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one-color microarray hybridization protocol, v6.5  (Agilent technologies)


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    Agilent technologies one-color microarray hybridization protocol, v6.5
    One Color Microarray Hybridization Protocol, V6.5, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microarray+hybridizations+protocol/pm24704562-89-23-24
    Average 90 stars, based on 1 article reviews
    one-color microarray hybridization protocol, v6.5 - by Bioz Stars, 2026-09
    90/100 stars

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    Article Title: Integrated approach to explore the mechanisms of aromatase inhibition and recovery in fathead minnows (Pimephales promelas).
    Article Snippet: Aromatase, a member of the cytochrome P450 superfamily, is a key enzyme in estradiol synthesis that catalyzes the aromatization of androgens into estrogens in ovaries.. Here, we used an integrated approach to assess the mechanistic basis of the direct effects of aromatase inhibition, as well as adaptation and recovery processes in fish.. We exposed female fathead minnows (Pimephales promelas) via the water to 30 lg/L of a model aromatase inhibitor, fadrozole, during 8 days (exposure phase).

    Amplification:

    Article Title: Whole Blood Transcriptome Analysis of Mycoplasma mycoides Subsp. mycoides -Infected Cattle Confirms Immunosuppression but Does Not Reflect Local Inflammation
    Article Snippet: .. To produce Cy3-labeled cRNA, each total RNA sample was amplified and labeled using the Agilent Low Input Quick Amplification Labeling Kit, v6.5, (available online: http://www.chem.agilent.com/library/usermanuals/ ) strictly following the manufacturer’s instructions. ..

    Labeling:

    Article Title: Whole Blood Transcriptome Analysis of Mycoplasma mycoides Subsp. mycoides -Infected Cattle Confirms Immunosuppression but Does Not Reflect Local Inflammation
    Article Snippet: .. To produce Cy3-labeled cRNA, each total RNA sample was amplified and labeled using the Agilent Low Input Quick Amplification Labeling Kit, v6.5, (available online: http://www.chem.agilent.com/library/usermanuals/ ) strictly following the manufacturer’s instructions. ..



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    Image Search Results


    (A) Control and IFIT1-depleted THP1 cells were stimulated with 100 ng/mL LPS for 1, 2, 4, 6, and 24 hr, and transcriptional responses were measured by microarray. The upper heatmap shows genes with substantial expression increases in WT cells compared to their expression level in IFIT1-depleted cells. The lower heatmap shows the sum of the expression difference between WT and IFIT1-depleted cells across the time course . (B and C) The network connections of genes that showed (B) enhanced expression (group 1; ) or (C) reduced expression (group 2; ) in IFIT1-depleted cells, as determined by IPA. (D) Transfection of siRNA into IFIT1 shRNA-expressing cells sustains a stable suppression of IFIT1 induction in response to 100 ng/mL LPS. (E and F) Control and IFIT1-depleted THP1 cells (E) or human primary macrophages (F) were stimulated with 100 ng/mL LPS, and secreted IFN-β protein was measured by ELISA. Data are representative of two (A) or three (D–F) independent experiments. Data in (D)–(F) are expressed as mean SD; *p < 0.05, **p < 0.01 (two-way ANOVA followed by Sidak’s multiple comparison test). See also and , , and .

    Journal: Cell reports

    Article Title: IFIT1 Exerts Opposing Regulatory Effects on the Inflammatory and Interferon Gene Programs in LPS-Activated Human Macrophages

    doi: 10.1016/j.celrep.2018.09.002

    Figure Lengend Snippet: (A) Control and IFIT1-depleted THP1 cells were stimulated with 100 ng/mL LPS for 1, 2, 4, 6, and 24 hr, and transcriptional responses were measured by microarray. The upper heatmap shows genes with substantial expression increases in WT cells compared to their expression level in IFIT1-depleted cells. The lower heatmap shows the sum of the expression difference between WT and IFIT1-depleted cells across the time course . (B and C) The network connections of genes that showed (B) enhanced expression (group 1; ) or (C) reduced expression (group 2; ) in IFIT1-depleted cells, as determined by IPA. (D) Transfection of siRNA into IFIT1 shRNA-expressing cells sustains a stable suppression of IFIT1 induction in response to 100 ng/mL LPS. (E and F) Control and IFIT1-depleted THP1 cells (E) or human primary macrophages (F) were stimulated with 100 ng/mL LPS, and secreted IFN-β protein was measured by ELISA. Data are representative of two (A) or three (D–F) independent experiments. Data in (D)–(F) are expressed as mean SD; *p < 0.05, **p < 0.01 (two-way ANOVA followed by Sidak’s multiple comparison test). See also and , , and .

    Article Snippet: Each condition was represented by two biological replicates. cRNA amplification and labeling were performed using the Illumina TotalPrep RNA Amplification Kit (Ambion), microarray hybridization and scanning protocols followed standard Illumina protocols.

    Techniques: Microarray, Expressing, Transfection, shRNA, Enzyme-linked Immunosorbent Assay

    KEY RESOURCES TABLE

    Journal: Cell reports

    Article Title: IFIT1 Exerts Opposing Regulatory Effects on the Inflammatory and Interferon Gene Programs in LPS-Activated Human Macrophages

    doi: 10.1016/j.celrep.2018.09.002

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: Each condition was represented by two biological replicates. cRNA amplification and labeling were performed using the Illumina TotalPrep RNA Amplification Kit (Ambion), microarray hybridization and scanning protocols followed standard Illumina protocols.

    Techniques: Recombinant, Microarray, shRNA, Software