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LC Sciences mirna microarray service
Mirna Microarray Service, supplied by LC Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microarray+service/mirna+microarray+service/pm23355957-56-13-8
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Microarray:

Article Title: microRNA-140 targets RALA and regulates chondrogenic differentiation of human mesenchymal stem cells by translational enhancement of SOX9 and ACAN.
Article Snippet: Lesions of articular cartilage do not heal spontaneously.. One treatment strategy would be to make cartilage in the laboratory by directed chondrogenic differentiation of mesenchymal stem cells (MSCs).. To promote our understanding of the molecular control of chondrogenesis, we have compared the changes in microRNAs (miRNAs) during in vitro chondrogenesis of MSCs with those observed in uncultured and dedifferentiated articular chondrocytes (ACs).

Article Title: Genome-Scale Mapping of MicroRNA Signatures in Human Embryonic Stem Cell Neurogenesis
Article Snippet: .. The human miRNA expression profiling was done and the microarray data analysis from at least two biological replicate sets (e.g., WA01 and WA09) was provided by LC Sciences (Huston, TX, www.lcsciences.com ) as part of miRNA microarray service. ..

Article Title: Preeclampsia Up-Regulates Angiogenesis-Associated MicroRNA ( i.e ., miR-17, -20a, and -20b) That Target Ephrin-B2 and EPHB4 in Human Placenta
Article Snippet: For the transfected cells, total RNA were extracted using Trizol reagent (Invitrogen, Carlsbad, CA) following the manufacturer's protocol. .. The differential miRNA expression profile was analyzed by a miRNA microarray service provider (LC Sciences, Houston, TX) on three pairs of control and PE samples. ..

Article Title: MicroRNA expression profile in Lieber-DeCarli diet-induced alcoholic and methinine choline deficient (MCD) diet-induced non-alcoholic steatohepatitis models in mice
Article Snippet: Cytokine Measurements Serum TNFα level was determined using the Pierce SearchLight Multiplex Cytokine array (Pierce Biotechnology, Inc., Woburn, MA). microRNA analysis Total RNA was isolated from snap-frozen livers with organic and solid-phase extraction using mir Vana miRNA Isolation Kit, which allows for miRNAs preservation (Applied Biosystems/Ambion, Austin, TX, catalog #1560), and quantified by spectrophotometry (BioPhotometer, Eppendorf, Westbury, NY). .. Total liver RNA (5 μg) samples were analyzed for miRNA profile at LC Sciences LLC (Houston, TX) using miRNA Microarray Service. ..

Article Title: High-Throughput Sequencing Reveals Differential Expression of miRNAs in Intestine from Sea Cucumber during Aestivation
Article Snippet: .. After confirming their integrity, the total RNA was sent to LC Sciences (Hangzhou, China) to be processed using their miRNA Microarray Service. .. Each chip produced contained probes for 341 sea cucumber miRNAs from Solexa sequencing, 546 sea squirt Ciona intestinalis miRNAs, 101 acorn worm Saccoglossus kowalevskii miRNAs and 50 sea urchin S. purpuratus miRNAs from miRBase v 19.0.

Article Title: Large-scale identification and comparative analysis of miRNA expression profile in the respiratory tree of the sea cucumber Apostichopus japonicus during aestivation.
Article Snippet: Contents lists available at ScienceDirect Marine Genomics j ourna l homepage: www.e lsev ie r .com/ locate /margen Short communication Large-scale identification and comparative analysis of miRNA expression profile in the respiratory tree of the sea cucumber Apostichopus japonicus during aestivation Muyan Chen a,⁎, Kenneth B. Storey b a Fisheries College, Ocean University of China, Qingdao, PR China b Institute of Biochemistry, Carleton University, 1125 Colonel By Drive, Ottawa, ON K1S 5B6, Canada ⁎ Corresponding author.. E-mail address: chenmuyan@gmail.com (M. Chen).. 1874-7787/$ – see front matter © 2014 Elsevier B.V. All ri http://dx.doi.org/10.1016/j.margen.2014.01.002 a b s t r a c t a r t i c l e i n f o Article history: Received 19 November 2013 Received in revised form 7 January 2014 Accepted 8 January 2014 Keywords: miRNA Sea cucumber Aestivation Respiratory tree Solexa deep sequencing The sea cucumber Apostichopus japonicuswithstands high water temperatures in the summer by suppressing its metabolic rate and entering a state of aestivation.

Expressing:

Article Title: Genome-Scale Mapping of MicroRNA Signatures in Human Embryonic Stem Cell Neurogenesis
Article Snippet: .. The human miRNA expression profiling was done and the microarray data analysis from at least two biological replicate sets (e.g., WA01 and WA09) was provided by LC Sciences (Huston, TX, www.lcsciences.com ) as part of miRNA microarray service. ..

Article Title: Preeclampsia Up-Regulates Angiogenesis-Associated MicroRNA ( i.e ., miR-17, -20a, and -20b) That Target Ephrin-B2 and EPHB4 in Human Placenta
Article Snippet: For the transfected cells, total RNA were extracted using Trizol reagent (Invitrogen, Carlsbad, CA) following the manufacturer's protocol. .. The differential miRNA expression profile was analyzed by a miRNA microarray service provider (LC Sciences, Houston, TX) on three pairs of control and PE samples. ..

Article Title: Comparative profiling of miRNA expression of lung adenocarcinoma cells in two-dimensional and three-dimensional cultures
Article Snippet: .. The miRNA expression profiling service was provided by LC Sciences (Houston, TX) using μParaflo® technology and proprietary probe hybridization (Cy3 and Cy5 dendrimer dyes) that enables highly sensitive and specific direct detection of mouse miRNAs. ..

other:

Article Title: Extracellular vesicles extracted from young donor serum attenuate inflammaging via partially rejuvenating aged T‐cell immunotolerance
Article Snippet: In addition, we checked miRNA profiles of serum EVs extracted from young and aged mice with microarray of MusmusculusmiRBase version-21 array chips,where each chip contained 1900 unique mature miRNA probes (miRNA microarray service via LC Sciences).

Control:

Article Title: Preeclampsia Up-Regulates Angiogenesis-Associated MicroRNA ( i.e ., miR-17, -20a, and -20b) That Target Ephrin-B2 and EPHB4 in Human Placenta
Article Snippet: For the transfected cells, total RNA were extracted using Trizol reagent (Invitrogen, Carlsbad, CA) following the manufacturer's protocol. .. The differential miRNA expression profile was analyzed by a miRNA microarray service provider (LC Sciences, Houston, TX) on three pairs of control and PE samples. ..

Hybridization:

Article Title: Comparative profiling of miRNA expression of lung adenocarcinoma cells in two-dimensional and three-dimensional cultures
Article Snippet: .. The miRNA expression profiling service was provided by LC Sciences (Houston, TX) using μParaflo® technology and proprietary probe hybridization (Cy3 and Cy5 dendrimer dyes) that enables highly sensitive and specific direct detection of mouse miRNAs. ..



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CircRNA profiles in BALF EVs, serum EVs and BALF cells. Three mice were used in this experiment. BALF and serum were collected and pooled after exposure to PBS or LPS (1µg/mouse) or HCl (0.1N) for 24h. EVs were separated using ultracentrifugation as previously described . RNA was isolated from the EVs and subjected to circRNA <t>microarrays</t> by Arraystar Inc (Rockville, MD). Heatmap of the circRNA arrays in EVs obtained from BALF in the absence and presence of noxious stimuli. (A) BALF EVs obtained from mice that were exposed to PBS or LPS. (B) BALF EVs obtained from mice that were exposed to PBS or HCL. (C) the changes of EV-cargo circRNA profiles between non-infectious stimuli (HCL) and endotoxin (LPS) were expressed in Log2 fold change. (D) A representative picture showing the histological changes was presented here. The inflammatory score was determined by a double-blinded examiner using five lung sections per mouse, and three mice per treatment. The mean value is graphed and presented. ***p<0.05.
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CircRNA profiles in BALF EVs, serum EVs and BALF cells. Three mice were used in this experiment. BALF and serum were collected and pooled after exposure to PBS or LPS (1µg/mouse) or HCl (0.1N) for 24h. EVs were separated using ultracentrifugation as previously described . RNA was isolated from the EVs and subjected to circRNA <t>microarrays</t> by Arraystar Inc (Rockville, MD). Heatmap of the circRNA arrays in EVs obtained from BALF in the absence and presence of noxious stimuli. (A) BALF EVs obtained from mice that were exposed to PBS or LPS. (B) BALF EVs obtained from mice that were exposed to PBS or HCL. (C) the changes of EV-cargo circRNA profiles between non-infectious stimuli (HCL) and endotoxin (LPS) were expressed in Log2 fold change. (D) A representative picture showing the histological changes was presented here. The inflammatory score was determined by a double-blinded examiner using five lung sections per mouse, and three mice per treatment. The mean value is graphed and presented. ***p<0.05.
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CircRNA profiles in BALF EVs, serum EVs and BALF cells. Three mice were used in this experiment. BALF and serum were collected and pooled after exposure to PBS or LPS (1µg/mouse) or HCl (0.1N) for 24h. EVs were separated using ultracentrifugation as previously described . RNA was isolated from the EVs and subjected to circRNA <t>microarrays</t> by Arraystar Inc (Rockville, MD). Heatmap of the circRNA arrays in EVs obtained from BALF in the absence and presence of noxious stimuli. (A) BALF EVs obtained from mice that were exposed to PBS or LPS. (B) BALF EVs obtained from mice that were exposed to PBS or HCL. (C) the changes of EV-cargo circRNA profiles between non-infectious stimuli (HCL) and endotoxin (LPS) were expressed in Log2 fold change. (D) A representative picture showing the histological changes was presented here. The inflammatory score was determined by a double-blinded examiner using five lung sections per mouse, and three mice per treatment. The mean value is graphed and presented. ***p<0.05.
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CircRNA profiles in BALF EVs, serum EVs and BALF cells. Three mice were used in this experiment. BALF and serum were collected and pooled after exposure to PBS or LPS (1µg/mouse) or HCl (0.1N) for 24h. EVs were separated using ultracentrifugation as previously described . RNA was isolated from the EVs and subjected to circRNA microarrays by Arraystar Inc (Rockville, MD). Heatmap of the circRNA arrays in EVs obtained from BALF in the absence and presence of noxious stimuli. (A) BALF EVs obtained from mice that were exposed to PBS or LPS. (B) BALF EVs obtained from mice that were exposed to PBS or HCL. (C) the changes of EV-cargo circRNA profiles between non-infectious stimuli (HCL) and endotoxin (LPS) were expressed in Log2 fold change. (D) A representative picture showing the histological changes was presented here. The inflammatory score was determined by a double-blinded examiner using five lung sections per mouse, and three mice per treatment. The mean value is graphed and presented. ***p<0.05.

Journal: Frontiers in Immunology

Article Title: Altered circular RNA expressions in extracellular vesicles from bronchoalveolar lavage fluids in mice after bacterial infections

doi: 10.3389/fimmu.2024.1354676

Figure Lengend Snippet: CircRNA profiles in BALF EVs, serum EVs and BALF cells. Three mice were used in this experiment. BALF and serum were collected and pooled after exposure to PBS or LPS (1µg/mouse) or HCl (0.1N) for 24h. EVs were separated using ultracentrifugation as previously described . RNA was isolated from the EVs and subjected to circRNA microarrays by Arraystar Inc (Rockville, MD). Heatmap of the circRNA arrays in EVs obtained from BALF in the absence and presence of noxious stimuli. (A) BALF EVs obtained from mice that were exposed to PBS or LPS. (B) BALF EVs obtained from mice that were exposed to PBS or HCL. (C) the changes of EV-cargo circRNA profiles between non-infectious stimuli (HCL) and endotoxin (LPS) were expressed in Log2 fold change. (D) A representative picture showing the histological changes was presented here. The inflammatory score was determined by a double-blinded examiner using five lung sections per mouse, and three mice per treatment. The mean value is graphed and presented. ***p<0.05.

Article Snippet: For the circRNA profiling using microarrays, we used the service from Arraystar (Rockville, MD).

Techniques: Isolation