Review



mechanical microarray microspotter microgrid ii  (BioRobotics Ltd)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 90
      Buy from Supplier

    Structured Review

    BioRobotics Ltd mechanical microarray microspotter microgrid ii
    Mechanical Microarray Microspotter Microgrid Ii, supplied by BioRobotics Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microarray+printer+biorobotics+microgrid+ii/mechanical+microarray+microspotter+microgrid+ii/pm26312549-163-16-21
    Average 90 stars, based on 1 article reviews
    mechanical microarray microspotter microgrid ii - by Bioz Stars, 2026-10
    90/100 stars

    Images

    Related Articles

    Construct:

    Article Title: AUTEN-67, an autophagy-enhancing drug candidate with potent antiaging and neuroprotective effects.
    Article Snippet: Small molecule microarrays were constructed and prepared for screening as previously described.57,58 Briefly, for printing with MicroGrid II mechanical microarray microspotter (BioRobotics, Cambridge, UK) chemically activated glass slides (Avicor Ltd., Szeged, Hungary) were used, which contain a treelike, branched dendrimer structure with reactive functional groups at the terminal position.59 Fluorescent labeled MTMR14 was prepared with Alexa Fluor 647 D ow nl oa de d by [ U ni ve rs ity o f C am br id ge ] at 0 5: 55 2 9 A ug us t 2 01 5 carboxylic acid succinimidyl ester (Molecular Probes, A-20106) as previously described.60 The affinity experiments were carried out in a hybridization station (Ventana Discovery, Tucson, AZ, USA).

    Microarray:

    Article Title: AUTEN-67, an autophagy-enhancing drug candidate with potent antiaging and neuroprotective effects.
    Article Snippet: Small molecule microarrays were constructed and prepared for screening as previously described.57,58 Briefly, for printing with MicroGrid II mechanical microarray microspotter (BioRobotics, Cambridge, UK) chemically activated glass slides (Avicor Ltd., Szeged, Hungary) were used, which contain a treelike, branched dendrimer structure with reactive functional groups at the terminal position.59 Fluorescent labeled MTMR14 was prepared with Alexa Fluor 647 D ow nl oa de d by [ U ni ve rs ity o f C am br id ge ] at 0 5: 55 2 9 A ug us t 2 01 5 carboxylic acid succinimidyl ester (Molecular Probes, A-20106) as previously described.60 The affinity experiments were carried out in a hybridization station (Ventana Discovery, Tucson, AZ, USA).

    Functional Assay:

    Article Title: AUTEN-67, an autophagy-enhancing drug candidate with potent antiaging and neuroprotective effects.
    Article Snippet: Small molecule microarrays were constructed and prepared for screening as previously described.57,58 Briefly, for printing with MicroGrid II mechanical microarray microspotter (BioRobotics, Cambridge, UK) chemically activated glass slides (Avicor Ltd., Szeged, Hungary) were used, which contain a treelike, branched dendrimer structure with reactive functional groups at the terminal position.59 Fluorescent labeled MTMR14 was prepared with Alexa Fluor 647 D ow nl oa de d by [ U ni ve rs ity o f C am br id ge ] at 0 5: 55 2 9 A ug us t 2 01 5 carboxylic acid succinimidyl ester (Molecular Probes, A-20106) as previously described.60 The affinity experiments were carried out in a hybridization station (Ventana Discovery, Tucson, AZ, USA).

    Labeling:

    Article Title: AUTEN-67, an autophagy-enhancing drug candidate with potent antiaging and neuroprotective effects.
    Article Snippet: Small molecule microarrays were constructed and prepared for screening as previously described.57,58 Briefly, for printing with MicroGrid II mechanical microarray microspotter (BioRobotics, Cambridge, UK) chemically activated glass slides (Avicor Ltd., Szeged, Hungary) were used, which contain a treelike, branched dendrimer structure with reactive functional groups at the terminal position.59 Fluorescent labeled MTMR14 was prepared with Alexa Fluor 647 D ow nl oa de d by [ U ni ve rs ity o f C am br id ge ] at 0 5: 55 2 9 A ug us t 2 01 5 carboxylic acid succinimidyl ester (Molecular Probes, A-20106) as previously described.60 The affinity experiments were carried out in a hybridization station (Ventana Discovery, Tucson, AZ, USA).

    Hybridization:

    Article Title: AUTEN-67, an autophagy-enhancing drug candidate with potent antiaging and neuroprotective effects.
    Article Snippet: Small molecule microarrays were constructed and prepared for screening as previously described.57,58 Briefly, for printing with MicroGrid II mechanical microarray microspotter (BioRobotics, Cambridge, UK) chemically activated glass slides (Avicor Ltd., Szeged, Hungary) were used, which contain a treelike, branched dendrimer structure with reactive functional groups at the terminal position.59 Fluorescent labeled MTMR14 was prepared with Alexa Fluor 647 D ow nl oa de d by [ U ni ve rs ity o f C am br id ge ] at 0 5: 55 2 9 A ug us t 2 01 5 carboxylic acid succinimidyl ester (Molecular Probes, A-20106) as previously described.60 The affinity experiments were carried out in a hybridization station (Ventana Discovery, Tucson, AZ, USA).



    Similar Products

    microarray printer biorobotics microgrid ii  (BioRobotics Ltd)
    90
    BioRobotics Ltd microarray printer biorobotics microgrid ii
    Testing the limits of NRF2 binding. Systematic multivariate ARE results by protein binding <t>microarray</t> for NRF2-MAFG heterodimer are shown. Tolerance for sequence variation was studied for positions 1–3 (white), 6–8 (grey) and 9–11 (dark grey) ( A ) and positions 1, 5 and 11 (seed sequences are shown in grey) ( B ). Results are depicted as measured binding relative to NQO1.ARE binding (black) (mean ± S.E.M., n = 45).
    Microarray Printer Biorobotics Microgrid Ii, supplied by BioRobotics Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microarray+printer+biorobotics+microgrid+ii/microgrid+ii+microarray+robot/pmc04770247-73-24-26
    Average 90 stars, based on 1 article reviews
    microarray printer biorobotics microgrid ii - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Testing the limits of NRF2 binding. Systematic multivariate ARE results by protein binding microarray for NRF2-MAFG heterodimer are shown. Tolerance for sequence variation was studied for positions 1–3 (white), 6–8 (grey) and 9–11 (dark grey) ( A ) and positions 1, 5 and 11 (seed sequences are shown in grey) ( B ). Results are depicted as measured binding relative to NQO1.ARE binding (black) (mean ± S.E.M., n = 45).

    Journal: Nucleic Acids Research

    Article Title: The Effects of Sequence Variation on Genome-wide NRF2 Binding—New Target Genes and Regulatory SNPs

    doi: 10.1093/nar/gkw052

    Figure Lengend Snippet: Testing the limits of NRF2 binding. Systematic multivariate ARE results by protein binding microarray for NRF2-MAFG heterodimer are shown. Tolerance for sequence variation was studied for positions 1–3 (white), 6–8 (grey) and 9–11 (dark grey) ( A ) and positions 1, 5 and 11 (seed sequences are shown in grey) ( B ). Results are depicted as measured binding relative to NQO1.ARE binding (black) (mean ± S.E.M., n = 45).

    Article Snippet: The dilutions were dispensed in a 384-well plate (polypropylene plate No 267462, Nunc, N.Y, USA) and printed onto the avidin-coated glass slides with a microarray printer (BioRobotics MicroGrid II, BioRobotics Ltd, Cambridge, UK).

    Techniques: Binding Assay, Protein Binding, Microarray, Sequencing

    A SNP in FTL promoter has drastic effects of NRF2 binding and transcriptional activation. ( A ) A promoter analysis of the FTL gene at chr19 showing the location of experimentally verified NRF2 binding ARE together with dbSNP (v138) and ENCODE ChIP-seq data. ChIP-seq track displays combined MAFF and MAFK binding signals in H1-hESC (MAFK), K562 (MAFF, MAFK), HeLa-S3 (MAFK), HepG (MAFF, MAFK) and IMR90 (MAFK) cell lines. ( B ) Detailed view showing FTL ARE sequence and the SNP (rs113067944, A→C) position. ( C ) Protein binding microarray results for FTL.ARE.A and the SNP bearing FTL.ARE.C. Results are calculated as measured binding relative to NQO1.ARE binding (mean ± S.E.M, n = 39.). Scramble oligonucleotides served as negative control. ( D ) HEK-293T cells were transfected with NQO1-ARE and FTL-ARE bearing either allele A or allele C with and without NRF2 -expressing plasmids. Twenty-four h after transfection cells were treated with NRF2 inducer (L-SFN) for 16 h followed by luciferase activity measurements. An empty pGL3 promoter vector served as control and activities were normalized to β-galactosidase activity. Results are shown relative to control (mean± S.E.M, n = 4).

    Journal: Nucleic Acids Research

    Article Title: The Effects of Sequence Variation on Genome-wide NRF2 Binding—New Target Genes and Regulatory SNPs

    doi: 10.1093/nar/gkw052

    Figure Lengend Snippet: A SNP in FTL promoter has drastic effects of NRF2 binding and transcriptional activation. ( A ) A promoter analysis of the FTL gene at chr19 showing the location of experimentally verified NRF2 binding ARE together with dbSNP (v138) and ENCODE ChIP-seq data. ChIP-seq track displays combined MAFF and MAFK binding signals in H1-hESC (MAFK), K562 (MAFF, MAFK), HeLa-S3 (MAFK), HepG (MAFF, MAFK) and IMR90 (MAFK) cell lines. ( B ) Detailed view showing FTL ARE sequence and the SNP (rs113067944, A→C) position. ( C ) Protein binding microarray results for FTL.ARE.A and the SNP bearing FTL.ARE.C. Results are calculated as measured binding relative to NQO1.ARE binding (mean ± S.E.M, n = 39.). Scramble oligonucleotides served as negative control. ( D ) HEK-293T cells were transfected with NQO1-ARE and FTL-ARE bearing either allele A or allele C with and without NRF2 -expressing plasmids. Twenty-four h after transfection cells were treated with NRF2 inducer (L-SFN) for 16 h followed by luciferase activity measurements. An empty pGL3 promoter vector served as control and activities were normalized to β-galactosidase activity. Results are shown relative to control (mean± S.E.M, n = 4).

    Article Snippet: The dilutions were dispensed in a 384-well plate (polypropylene plate No 267462, Nunc, N.Y, USA) and printed onto the avidin-coated glass slides with a microarray printer (BioRobotics MicroGrid II, BioRobotics Ltd, Cambridge, UK).

    Techniques: Binding Assay, Activation Assay, ChIP-sequencing, Sequencing, Protein Binding, Microarray, Negative Control, Transfection, Expressing, Luciferase, Activity Assay, Plasmid Preparation, Control