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dna microarray preparation, hybridization, processing, scanning, and analyses  (Filgen Inc)

 
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    Filgen Inc dna microarray preparation, hybridization, processing, scanning, and analyses
    Dna Microarray Preparation, Hybridization, Processing, Scanning, And Analyses, supplied by Filgen Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microarray+process+of+hybridization/microarray+analyses/pm20034649-285-3-11
    Average 90 stars, based on 1 article reviews
    dna microarray preparation, hybridization, processing, scanning, and analyses - by Bioz Stars, 2026-09
    90/100 stars

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    Staining:

    Article Title: Anti-Obesity Properties of Boesenbergia rotunda Rhizome Extract: Regulation of Inflammation, Lipid Metabolism, and Insulin Signaling in ob/ob Mice
    Article Snippet: .. Acknowledgments: We thank Kyodo Byori (Kobe, Japan) for staining the liver samples; Filgen Inc. (Nagoya, Aichi, Japan) for microarray analysis; AJI, Ritsumeikan Asia-Japan Research Organization, Ritsumeikan University; and the Peningkatan Kualitas Publikasi Ilmiah (PKPI) program (106.5/E4.4/KU/2023) from the Direktorat Riset, Teknologi, dan Pengabdian kepada Masyarakat (DRTPM), Indonesia, for supporting this research. ..

    Microarray:

    Article Title: Anti-Obesity Properties of Boesenbergia rotunda Rhizome Extract: Regulation of Inflammation, Lipid Metabolism, and Insulin Signaling in ob/ob Mice
    Article Snippet: .. Acknowledgments: We thank Kyodo Byori (Kobe, Japan) for staining the liver samples; Filgen Inc. (Nagoya, Aichi, Japan) for microarray analysis; AJI, Ritsumeikan Asia-Japan Research Organization, Ritsumeikan University; and the Peningkatan Kualitas Publikasi Ilmiah (PKPI) program (106.5/E4.4/KU/2023) from the Direktorat Riset, Teknologi, dan Pengabdian kepada Masyarakat (DRTPM), Indonesia, for supporting this research. ..

    Article Title: Shikonin changes the lipopolysaccharide-induced expression of inflammation-related genes in macrophages.
    Article Snippet: We aimed to find candidate molecules possibly involved in the anti-inflammatory activity of shikonin (active compound of ‘‘Shikon’’) by analyzing its effects on gene expression of lipopolysaccharide (LPS)-treated THP1 macrophages.. Polysome-associated mRNAs (those expected to be under translation: translatome) from cells treated with LPS alone (LPS: 5 lg/mL), shikonin alone (S: 100 nM), or LPS plus shikonin (LPS&S) for 3 h were analyzed by DNA microarray followed by detection of enriched pathways/gene ontologies using the tools of the STRING database.. Candidate genes in enriched pathways in the comparison of LPS&S cells vs. LPS cells were analyzed by reverse-transcription quantitative real-time PCR (RT-qPCR; 1, 2, and 3 h).

    Article Title: Spinal lipocalin 2 as a factor in the development of central post-stroke pain.
    Article Snippet: Central poststroke pain (CPSP) is a type of central neuropathic pain whose mechanisms remain unknown.. Recently, we showed that activated astrocytes and microglial cells are present in the spinal cord of CPSP model mice.. Activated glial cells exacerbate cerebral ischemic pathology by increasing the expression of inflammatory factors.

    Article Title: Comparison of the effects of pachymic acid, moronic acid and hydrocortisone on the polysome loading of RNAs in lipopolysaccharide-treated THP-1 macrophages.
    Article Snippet: We have proposed that analysis of ribosome-loaded mRNAs (i.e., the translatome) is useful for elucidation of pharmacological effects of phytocompounds in immune cells, regarding the involvement of post-transcriptional regulation mechanisms.. In the present study, we compared the effects of pachymic acid from Poria cocos fungus and moronic acid from propolis with those of hydrocortisone on the translatomes of THP-1 macrophages exposed to bacterial lipopolysaccharide (LPS) to find clues to their biological effects.. Polysome-associated RNAs collected from cells treated for 3 h with LPS plus each of the compounds were analyzed by DNA microarray followed by analyses of pathways/gene ontologies (GO).

    Article Title: Association between oxidative stress and microRNA expression pattern of ALS patients in the high-incidence area of the Kii Peninsula.
    Article Snippet: Amyotrophic lateral sclerosis (ALS) is a progressive degenerative disorder of the upper and lower motor neuron systems.. The high incidence of ALS in the southern part of the Kii Peninsula of Japan (K-ALS) was reported in the 1960s, but it has gradually decreased to the worldwide average.. Although causes of the high incidence of ALS in this area are unknown, our previous studies suggested that environmental factors, including essential mineral deficiency and increased metal-induced oxidative stress, play a role in its development.

    Article Title: Interleukin-33 delays recovery of mucosal inflammation via downregulation of homeostatic ABCG5/8 in the colon.
    Article Snippet: .. RNA samples were sent to Filgen (Aichi, Japan), where DNA microarray analyses were performed. ..

    Article Title: ANXA10 induction by interaction with tumor‐associated macrophages promotes the growth of esophageal squamous cell carcinoma
    Article Snippet: A cDNA microarray was performed using the GeneChip Human Gene 2.0 ST Array (Affymetrix, Santa Clara, CA, USA), containing 53617 probes characterized human genes and expressed sequence tags. .. A company (Filgen, Nagoya, Japan) conducted the DNA microarray analysis. ..

    Article Title: m 6 A demethylase ALKBH5 promotes tumor cell proliferation by destabilizing IGF2BPs target genes and worsens the prognosis of patients with non-small cell lung cancer
    Article Snippet: RNA samples were used for global gene expression profiling on human Clariom S Assay microarrays (Thermo Fisher Scientific, Wilmington, DE, USA), which include 24351 genes. .. All microarray analyses were entrusted to Filgen Inc. (Aichi, Japan). .. A total RNA quality control check was performed using a NanoDrop ND-1000 (Thermo Scientific) and an Agilent 2100 Bioanalyzer.

    Gene Expression:

    Article Title: Spinal lipocalin 2 as a factor in the development of central post-stroke pain.
    Article Snippet: Central poststroke pain (CPSP) is a type of central neuropathic pain whose mechanisms remain unknown.. Recently, we showed that activated astrocytes and microglial cells are present in the spinal cord of CPSP model mice.. Activated glial cells exacerbate cerebral ischemic pathology by increasing the expression of inflammatory factors.



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    circRNA_104797 is upregulated in BCa. A Heatmap illustrating the differential expression profiles of circRNAs between four matched sets of bladder cancer and adjacent normal tissues; B volcano plot representing the results of circRNA microarray analysis; C schematic diagram outlining the selection criteria for identifying candidate regulatory circRNAs enriched in bladder cancer; D comparative expression levels of circRNA_104797 across SV-HVC-1, T24, T24/CDDP, 5637, and 5637/CDDP cell lines; E Kaplan–Meier survival analysis depicting overall survival rates in bladder cancer patients stratified by low or high expression levels of circRNA_104797 ( n = 40)

    Journal: Cellular & Molecular Biology Letters

    Article Title: N 6 -methyladenosine-modified circ_104797 sustains cisplatin resistance in bladder cancer through acting as RNA sponges

    doi: 10.1186/s11658-024-00543-3

    Figure Lengend Snippet: circRNA_104797 is upregulated in BCa. A Heatmap illustrating the differential expression profiles of circRNAs between four matched sets of bladder cancer and adjacent normal tissues; B volcano plot representing the results of circRNA microarray analysis; C schematic diagram outlining the selection criteria for identifying candidate regulatory circRNAs enriched in bladder cancer; D comparative expression levels of circRNA_104797 across SV-HVC-1, T24, T24/CDDP, 5637, and 5637/CDDP cell lines; E Kaplan–Meier survival analysis depicting overall survival rates in bladder cancer patients stratified by low or high expression levels of circRNA_104797 ( n = 40)

    Article Snippet: The protocols outlined by Arraystar (Rockville, MD, USA) guided our sample preparation and microarray hybridization processes.

    Techniques: Quantitative Proteomics, Microarray, Selection, Expressing