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dna microarray data analysis raw data  (Thermo Fisher)


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    Structured Review

    Thermo Fisher dna microarray data analysis raw data
    Validation of <t> DNA </t> <t> Microarray </t> Results by qPCR
    Dna Microarray Data Analysis Raw Data, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microarray+raw+data+analysis/pmc02869047-101-0-17?v=Thermo+Fisher
    Average 99 stars, based on 1 article reviews
    dna microarray data analysis raw data - by Bioz Stars, 2026-08
    99/100 stars

    Images

    1) Product Images from "Perimysial Fibroblasts of Extraocular Muscle, as Unique as the Muscle Fibers"

    Article Title: Perimysial Fibroblasts of Extraocular Muscle, as Unique as the Muscle Fibers

    Journal: Investigative Ophthalmology & Visual Science

    doi: 10.1167/iovs.08-2857

    Validation of DNA Microarray Results by qPCR
    Figure Legend Snippet: Validation of DNA Microarray Results by qPCR

    Techniques Used: Biomarker Discovery, Microarray



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    Image Search Results


    Microarray analysis using mRNA from p16−/− BMDM compared to p16+/+ BMDM showed (A) decreased mRNA expression of classically activated macrophages-associated genes and (B) increased mRNA expression of alternatively activated macrophages-associated genes. Data is expressed as fold change relative to p16+/+ BMDM. (C) Differential gene expression in p16−/− BMDM relative to p16+/+ BMDM was correlated with the changes induced in IL-4-induced p16+/+ AAMφ. The figure shows 2log values of the probesets significantly (p<0.05) regulated only in p16−/− BMDM (red dots), only in IL-4-polarized p16+/+ AAMφ (green dots) and by both conditions (blue dots), compared to p16+/+ BMDM. The X-axis represents differences in gene expression induced by IL-4, whereas the Y-axis represents the effect of p16INKa-deficiency. These comparisons are depicted in the schematic representation of the protocol in the corresponding colors. Pearson Correlation analysis was done for probesets differentially expressed by both conditions (blue). (D) Heat map of p16+/+ BMDM, p16−/− BMDM, IL-4-polarized p16+/+ and p16−/− AAMφ gene expression profiles. Colors fluctuate from blue (poorly expressed) to green (intermediate expression) and yellow (high expression). Additional information regarding gene description, fold induction, and p-value can be found in Table S3.

    Journal: Blood

    Article Title: p16 INK4a deficiency promotes IL-4-induced polarization and inhibits proinflammatory signaling in macrophages

    doi: 10.1182/blood-2010-10-313106

    Figure Lengend Snippet: Microarray analysis using mRNA from p16−/− BMDM compared to p16+/+ BMDM showed (A) decreased mRNA expression of classically activated macrophages-associated genes and (B) increased mRNA expression of alternatively activated macrophages-associated genes. Data is expressed as fold change relative to p16+/+ BMDM. (C) Differential gene expression in p16−/− BMDM relative to p16+/+ BMDM was correlated with the changes induced in IL-4-induced p16+/+ AAMφ. The figure shows 2log values of the probesets significantly (p<0.05) regulated only in p16−/− BMDM (red dots), only in IL-4-polarized p16+/+ AAMφ (green dots) and by both conditions (blue dots), compared to p16+/+ BMDM. The X-axis represents differences in gene expression induced by IL-4, whereas the Y-axis represents the effect of p16INKa-deficiency. These comparisons are depicted in the schematic representation of the protocol in the corresponding colors. Pearson Correlation analysis was done for probesets differentially expressed by both conditions (blue). (D) Heat map of p16+/+ BMDM, p16−/− BMDM, IL-4-polarized p16+/+ and p16−/− AAMφ gene expression profiles. Colors fluctuate from blue (poorly expressed) to green (intermediate expression) and yellow (high expression). Additional information regarding gene description, fold induction, and p-value can be found in Table S3.

    Article Snippet: We thank E. Vallez for mouse breeding, J. Brozek (Genfit SA, Loos, France) for microarray raw data analysis, T. Coevoet, N. Jouy and A. Lucas for technical assistance.

    Techniques: Microarray, Expressing, Gene Expression

    Representation of the relative microarray intensity values from a selection of down-regulated genes in p16+/+ and p16−/− BMDM with or without polarization (AAMφ) by 15 ng/mL IL-4 from day 0 of differentiation. Statistically significant differences are indicated (a: p<0.05 compared to p16+/+ BMDM; b: p<0.05 compared to p16−/− BMDM; c: p<0.05 compared to p16+/+ AAMφ.)

    Journal: Blood

    Article Title: p16 INK4a deficiency promotes IL-4-induced polarization and inhibits proinflammatory signaling in macrophages

    doi: 10.1182/blood-2010-10-313106

    Figure Lengend Snippet: Representation of the relative microarray intensity values from a selection of down-regulated genes in p16+/+ and p16−/− BMDM with or without polarization (AAMφ) by 15 ng/mL IL-4 from day 0 of differentiation. Statistically significant differences are indicated (a: p<0.05 compared to p16+/+ BMDM; b: p<0.05 compared to p16−/− BMDM; c: p<0.05 compared to p16+/+ AAMφ.)

    Article Snippet: We thank E. Vallez for mouse breeding, J. Brozek (Genfit SA, Loos, France) for microarray raw data analysis, T. Coevoet, N. Jouy and A. Lucas for technical assistance.

    Techniques: Microarray, Selection

    Validation of DNA Microarray Results by qPCR

    Journal: Investigative Ophthalmology & Visual Science

    Article Title: Perimysial Fibroblasts of Extraocular Muscle, as Unique as the Muscle Fibers

    doi: 10.1167/iovs.08-2857

    Figure Lengend Snippet: Validation of DNA Microarray Results by qPCR

    Article Snippet: DNA Microarray Data Analysis Raw data from microarray scans were analyzed with microarray analysis software (GCOS 2.0; Affymetrix).

    Techniques: Biomarker Discovery, Microarray