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phospho-specific antibody microarray slide  (Full Moon BioSystems)

 
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    Full Moon BioSystems phospho-specific antibody microarray slide
    Molecular mechanisms responsible for anti-AD effects of AAL extract. (a) HT22 cells were exposed to H2O2 in the absence or presence of AAL extract (50 μg/mL) for 6 h. Antibody <t>microarray</t> assay was performed using the phospho-specific antibody microarray slide (Full Moon BioSystems). For data acquisition, GenePix 4100A scanner (Axon Instrument, USA) was used. The normalization data were analyzed using Genowiz 4.0™ (Ocimum Biosolutions). The phosphorylation ratio was calculated and represented as fold changes of indicated phosphoproteins after H2O2 treatment normalized to total protein expression (upper panel). Total protein quantification is shown (lower panel). (b) HT22 cells were exposed to H2O2 with or without various concentrations of AAL extract (0, 12.5, 25, or 50 μg/mL) for 6 h. Cell lysates were prepared from HT22 cells and equal amounts of protein were subjected to Western blotting using anti-phospho-CaMK2 β/ν/δ, GRK2, EGFR, Myc, FER, caveolin-1, NFκB p65, and MLRN 2 antibodies to validate the Ab microarray. (c) Protein extracts were prepared from hippocampal tissues in an Aβ-induced AD mouse model. Vehicle or various concentrations of AAL extract (50, 100, or 200 mg/kg) were administered to Aβ mice for 23 days. Western blotting was performed for anti-phospho-EGFR and phospho-GRK2. The validity of the two phospho-antibodies was determined using pre-stained protein marker (Bio-Rad). GAPDH was used as an internal control. Shown blots are representative results from three independent experiments.
    Phospho Specific Antibody Microarray Slide, supplied by Full Moon BioSystems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microarray+slides/pmc06963381-315-39-43?v=Full+Moon+BioSystems
    Average 90 stars, based on 1 article reviews
    phospho-specific antibody microarray slide - by Bioz Stars, 2026-08
    90/100 stars

    Images

    1) Product Images from "Annona atemoya leaf extract ameliorates cognitive impairment in amyloid-β injected Alzheimer’s disease-like mouse model"

    Article Title: Annona atemoya leaf extract ameliorates cognitive impairment in amyloid-β injected Alzheimer’s disease-like mouse model

    Journal: Experimental Biology and Medicine

    doi: 10.1177/1535370219886269

    Molecular mechanisms responsible for anti-AD effects of AAL extract. (a) HT22 cells were exposed to H2O2 in the absence or presence of AAL extract (50 μg/mL) for 6 h. Antibody microarray assay was performed using the phospho-specific antibody microarray slide (Full Moon BioSystems). For data acquisition, GenePix 4100A scanner (Axon Instrument, USA) was used. The normalization data were analyzed using Genowiz 4.0™ (Ocimum Biosolutions). The phosphorylation ratio was calculated and represented as fold changes of indicated phosphoproteins after H2O2 treatment normalized to total protein expression (upper panel). Total protein quantification is shown (lower panel). (b) HT22 cells were exposed to H2O2 with or without various concentrations of AAL extract (0, 12.5, 25, or 50 μg/mL) for 6 h. Cell lysates were prepared from HT22 cells and equal amounts of protein were subjected to Western blotting using anti-phospho-CaMK2 β/ν/δ, GRK2, EGFR, Myc, FER, caveolin-1, NFκB p65, and MLRN 2 antibodies to validate the Ab microarray. (c) Protein extracts were prepared from hippocampal tissues in an Aβ-induced AD mouse model. Vehicle or various concentrations of AAL extract (50, 100, or 200 mg/kg) were administered to Aβ mice for 23 days. Western blotting was performed for anti-phospho-EGFR and phospho-GRK2. The validity of the two phospho-antibodies was determined using pre-stained protein marker (Bio-Rad). GAPDH was used as an internal control. Shown blots are representative results from three independent experiments.
    Figure Legend Snippet: Molecular mechanisms responsible for anti-AD effects of AAL extract. (a) HT22 cells were exposed to H2O2 in the absence or presence of AAL extract (50 μg/mL) for 6 h. Antibody microarray assay was performed using the phospho-specific antibody microarray slide (Full Moon BioSystems). For data acquisition, GenePix 4100A scanner (Axon Instrument, USA) was used. The normalization data were analyzed using Genowiz 4.0™ (Ocimum Biosolutions). The phosphorylation ratio was calculated and represented as fold changes of indicated phosphoproteins after H2O2 treatment normalized to total protein expression (upper panel). Total protein quantification is shown (lower panel). (b) HT22 cells were exposed to H2O2 with or without various concentrations of AAL extract (0, 12.5, 25, or 50 μg/mL) for 6 h. Cell lysates were prepared from HT22 cells and equal amounts of protein were subjected to Western blotting using anti-phospho-CaMK2 β/ν/δ, GRK2, EGFR, Myc, FER, caveolin-1, NFκB p65, and MLRN 2 antibodies to validate the Ab microarray. (c) Protein extracts were prepared from hippocampal tissues in an Aβ-induced AD mouse model. Vehicle or various concentrations of AAL extract (50, 100, or 200 mg/kg) were administered to Aβ mice for 23 days. Western blotting was performed for anti-phospho-EGFR and phospho-GRK2. The validity of the two phospho-antibodies was determined using pre-stained protein marker (Bio-Rad). GAPDH was used as an internal control. Shown blots are representative results from three independent experiments.

    Techniques Used: Microarray, Phospho-proteomics, Expressing, Western Blot, Staining, Marker, Control



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    Image Search Results


    Mean microarray antigen spot intensities from TB-free badger serum samples at two time points following experimental infection. White bars represent TB-free sera; gray bars represent TB-infected sera collected at ( a ) 8 weeks and ( b ) 12–13 weeks post-infection. Mean microarray spot intensities (log 2 -normalized) are shown with 95% confidence interval error bars. Paired-sample t -tests were carried out comparing pre- and post-infection mean spot intensities. Antigens were ranked by BH-adjusted P -values, and the 10 most differential antigens (defined as those with the lowest BH-adjusted P -values) for each comparison group are shown. Significance levels: ns: P ≥ 0.05; *: P < 0.05; **: P < 0.01; and ***: P < 0.001.

    Journal: Journal of Clinical Microbiology

    Article Title: Proteome microarray-guided identification of mycobacterial antigens and ELISA-based peptide mapping for improved serological detection of Mycobacterium bovis infection in European badgers

    doi: 10.1128/jcm.01260-25

    Figure Lengend Snippet: Mean microarray antigen spot intensities from TB-free badger serum samples at two time points following experimental infection. White bars represent TB-free sera; gray bars represent TB-infected sera collected at ( a ) 8 weeks and ( b ) 12–13 weeks post-infection. Mean microarray spot intensities (log 2 -normalized) are shown with 95% confidence interval error bars. Paired-sample t -tests were carried out comparing pre- and post-infection mean spot intensities. Antigens were ranked by BH-adjusted P -values, and the 10 most differential antigens (defined as those with the lowest BH-adjusted P -values) for each comparison group are shown. Significance levels: ns: P ≥ 0.05; *: P < 0.05; **: P < 0.01; and ***: P < 0.001.

    Article Snippet: Translated proteins and IVTT controls were printed onto custom nitrocellulose-coated microarray slides (Grace Bio-Labs, Bend, OR, USA) using a GeneMachines OmniGrid Accent Microarray Printer.

    Techniques: Microarray, Infection, Comparison

    Mean microarray antigen spot intensities from TB-infected and BCG-vaccinated badger serum samples. Gray bars represent sera from TB-infected badgers collected 12–13 weeks after experimental infection; white bars represent sera from badgers that received ( a ) a single BCG vaccination or ( b ) two BCG vaccinations. Mean spot intensities (log₂-normalized) are shown with 95% confidence interval error bars. Independent-samples t -tests were used to compare antigen intensities between TB-infected and BCG-vaccinated groups. Antigens were ranked by BH-adjusted P -values, and the 10 most differential antigens (defined as those with the lowest BH-adjusted P -values) for each comparison group are shown; ***: P < 0.001.

    Journal: Journal of Clinical Microbiology

    Article Title: Proteome microarray-guided identification of mycobacterial antigens and ELISA-based peptide mapping for improved serological detection of Mycobacterium bovis infection in European badgers

    doi: 10.1128/jcm.01260-25

    Figure Lengend Snippet: Mean microarray antigen spot intensities from TB-infected and BCG-vaccinated badger serum samples. Gray bars represent sera from TB-infected badgers collected 12–13 weeks after experimental infection; white bars represent sera from badgers that received ( a ) a single BCG vaccination or ( b ) two BCG vaccinations. Mean spot intensities (log₂-normalized) are shown with 95% confidence interval error bars. Independent-samples t -tests were used to compare antigen intensities between TB-infected and BCG-vaccinated groups. Antigens were ranked by BH-adjusted P -values, and the 10 most differential antigens (defined as those with the lowest BH-adjusted P -values) for each comparison group are shown; ***: P < 0.001.

    Article Snippet: Translated proteins and IVTT controls were printed onto custom nitrocellulose-coated microarray slides (Grace Bio-Labs, Bend, OR, USA) using a GeneMachines OmniGrid Accent Microarray Printer.

    Techniques: Microarray, Infection, Comparison