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CapitalBio Corporation microarray imaging
RT, ST, RI, SI, and YL represent the rhizome tip, shoot tip, rhizome internodes, shoot internodes, and young leaves, respectively. Expression levels were calculated based on the expression level of YL genes set to 1. Expression profiles obtained by real time PCR for one gene, Sb01g036550, were not consistent with data obtained from <t>microarray</t> analysis. Correlation coefficients (r) for the remaining 11 genes were 0.74, 0.74, 0.74, 0.74, 0.77, 0.74, 0.82, 0.76, 0.76, 0.94, and 0.76, from left to right, respectively. Bars donate standard deviation.
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1) Product Images from "Tissue-Specific Transcriptomic Profiling of Sorghum propinquum using a Rice Genome Array"

Article Title: Tissue-Specific Transcriptomic Profiling of Sorghum propinquum using a Rice Genome Array

Journal: PLoS ONE

doi: 10.1371/journal.pone.0060202

RT, ST, RI, SI, and YL represent the rhizome tip, shoot tip, rhizome internodes, shoot internodes, and young leaves, respectively. Expression levels were calculated based on the expression level of YL genes set to 1. Expression profiles obtained by real time PCR for one gene, Sb01g036550, were not consistent with data obtained from microarray analysis. Correlation coefficients (r) for the remaining 11 genes were 0.74, 0.74, 0.74, 0.74, 0.77, 0.74, 0.82, 0.76, 0.76, 0.94, and 0.76, from left to right, respectively. Bars donate standard deviation.
Figure Legend Snippet: RT, ST, RI, SI, and YL represent the rhizome tip, shoot tip, rhizome internodes, shoot internodes, and young leaves, respectively. Expression levels were calculated based on the expression level of YL genes set to 1. Expression profiles obtained by real time PCR for one gene, Sb01g036550, were not consistent with data obtained from microarray analysis. Correlation coefficients (r) for the remaining 11 genes were 0.74, 0.74, 0.74, 0.74, 0.77, 0.74, 0.82, 0.76, 0.76, 0.94, and 0.76, from left to right, respectively. Bars donate standard deviation.

Techniques Used: Expressing, Real-time Polymerase Chain Reaction, Microarray, Standard Deviation

In situ localization of transcripts corresponding to the genes (a) Sb01g047010 and (c) Sb06g028820 in S. propinquum rhizome tips are illustrated; (b) and (d) represent the sense probe for control. Corresponding microarray-based expression profiles of these two genes are also shown as bar graphs for comparison.
Figure Legend Snippet: In situ localization of transcripts corresponding to the genes (a) Sb01g047010 and (c) Sb06g028820 in S. propinquum rhizome tips are illustrated; (b) and (d) represent the sense probe for control. Corresponding microarray-based expression profiles of these two genes are also shown as bar graphs for comparison.

Techniques Used: In Situ, Control, Microarray, Expressing, Comparison

The heat map was produced by analyzing genes comprising rhizome related DEGs for methyl jasmonate (MJ), ethylene (C 2 H 4 ), abscisic acid (ABA), auxin (AUX), gibberellic acid (GA), zeatin, brassinosteroids (BR), and salicylic acid (SA). Subfigures a–g represent the seven gene sets analyzed: DEGs of RT vs. ST in O. longistaminata from (a) transcriptome sequencing and (c) microarray analysis, and (e) results of the present study in S. propinquum ; DEGs of underground tissues (RT and RI) vs. above-ground tissues (ST, SI, and YL) in O. longistaminata from (b) transcriptome sequencing and (d) microarray analysis, and (f) results of the present study in S. propinquum ; and (g) candidate rhizome-enriched genes in S. halepense (pSH) and S. propinquum . In the HORMONOMETER analysis, orange (1) = complete correlation, white (0) = no correlation, and blue (-1) = anti-correlation.
Figure Legend Snippet: The heat map was produced by analyzing genes comprising rhizome related DEGs for methyl jasmonate (MJ), ethylene (C 2 H 4 ), abscisic acid (ABA), auxin (AUX), gibberellic acid (GA), zeatin, brassinosteroids (BR), and salicylic acid (SA). Subfigures a–g represent the seven gene sets analyzed: DEGs of RT vs. ST in O. longistaminata from (a) transcriptome sequencing and (c) microarray analysis, and (e) results of the present study in S. propinquum ; DEGs of underground tissues (RT and RI) vs. above-ground tissues (ST, SI, and YL) in O. longistaminata from (b) transcriptome sequencing and (d) microarray analysis, and (f) results of the present study in S. propinquum ; and (g) candidate rhizome-enriched genes in S. halepense (pSH) and S. propinquum . In the HORMONOMETER analysis, orange (1) = complete correlation, white (0) = no correlation, and blue (-1) = anti-correlation.

Techniques Used: Produced, Sequencing, Microarray

Related Articles

RNA Amplification:

Article Title: Tissue-Specific Transcriptomic Profiling of Sorghum propinquum using a Rice Genome Array
Article Snippet: .. RNA amplification, labeling, and hybridization, and microarray imaging were carried out by a specialized biotech company, CapitalBio Corporation (Beijing, China). ..

Labeling:

Article Title: Tissue-Specific Transcriptomic Profiling of Sorghum propinquum using a Rice Genome Array
Article Snippet: .. RNA amplification, labeling, and hybridization, and microarray imaging were carried out by a specialized biotech company, CapitalBio Corporation (Beijing, China). ..

Hybridization:

Article Title: Tissue-Specific Transcriptomic Profiling of Sorghum propinquum using a Rice Genome Array
Article Snippet: .. RNA amplification, labeling, and hybridization, and microarray imaging were carried out by a specialized biotech company, CapitalBio Corporation (Beijing, China). ..

Microarray:

Article Title: Tissue-Specific Transcriptomic Profiling of Sorghum propinquum using a Rice Genome Array
Article Snippet: .. RNA amplification, labeling, and hybridization, and microarray imaging were carried out by a specialized biotech company, CapitalBio Corporation (Beijing, China). ..

Imaging:

Article Title: Tissue-Specific Transcriptomic Profiling of Sorghum propinquum using a Rice Genome Array
Article Snippet: .. RNA amplification, labeling, and hybridization, and microarray imaging were carried out by a specialized biotech company, CapitalBio Corporation (Beijing, China). ..



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