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Arraystar inc human gene expression microarray
Human Gene Expression Microarray, supplied by Arraystar inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microarray+profiling+of+human+gene+expression/human+gene+expression+microarray/pm39881131-132-35-45
Average 90 stars, based on 1 article reviews
human gene expression microarray - by Bioz Stars, 2026-10
90/100 stars

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Labeling:

Article Title: KIF26B, a novel oncogene, promotes proliferation and metastasis by activating the VEGF pathway in gastric cancer.
Article Snippet: Tumor metastasis is the main reason of cancer-related death for gastric cancer (GC) patients and gene expression microarray data indicate that kinesin family member 26B (KIF26B) is one of the most upregulated genes in metastatic GC samples.. Specifically, KIF26B expression was upregulated in a stepwise manner from non-tumorous gastric mucosa, primary GC tissues without metastasis, via primary GC tissues with metastasis, to secondary lymph node metastatic (LNM) foci.. Increased expression of KIF26B was correlated with tumor size, positive LNM or distant metastases and poor prognosis.

Gene Expression:

Article Title: KIF26B, a novel oncogene, promotes proliferation and metastasis by activating the VEGF pathway in gastric cancer.
Article Snippet: Tumor metastasis is the main reason of cancer-related death for gastric cancer (GC) patients and gene expression microarray data indicate that kinesin family member 26B (KIF26B) is one of the most upregulated genes in metastatic GC samples.. Specifically, KIF26B expression was upregulated in a stepwise manner from non-tumorous gastric mucosa, primary GC tissues without metastasis, via primary GC tissues with metastasis, to secondary lymph node metastatic (LNM) foci.. Increased expression of KIF26B was correlated with tumor size, positive LNM or distant metastases and poor prognosis.

Article Title: LncRNA PCAT1 and its genetic variant rs1902432 are associated with prostate cancer risk
Article Snippet: Total RNA was extracted from DU145 PCa cells using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer's instructions. .. The mRNA of three pairs of DU145 cells transfected with PCAT1 overexpression vector or corresponding NC vector was profiled with Arraystar Human Gene Expression Array. ..

Article Title: SP-1-activated LINC01016 overexpression promotes gastric cancer invasion and metastasis through inhibiting EIF4A3-mediated MMP9 mRNA decay
Article Snippet: .. First, differential gene expression analysis of lncRNAs was performed between five cases of gastric cancers with LNM and another five cases without LNM using the Human Gene Expression Microarray (Human 8×60 K LncRNA Microarray v2.0; Arraystar, Rockville, USA). ..

Article Title: SP-1-activated LINC01016 overexpression promotes gastric cancer invasion and metastasis through inhibiting EIF4A3-mediated MMP9 mRNA decay.
Article Snippet: .. RESULTS LINC01016 is upregulated in GC with lymph node metastasis First, differential gene expression analysis of lncRNAs was performed between five cases of gastric cancers with LNM and another five cases without LNM using the Human Gene Expression Microarray (Human 8×60 K LncRNA Microarray v2.0; Arraystar, Rockville, USA). ..

Article Title: LncRNA PCAT1 and its genetic variant rs1902432 are associated with prostate cancer risk
Article Snippet: .. Arraystar Human Gene Expression Array was conducted to assess the genome-wide profiling of gene expression from PCa cell DU145 transfected with PCAT1 overexpression vector or NC vector. ..

Article Title: Long noncoding RNA lnc-LEMGC combines with DNA-PKcs to suppress gastric cancer metastasis.
Article Snippet: Long non-coding RNAs (lncRNAs) play important roles in cancer development and progression; however, their contributions to gastric cancer metastasis remain largely unknown.. By lncRNA microarray screening, our study showed that 453 lncRNAs are dysregulated in gastric cancer tissues with or without lymph node metastasis, of which lnc-LEMGC ranks as one of the most significantly downregulated lncRNAs.. Lnc-LEMGC inhibited cell migration and invasion both in vitro and in vivo, by combining with protein DNA-PKcs.

Article Title: PIN1P1 is activated by CREB1 and promotes gastric cancer progression via interacting with YBX1 and upregulating PIN1.
Article Snippet: .. Differential lncRNA screening was performed using the Human Gene Expression Microarray (Human 8 × 60 K LncRNA Microarray v2.0; Arraystar). ..

Microarray:

Article Title: KIF26B, a novel oncogene, promotes proliferation and metastasis by activating the VEGF pathway in gastric cancer.
Article Snippet: Tumor metastasis is the main reason of cancer-related death for gastric cancer (GC) patients and gene expression microarray data indicate that kinesin family member 26B (KIF26B) is one of the most upregulated genes in metastatic GC samples.. Specifically, KIF26B expression was upregulated in a stepwise manner from non-tumorous gastric mucosa, primary GC tissues without metastasis, via primary GC tissues with metastasis, to secondary lymph node metastatic (LNM) foci.. Increased expression of KIF26B was correlated with tumor size, positive LNM or distant metastases and poor prognosis.

Article Title: SP-1-activated LINC01016 overexpression promotes gastric cancer invasion and metastasis through inhibiting EIF4A3-mediated MMP9 mRNA decay
Article Snippet: .. First, differential gene expression analysis of lncRNAs was performed between five cases of gastric cancers with LNM and another five cases without LNM using the Human Gene Expression Microarray (Human 8×60 K LncRNA Microarray v2.0; Arraystar, Rockville, USA). ..

Article Title: SP-1-activated LINC01016 overexpression promotes gastric cancer invasion and metastasis through inhibiting EIF4A3-mediated MMP9 mRNA decay.
Article Snippet: .. RESULTS LINC01016 is upregulated in GC with lymph node metastasis First, differential gene expression analysis of lncRNAs was performed between five cases of gastric cancers with LNM and another five cases without LNM using the Human Gene Expression Microarray (Human 8×60 K LncRNA Microarray v2.0; Arraystar, Rockville, USA). ..

Article Title: Long noncoding RNA lnc-LEMGC combines with DNA-PKcs to suppress gastric cancer metastasis.
Article Snippet: Long non-coding RNAs (lncRNAs) play important roles in cancer development and progression; however, their contributions to gastric cancer metastasis remain largely unknown.. By lncRNA microarray screening, our study showed that 453 lncRNAs are dysregulated in gastric cancer tissues with or without lymph node metastasis, of which lnc-LEMGC ranks as one of the most significantly downregulated lncRNAs.. Lnc-LEMGC inhibited cell migration and invasion both in vitro and in vivo, by combining with protein DNA-PKcs.

Article Title: PIN1P1 is activated by CREB1 and promotes gastric cancer progression via interacting with YBX1 and upregulating PIN1.
Article Snippet: .. Differential lncRNA screening was performed using the Human Gene Expression Microarray (Human 8 × 60 K LncRNA Microarray v2.0; Arraystar). ..

Transfection:

Article Title: LncRNA PCAT1 and its genetic variant rs1902432 are associated with prostate cancer risk
Article Snippet: Total RNA was extracted from DU145 PCa cells using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer's instructions. .. The mRNA of three pairs of DU145 cells transfected with PCAT1 overexpression vector or corresponding NC vector was profiled with Arraystar Human Gene Expression Array. ..

Article Title: LncRNA PCAT1 and its genetic variant rs1902432 are associated with prostate cancer risk
Article Snippet: .. Arraystar Human Gene Expression Array was conducted to assess the genome-wide profiling of gene expression from PCa cell DU145 transfected with PCAT1 overexpression vector or NC vector. ..

Over Expression:

Article Title: LncRNA PCAT1 and its genetic variant rs1902432 are associated with prostate cancer risk
Article Snippet: Total RNA was extracted from DU145 PCa cells using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer's instructions. .. The mRNA of three pairs of DU145 cells transfected with PCAT1 overexpression vector or corresponding NC vector was profiled with Arraystar Human Gene Expression Array. ..

Article Title: LncRNA PCAT1 and its genetic variant rs1902432 are associated with prostate cancer risk
Article Snippet: .. Arraystar Human Gene Expression Array was conducted to assess the genome-wide profiling of gene expression from PCa cell DU145 transfected with PCAT1 overexpression vector or NC vector. ..

Plasmid Preparation:

Article Title: LncRNA PCAT1 and its genetic variant rs1902432 are associated with prostate cancer risk
Article Snippet: Total RNA was extracted from DU145 PCa cells using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer's instructions. .. The mRNA of three pairs of DU145 cells transfected with PCAT1 overexpression vector or corresponding NC vector was profiled with Arraystar Human Gene Expression Array. ..

Article Title: LncRNA PCAT1 and its genetic variant rs1902432 are associated with prostate cancer risk
Article Snippet: .. Arraystar Human Gene Expression Array was conducted to assess the genome-wide profiling of gene expression from PCa cell DU145 transfected with PCAT1 overexpression vector or NC vector. ..

Genome Wide:

Article Title: LncRNA PCAT1 and its genetic variant rs1902432 are associated with prostate cancer risk
Article Snippet: .. Arraystar Human Gene Expression Array was conducted to assess the genome-wide profiling of gene expression from PCa cell DU145 transfected with PCAT1 overexpression vector or NC vector. ..



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Validation of genes identified using microarray. MCF7 and TAMR cells were transfected with silencer negative control siRNA, ATF2 siRNA1 and ATF2 siRNA2. qRT-PCR was carried out using Taqman primers for the genes indicated. Data analysis for each cell line was carried out relative to the siControl ( n = 3) and asterisks indicate the genes that were significantly changed after ATF2 knockdown (* p < 0.05, ** p < 0.005)

Journal: Breast Cancer Research : BCR

Article Title: Activating transcription factor-2 (ATF2) is a key determinant of resistance to endocrine treatment in an in vitro model of breast cancer

doi: 10.1186/s13058-020-01359-7

Figure Lengend Snippet: Validation of genes identified using microarray. MCF7 and TAMR cells were transfected with silencer negative control siRNA, ATF2 siRNA1 and ATF2 siRNA2. qRT-PCR was carried out using Taqman primers for the genes indicated. Data analysis for each cell line was carried out relative to the siControl ( n = 3) and asterisks indicate the genes that were significantly changed after ATF2 knockdown (* p < 0.05, ** p < 0.005)

Article Snippet: We also performed a microarray gene expression profiling (Illumina Human HT12_v4) to explore alterations in gene expression between MCF7 and TAMRs after ATF2 silencing and confirmed gene expression changes by quantitative RT-PCR.

Techniques: Biomarker Discovery, Microarray, Transfection, Negative Control, Quantitative RT-PCR, Knockdown