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snpware uht reagent kit  (Beckman Coulter)


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    Beckman Coulter snpware uht reagent kit
    Snpware Uht Reagent Kit, supplied by Beckman Coulter, used in various techniques. Bioz Stars score: 96/100, based on 1333 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microarray+human+scleroderma+data+set/Reagent+Kit/pmc04659097-53-33-54
    Average 96 stars, based on 1333 article reviews
    snpware uht reagent kit - by Bioz Stars, 2026-11
    96/100 stars

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    other:

    Article Title: Single-cell chromatin accessibility landscape of cardiac non-myocytes identifies tissue repair program during heart regeneration.
    Article Snippet: For low volumes, a 40 μm Flowmi Cell Strainer (H13680-0040, Bel-Art) was used to minimize volume loss.

    Article Title: Sensing of metabolic signals via GPR183 promotes occupation of lung macrophage niches by monocytes
    Article Snippet: Cells were sorted from single-cell suspensions using a FACS Aria II/III (BD Biosciences).

    Amplification:

    Article Title: Endothelial stem cells of the retinal vasculature reside in the optic nerve
    Article Snippet: .. The cDNA was then amplified using a thermal cycler, and the amplified cDNA product was purified using the SPRIselect Reagent Kit (Beckman Coulter, Brea, CA, USA). .. Gene expression libraries were constructed using Chromium Single Cell 3′ GEM, Library & Gel Bead Kit v3 or Chromium Next GEM Single Cell 5’ Kit v2 (10x Genomics, Pleasanton, CA, USA) following the manufacturer’s instructions.

    Article Title: Iron deficiency induces maturation-dependent loss of pancreatic β-cells.
    Article Snippet: For scRNA-seq of islet cells of 3-week-old CTRL and β-Tfrc-KO mice, GEMs and scRNA-seq libraries were prepared with the Chromium Next GEM Single Cell 3’ Kit v3.1, Chromium Next GEM Chip G Single Cell Kit, and the Dual Index Kit TT Set A according to the manufacturer’s instructions using the Chromium Controller (10x Genomics). .. GEM reverse transcription (GEM-RT) was carried out in a C1000 Touch Thermal Cycler (Bio-Rad) at 53°C for 45 min., followed by inactivation at 85°C for 5 min. Cleanup of the GEM-RT product was performed using Dynabeads MyOne SILANE reagent and the SPRIselect Reagent Kit (Beckman Coulter). cDNA amplification involved an initial denaturation at 98°C for 3 min., followed by 11 to 12 cycles at 98°C for 15 sec., 63°C for 20 sec., and 72°C for 1 min., with a final extension at 72°C for 1 min. ..

    Article Title: Endothelial stem cells of the retinal vasculature reside in the optic nerve.
    Article Snippet: .. The cDNA was then amplified using a thermal cycler, and the amplified cDNA product was purified using the SPRIselect Reagent Kit (Beckman Coulter, Brea, CA, USA). .. Gene expression libraries were constructed using Chromium Single Cell 3′ GEM, Library & Gel Bead Kit v3 or Chromium Next GEM Single Cell 5’ Kit v2 (10x Genomics, Pleasanton, CA, USA) following the manufacturer’s instructions.

    Purification:

    Article Title: Endothelial stem cells of the retinal vasculature reside in the optic nerve
    Article Snippet: .. The cDNA was then amplified using a thermal cycler, and the amplified cDNA product was purified using the SPRIselect Reagent Kit (Beckman Coulter, Brea, CA, USA). .. Gene expression libraries were constructed using Chromium Single Cell 3′ GEM, Library & Gel Bead Kit v3 or Chromium Next GEM Single Cell 5’ Kit v2 (10x Genomics, Pleasanton, CA, USA) following the manufacturer’s instructions.

    Article Title: Endothelial stem cells of the retinal vasculature reside in the optic nerve.
    Article Snippet: .. The cDNA was then amplified using a thermal cycler, and the amplified cDNA product was purified using the SPRIselect Reagent Kit (Beckman Coulter, Brea, CA, USA). .. Gene expression libraries were constructed using Chromium Single Cell 3′ GEM, Library & Gel Bead Kit v3 or Chromium Next GEM Single Cell 5’ Kit v2 (10x Genomics, Pleasanton, CA, USA) following the manufacturer’s instructions.

    Reverse Transcription:

    Article Title: Iron deficiency induces maturation-dependent loss of pancreatic β-cells.
    Article Snippet: For scRNA-seq of islet cells of 3-week-old CTRL and β-Tfrc-KO mice, GEMs and scRNA-seq libraries were prepared with the Chromium Next GEM Single Cell 3’ Kit v3.1, Chromium Next GEM Chip G Single Cell Kit, and the Dual Index Kit TT Set A according to the manufacturer’s instructions using the Chromium Controller (10x Genomics). .. GEM reverse transcription (GEM-RT) was carried out in a C1000 Touch Thermal Cycler (Bio-Rad) at 53°C for 45 min., followed by inactivation at 85°C for 5 min. Cleanup of the GEM-RT product was performed using Dynabeads MyOne SILANE reagent and the SPRIselect Reagent Kit (Beckman Coulter). cDNA amplification involved an initial denaturation at 98°C for 3 min., followed by 11 to 12 cycles at 98°C for 15 sec., 63°C for 20 sec., and 72°C for 1 min., with a final extension at 72°C for 1 min. ..

    Modification:

    Article Title: Cohort Profile Update: Survey of Health, Ageing and Retirement in Europe – Biomarker data for age-related health conditions
    Article Snippet: To measure HbA1c immunoturbidimetrically, a single 3.2mm punch was eluted in 300μL Haemolyzing Reagent (Beckman Coulter, Inc.) by shaking at 1000rpm for 1 hr. at RT. .. An aliquot of 200μL was transferred to a 0.3mL sample cup and loaded onto the DxC 600 (Beckman Coulter) and assayed using the HbA1c-reagent kit (Beckman Coulter A87905) without modification beyond the DBS sample type. ..



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    microarray human scleroderma data set  (Biotechnology Information)
    90
    Biotechnology Information microarray human scleroderma data set
    The IL-13 pathway is activated in SSc and sclGVHD skin. A: Gene expression data for the 491 IL-13–responsive genes identified in human dermal fibroblasts were extracted from the SSc skin data of Milano et al.4 SSc skin biopsy samples are ordered by intrinsic subset and genes are organized by hierarchical clustering. The centroid average of the IL-13–responsive gene signature at maximal induction (12 and 24 hours) is shown to the left of the heat map. Pearson's correlation coefficients between the centroid and individual patient sample are plotted below each array. B: Expression data for the 734 genes reported by Fulkerson et al26 as IL-13–inducible genes in mouse lung were extracted from the sclGVHD <t>microarray</t> data set obtained 2 weeks and 5 weeks after splenocyte transfer (n = 4 per group). Genes and arrays were organized by hierarchical clustering. The relative expression of IL-13–inducible genes (centroid) in lungs is shown to the left of the heat map. C: Representative IL-13 IHC on skin biopsies obtained from an SSc patient and a normal control subject. Arrowheads indicate IL-13+ cells. Original magnification, ×400. D: Blinded quantification of the number of IHC IL-13+ cells per high-power field (×600) in skin biopsies from SSc patients and normal control subjects. SSc, n = 18; control, n = 6; P = 0.0037. E: IL-13 ELISA on the tissue culture supernatants of skin explants from BALB/c Rag2−/− mice that received either syngeneic BALB/c (n = 3) or allogeneic B10.D2 (n = 3) splenocytes 2 weeks earlier (P = 0.002).
    Microarray Human Scleroderma Data Set, supplied by Biotechnology Information, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microarray+human+scleroderma+data+set/microarray+human+scleroderma+data+set/pmc03349888-203-2-18
    Average 90 stars, based on 1 article reviews
    microarray human scleroderma data set - by Bioz Stars, 2026-11
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    The IL-13 pathway is activated in SSc and sclGVHD skin. A: Gene expression data for the 491 IL-13–responsive genes identified in human dermal fibroblasts were extracted from the SSc skin data of Milano et al.4 SSc skin biopsy samples are ordered by intrinsic subset and genes are organized by hierarchical clustering. The centroid average of the IL-13–responsive gene signature at maximal induction (12 and 24 hours) is shown to the left of the heat map. Pearson's correlation coefficients between the centroid and individual patient sample are plotted below each array. B: Expression data for the 734 genes reported by Fulkerson et al26 as IL-13–inducible genes in mouse lung were extracted from the sclGVHD microarray data set obtained 2 weeks and 5 weeks after splenocyte transfer (n = 4 per group). Genes and arrays were organized by hierarchical clustering. The relative expression of IL-13–inducible genes (centroid) in lungs is shown to the left of the heat map. C: Representative IL-13 IHC on skin biopsies obtained from an SSc patient and a normal control subject. Arrowheads indicate IL-13+ cells. Original magnification, ×400. D: Blinded quantification of the number of IHC IL-13+ cells per high-power field (×600) in skin biopsies from SSc patients and normal control subjects. SSc, n = 18; control, n = 6; P = 0.0037. E: IL-13 ELISA on the tissue culture supernatants of skin explants from BALB/c Rag2−/− mice that received either syngeneic BALB/c (n = 3) or allogeneic B10.D2 (n = 3) splenocytes 2 weeks earlier (P = 0.002).

    Journal: The American Journal of Pathology

    Article Title: Interspecies Comparison of Human and Murine Scleroderma Reveals IL-13 and CCL2 as Disease Subset-Specific Targets

    doi: 10.1016/j.ajpath.2011.11.024

    Figure Lengend Snippet: The IL-13 pathway is activated in SSc and sclGVHD skin. A: Gene expression data for the 491 IL-13–responsive genes identified in human dermal fibroblasts were extracted from the SSc skin data of Milano et al.4 SSc skin biopsy samples are ordered by intrinsic subset and genes are organized by hierarchical clustering. The centroid average of the IL-13–responsive gene signature at maximal induction (12 and 24 hours) is shown to the left of the heat map. Pearson's correlation coefficients between the centroid and individual patient sample are plotted below each array. B: Expression data for the 734 genes reported by Fulkerson et al26 as IL-13–inducible genes in mouse lung were extracted from the sclGVHD microarray data set obtained 2 weeks and 5 weeks after splenocyte transfer (n = 4 per group). Genes and arrays were organized by hierarchical clustering. The relative expression of IL-13–inducible genes (centroid) in lungs is shown to the left of the heat map. C: Representative IL-13 IHC on skin biopsies obtained from an SSc patient and a normal control subject. Arrowheads indicate IL-13+ cells. Original magnification, ×400. D: Blinded quantification of the number of IHC IL-13+ cells per high-power field (×600) in skin biopsies from SSc patients and normal control subjects. SSc, n = 18; control, n = 6; P = 0.0037. E: IL-13 ELISA on the tissue culture supernatants of skin explants from BALB/c Rag2−/− mice that received either syngeneic BALB/c (n = 3) or allogeneic B10.D2 (n = 3) splenocytes 2 weeks earlier (P = 0.002).

    Article Snippet: The 75 microarray human scleroderma data set, previously described, 4 is publicly available at the National Center for Biotechnology Information GEO site ( http://www.ncbi.nlm.nih.gov/geo ; accession {"type":"entrez-geo","attrs":{"text":"GSE9285","term_id":"9285"}} GSE9285 ).

    Techniques: Gene Expression, Expressing, Microarray, Control, Enzyme-linked Immunosorbent Assay