Review



pbs thermo fisher scientific  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier
Bioz Manufacturer Symbol Thermo Fisher manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 99
      Buy from Supplier

    Structured Review

    Thermo Fisher pbs thermo fisher scientific
    Pbs Thermo Fisher Scientific, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microarray+human+scleroderma+data+set/PBS/pm30096312-291-71-72
    Average 99 stars, based on 1 article reviews
    pbs thermo fisher scientific - by Bioz Stars, 2026-09
    99/100 stars

    Images

    Related Articles

    other:

    Article Title: Derivatives of dolaproine-dolaisoleuine peptides
    Article Snippet: After 72 h of acclimatization, the mice are implanted with SW780 human bladder cancer cells (2×106 cells/mouse), suspended in 50% complete cultrex (Trevigen, Inc.) mixed with PBS (Gibco), and the tumor growth rate is monitored.

    Incubation:

    Article Title: Biomineralized outer membrane vesicles for synergistic immuno-photodynamic therapy of oral squamous cell carcinoma
    Article Snippet: .. First, OMVs containing 1 mg of total protein were dispersed in 1 mL of PBS (Gibco) and incubated overnight in a constant-temperature shaking incubator (200 rpm) at 37 °C to reach equilibrium. ..

    Article Title: Compound design of a patient-derived 3D cell culture system modelling early peritoneal endometriosis
    Article Snippet: Cells were permeabilised with 0.25% (v/v) Triton X-100 (Thermo Fisher Scientific, 85111) in PBS for 10 min at RT and blocked with 2% (w/v) bovine serum albumin (BSA; Sigma-Aldrich, A3803) for 1 h at RT. .. Cells were incubated with primary antibodies overnight at 4°C , followed by three 15 min PBS washes and incubation with secondary antibodies AlexaFluorTM 488 goat-anti-mouse IgG1 (Invitrogen, A21121) and AlexaFluorTM 568 goat-anti-rabbit (Invitrogen, A11011) at 1:1000 dilution, and DAPI (Sigma-Aldrich, D9542) in a 1:500 dilution, for 1 h at RT. .. The slides were mounted using FluoromountTM (Invitrogen, 00-4958-02).

    Article Title: Immunomodulatory topographies regulate myofibroblast differentiation and influence fibrous encapsulation of glaucoma drainage devices
    Article Snippet: Next, the cells were permeabilized with 0.5 % Triton X-100 (Merck) dissolved in PBS for 10 min and blocked with 3 % (w/v) bovine serum albumin (Merck) in PBS for 30 min and then with 5 % (v/v) goat serum (Fisher Scientific) dissolved in PBS for 30 min. Next, cells were incubated with a primary antibody using mouse monoclonal anti α-smooth muscle actin diluted in PBS (1:600; Sigma: A5228) overnight at 4 °C. .. The next day, the cells were washed 3 times with PBS and then incubated with a secondary antibody goat anti-mouse Alexa Flour 488 diluted in PBS (1:500; Molecular probes: A21121) and incubated in the dark for 1 h at room temperature. .. For the α-SMA screen, cells were washed three times with PBS and stained with phalloidin-TRITC diluted in PBS (1:200, ThermoFisher) for 45 min and washed 3 times again with PBS and stained with 4′,6-Diamidino-2-Phenylindole (DAPI) (Sigma Aldrich; 1:500) for 15 min. For the macrophage screen, the cell membrane was stained using CellMaskTM orange (1:1000; ThermoFisher) for 10 min at room temperature.

    Staining:

    Article Title: Immunomodulatory topographies regulate myofibroblast differentiation and influence fibrous encapsulation of glaucoma drainage devices
    Article Snippet: The next day, the cells were washed 3 times with PBS and then incubated with a secondary antibody goat anti-mouse Alexa Flour 488 diluted in PBS (1:500; Molecular probes: A21121) and incubated in the dark for 1 h at room temperature. .. For the α-SMA screen, cells were washed three times with PBS and stained with phalloidin-TRITC diluted in PBS (1:200, ThermoFisher) for 45 min and washed 3 times again with PBS and stained with 4′,6-Diamidino-2-Phenylindole (DAPI) (Sigma Aldrich; 1:500) for 15 min. For the macrophage screen, the cell membrane was stained using CellMaskTM orange (1:1000; ThermoFisher) for 10 min at room temperature. .. Afterward, cells were washed with PBS 3 times and stained with DAPI (Sigma Aldrich; 1:500) for 15 min. After staining, all the surfaces were washed three times with PBS after which they were mounted onto a glass slide using Mowiol (Sigma).

    Membrane:

    Article Title: Immunomodulatory topographies regulate myofibroblast differentiation and influence fibrous encapsulation of glaucoma drainage devices
    Article Snippet: The next day, the cells were washed 3 times with PBS and then incubated with a secondary antibody goat anti-mouse Alexa Flour 488 diluted in PBS (1:500; Molecular probes: A21121) and incubated in the dark for 1 h at room temperature. .. For the α-SMA screen, cells were washed three times with PBS and stained with phalloidin-TRITC diluted in PBS (1:200, ThermoFisher) for 45 min and washed 3 times again with PBS and stained with 4′,6-Diamidino-2-Phenylindole (DAPI) (Sigma Aldrich; 1:500) for 15 min. For the macrophage screen, the cell membrane was stained using CellMaskTM orange (1:1000; ThermoFisher) for 10 min at room temperature. .. Afterward, cells were washed with PBS 3 times and stained with DAPI (Sigma Aldrich; 1:500) for 15 min. After staining, all the surfaces were washed three times with PBS after which they were mounted onto a glass slide using Mowiol (Sigma).

    Sequencing:

    Article Title: Haplotype-resolved genome assemblies of BJ and IMR-90 human fibroblast cell lines reveal extensive structural variation and enable reanalysis of historical sequencing data
    Article Snippet: Both cell lines were authenticated by STR profiling at ATCC ( https://www.atcc.org/ , last accessed 27 February 2026). .. Cells for sequencing were harvested with 0.25% Trypsin–ethylenediaminetetraacetic acid (EDTA) (Thermo Fisher, Cat. No. 25200072), washed with 1× PBS (pH 7.4; Thermo Fisher, Cat. No. 10010023), counted on a CountessTM II FL Automated Cell Counter (Thermo Fisher, Cat. No. AMQAF1000), and used as input for NEB Monarch HMW genomic DNA (gDNA) isolation, Hi-C crosslinking, RNeasy Plus Mini isolation, or frozen as dry pellets and stored at −80°C for use in Oxford Nanopore Technologies (ONT) Ultra-Long (UL) library preparations. .. Estimated cumulative population doubling level (PDL) calculations were performed using PDL = 3.32 (logXe-logXb) + S where log is the base 10 logarithm, Xb is the cell number at the beginning of the incubation period, Xe is the cell number at the end of the incubation period, and S is the starting PDL (ref ATCC Animal Cell Culture Guide ACCG-042024-v08, p6).

    Isolation:

    Article Title: Haplotype-resolved genome assemblies of BJ and IMR-90 human fibroblast cell lines reveal extensive structural variation and enable reanalysis of historical sequencing data
    Article Snippet: Both cell lines were authenticated by STR profiling at ATCC ( https://www.atcc.org/ , last accessed 27 February 2026). .. Cells for sequencing were harvested with 0.25% Trypsin–ethylenediaminetetraacetic acid (EDTA) (Thermo Fisher, Cat. No. 25200072), washed with 1× PBS (pH 7.4; Thermo Fisher, Cat. No. 10010023), counted on a CountessTM II FL Automated Cell Counter (Thermo Fisher, Cat. No. AMQAF1000), and used as input for NEB Monarch HMW genomic DNA (gDNA) isolation, Hi-C crosslinking, RNeasy Plus Mini isolation, or frozen as dry pellets and stored at −80°C for use in Oxford Nanopore Technologies (ONT) Ultra-Long (UL) library preparations. .. Estimated cumulative population doubling level (PDL) calculations were performed using PDL = 3.32 (logXe-logXb) + S where log is the base 10 logarithm, Xb is the cell number at the beginning of the incubation period, Xe is the cell number at the end of the incubation period, and S is the starting PDL (ref ATCC Animal Cell Culture Guide ACCG-042024-v08, p6).

    Hi-C:

    Article Title: Haplotype-resolved genome assemblies of BJ and IMR-90 human fibroblast cell lines reveal extensive structural variation and enable reanalysis of historical sequencing data
    Article Snippet: Both cell lines were authenticated by STR profiling at ATCC ( https://www.atcc.org/ , last accessed 27 February 2026). .. Cells for sequencing were harvested with 0.25% Trypsin–ethylenediaminetetraacetic acid (EDTA) (Thermo Fisher, Cat. No. 25200072), washed with 1× PBS (pH 7.4; Thermo Fisher, Cat. No. 10010023), counted on a CountessTM II FL Automated Cell Counter (Thermo Fisher, Cat. No. AMQAF1000), and used as input for NEB Monarch HMW genomic DNA (gDNA) isolation, Hi-C crosslinking, RNeasy Plus Mini isolation, or frozen as dry pellets and stored at −80°C for use in Oxford Nanopore Technologies (ONT) Ultra-Long (UL) library preparations. .. Estimated cumulative population doubling level (PDL) calculations were performed using PDL = 3.32 (logXe-logXb) + S where log is the base 10 logarithm, Xb is the cell number at the beginning of the incubation period, Xe is the cell number at the end of the incubation period, and S is the starting PDL (ref ATCC Animal Cell Culture Guide ACCG-042024-v08, p6).



    Similar Products

    microarray human scleroderma data set  (Biotechnology Information)
    90
    Biotechnology Information microarray human scleroderma data set
    The IL-13 pathway is activated in SSc and sclGVHD skin. A: Gene expression data for the 491 IL-13–responsive genes identified in human dermal fibroblasts were extracted from the SSc skin data of Milano et al.4 SSc skin biopsy samples are ordered by intrinsic subset and genes are organized by hierarchical clustering. The centroid average of the IL-13–responsive gene signature at maximal induction (12 and 24 hours) is shown to the left of the heat map. Pearson's correlation coefficients between the centroid and individual patient sample are plotted below each array. B: Expression data for the 734 genes reported by Fulkerson et al26 as IL-13–inducible genes in mouse lung were extracted from the sclGVHD <t>microarray</t> data set obtained 2 weeks and 5 weeks after splenocyte transfer (n = 4 per group). Genes and arrays were organized by hierarchical clustering. The relative expression of IL-13–inducible genes (centroid) in lungs is shown to the left of the heat map. C: Representative IL-13 IHC on skin biopsies obtained from an SSc patient and a normal control subject. Arrowheads indicate IL-13+ cells. Original magnification, ×400. D: Blinded quantification of the number of IHC IL-13+ cells per high-power field (×600) in skin biopsies from SSc patients and normal control subjects. SSc, n = 18; control, n = 6; P = 0.0037. E: IL-13 ELISA on the tissue culture supernatants of skin explants from BALB/c Rag2−/− mice that received either syngeneic BALB/c (n = 3) or allogeneic B10.D2 (n = 3) splenocytes 2 weeks earlier (P = 0.002).
    Microarray Human Scleroderma Data Set, supplied by Biotechnology Information, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microarray+human+scleroderma+data+set/microarray+human+scleroderma+data+set/pmc03349888-203-2-18
    Average 90 stars, based on 1 article reviews
    microarray human scleroderma data set - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    The IL-13 pathway is activated in SSc and sclGVHD skin. A: Gene expression data for the 491 IL-13–responsive genes identified in human dermal fibroblasts were extracted from the SSc skin data of Milano et al.4 SSc skin biopsy samples are ordered by intrinsic subset and genes are organized by hierarchical clustering. The centroid average of the IL-13–responsive gene signature at maximal induction (12 and 24 hours) is shown to the left of the heat map. Pearson's correlation coefficients between the centroid and individual patient sample are plotted below each array. B: Expression data for the 734 genes reported by Fulkerson et al26 as IL-13–inducible genes in mouse lung were extracted from the sclGVHD microarray data set obtained 2 weeks and 5 weeks after splenocyte transfer (n = 4 per group). Genes and arrays were organized by hierarchical clustering. The relative expression of IL-13–inducible genes (centroid) in lungs is shown to the left of the heat map. C: Representative IL-13 IHC on skin biopsies obtained from an SSc patient and a normal control subject. Arrowheads indicate IL-13+ cells. Original magnification, ×400. D: Blinded quantification of the number of IHC IL-13+ cells per high-power field (×600) in skin biopsies from SSc patients and normal control subjects. SSc, n = 18; control, n = 6; P = 0.0037. E: IL-13 ELISA on the tissue culture supernatants of skin explants from BALB/c Rag2−/− mice that received either syngeneic BALB/c (n = 3) or allogeneic B10.D2 (n = 3) splenocytes 2 weeks earlier (P = 0.002).

    Journal: The American Journal of Pathology

    Article Title: Interspecies Comparison of Human and Murine Scleroderma Reveals IL-13 and CCL2 as Disease Subset-Specific Targets

    doi: 10.1016/j.ajpath.2011.11.024

    Figure Lengend Snippet: The IL-13 pathway is activated in SSc and sclGVHD skin. A: Gene expression data for the 491 IL-13–responsive genes identified in human dermal fibroblasts were extracted from the SSc skin data of Milano et al.4 SSc skin biopsy samples are ordered by intrinsic subset and genes are organized by hierarchical clustering. The centroid average of the IL-13–responsive gene signature at maximal induction (12 and 24 hours) is shown to the left of the heat map. Pearson's correlation coefficients between the centroid and individual patient sample are plotted below each array. B: Expression data for the 734 genes reported by Fulkerson et al26 as IL-13–inducible genes in mouse lung were extracted from the sclGVHD microarray data set obtained 2 weeks and 5 weeks after splenocyte transfer (n = 4 per group). Genes and arrays were organized by hierarchical clustering. The relative expression of IL-13–inducible genes (centroid) in lungs is shown to the left of the heat map. C: Representative IL-13 IHC on skin biopsies obtained from an SSc patient and a normal control subject. Arrowheads indicate IL-13+ cells. Original magnification, ×400. D: Blinded quantification of the number of IHC IL-13+ cells per high-power field (×600) in skin biopsies from SSc patients and normal control subjects. SSc, n = 18; control, n = 6; P = 0.0037. E: IL-13 ELISA on the tissue culture supernatants of skin explants from BALB/c Rag2−/− mice that received either syngeneic BALB/c (n = 3) or allogeneic B10.D2 (n = 3) splenocytes 2 weeks earlier (P = 0.002).

    Article Snippet: The 75 microarray human scleroderma data set, previously described, 4 is publicly available at the National Center for Biotechnology Information GEO site ( http://www.ncbi.nlm.nih.gov/geo ; accession {"type":"entrez-geo","attrs":{"text":"GSE9285","term_id":"9285"}} GSE9285 ).

    Techniques: Gene Expression, Expressing, Microarray, Control, Enzyme-linked Immunosorbent Assay