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BioMicro Systems Inc maui mixer hybridization chamber
Maui Mixer Hybridization Chamber, supplied by BioMicro Systems Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microarray+user+interface+hybridization+chamber/maui+mixer+fl+hybridization+chambers/pmc05228284-66-23-28
Average 90 stars, based on 1 article reviews
maui mixer hybridization chamber - by Bioz Stars, 2026-10
90/100 stars

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Hybridization:

Article Title: Molecular dissection of the migrating posterior lateral line primordium during early development in zebrafish
Article Snippet: .. Hybridizations were performed overnight at 45°C in Maui Mixer FL hybridization chambers (BioMicro Systems). ..

Article Title: Hydrogenase isolated from
Article Snippet: 30 μl of the concentrated Cy5-labeled cDNAs were suspended in a hybridization solution (GenoCheck, Korea). .. The labeled cDNAs were located on the microarray, and then covered by a MAUI Mixer X4 hybridization chamber (BioMicro Systems, Salt Lake City, Utah). .. The slides were hybridized using MAUI 12-bay systems (BioMicro Systems, Salt Lake City, Utah) at 42° C. for 12 hours.

Article Title: A gene-expression screen identifies a non-toxic sumoylation inhibitor that mimics SUMO-less human LRH-1 in liver
Article Snippet: Total RNA was purified using RNAeasy kit (Life technologies) according to the manufacturer’s protocol. .. Hybridizations were performed at 65 ̊C for 16 hr using mixers compatible with the MAUI hybridization systems (BioMicro Systems, Salt Lake City, UT). .. Arrays were scanned using an Axon Scanner 4000B, and data analyzed by GenePix 6.0 software (Molecular Devices).

Article Title: Impact of chromosome 17q deletion in the primary lesion of colorectal cancer on liver metastasis
Article Snippet: The labeled test and reference DNA were then ethanol precipitated in the presence of Cot-1 DNA (Thermo Fisher Scientific, Inc.), redissolved in a hybridization mix and subsequently denatured at 70°C for 10 min. .. Following incubation at 42°C for 5 min, the mixture was applied to a Whole Genome DNA Microarray (LinkGenomics, Tokyo, Japan) covered with an MAUI ® Mixer Hybridization Chamber (BioMicro Systems, Inc., Salt Lake City, USA). ..

Article Title: Accumulation of cholesterol and increased demand for zinc in serum-deprived RPE cells
Article Snippet: .. Hybridization of the labeled samples to the arrays was performed overnight in a MAUI mixing incubator (Biomicro, Salt lake City, UT). .. Next, the arrays were washed, dried, and scanned in a ScanArray Express (Perkin Elmer, Waltham, MA) measuring green (A565) and red (A670) fluorescence.

Article Title: Mutated MITF-E87R in Melanoma Enhances Tumor Progression via S100A4.
Article Snippet: The generated aminoallyl UTP labeled RNAs were then coupled with either Cy3 or Cy5 mono NHS ester CyDyes (GE Healthcare, Piscataway, NJ). .. After purification, the labeled antisense RNAs was hybridized overnight to the oligonucleotide chips in 5X SSC, 25% formamide and 0.2% M AN US CR IP T AC CE PT ED 14 SDS buffer at 45 °C using Maui Mixer FL hybridization chambers (BioMicro Systems, Salt Lake City, UT). ..

Article Title: Antitumor effects of novel mAbs against cationic amino acid transporter 1 (CAT1) on human CRC with amplified CAT1 gene
Article Snippet: .. After incubation at 42°C for 5 minutes, the mixture was applied to the whole‐genome DNA microarray (LinkGenomics) covered with an MAUI Mixer hybridization chamber (BioMicro Systems). ..

Article Title: Development of a cDNA microarray for the measurement of gene expression in the sheep scab mite Psoroptes ovis
Article Snippet: The aRNA was indirectly coupled with a fluorescent Cy dye, either Cy3 or Cy5 (GE Bioscience, UK) by the incorporation of a 5-(3-aminoallyl)-UTP in a cDNA synthesis reaction, followed by dye binding and purification following the protocol available on the ARK Genomics website ( http://www.ark-genomics.org/protocols/DyeCouplingAaRNA.php ). .. Hybridisations were performed on a MAUI microarray hybridisation system (BioMicro Systems, USA) using the MAUI Mixer A4 hybridisation cassette with a 16 μl fill volume per chamber (BioMicro Systems, USA) which allowed the four microarrays on a single slide to be treated independently. .. The hybridisation mixture consisted of the following: 13.6 μl of a 1:1 mixture of UltraHyb (Ambion, UK) and 2 × SSC (Sigma, UK); 1.6 μl PolyA (10 mg/ml, Sigma, UK); 1.6 μl of 25 pmol Cy3 labelled sample; 1.6 μl of 25 pmol Cy5 labelled samples; 0.8 μl Salmon Sperm DNA (10 mg/ml, Invitrogen, UK) and 0.8 μl BSA (50 mg/ml, Ambion, UK) to a final volume of 20 μl.

Labeling:

Article Title: Hydrogenase isolated from
Article Snippet: 30 μl of the concentrated Cy5-labeled cDNAs were suspended in a hybridization solution (GenoCheck, Korea). .. The labeled cDNAs were located on the microarray, and then covered by a MAUI Mixer X4 hybridization chamber (BioMicro Systems, Salt Lake City, Utah). .. The slides were hybridized using MAUI 12-bay systems (BioMicro Systems, Salt Lake City, Utah) at 42° C. for 12 hours.

Article Title: Accumulation of cholesterol and increased demand for zinc in serum-deprived RPE cells
Article Snippet: .. Hybridization of the labeled samples to the arrays was performed overnight in a MAUI mixing incubator (Biomicro, Salt lake City, UT). .. Next, the arrays were washed, dried, and scanned in a ScanArray Express (Perkin Elmer, Waltham, MA) measuring green (A565) and red (A670) fluorescence.

Article Title: Mutated MITF-E87R in Melanoma Enhances Tumor Progression via S100A4.
Article Snippet: The generated aminoallyl UTP labeled RNAs were then coupled with either Cy3 or Cy5 mono NHS ester CyDyes (GE Healthcare, Piscataway, NJ). .. After purification, the labeled antisense RNAs was hybridized overnight to the oligonucleotide chips in 5X SSC, 25% formamide and 0.2% M AN US CR IP T AC CE PT ED 14 SDS buffer at 45 °C using Maui Mixer FL hybridization chambers (BioMicro Systems, Salt Lake City, UT). ..

Microarray:

Article Title: Hydrogenase isolated from
Article Snippet: 30 μl of the concentrated Cy5-labeled cDNAs were suspended in a hybridization solution (GenoCheck, Korea). .. The labeled cDNAs were located on the microarray, and then covered by a MAUI Mixer X4 hybridization chamber (BioMicro Systems, Salt Lake City, Utah). .. The slides were hybridized using MAUI 12-bay systems (BioMicro Systems, Salt Lake City, Utah) at 42° C. for 12 hours.

Article Title: Impact of chromosome 17q deletion in the primary lesion of colorectal cancer on liver metastasis
Article Snippet: The labeled test and reference DNA were then ethanol precipitated in the presence of Cot-1 DNA (Thermo Fisher Scientific, Inc.), redissolved in a hybridization mix and subsequently denatured at 70°C for 10 min. .. Following incubation at 42°C for 5 min, the mixture was applied to a Whole Genome DNA Microarray (LinkGenomics, Tokyo, Japan) covered with an MAUI ® Mixer Hybridization Chamber (BioMicro Systems, Inc., Salt Lake City, USA). ..

Article Title: Antitumor effects of novel mAbs against cationic amino acid transporter 1 (CAT1) on human CRC with amplified CAT1 gene
Article Snippet: .. After incubation at 42°C for 5 minutes, the mixture was applied to the whole‐genome DNA microarray (LinkGenomics) covered with an MAUI Mixer hybridization chamber (BioMicro Systems). ..

Article Title: Development of a cDNA microarray for the measurement of gene expression in the sheep scab mite Psoroptes ovis
Article Snippet: The aRNA was indirectly coupled with a fluorescent Cy dye, either Cy3 or Cy5 (GE Bioscience, UK) by the incorporation of a 5-(3-aminoallyl)-UTP in a cDNA synthesis reaction, followed by dye binding and purification following the protocol available on the ARK Genomics website ( http://www.ark-genomics.org/protocols/DyeCouplingAaRNA.php ). .. Hybridisations were performed on a MAUI microarray hybridisation system (BioMicro Systems, USA) using the MAUI Mixer A4 hybridisation cassette with a 16 μl fill volume per chamber (BioMicro Systems, USA) which allowed the four microarrays on a single slide to be treated independently. .. The hybridisation mixture consisted of the following: 13.6 μl of a 1:1 mixture of UltraHyb (Ambion, UK) and 2 × SSC (Sigma, UK); 1.6 μl PolyA (10 mg/ml, Sigma, UK); 1.6 μl of 25 pmol Cy3 labelled sample; 1.6 μl of 25 pmol Cy5 labelled samples; 0.8 μl Salmon Sperm DNA (10 mg/ml, Invitrogen, UK) and 0.8 μl BSA (50 mg/ml, Ambion, UK) to a final volume of 20 μl.

Incubation:

Article Title: Impact of chromosome 17q deletion in the primary lesion of colorectal cancer on liver metastasis
Article Snippet: The labeled test and reference DNA were then ethanol precipitated in the presence of Cot-1 DNA (Thermo Fisher Scientific, Inc.), redissolved in a hybridization mix and subsequently denatured at 70°C for 10 min. .. Following incubation at 42°C for 5 min, the mixture was applied to a Whole Genome DNA Microarray (LinkGenomics, Tokyo, Japan) covered with an MAUI ® Mixer Hybridization Chamber (BioMicro Systems, Inc., Salt Lake City, USA). ..

Article Title: Antitumor effects of novel mAbs against cationic amino acid transporter 1 (CAT1) on human CRC with amplified CAT1 gene
Article Snippet: .. After incubation at 42°C for 5 minutes, the mixture was applied to the whole‐genome DNA microarray (LinkGenomics) covered with an MAUI Mixer hybridization chamber (BioMicro Systems). ..

Purification:

Article Title: Mutated MITF-E87R in Melanoma Enhances Tumor Progression via S100A4.
Article Snippet: The generated aminoallyl UTP labeled RNAs were then coupled with either Cy3 or Cy5 mono NHS ester CyDyes (GE Healthcare, Piscataway, NJ). .. After purification, the labeled antisense RNAs was hybridized overnight to the oligonucleotide chips in 5X SSC, 25% formamide and 0.2% M AN US CR IP T AC CE PT ED 14 SDS buffer at 45 °C using Maui Mixer FL hybridization chambers (BioMicro Systems, Salt Lake City, UT). ..



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BioMicro Systems Inc microarray user interface hybridization chamber
qRT-PCR primers used to validate the <t> microarray </t> results
Microarray User Interface Hybridization Chamber, supplied by BioMicro Systems Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microarray+user+interface+hybridization+chamber/micro+array+user+interface+hybridization+system/pmc04811642-164-23-29
Average 90 stars, based on 1 article reviews
microarray user interface hybridization chamber - by Bioz Stars, 2026-10
90/100 stars
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qRT-PCR primers used to validate the  microarray  results

Journal: Microbiology

Article Title: Global transcriptional analysis of the stringent response in Enterococcus faecalis

doi: 10.1099/mic.0.060236-0

Figure Lengend Snippet: qRT-PCR primers used to validate the microarray results

Article Snippet: The slides were hybridized to a mixture containing equal amounts of test and reference cDNA for 16 h at 42 °C in a MicroArray User Interface (MAUI) hybridization chamber (BioMicro Systems).

Techniques: Microarray

qRT-PCR validation of  microarray  data

Journal: Microbiology

Article Title: Global transcriptional analysis of the stringent response in Enterococcus faecalis

doi: 10.1099/mic.0.060236-0

Figure Lengend Snippet: qRT-PCR validation of microarray data

Article Snippet: The slides were hybridized to a mixture containing equal amounts of test and reference cDNA for 16 h at 42 °C in a MicroArray User Interface (MAUI) hybridization chamber (BioMicro Systems).

Techniques: Biomarker Discovery, Microarray