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NanoView Biosciences single-ev microarray imaging
Evaluation of the immune marker expression from light promoted DEVs using NanoView single‐EV <t>microarray.</t> (a) Schematic diagram of sensitive multimarker detection from EVs captured on microarray chip spot. (b) Representative fluorescent microarray imaging of CD63 + (red), MHC‐I + (green), CD86 + (blue) DEVs on CD9 (upper) and CD81 (lower) capture spots. Bottom image frames are enlarged views of captured EVs under each fluorescent channel as well as the overlay. (c) Particle counts of captured CD63 + , MHC‐I + , and CD86 + DEVs from different EV sample conditions on both CD81 and CD9 capture spots ( n = 3). (d) Particle counts of captured CD63 + , MHC‐II + , and CD86 + EVs from different EV sample conditions on both CD81 and CD9 capture spots ( n = 3). (e) Radar chart analysis of the relative coexpression levels of multimarkers in various combinations from different EV sample conditions on both CD81 and CD9 capture spots (f)
Single Ev Microarray Imaging, supplied by NanoView Biosciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microarray+images/single+ev+microarray+imaging/pmc08886920-185-14-19
Average 90 stars, based on 1 article reviews
single-ev microarray imaging - by Bioz Stars, 2026-09
90/100 stars

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1) Product Images from "Light‐induced high‐efficient cellular production of immune functional extracellular vesicles"

Article Title: Light‐induced high‐efficient cellular production of immune functional extracellular vesicles

Journal: Journal of Extracellular Vesicles

doi: 10.1002/jev2.12194

Evaluation of the immune marker expression from light promoted DEVs using NanoView single‐EV microarray. (a) Schematic diagram of sensitive multimarker detection from EVs captured on microarray chip spot. (b) Representative fluorescent microarray imaging of CD63 + (red), MHC‐I + (green), CD86 + (blue) DEVs on CD9 (upper) and CD81 (lower) capture spots. Bottom image frames are enlarged views of captured EVs under each fluorescent channel as well as the overlay. (c) Particle counts of captured CD63 + , MHC‐I + , and CD86 + DEVs from different EV sample conditions on both CD81 and CD9 capture spots ( n = 3). (d) Particle counts of captured CD63 + , MHC‐II + , and CD86 + EVs from different EV sample conditions on both CD81 and CD9 capture spots ( n = 3). (e) Radar chart analysis of the relative coexpression levels of multimarkers in various combinations from different EV sample conditions on both CD81 and CD9 capture spots (f)
Figure Legend Snippet: Evaluation of the immune marker expression from light promoted DEVs using NanoView single‐EV microarray. (a) Schematic diagram of sensitive multimarker detection from EVs captured on microarray chip spot. (b) Representative fluorescent microarray imaging of CD63 + (red), MHC‐I + (green), CD86 + (blue) DEVs on CD9 (upper) and CD81 (lower) capture spots. Bottom image frames are enlarged views of captured EVs under each fluorescent channel as well as the overlay. (c) Particle counts of captured CD63 + , MHC‐I + , and CD86 + DEVs from different EV sample conditions on both CD81 and CD9 capture spots ( n = 3). (d) Particle counts of captured CD63 + , MHC‐II + , and CD86 + EVs from different EV sample conditions on both CD81 and CD9 capture spots ( n = 3). (e) Radar chart analysis of the relative coexpression levels of multimarkers in various combinations from different EV sample conditions on both CD81 and CD9 capture spots (f)

Techniques Used: Marker, Expressing, Microarray, Imaging

Related Articles

Clarification Assay:

Article Title: Development of surface engineered antigenic exosomes as vaccines for respiratory syncytial virus
Article Snippet: We also used the single-EV microarray imaging technology from NanoView to directly determine the MHC-I expression level from prepared exosomes as well as their functional markers shown in Fig. F. The specific antibody capture on each NanoView chip spot allows the affinity capture of exosomes based on their surface markers for further multiplexed affinity probing.

Article Title: Development of surface engineered antigenic exosomes as vaccines for respiratory syncytial virus.
Article Snippet: We also used the single-EV microarray imaging technology from NanoView to directly determine the MHC-I expression level from prepared exosomes as well as their functional markers shown in Fig. 1F.

Article Title: Light-induced high-efficient cellular production of immune functional extracellular vesicles.
Article Snippet: Therefore, for further clarification, we investigated immune marker expression levels from secreted EVs in the next step. . Assess the immune marker expression from light promoted DEVs In order to further characterize the immune activity of light-promotedDEVs, we employed a single-EVmicroarray imaging technology from NanoView to directly determine whether light promoted DEVs are significantly immune potent with expression of functional markers.

Article Title: Light‐induced high‐efficient cellular production of immune functional extracellular vesicles
Article Snippet: In order to further characterize the immune activity of light‐promoted DEVs, we employed a single‐EV microarray imaging technology from NanoView to directly determine whether light promoted DEVs are significantly immune potent with expression of functional markers.

Marker:

Article Title: Development of surface engineered antigenic exosomes as vaccines for respiratory syncytial virus
Article Snippet: We also used the single-EV microarray imaging technology from NanoView to directly determine the MHC-I expression level from prepared exosomes as well as their functional markers shown in Fig. F. The specific antibody capture on each NanoView chip spot allows the affinity capture of exosomes based on their surface markers for further multiplexed affinity probing.

Article Title: Development of surface engineered antigenic exosomes as vaccines for respiratory syncytial virus.
Article Snippet: We also used the single-EV microarray imaging technology from NanoView to directly determine the MHC-I expression level from prepared exosomes as well as their functional markers shown in Fig. 1F.

Article Title: Light-induced high-efficient cellular production of immune functional extracellular vesicles.
Article Snippet: Therefore, for further clarification, we investigated immune marker expression levels from secreted EVs in the next step. . Assess the immune marker expression from light promoted DEVs In order to further characterize the immune activity of light-promotedDEVs, we employed a single-EVmicroarray imaging technology from NanoView to directly determine whether light promoted DEVs are significantly immune potent with expression of functional markers.

Article Title: Light‐induced high‐efficient cellular production of immune functional extracellular vesicles
Article Snippet: In order to further characterize the immune activity of light‐promoted DEVs, we employed a single‐EV microarray imaging technology from NanoView to directly determine whether light promoted DEVs are significantly immune potent with expression of functional markers.

Expressing:

Article Title: Development of surface engineered antigenic exosomes as vaccines for respiratory syncytial virus
Article Snippet: We also used the single-EV microarray imaging technology from NanoView to directly determine the MHC-I expression level from prepared exosomes as well as their functional markers shown in Fig. F. The specific antibody capture on each NanoView chip spot allows the affinity capture of exosomes based on their surface markers for further multiplexed affinity probing.

Article Title: Development of surface engineered antigenic exosomes as vaccines for respiratory syncytial virus.
Article Snippet: We also used the single-EV microarray imaging technology from NanoView to directly determine the MHC-I expression level from prepared exosomes as well as their functional markers shown in Fig. 1F.

Article Title: Light-induced high-efficient cellular production of immune functional extracellular vesicles.
Article Snippet: Therefore, for further clarification, we investigated immune marker expression levels from secreted EVs in the next step. . Assess the immune marker expression from light promoted DEVs In order to further characterize the immune activity of light-promotedDEVs, we employed a single-EVmicroarray imaging technology from NanoView to directly determine whether light promoted DEVs are significantly immune potent with expression of functional markers.

Article Title: Light‐induced high‐efficient cellular production of immune functional extracellular vesicles
Article Snippet: In order to further characterize the immune activity of light‐promoted DEVs, we employed a single‐EV microarray imaging technology from NanoView to directly determine whether light promoted DEVs are significantly immune potent with expression of functional markers.

Activity Assay:

Article Title: Development of surface engineered antigenic exosomes as vaccines for respiratory syncytial virus
Article Snippet: We also used the single-EV microarray imaging technology from NanoView to directly determine the MHC-I expression level from prepared exosomes as well as their functional markers shown in Fig. F. The specific antibody capture on each NanoView chip spot allows the affinity capture of exosomes based on their surface markers for further multiplexed affinity probing.

Article Title: Development of surface engineered antigenic exosomes as vaccines for respiratory syncytial virus.
Article Snippet: We also used the single-EV microarray imaging technology from NanoView to directly determine the MHC-I expression level from prepared exosomes as well as their functional markers shown in Fig. 1F.

Article Title: Light-induced high-efficient cellular production of immune functional extracellular vesicles.
Article Snippet: Therefore, for further clarification, we investigated immune marker expression levels from secreted EVs in the next step. . Assess the immune marker expression from light promoted DEVs In order to further characterize the immune activity of light-promotedDEVs, we employed a single-EVmicroarray imaging technology from NanoView to directly determine whether light promoted DEVs are significantly immune potent with expression of functional markers.

Article Title: Light‐induced high‐efficient cellular production of immune functional extracellular vesicles
Article Snippet: In order to further characterize the immune activity of light‐promoted DEVs, we employed a single‐EV microarray imaging technology from NanoView to directly determine whether light promoted DEVs are significantly immune potent with expression of functional markers.

Imaging:

Article Title: Development of surface engineered antigenic exosomes as vaccines for respiratory syncytial virus
Article Snippet: We also used the single-EV microarray imaging technology from NanoView to directly determine the MHC-I expression level from prepared exosomes as well as their functional markers shown in Fig. F. The specific antibody capture on each NanoView chip spot allows the affinity capture of exosomes based on their surface markers for further multiplexed affinity probing.

Article Title: Development of surface engineered antigenic exosomes as vaccines for respiratory syncytial virus.
Article Snippet: We also used the single-EV microarray imaging technology from NanoView to directly determine the MHC-I expression level from prepared exosomes as well as their functional markers shown in Fig. 1F.

Article Title: Light-induced high-efficient cellular production of immune functional extracellular vesicles.
Article Snippet: Therefore, for further clarification, we investigated immune marker expression levels from secreted EVs in the next step. . Assess the immune marker expression from light promoted DEVs In order to further characterize the immune activity of light-promotedDEVs, we employed a single-EVmicroarray imaging technology from NanoView to directly determine whether light promoted DEVs are significantly immune potent with expression of functional markers.

Article Title: Light‐induced high‐efficient cellular production of immune functional extracellular vesicles
Article Snippet: In order to further characterize the immune activity of light‐promoted DEVs, we employed a single‐EV microarray imaging technology from NanoView to directly determine whether light promoted DEVs are significantly immune potent with expression of functional markers.

Functional Assay:

Article Title: Development of surface engineered antigenic exosomes as vaccines for respiratory syncytial virus
Article Snippet: We also used the single-EV microarray imaging technology from NanoView to directly determine the MHC-I expression level from prepared exosomes as well as their functional markers shown in Fig. F. The specific antibody capture on each NanoView chip spot allows the affinity capture of exosomes based on their surface markers for further multiplexed affinity probing.

Article Title: Development of surface engineered antigenic exosomes as vaccines for respiratory syncytial virus.
Article Snippet: We also used the single-EV microarray imaging technology from NanoView to directly determine the MHC-I expression level from prepared exosomes as well as their functional markers shown in Fig. 1F.

Article Title: Light-induced high-efficient cellular production of immune functional extracellular vesicles.
Article Snippet: Therefore, for further clarification, we investigated immune marker expression levels from secreted EVs in the next step. . Assess the immune marker expression from light promoted DEVs In order to further characterize the immune activity of light-promotedDEVs, we employed a single-EVmicroarray imaging technology from NanoView to directly determine whether light promoted DEVs are significantly immune potent with expression of functional markers.

Article Title: Light‐induced high‐efficient cellular production of immune functional extracellular vesicles
Article Snippet: In order to further characterize the immune activity of light‐promoted DEVs, we employed a single‐EV microarray imaging technology from NanoView to directly determine whether light promoted DEVs are significantly immune potent with expression of functional markers.

Microarray:

Article Title: Development of surface engineered antigenic exosomes as vaccines for respiratory syncytial virus
Article Snippet: We also used the single-EV microarray imaging technology from NanoView to directly determine the MHC-I expression level from prepared exosomes as well as their functional markers shown in Fig. F. The specific antibody capture on each NanoView chip spot allows the affinity capture of exosomes based on their surface markers for further multiplexed affinity probing.

Article Title: Development of surface engineered antigenic exosomes as vaccines for respiratory syncytial virus.
Article Snippet: We also used the single-EV microarray imaging technology from NanoView to directly determine the MHC-I expression level from prepared exosomes as well as their functional markers shown in Fig. 1F.

Article Title: Light-induced high-efficient cellular production of immune functional extracellular vesicles.
Article Snippet: Therefore, for further clarification, we investigated immune marker expression levels from secreted EVs in the next step. . Assess the immune marker expression from light promoted DEVs In order to further characterize the immune activity of light-promotedDEVs, we employed a single-EVmicroarray imaging technology from NanoView to directly determine whether light promoted DEVs are significantly immune potent with expression of functional markers.

Article Title: Light‐induced high‐efficient cellular production of immune functional extracellular vesicles
Article Snippet: In order to further characterize the immune activity of light‐promoted DEVs, we employed a single‐EV microarray imaging technology from NanoView to directly determine whether light promoted DEVs are significantly immune potent with expression of functional markers.

Immunopeptidomics:

Article Title: Development of surface engineered antigenic exosomes as vaccines for respiratory syncytial virus
Article Snippet: We also used the single-EV microarray imaging technology from NanoView to directly determine the MHC-I expression level from prepared exosomes as well as their functional markers shown in Fig. F. The specific antibody capture on each NanoView chip spot allows the affinity capture of exosomes based on their surface markers for further multiplexed affinity probing.

Article Title: Development of surface engineered antigenic exosomes as vaccines for respiratory syncytial virus.
Article Snippet: We also used the single-EV microarray imaging technology from NanoView to directly determine the MHC-I expression level from prepared exosomes as well as their functional markers shown in Fig. 1F.

Article Title: Light-induced high-efficient cellular production of immune functional extracellular vesicles.
Article Snippet: Therefore, for further clarification, we investigated immune marker expression levels from secreted EVs in the next step. . Assess the immune marker expression from light promoted DEVs In order to further characterize the immune activity of light-promotedDEVs, we employed a single-EVmicroarray imaging technology from NanoView to directly determine whether light promoted DEVs are significantly immune potent with expression of functional markers.

Article Title: Light‐induced high‐efficient cellular production of immune functional extracellular vesicles
Article Snippet: In order to further characterize the immune activity of light‐promoted DEVs, we employed a single‐EV microarray imaging technology from NanoView to directly determine whether light promoted DEVs are significantly immune potent with expression of functional markers.

Binding Assay:

Article Title: Development of surface engineered antigenic exosomes as vaccines for respiratory syncytial virus
Article Snippet: We also used the single-EV microarray imaging technology from NanoView to directly determine the MHC-I expression level from prepared exosomes as well as their functional markers shown in Fig. F. The specific antibody capture on each NanoView chip spot allows the affinity capture of exosomes based on their surface markers for further multiplexed affinity probing.

Article Title: Development of surface engineered antigenic exosomes as vaccines for respiratory syncytial virus.
Article Snippet: We also used the single-EV microarray imaging technology from NanoView to directly determine the MHC-I expression level from prepared exosomes as well as their functional markers shown in Fig. 1F.

Article Title: Light-induced high-efficient cellular production of immune functional extracellular vesicles.
Article Snippet: Therefore, for further clarification, we investigated immune marker expression levels from secreted EVs in the next step. . Assess the immune marker expression from light promoted DEVs In order to further characterize the immune activity of light-promotedDEVs, we employed a single-EVmicroarray imaging technology from NanoView to directly determine whether light promoted DEVs are significantly immune potent with expression of functional markers.

Article Title: Light‐induced high‐efficient cellular production of immune functional extracellular vesicles
Article Snippet: In order to further characterize the immune activity of light‐promoted DEVs, we employed a single‐EV microarray imaging technology from NanoView to directly determine whether light promoted DEVs are significantly immune potent with expression of functional markers.

Isolation:

Article Title: Development of surface engineered antigenic exosomes as vaccines for respiratory syncytial virus
Article Snippet: We also used the single-EV microarray imaging technology from NanoView to directly determine the MHC-I expression level from prepared exosomes as well as their functional markers shown in Fig. F. The specific antibody capture on each NanoView chip spot allows the affinity capture of exosomes based on their surface markers for further multiplexed affinity probing.

Article Title: Development of surface engineered antigenic exosomes as vaccines for respiratory syncytial virus.
Article Snippet: We also used the single-EV microarray imaging technology from NanoView to directly determine the MHC-I expression level from prepared exosomes as well as their functional markers shown in Fig. 1F.

Article Title: Light-induced high-efficient cellular production of immune functional extracellular vesicles.
Article Snippet: Therefore, for further clarification, we investigated immune marker expression levels from secreted EVs in the next step. . Assess the immune marker expression from light promoted DEVs In order to further characterize the immune activity of light-promotedDEVs, we employed a single-EVmicroarray imaging technology from NanoView to directly determine whether light promoted DEVs are significantly immune potent with expression of functional markers.

Article Title: Light‐induced high‐efficient cellular production of immune functional extracellular vesicles
Article Snippet: In order to further characterize the immune activity of light‐promoted DEVs, we employed a single‐EV microarray imaging technology from NanoView to directly determine whether light promoted DEVs are significantly immune potent with expression of functional markers.

Zeta Potential Analyzer:

Article Title: Development of surface engineered antigenic exosomes as vaccines for respiratory syncytial virus
Article Snippet: We also used the single-EV microarray imaging technology from NanoView to directly determine the MHC-I expression level from prepared exosomes as well as their functional markers shown in Fig. F. The specific antibody capture on each NanoView chip spot allows the affinity capture of exosomes based on their surface markers for further multiplexed affinity probing.

Article Title: Development of surface engineered antigenic exosomes as vaccines for respiratory syncytial virus.
Article Snippet: We also used the single-EV microarray imaging technology from NanoView to directly determine the MHC-I expression level from prepared exosomes as well as their functional markers shown in Fig. 1F.

Article Title: Light-induced high-efficient cellular production of immune functional extracellular vesicles.
Article Snippet: Therefore, for further clarification, we investigated immune marker expression levels from secreted EVs in the next step. . Assess the immune marker expression from light promoted DEVs In order to further characterize the immune activity of light-promotedDEVs, we employed a single-EVmicroarray imaging technology from NanoView to directly determine whether light promoted DEVs are significantly immune potent with expression of functional markers.

Article Title: Light‐induced high‐efficient cellular production of immune functional extracellular vesicles
Article Snippet: In order to further characterize the immune activity of light‐promoted DEVs, we employed a single‐EV microarray imaging technology from NanoView to directly determine whether light promoted DEVs are significantly immune potent with expression of functional markers.

Control:

Article Title: Development of surface engineered antigenic exosomes as vaccines for respiratory syncytial virus
Article Snippet: We also used the single-EV microarray imaging technology from NanoView to directly determine the MHC-I expression level from prepared exosomes as well as their functional markers shown in Fig. F. The specific antibody capture on each NanoView chip spot allows the affinity capture of exosomes based on their surface markers for further multiplexed affinity probing.

Article Title: Development of surface engineered antigenic exosomes as vaccines for respiratory syncytial virus.
Article Snippet: We also used the single-EV microarray imaging technology from NanoView to directly determine the MHC-I expression level from prepared exosomes as well as their functional markers shown in Fig. 1F.

Article Title: Light-induced high-efficient cellular production of immune functional extracellular vesicles.
Article Snippet: Therefore, for further clarification, we investigated immune marker expression levels from secreted EVs in the next step. . Assess the immune marker expression from light promoted DEVs In order to further characterize the immune activity of light-promotedDEVs, we employed a single-EVmicroarray imaging technology from NanoView to directly determine whether light promoted DEVs are significantly immune potent with expression of functional markers.

Article Title: Light‐induced high‐efficient cellular production of immune functional extracellular vesicles
Article Snippet: In order to further characterize the immune activity of light‐promoted DEVs, we employed a single‐EV microarray imaging technology from NanoView to directly determine whether light promoted DEVs are significantly immune potent with expression of functional markers.

Flow Cytometry:

Article Title: Development of surface engineered antigenic exosomes as vaccines for respiratory syncytial virus
Article Snippet: We also used the single-EV microarray imaging technology from NanoView to directly determine the MHC-I expression level from prepared exosomes as well as their functional markers shown in Fig. F. The specific antibody capture on each NanoView chip spot allows the affinity capture of exosomes based on their surface markers for further multiplexed affinity probing.

Article Title: Development of surface engineered antigenic exosomes as vaccines for respiratory syncytial virus.
Article Snippet: We also used the single-EV microarray imaging technology from NanoView to directly determine the MHC-I expression level from prepared exosomes as well as their functional markers shown in Fig. 1F.

Article Title: Light-induced high-efficient cellular production of immune functional extracellular vesicles.
Article Snippet: Therefore, for further clarification, we investigated immune marker expression levels from secreted EVs in the next step. . Assess the immune marker expression from light promoted DEVs In order to further characterize the immune activity of light-promotedDEVs, we employed a single-EVmicroarray imaging technology from NanoView to directly determine whether light promoted DEVs are significantly immune potent with expression of functional markers.

Article Title: Light‐induced high‐efficient cellular production of immune functional extracellular vesicles
Article Snippet: In order to further characterize the immune activity of light‐promoted DEVs, we employed a single‐EV microarray imaging technology from NanoView to directly determine whether light promoted DEVs are significantly immune potent with expression of functional markers.



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