Journal: Journal of Clinical Microbiology
Article Title: Proteome microarray-guided identification of mycobacterial antigens and ELISA-based peptide mapping for improved serological detection of Mycobacterium bovis infection in European badgers
doi: 10.1128/jcm.01260-25
Figure Lengend Snippet: Mean microarray antigen spot intensities from TB-free badger serum samples at two time points following experimental infection. White bars represent TB-free sera; gray bars represent TB-infected sera collected at ( a ) 8 weeks and ( b ) 12–13 weeks post-infection. Mean microarray spot intensities (log 2 -normalized) are shown with 95% confidence interval error bars. Paired-sample t -tests were carried out comparing pre- and post-infection mean spot intensities. Antigens were ranked by BH-adjusted P -values, and the 10 most differential antigens (defined as those with the lowest BH-adjusted P -values) for each comparison group are shown. Significance levels: ns: P ≥ 0.05; *: P < 0.05; **: P < 0.01; and ***: P < 0.001.
Article Snippet: Translated proteins and IVTT controls were printed onto custom nitrocellulose-coated microarray slides (Grace Bio-Labs, Bend, OR, USA) using a GeneMachines OmniGrid Accent Microarray Printer.
Techniques: Microarray, Infection, Comparison