slide (5-AMF and Alexafluor555) or PEG-amine slide (ZW800-1 NHS ester) functionalized by the microwave reaction. Scale bars = 200 μm. C) Image of ligand-patterned slides after robotic microarraying of functionalized slides.

Article Snippet: A contact type microarraying robot (OmniGrid Accent, DigiLab, Inc., Holliston, MA) mounted with SMP 11 pins (ArryIt, Telechem, Sunnyvale, CA) was used to print the ligands onto the functionalized surface.

Techniques: Microarray, Fluorescence, Conjugation Assay