gene exp sgpl1 mm00486079 m1 (Thermo Fisher)
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gene exp sgpl1 mm00486079 m1
Gene Exp Sgpl1 Mm00486079 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microarray+probe+sets/pmc02856294-65-5-31?v=Thermo+Fisher
Average 89 stars, based on 1 article reviews
Gene Exp Sgpl1 Mm00486079 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microarray+probe+sets/pmc02856294-65-5-31?v=Thermo+Fisher
Average 89 stars, based on 1 article reviews
gene exp sgpl1 mm00486079 m1 - by Bioz Stars,
2026-07
89/100 stars
Images
1) Product Images from "Sphingosine 1-Phosphate Lyase Deficiency Disrupts Lipid Homeostasis in Liver * "
Article Title: Sphingosine 1-Phosphate Lyase Deficiency Disrupts Lipid Homeostasis in Liver
Journal: The Journal of Biological Chemistry
doi: 10.1074/jbc.M109.081489
Figure Legend Snippet: Gene trap disruption of the Sgpl1 gene causes S1P lyase deficiency. A, sphingolipid metabolic pathway and linkage to triacylglycerol and phosphatidylethanolamine synthesis. B, appearance and life span of Sgpl1−/−, Sgpl1+/−, and Sgpl1+/+ mice. C, RT-qPCR of Sgpl1 mRNA using RNA of various tissues from Sgpl1−/− (gray bars) and Sgpl1+/+ (open bars) mice. Gapdh was used as a reference gene for normalization. SI, small intestine. Data represent mean values ± S.E. D, top panel: Western blot of S1P lyase protein in various tissues from Sgpl1−/− and Sgpl1+/+ mice. Bottom panel, same blot probed with antibody to β-actin as a loading control. E, S1P lyase enzyme activity in extracts of various tissues from Sgpl1−/− (gray bars) and Sgpl1+/+ (open bars) mice. Data represent mean values ± S.E. of representative results from three independent experiments.
Techniques Used: Disruption, Quantitative RT-PCR, Western Blot, Control, Activity Assay
Figure Legend Snippet: Sphingolipid levels are elevated in serum of Sgpl1−/− mice. Sphingolipid levels were determined in serum of Sgpl1−/− (gray bars) and Sgpl1+/+ (open bars) mice by LC-MS. A, total ceramide (Cer), sphingosine (Sph). B, total sphingomyelin (SM). C, individual ceramide fatty acid chain species. D, individual sphingomyelin fatty acid chain species. Data represent mean values ± S.E. n = 3. *, p < 0.05; **, p < 0.01; ***, p < 0.005, paired Student's t test.
Techniques Used: Liquid Chromatography with Mass Spectroscopy
Figure Legend Snippet: Sgpl1−/− mice are hyperlipidemic. A, triacylglycerol (TAG), phospholipids, total cholesterol (TC), free cholesterol (FC), and cholesterol esters (CE) were determined in the serum of Sgpl1−/− (gray bars) and Sgpl1+/+ (open bars) mice. Data represent mean values ± S.E. n = 7. **, p < 0.01; ***, p < 0.005, paired Student's t test. B, pooled serum samples from Sgpl1−/− (closed circles) and Sgpl1+/+ (open circles) mice (n = 3 each genotype) were fractionated by FPLC to separate VLDL, LDL, and HDL.
Techniques Used:
Figure Legend Snippet: Sphingolipids are elevated in the liver of Sgpl1−/− mice. Sphingolipid levels were determined in the liver of Sgpl1−/− (gray bars) and Sgpl1+/+ (open bars) mice by LC-MS. A, total ceramide (Cer), sphingosine (Sph). B, total sphingomyelin (SM). C, individual ceramide fatty acid chain species. D, individual sphingomyelin fatty acid chain species. Data represent mean values ± S.E. n = 3. *, p < 0.05; **, p < 0.01; ***, p < 0.005; paired Student's t test.
Techniques Used: Liquid Chromatography with Mass Spectroscopy
Figure Legend Snippet: Lipid profile is altered in the liver of Sgpl1−/− mice. Lipid levels were determined in the liver of Sgpl1−/− (gray bars) and Sgpl1+/+ (open bars) mice as described under “Experimental Procedures.” PE, phosphatidylethanolamine; PC, phosphatidylcholine; PS, phosphatidylserine; LYPC, lysophosphatidylcholine; CL, cardiolipin; FA, fatty acids; DAG, diacylglycerol; TAG, triacylglycerol; CE, cholesterol esters; FC, free cholesterol. Data represent mean values ± S.E. n = 3. *, p < 0.05; **, p < 0.01; ***, p < 0.005; paired Student's t test.
Techniques Used:
Figure Legend Snippet: Excess lipids are stored in the liver of Sgpl1−/− mice. Paraffin-embedded liver sections from (A) Sgpl1+/+ or (B) Sgpl1−/− mice were stained with H&E (×10 magnification). Frozen sections of liver from (C) Sgpl1+/+ or (D) Sgpl1−/− mice were stained with Oil red O (×40 magnification). Arrows indicate lipid deposits. Liver samples from (E) Sgpl1+/+ or (F) Sgpl1−/− mice were analyzed by electron microscopy (×6000 magnification). Arrowheads indicate examples of lipid droplets. Liver sections from (G) Sgpl1+/+ or (H) Sgpl1−/− mice immunostained with LAMP-2 antibody (brown reaction product) and counterstained with hematoxylin (×100 magnification).
Techniques Used: Staining, Electron Microscopy
Figure Legend Snippet: Adiposity is reduced in Sgpl1−/− mice. A, weights of the mice were taken at about 18 days after birth. ***, p < 0.005. Sgpl1−/− (gray bars) and Sgpl1+/+ (open bars) mice were subjected to quantitative magnetic resonance body composition analysis to determine their fat (B) and lean mass (C) content. The fat mass as a percent of body weight is presented in (D). Data represent mean values ± S.E. n = 3. ***, p < 0.005, paired Student's t test.
Techniques Used:
Figure Legend Snippet: Lipid metabolism gene expression profile in the liver of Sgpl1−/− mice exhibits widespread alteration. A, Affymetrix microarray gene expression analysis was performed with liver RNA from three Sgpl1+/+ and three Sgpl1−/− mice. The raw signal values of the genes from the GO category lipid metabolic process were clustered to produce a heat map as described under “Experimental Procedures.” Blue corresponds to reduced expression relative to red, which corresponds to relatively increased expression. RT-qPCR analysis of (B) Ppara and Pparg, (C) Sptlc1, and Sptlc2, (D) Sphk1 and Sphk2, and (E) Sgpp1 and Sgpp2 in liver mRNA from Sgpl1−/− (gray bars) and Sgpl1+/+ (open bars) mice. Data represent mean values ± S.E. n = 3. *, p < 0.05; ***, p < 0.005, paired Student's t test.
Techniques Used: Gene Expression, Microarray, Expressing, Quantitative RT-PCR