Review



mrna processing, microarray hybridisation and probe expression normalisation  (Arraystar inc)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90

    Structured Review

    Arraystar inc mrna processing, microarray hybridisation and probe expression normalisation
    Mrna Processing, Microarray Hybridisation And Probe Expression Normalisation, supplied by Arraystar inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microarray+hybridisation+and+data+normalisation+analyses/mrna+processing++microarray+hybridisation+and+probe+expression+normalisation/pmc04599578-153-27-17
    Average 90 stars, based on 1 article reviews
    mrna processing, microarray hybridisation and probe expression normalisation - by Bioz Stars, 2026-09
    90/100 stars

    Images

    Related Articles

    Microarray:

    Article Title: Neoplastic-like transformation effect of single-walled and multi-walled carbon nanotubes compared to asbestos on human lung small airway epithelial cells
    Article Snippet: All exposed and passage control SAECs were seeded in triplicate at 5 × 10 5 cells to 60-mm plates in normal growth medium and incubated in normal growth conditions for 1–2 d. Then, sub-confluent plates were lysed with ice-cold TRIzol Reagent (Invitrogen) following manufacturer's instructions to collect mRNA. .. Lysates were then immediately frozen at −80° C for 24 h and shipped on dry ice to ArrayStar (Rockville, MD, USA) for mRNA processing, microarray hybridisation and probe expression normalisation. .. On arrival, mRNA was extracted following manufacturer's protocol and evaluated for RNA quality and quantity using a Bioanalyzer 2100 (Agilent) and Nanodrop ND-1000. mRNA was then reverse-transcribed with Superscript double-stranded-cDNA synthesis kit (Invitrogen). cDNA samples were then amplified, Cy3-labeled with NimbleGen one-colour DNA labelling kit and hybridised to NimbleGen whole human genome 12 × 135k microarrays in NimbleGen Hybridisation System.

    Hybridization:

    Article Title: Neoplastic-like transformation effect of single-walled and multi-walled carbon nanotubes compared to asbestos on human lung small airway epithelial cells
    Article Snippet: All exposed and passage control SAECs were seeded in triplicate at 5 × 10 5 cells to 60-mm plates in normal growth medium and incubated in normal growth conditions for 1–2 d. Then, sub-confluent plates were lysed with ice-cold TRIzol Reagent (Invitrogen) following manufacturer's instructions to collect mRNA. .. Lysates were then immediately frozen at −80° C for 24 h and shipped on dry ice to ArrayStar (Rockville, MD, USA) for mRNA processing, microarray hybridisation and probe expression normalisation. .. On arrival, mRNA was extracted following manufacturer's protocol and evaluated for RNA quality and quantity using a Bioanalyzer 2100 (Agilent) and Nanodrop ND-1000. mRNA was then reverse-transcribed with Superscript double-stranded-cDNA synthesis kit (Invitrogen). cDNA samples were then amplified, Cy3-labeled with NimbleGen one-colour DNA labelling kit and hybridised to NimbleGen whole human genome 12 × 135k microarrays in NimbleGen Hybridisation System.

    Expressing:

    Article Title: Neoplastic-like transformation effect of single-walled and multi-walled carbon nanotubes compared to asbestos on human lung small airway epithelial cells
    Article Snippet: All exposed and passage control SAECs were seeded in triplicate at 5 × 10 5 cells to 60-mm plates in normal growth medium and incubated in normal growth conditions for 1–2 d. Then, sub-confluent plates were lysed with ice-cold TRIzol Reagent (Invitrogen) following manufacturer's instructions to collect mRNA. .. Lysates were then immediately frozen at −80° C for 24 h and shipped on dry ice to ArrayStar (Rockville, MD, USA) for mRNA processing, microarray hybridisation and probe expression normalisation. .. On arrival, mRNA was extracted following manufacturer's protocol and evaluated for RNA quality and quantity using a Bioanalyzer 2100 (Agilent) and Nanodrop ND-1000. mRNA was then reverse-transcribed with Superscript double-stranded-cDNA synthesis kit (Invitrogen). cDNA samples were then amplified, Cy3-labeled with NimbleGen one-colour DNA labelling kit and hybridised to NimbleGen whole human genome 12 × 135k microarrays in NimbleGen Hybridisation System.



    Similar Products

    90
    CapitalBio Corporation microarray hybridisation and data normalisation analyses
    Microarray Hybridisation And Data Normalisation Analyses, supplied by CapitalBio Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microarray+hybridisation+and+data+normalisation+analyses/microarray+hybridization+and+data+normalization+analysis/pmc03471800-67-4-10
    Average 90 stars, based on 1 article reviews
    microarray hybridisation and data normalisation analyses - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    Image Search Results