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microarray screening  (LC Sciences)

 
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    Structured Review

    LC Sciences microarray screening
    Microarray Screening, supplied by LC Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microarray+screens/microarray+screening/pm35020000-37-32-37
    Average 90 stars, based on 1 article reviews
    microarray screening - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Isolation:

    Article Title: MicroRNA-181c-5p modulates phagocytosis efficiency in bone marrow-derived macrophages.
    Article Snippet: .. RAW 264.7 cells were exposed to apoptotic H9c2 cells (apoptosis through UV exposure, 10 min) for 2 h. Total RNA was isolated from the control and apoptotic H9c2-treated macrophages and sent for microarray screening and bioinformatic analysis (LC Sciences, LLC, TX, USA). ..

    Control:

    Article Title: MicroRNA-181c-5p modulates phagocytosis efficiency in bone marrow-derived macrophages.
    Article Snippet: .. RAW 264.7 cells were exposed to apoptotic H9c2 cells (apoptosis through UV exposure, 10 min) for 2 h. Total RNA was isolated from the control and apoptotic H9c2-treated macrophages and sent for microarray screening and bioinformatic analysis (LC Sciences, LLC, TX, USA). ..

    Microarray:

    Article Title: MicroRNA-181c-5p modulates phagocytosis efficiency in bone marrow-derived macrophages.
    Article Snippet: .. RAW 264.7 cells were exposed to apoptotic H9c2 cells (apoptosis through UV exposure, 10 min) for 2 h. Total RNA was isolated from the control and apoptotic H9c2-treated macrophages and sent for microarray screening and bioinformatic analysis (LC Sciences, LLC, TX, USA). ..



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    Proteome and peptide screens were used to evaluate potential off-target binding of affinity-purified p4796kb antibodies. a Manhattan plot displays results from the HuProt Human Proteome Microarray screen of antibody binding. Colors represent each block from the array, containing approximately 1000 proteins each. Spots represent individual protein intensity score from array. Hits are annotated with protein names. b Manhattan plot from peptide counter screen of 21 putative hits from proteome screen. Overlapping 15mer peptides were synthesized and printed on arrays. Spots represent individual peptide intensity scores from array. Peptide sequences of hits are displayed. Sera from guinea pigs immunized with p4796kb were tested for binding to putative hits from screens ( c ) and calcitonin family ( d ) measured by ELISA. Pre-immune sera were used as controls. Data are presented as means +/− SEM; n = 3. *** p < 0.0001.

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    Figure Lengend Snippet: Proteome and peptide screens were used to evaluate potential off-target binding of affinity-purified p4796kb antibodies. a Manhattan plot displays results from the HuProt Human Proteome Microarray screen of antibody binding. Colors represent each block from the array, containing approximately 1000 proteins each. Spots represent individual protein intensity score from array. Hits are annotated with protein names. b Manhattan plot from peptide counter screen of 21 putative hits from proteome screen. Overlapping 15mer peptides were synthesized and printed on arrays. Spots represent individual peptide intensity scores from array. Peptide sequences of hits are displayed. Sera from guinea pigs immunized with p4796kb were tested for binding to putative hits from screens ( c ) and calcitonin family ( d ) measured by ELISA. Pre-immune sera were used as controls. Data are presented as means +/− SEM; n = 3. *** p < 0.0001.

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    Techniques: Binding Assay, Affinity Purification, Microarray, Blocking Assay, Synthesized, Enzyme-linked Immunosorbent Assay