Review



microarray hybridization and analysis  (Cogenics Inc)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 90
      Buy from Supplier

    Structured Review

    Cogenics Inc microarray hybridization and analysis
    The allograft T Cell <t>microarray</t> data were analyzed by PCA using Partek Genomics Suite. 50% of the variation in samples was revealed in the first two principal components. The first principal component is represented by the X axis and the second principal component by the Y axis. Treatment type appeared to be major source of variation and is indicated by different colored ellipsoids. Green, untreated; Red, CsA only; and Blue, CsA plus peptide. The numbers next to each ellipsoid indicate the number of days post transplantation regardless of treatment type. The type of treatment appeared to be the most significant effect and the treatment time was another significant variable. Each treatment could be seen as distinct groups. Both the untreated (Green) and the cyclosporine treated (Red) samples seemed to form similar cluster shapes. Cyclosporine plus peptide (Blue) sample, on the other hand, was much more spread out. This could be due to another variable such as time after transplantation. Indeed, treatment time did appear to have some effect on the PCA since the ovoids representing 7 day-treatment samples were always to the left of the 1 or 3 days treatments.
    Microarray Hybridization And Analysis, supplied by Cogenics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microarray+hybridization+and+statistical+analysis/microarray+hybridization+and+analysis/pmc02780702-203-1-8
    Average 90 stars, based on 1 article reviews
    microarray hybridization and analysis - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Down Regulation of Genes Involved in T Cell Polarity and Motility during the Induction of Heart Allograft Tolerance by Allochimeric MHC I"

    Article Title: Down Regulation of Genes Involved in T Cell Polarity and Motility during the Induction of Heart Allograft Tolerance by Allochimeric MHC I

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0008020

    The allograft T Cell microarray data were analyzed by PCA using Partek Genomics Suite. 50% of the variation in samples was revealed in the first two principal components. The first principal component is represented by the X axis and the second principal component by the Y axis. Treatment type appeared to be major source of variation and is indicated by different colored ellipsoids. Green, untreated; Red, CsA only; and Blue, CsA plus peptide. The numbers next to each ellipsoid indicate the number of days post transplantation regardless of treatment type. The type of treatment appeared to be the most significant effect and the treatment time was another significant variable. Each treatment could be seen as distinct groups. Both the untreated (Green) and the cyclosporine treated (Red) samples seemed to form similar cluster shapes. Cyclosporine plus peptide (Blue) sample, on the other hand, was much more spread out. This could be due to another variable such as time after transplantation. Indeed, treatment time did appear to have some effect on the PCA since the ovoids representing 7 day-treatment samples were always to the left of the 1 or 3 days treatments.
    Figure Legend Snippet: The allograft T Cell microarray data were analyzed by PCA using Partek Genomics Suite. 50% of the variation in samples was revealed in the first two principal components. The first principal component is represented by the X axis and the second principal component by the Y axis. Treatment type appeared to be major source of variation and is indicated by different colored ellipsoids. Green, untreated; Red, CsA only; and Blue, CsA plus peptide. The numbers next to each ellipsoid indicate the number of days post transplantation regardless of treatment type. The type of treatment appeared to be the most significant effect and the treatment time was another significant variable. Each treatment could be seen as distinct groups. Both the untreated (Green) and the cyclosporine treated (Red) samples seemed to form similar cluster shapes. Cyclosporine plus peptide (Blue) sample, on the other hand, was much more spread out. This could be due to another variable such as time after transplantation. Indeed, treatment time did appear to have some effect on the PCA since the ovoids representing 7 day-treatment samples were always to the left of the 1 or 3 days treatments.

    Techniques Used: Microarray, Transplantation Assay

    Related Articles

    other:

    Article Title: Disruption of Trim9 function abrogates macrophage motility in vivo
    Article Snippet: RNA was labeled (Cy3) and hybridized to arrays using standard methods (Cogenics, Research Triangle Park, NC, USA).

    Microarray:

    Article Title: Classifiers of NF-κB pathway activation, devices, and methods of use thereof
    Article Snippet: The nCounterTM System Assay nCounter Analysis System technology was developed by NanoString Technologies. .. In brief, microarray hybridization data was prepared by Cogenics, Inc, using an Agilent 4X44 platform. .. Scanning and image analysis were performed using an Agilent 2100 Bioanalyzer and Agilent MR-2 DNA Microarray Scanner (Agilent Technologies, Inc).

    Article Title: Intragraft gene expression profile associated with the induction of tolerance by allochimeric MHC I in the rat heart transplantation model.
    Article Snippet: The MHC class I allochimeric protein containing donor-type epitopes on recipient-type heavy chains induces indefinite survival of heterotopic cardiac allografts in rats.. We analyzed gene expression profile of heart allograft tissue.. Mutated peptide [a1h1/u]-RT1.Aa that contains donor-type (Wistar Furth, WF; RT1u) immunogenic epitopes displayed on recipient-type (ACI, RT1a) was delivered into ACI recipients of WF hearts at the time of transplantation in addition to a 3 days course of oral cyclosporine.

    Article Title: Novel Regulators of the Systemic Response to Lipopolysaccharide
    Article Snippet: .. The authors thank Jessica Ramsberger, Tatiana Vinogradova, and Sara Weinke for help with animal studies and RNA extractions and Ed Lobenhofer (Cogenics) for assistance with microarray hybridization assays. ..

    Article Title: Transcriptional Profiling of Non-Human Primate Lymphoid Organ Responses to Total-Body Irradiation
    Article Snippet: .. Microarray procedures were performed by Cogenics (Morrisville, NC). .. Briefly, 1 lg of total RNA was assayed using GeneChipe Rhesus Macaque Genome Arrays (Affymetrixt Inc., Waltham, MA) and the GeneChip One-Cycle Target Labeling Kit (Affymetrix), according to the manufacturer’s recommended protocol.

    Article Title: The LO-BaFL method and ALS microarray expression analysis
    Article Snippet: The microarray experiment used Agilent 4x44K human genome microarrays [ ] in a pooled healthy control reference design [ ]. .. Sample and microarray processing were performed at Cogenics TM [ ], producing arrays contrasting each sample (healthy and diseased) to the reference. .. The raw data sent back by Cogenics includes extracted spot intensities and the background-subtracted intensity ratios for each contrast.

    Article Title: Arsenic- and Cadmium-Induced Toxicogenomic Response in Mouse Embryos Undergoing Neurulation
    Article Snippet: .. The authors wish to thank Dr Edward K. Lobenhofer and Cogenics for conducting microarray hybridization for all Affymetrix arrays used in this study and Alison Laing, Alison Scherer, and Erica Finsness for editorial support. ..

    Hybridization:

    Article Title: Classifiers of NF-κB pathway activation, devices, and methods of use thereof
    Article Snippet: The nCounterTM System Assay nCounter Analysis System technology was developed by NanoString Technologies. .. In brief, microarray hybridization data was prepared by Cogenics, Inc, using an Agilent 4X44 platform. .. Scanning and image analysis were performed using an Agilent 2100 Bioanalyzer and Agilent MR-2 DNA Microarray Scanner (Agilent Technologies, Inc).

    Article Title: Intragraft gene expression profile associated with the induction of tolerance by allochimeric MHC I in the rat heart transplantation model.
    Article Snippet: The MHC class I allochimeric protein containing donor-type epitopes on recipient-type heavy chains induces indefinite survival of heterotopic cardiac allografts in rats.. We analyzed gene expression profile of heart allograft tissue.. Mutated peptide [a1h1/u]-RT1.Aa that contains donor-type (Wistar Furth, WF; RT1u) immunogenic epitopes displayed on recipient-type (ACI, RT1a) was delivered into ACI recipients of WF hearts at the time of transplantation in addition to a 3 days course of oral cyclosporine.

    Article Title: Novel Regulators of the Systemic Response to Lipopolysaccharide
    Article Snippet: .. The authors thank Jessica Ramsberger, Tatiana Vinogradova, and Sara Weinke for help with animal studies and RNA extractions and Ed Lobenhofer (Cogenics) for assistance with microarray hybridization assays. ..

    Article Title: Reprogramming of human somatic cells using human and animal oocytes.
    Article Snippet: There is renewed interest in using animal oocytes to reprogram human somatic cells.. Here we compare the reprogramming of human somatic nuclei using oocytes obtained from animal and human sources.. Comparative analysis of gene expression in morula-stage embryos was carried out using single-embryo transcriptome amplification and global gene expression analyses.

    Article Title: Arsenic- and Cadmium-Induced Toxicogenomic Response in Mouse Embryos Undergoing Neurulation
    Article Snippet: .. The authors wish to thank Dr Edward K. Lobenhofer and Cogenics for conducting microarray hybridization for all Affymetrix arrays used in this study and Alison Laing, Alison Scherer, and Erica Finsness for editorial support. ..

    RNA Amplification:

    Article Title: Reprogramming of human somatic cells using human and animal oocytes.
    Article Snippet: There is renewed interest in using animal oocytes to reprogram human somatic cells.. Here we compare the reprogramming of human somatic nuclei using oocytes obtained from animal and human sources.. Comparative analysis of gene expression in morula-stage embryos was carried out using single-embryo transcriptome amplification and global gene expression analyses.



    Similar Products

    microarray hybridization and statistical analysis  (Genminix Informatics Co Ltd)
    90
    Genminix Informatics Co Ltd microarray hybridization and statistical analysis
    Microarray Hybridization And Statistical Analysis, supplied by Genminix Informatics Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microarray+hybridization+and+statistical+analysis/microarray+hybridization+and+statistical+analysis/pmc06422445-92-10-5
    Average 90 stars, based on 1 article reviews
    microarray hybridization and statistical analysis - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results