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Thermo Fisher gene exp mafb hs00271378 s1

Expression of <t>MAFb</t> gene and protein in primary plasma cells and HMCLs. Affymetrix expression of MAFb mRNA was high in the GEP MF subgroup ( a ). Gene expression of 803 newly diagnosed MM patients was measured by U133 plus2.0 Affymetrix oligonucleotide microarray probe set 218559_s_at. The box plots indicate the middle of GEP signaling and dot lines with bars in black indicate quartiles. The cells were cultured in the growth mediate for 48 h and harvested for isolated RNA and MAFb mRNA in 27 HMCLs by qRT-PCR analysis as described in Material and methods ( b ). The cells were cultured in the growth mediate for 48 h and MAFb protein was measured in HMCLs by immunoblotting analysis described in Materials and Methods ( c ). MAFb protein in nuclei and cytoplasm of HMCLs ( d ) and MM PC from 4 patients ( e ) was determined with immunofluorescence staining with DAPI counterstaining. Images were taken with a fluorescence microscope with digital camera as described in supplemental data

Gene Exp Mafb Hs00271378 S1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microarray+probe+sets/pmc06035431-66-6-15?v=Thermo+Fisher
Average 86 stars, based on 1 article reviews

gene exp mafb hs00271378 s1 - by Bioz Stars, 2026-08

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1) Product Images from "MAFb protein confers intrinsic resistance to proteasome inhibitors in multiple myeloma"

Article Title: MAFb protein confers intrinsic resistance to proteasome inhibitors in multiple myeloma

Journal: BMC Cancer

doi: 10.1186/s12885-018-4602-4

Expression of MAFb gene and protein in primary plasma cells and HMCLs. Affymetrix expression of MAFb mRNA was high in the GEP MF subgroup ( a ). Gene expression of 803 newly diagnosed MM patients was measured by U133 plus2.0 Affymetrix oligonucleotide microarray probe set 218559_s_at. The box plots indicate the middle of GEP signaling and dot lines with bars in black indicate quartiles. The cells were cultured in the growth mediate for 48 h and harvested for isolated RNA and MAFb mRNA in 27 HMCLs by qRT-PCR analysis as described in Material and methods ( b ). The cells were cultured in the growth mediate for 48 h and MAFb protein was measured in HMCLs by immunoblotting analysis described in Materials and Methods ( c ). MAFb protein in nuclei and cytoplasm of HMCLs ( d ) and MM PC from 4 patients ( e ) was determined with immunofluorescence staining with DAPI counterstaining. Images were taken with a fluorescence microscope with digital camera as described in supplemental data

Figure Legend Snippet: Expression of MAFb gene and protein in primary plasma cells and HMCLs. Affymetrix expression of MAFb mRNA was high in the GEP MF subgroup ( a ). Gene expression of 803 newly diagnosed MM patients was measured by U133 plus2.0 Affymetrix oligonucleotide microarray probe set 218559_s_at. The box plots indicate the middle of GEP signaling and dot lines with bars in black indicate quartiles. The cells were cultured in the growth mediate for 48 h and harvested for isolated RNA and MAFb mRNA in 27 HMCLs by qRT-PCR analysis as described in Material and methods ( b ). The cells were cultured in the growth mediate for 48 h and MAFb protein was measured in HMCLs by immunoblotting analysis described in Materials and Methods ( c ). MAFb protein in nuclei and cytoplasm of HMCLs ( d ) and MM PC from 4 patients ( e ) was determined with immunofluorescence staining with DAPI counterstaining. Images were taken with a fluorescence microscope with digital camera as described in supplemental data

Techniques Used: Expressing, Microarray, Cell Culture, Isolation, Quantitative RT-PCR, Western Blot, Immunofluorescence, Staining, Fluorescence, Microscopy

High MAFb expression is associated with resistance to PIs. SACHI ( a ), H929 ( b ), EJM ( c ), XG2 ( d ), L363 ( e ), and OPM-2 ( f ) cells were seeded at 2 × 10^4 per well in 96-well plates in the presence of indicated concentrations of Bzb or CFZ for 48 h. Cell survival was measured by MTT Assay. Results are presented as mean ± SE ( n = 4). Data are representative of 3 separate experiments. HMCLs were treated with serial concentrations of Bzb (G) and CFZ (H) for 48 h and cell viability was measured by MTT assay. The IC50 of Bzb (G) and CFZ (H) were classified based on MAFb protein (+) or (−)

Figure Legend Snippet: High MAFb expression is associated with resistance to PIs. SACHI ( a ), H929 ( b ), EJM ( c ), XG2 ( d ), L363 ( e ), and OPM-2 ( f ) cells were seeded at 2 × 10^4 per well in 96-well plates in the presence of indicated concentrations of Bzb or CFZ for 48 h. Cell survival was measured by MTT Assay. Results are presented as mean ± SE ( n = 4). Data are representative of 3 separate experiments. HMCLs were treated with serial concentrations of Bzb (G) and CFZ (H) for 48 h and cell viability was measured by MTT assay. The IC50 of Bzb (G) and CFZ (H) were classified based on MAFb protein (+) or (−)

Techniques Used: Expressing, MTT Assay

Inhibition of GSK3 activity by SB216763 stabilized MAFb protein. HMCLs were treated with 5 μg/ml of CHX for serial indicated time points to inhibit de novo protein synthesis. The MAFb protein was determined by immunoblotting analysis using anti-MAFb. The membranes were striped and reblotted with Anti-β-actin ( a ). The half-life of MAFb protein was determined by autoradiographs analysis using Adobe Photoshop software and NIH image software ( b - f ). HMCLs were treated with or without a specific GSK3 inhibitor, SB216763, at a concentration of 5 μg/ml for indicated times. MAFb protein was determined by immunoblotting analysis using anti-MAFb antibody. The membranes were striped and reblotted with Anti-β-actin to indicate protein loading ( a ). The protein decay curve is as described in Fig. 3b (b-f)

Figure Legend Snippet: Inhibition of GSK3 activity by SB216763 stabilized MAFb protein. HMCLs were treated with 5 μg/ml of CHX for serial indicated time points to inhibit de novo protein synthesis. The MAFb protein was determined by immunoblotting analysis using anti-MAFb. The membranes were striped and reblotted with Anti-β-actin ( a ). The half-life of MAFb protein was determined by autoradiographs analysis using Adobe Photoshop software and NIH image software ( b - f ). HMCLs were treated with or without a specific GSK3 inhibitor, SB216763, at a concentration of 5 μg/ml for indicated times. MAFb protein was determined by immunoblotting analysis using anti-MAFb antibody. The membranes were striped and reblotted with Anti-β-actin to indicate protein loading ( a ). The protein decay curve is as described in Fig. 3b (b-f)

Techniques Used: Inhibition, Activity Assay, Western Blot, Software, Concentration Assay

Proteasome Inhibitors stabilize MAFb protein. The SACHI ( a ) and XG2 ( b ) cells were treated with indicated concentrations of Bzb or CFZ at the presence of 10 μg/ml of CHX for 6 h. MAFb protein lysate was analyzed as described in Fig. . SACHI ( c ) were treated with 20-nM of Bzb or CFZ for 12 h; XG-2, and EJM cells ( d ) were treated with indicated serial concentrations of Bzb and CFZ for 12 h, MAFb protein in nuclei and cytoplasm of the cells was determined by analysis of immunofluorescence staining with DAPI counterstaining to indicate the nucleus. Images were taken with a fluorescence microscope with digital camera as described in

Figure Legend Snippet: Proteasome Inhibitors stabilize MAFb protein. The SACHI ( a ) and XG2 ( b ) cells were treated with indicated concentrations of Bzb or CFZ at the presence of 10 μg/ml of CHX for 6 h. MAFb protein lysate was analyzed as described in Fig. . SACHI ( c ) were treated with 20-nM of Bzb or CFZ for 12 h; XG-2, and EJM cells ( d ) were treated with indicated serial concentrations of Bzb and CFZ for 12 h, MAFb protein in nuclei and cytoplasm of the cells was determined by analysis of immunofluorescence staining with DAPI counterstaining to indicate the nucleus. Images were taken with a fluorescence microscope with digital camera as described in

Techniques Used: Immunofluorescence, Staining, Fluorescence, Microscopy

Knockdown of MAFb restores sensitivity of myeloma to PIs. SACHI cells were infected with lentiviral expression system containing shRNA specific to MAFb gene (sh MAFb ) or shRNA containing scramble sequences (shCon) for 48 h. MAFb mRNA was measured by RT-qPCR analysis ( a ), MAFb protein in whole lysis buffer was analyzed by immunoblotting analysis ( b ) and in both cytoplasmic and in nucleus by immunofluorescent staining analysis ( c ) as described in Fig. 5d. MAFb target genes in sh MAFb and shCon cells were analyzed by qRT-PCR analysis ( d ). The cells were treated with indicated concentrations of Bzb ( e ) or CFZ ( f ) for 48 h and cell survival was measured by MTT assay. Results are presented as mean ± SE ( n = 4). Data are representative of 3 separate experiments. * P < 0 .01 versus control. ** P < 0 .001 versus control

Figure Legend Snippet: Knockdown of MAFb restores sensitivity of myeloma to PIs. SACHI cells were infected with lentiviral expression system containing shRNA specific to MAFb gene (sh MAFb ) or shRNA containing scramble sequences (shCon) for 48 h. MAFb mRNA was measured by RT-qPCR analysis ( a ), MAFb protein in whole lysis buffer was analyzed by immunoblotting analysis ( b ) and in both cytoplasmic and in nucleus by immunofluorescent staining analysis ( c ) as described in Fig. 5d. MAFb target genes in sh MAFb and shCon cells were analyzed by qRT-PCR analysis ( d ). The cells were treated with indicated concentrations of Bzb ( e ) or CFZ ( f ) for 48 h and cell survival was measured by MTT assay. Results are presented as mean ± SE ( n = 4). Data are representative of 3 separate experiments. * P < 0 .01 versus control. ** P < 0 .001 versus control

Techniques Used: Infection, Expressing, shRNA, Quantitative RT-PCR, Lysis, Western Blot, Staining, MTT Assay

Silencing MAFb enhanced PIs-induced apoptosis and activation of caspases. SACHI/sh MAFb or SACHI/shCon cells were treated with serial concentrations of Bzb ( a ) or CFZ ( b and c ) with ( c ) or without HBMSC ( a and b ) for 14 h and apoptotic cell numbers determined by Annexin V staining and flow cytometry analysis as described in supplemental data. Results are presented as mean ± SE ( n = 3). * P < 0 .01 versus control. ** P < 0 .001 versus control. SACHI/sh MAFb and SACHI/shCon cells were treated with indicated concentrations of Bzb (A) or CFZ ( b ) for14 hours. Protein was resolved in SDS-PAGE. The full-length (FL) and cleavage fragments (CFs) of each indicated protein were determined by immunoblotting analysis using antibodies specifically recognizing of caspases-3, − 7, − 8 and − 9, PARP and lamin A/C

Figure Legend Snippet: Silencing MAFb enhanced PIs-induced apoptosis and activation of caspases. SACHI/sh MAFb or SACHI/shCon cells were treated with serial concentrations of Bzb ( a ) or CFZ ( b and c ) with ( c ) or without HBMSC ( a and b ) for 14 h and apoptotic cell numbers determined by Annexin V staining and flow cytometry analysis as described in supplemental data. Results are presented as mean ± SE ( n = 3). * P < 0 .01 versus control. ** P < 0 .001 versus control. SACHI/sh MAFb and SACHI/shCon cells were treated with indicated concentrations of Bzb (A) or CFZ ( b ) for14 hours. Protein was resolved in SDS-PAGE. The full-length (FL) and cleavage fragments (CFs) of each indicated protein were determined by immunoblotting analysis using antibodies specifically recognizing of caspases-3, − 7, − 8 and − 9, PARP and lamin A/C

Techniques Used: Activation Assay, Staining, Flow Cytometry, SDS Page, Western Blot

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gene exp mafb hs00271378 s1 (Thermo Fisher)

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