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BioMicro Systems Inc maui sc hybridization chambers
Maui Sc Hybridization Chambers, supplied by BioMicro Systems Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microarray+user+interface+hybridization+chamber/maui+m4+hybridization+chambers/pmc03186354-121-20-24
Average 90 stars, based on 1 article reviews
maui sc hybridization chambers - by Bioz Stars, 2026-11
90/100 stars

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Construct:

Article Title: Virulence Gene- and Pandemic Group-Specific Marker Profiling of Clinical Vibrio parahaemolyticus Isolates
Article Snippet: .. Once constructed, the spotted microarrays were blocked with a 3% bovine serum albumin-casein solution for 15 min at room temperature, and the slides were outfitted with MAUI M4 hybridization chambers (BioMicro Systems, Salt Lake City, UT). .. Hybridization reactions (6.0 μl multiplex PCR amplicons, 2.4 μl 20× SSC [1× SSC is 0.15 M NaCl plus 0.015 M sodium citrate], 2.4 μl formamide, 0.8 μl 3% bovine serum albumin-casein solution, 0.2 μl hybridization-positive control, and 0.24 μl 10% sodium dodecyl sulfate) were denatured for 5 min at 95°C, applied to the microarray, and allowed to incubate for 2 h at 63°C with a MAUI hybridization system (BioMicro Systems).

Article Title: Virulence Gene- and Pandemic Group-Specific Marker Profiling of Clinical Vibrio parahaemolyticus Isolates
Article Snippet: .. Once constructed, the spotted microarrays were blocked with a 3% bovine serum albumin-casein solution for 15 min at room temperature, and the slides were outfitted with MAUI M4 hybridization chambers (BioMicro Systems, Salt Lake City, UT). .. Hybridization reactions (6.0 l multiplex PCR amplicons, 2.4 l 20 SSC [1 SSC is 0.15 M NaCl plus 0.015 M sodium citrate], 2.4 l formamide, 0.8 l 3% bovine serum albumin-casein solution, 0.2 l hybridization-positive control, and 0.24 l 10% sodium dodecyl sulfate) were denatured for 5 min at 95°C, applied to the microarray, and allowed to incubate for 2 h at 63°C with a MAUI hybridization system (BioMicro Systems).

Hybridization:

Article Title: Virulence Gene- and Pandemic Group-Specific Marker Profiling of Clinical Vibrio parahaemolyticus Isolates
Article Snippet: .. Once constructed, the spotted microarrays were blocked with a 3% bovine serum albumin-casein solution for 15 min at room temperature, and the slides were outfitted with MAUI M4 hybridization chambers (BioMicro Systems, Salt Lake City, UT). .. Hybridization reactions (6.0 μl multiplex PCR amplicons, 2.4 μl 20× SSC [1× SSC is 0.15 M NaCl plus 0.015 M sodium citrate], 2.4 μl formamide, 0.8 μl 3% bovine serum albumin-casein solution, 0.2 μl hybridization-positive control, and 0.24 μl 10% sodium dodecyl sulfate) were denatured for 5 min at 95°C, applied to the microarray, and allowed to incubate for 2 h at 63°C with a MAUI hybridization system (BioMicro Systems).

Article Title: Detection and differentiation of Clostridium botulinum type A strains using a focused DNA microarray.
Article Snippet: A focused oligonucleotide microarray featuring 62 probes targeting strain variable regions of the Clostridium botulinum strain ATCC 3502 genome sequence was developed to differentiate C. botulinum type A strains.. The strain variable regions were selected from deletions identified among a panel of 10 type A strains compared to the strain ATCC 3502 genome sequence using high density comparative genomic hybridization microarrays.. The focused microarray also featured specific probes for the detection of the neurotoxin genes of various serotypes (AeG), toxin gene cluster components (ha70 and orfX1), and fldB as a marker for proteolytic clostridia (Group I).

Article Title: Combining chemoselective ligation with polyhistidine-driven self-assembly for the modular display of biomolecules on quantum dots.
Article Snippet: .. Oligonucleotides coding for portions of the cholera toxin subunit (ctxA) and subunit (ctxB) genes were synthesized with a 5=-amino modifier separated from the oligo by a 12-carbon alkane spacer (Qiagen) and spotted onto aminosilane SuperChip glass slides (Nunc) in 100 mM sodium carbonate/bicarbonate buffer (pH 9.0) at concentrations of 0.08 50 M using an OmniGrid Accent Contact Printer (Genomic Solutions).25 Prior to hybridization, spotted microarrays were blocked with a 3% bovine serum albumin casein solution for 15 min at room temperature, and the slides were outfitted with MAUI M4 hybridization chambers (BioMicro Systems). .. QDDNA samples were prepared by incubating 550 nm QD-DHLA or QD-DHLA-PEG600 particles with 20 equiv of peptide-DNA conjugates (5 without TAMRA modification) as described above.

Article Title: Deregulation of microRNAs by HIV-1 Vpr Protein Leads to the Development of Neurocognitive Disorders
Article Snippet: 750 ng of total RNA was used for miRNA array analysis using miRCURY LNA TM microRNA Array, v. 11.0 (Exiqon, Woburn, MA). .. In brief, RNA was labeled using the miRCURY LNA TM miRNA, Hy5 Power Labeling Kit (Exiqon) per manufacturer's recommendations using Maui SC Hybridization Chambers (BioMicro Systems, Salt Lake City, UT). .. Array chips were scanned using Axon GenePix Scanner (Molecular Devices, Downingtown, PA) and Genepix 4000 image capture software (Molecular Devices, Downingtown, PA).

Article Title: HIV-1 Tat Protein Promotes Neuronal Dysfunction through Disruption of MicroRNAs
Article Snippet: 750 ng of total RNA was used for miRNA array analysis using miRCURY LNA TM microRNA Array, version 11.0 (Exiqon, Woburn, MA). .. In brief, RNA was labeled using the miRCURY LNA TM miRNA, Hy5 power labeling kit (Exiqon) as per manufacturer's recommendations using Maui SC hybridization chambers (BioMicro Systems, Salt Lake City, UT). .. Array chips were scanned using Axon GenePix Scanner (Molecular Devices, Downingtown, PA) and Genepix 4000 image capture software (Molecular Devices, Downingtown, PA).

Article Title: Combining Chemoselective Ligation with Polyhistidine-Driven Self-Assembly for the Modular Display of Biomolecules on Quantum Dots
Article Snippet: Microarray Hybridization Assays Oligonucleotides coding for portions of the cholera toxin α subunit ( ctxA ) and β subunit ( ctxB ) genes were synthesized with a 5′-amino modifier separated from the oligo by a 12-carbon alkane spacer (Qiagen) and spotted onto aminosilane SuperChip glass slides (Nunc) in 100 mM sodium carbonate/bicarbonate buffer (pH 9.0) at concentrations of 0.08–50 μM using an OmniGrid Accent Contact Printer (Genomic Solutions). .. 25 Prior to hybridization, spotted microarrays were blocked with a 3% bovine serum albumin-casein solution for 15 min at room temperature and the slides outfitted with MAUI ® M4 hybridization chambers (BioMicro ® Systems). ..

Article Title: Virulence Gene- and Pandemic Group-Specific Marker Profiling of Clinical Vibrio parahaemolyticus Isolates
Article Snippet: .. Once constructed, the spotted microarrays were blocked with a 3% bovine serum albumin-casein solution for 15 min at room temperature, and the slides were outfitted with MAUI M4 hybridization chambers (BioMicro Systems, Salt Lake City, UT). .. Hybridization reactions (6.0 l multiplex PCR amplicons, 2.4 l 20 SSC [1 SSC is 0.15 M NaCl plus 0.015 M sodium citrate], 2.4 l formamide, 0.8 l 3% bovine serum albumin-casein solution, 0.2 l hybridization-positive control, and 0.24 l 10% sodium dodecyl sulfate) were denatured for 5 min at 95°C, applied to the microarray, and allowed to incubate for 2 h at 63°C with a MAUI hybridization system (BioMicro Systems).

Synthesized:

Article Title: Combining chemoselective ligation with polyhistidine-driven self-assembly for the modular display of biomolecules on quantum dots.
Article Snippet: .. Oligonucleotides coding for portions of the cholera toxin subunit (ctxA) and subunit (ctxB) genes were synthesized with a 5=-amino modifier separated from the oligo by a 12-carbon alkane spacer (Qiagen) and spotted onto aminosilane SuperChip glass slides (Nunc) in 100 mM sodium carbonate/bicarbonate buffer (pH 9.0) at concentrations of 0.08 50 M using an OmniGrid Accent Contact Printer (Genomic Solutions).25 Prior to hybridization, spotted microarrays were blocked with a 3% bovine serum albumin casein solution for 15 min at room temperature, and the slides were outfitted with MAUI M4 hybridization chambers (BioMicro Systems). .. QDDNA samples were prepared by incubating 550 nm QD-DHLA or QD-DHLA-PEG600 particles with 20 equiv of peptide-DNA conjugates (5 without TAMRA modification) as described above.

Labeling:

Article Title: Deregulation of microRNAs by HIV-1 Vpr Protein Leads to the Development of Neurocognitive Disorders
Article Snippet: 750 ng of total RNA was used for miRNA array analysis using miRCURY LNA TM microRNA Array, v. 11.0 (Exiqon, Woburn, MA). .. In brief, RNA was labeled using the miRCURY LNA TM miRNA, Hy5 Power Labeling Kit (Exiqon) per manufacturer's recommendations using Maui SC Hybridization Chambers (BioMicro Systems, Salt Lake City, UT). .. Array chips were scanned using Axon GenePix Scanner (Molecular Devices, Downingtown, PA) and Genepix 4000 image capture software (Molecular Devices, Downingtown, PA).

Article Title: HIV-1 Tat Protein Promotes Neuronal Dysfunction through Disruption of MicroRNAs
Article Snippet: 750 ng of total RNA was used for miRNA array analysis using miRCURY LNA TM microRNA Array, version 11.0 (Exiqon, Woburn, MA). .. In brief, RNA was labeled using the miRCURY LNA TM miRNA, Hy5 power labeling kit (Exiqon) as per manufacturer's recommendations using Maui SC hybridization chambers (BioMicro Systems, Salt Lake City, UT). .. Array chips were scanned using Axon GenePix Scanner (Molecular Devices, Downingtown, PA) and Genepix 4000 image capture software (Molecular Devices, Downingtown, PA).



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BioMicro Systems Inc microarray user interface hybridization chamber
qRT-PCR primers used to validate the <t> microarray </t> results
Microarray User Interface Hybridization Chamber, supplied by BioMicro Systems Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microarray+user+interface+hybridization+chamber/micro+array+user+interface+hybridization+system/pmc04811642-164-23-29
Average 90 stars, based on 1 article reviews
microarray user interface hybridization chamber - by Bioz Stars, 2026-11
90/100 stars
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qRT-PCR primers used to validate the  microarray  results

Journal: Microbiology

Article Title: Global transcriptional analysis of the stringent response in Enterococcus faecalis

doi: 10.1099/mic.0.060236-0

Figure Lengend Snippet: qRT-PCR primers used to validate the microarray results

Article Snippet: The slides were hybridized to a mixture containing equal amounts of test and reference cDNA for 16 h at 42 °C in a MicroArray User Interface (MAUI) hybridization chamber (BioMicro Systems).

Techniques: Microarray

qRT-PCR validation of  microarray  data

Journal: Microbiology

Article Title: Global transcriptional analysis of the stringent response in Enterococcus faecalis

doi: 10.1099/mic.0.060236-0

Figure Lengend Snippet: qRT-PCR validation of microarray data

Article Snippet: The slides were hybridized to a mixture containing equal amounts of test and reference cDNA for 16 h at 42 °C in a MicroArray User Interface (MAUI) hybridization chamber (BioMicro Systems).

Techniques: Biomarker Discovery, Microarray