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magnetic mrna isolation kit  (New England Biolabs)


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    New England Biolabs magnetic mrna isolation kit
    ( A ) The concentration of NF-L (pg/mL) in the supernatant when NB-1 cells were cocultured with HAM-PBMCs ( n = 7) or HD-PBMCs ( n = 7). ( B ) The concentration of NF-L (pg/mL) in the supernatant when NB-1 cells were cocultured with HAM-PBMCs ( n = 9) and with mogamulizumab (antiCCR4) in a dose-dependent manner for 72 hours. ( C ) The comparison <t>of</t> <t>RGMA</t> <t>mRNA</t> gene expression levels using DNA microarray among normal CD4 + T cells (HD CD4 + : n = 4), HAM patient–derived CD4 + T cells (HAM CD4 + : n = 4), ACs ( n = 2), and smoldering/chronic-type-ATL patient–derived ( n = 3) HTLV-1–infected CD4 + T cells (Non-HAM infected CD4 + T cells: n = 5), and acute-type-ATL patient–derived HTLV-1–infected CD4 + T cells (Acute ATL infected cells: n = 3). ( D ) The comparison of the expression levels of the genes associated with the inhibition of neuroregeneration ( OMG , MAG , RTN4 , and WNT5A ) between HD CD4 + ( n = 4) and HAM CD4 + T cells ( n = 4). ( E ) The enrichment levels of H3K27me3 –2916 bp upstream from the TSS of the RGMA gene locus in HD CD4 + ( n = 3), HAM CD4 + ( n = 4), and acute-ATL infected cells ( n = 4). Data are shown as mean ± SD. ** P < 0.01; *** P < 0.001 by unpaired t test ( A and D ) or 1-way ANOVA with Dunnett’s multiple-comparison test ( B , C , and E ). NF-L, neurofilament light chain.
    Magnetic Mrna Isolation Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 617 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microarray+human+scleroderma+data+set/Magnetic+mRNA+Isolation+Kit/pmc12220939-154-21-25
    Average 97 stars, based on 617 article reviews
    magnetic mrna isolation kit - by Bioz Stars, 2026-10
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    Images

    1) Product Images from "Virus-induced RGMa expression drives neurodegeneration in HTLV-1–associated myelopathy"

    Article Title: Virus-induced RGMa expression drives neurodegeneration in HTLV-1–associated myelopathy

    Journal: JCI Insight

    doi: 10.1172/jci.insight.184530

    ( A ) The concentration of NF-L (pg/mL) in the supernatant when NB-1 cells were cocultured with HAM-PBMCs ( n = 7) or HD-PBMCs ( n = 7). ( B ) The concentration of NF-L (pg/mL) in the supernatant when NB-1 cells were cocultured with HAM-PBMCs ( n = 9) and with mogamulizumab (antiCCR4) in a dose-dependent manner for 72 hours. ( C ) The comparison of RGMA mRNA gene expression levels using DNA microarray among normal CD4 + T cells (HD CD4 + : n = 4), HAM patient–derived CD4 + T cells (HAM CD4 + : n = 4), ACs ( n = 2), and smoldering/chronic-type-ATL patient–derived ( n = 3) HTLV-1–infected CD4 + T cells (Non-HAM infected CD4 + T cells: n = 5), and acute-type-ATL patient–derived HTLV-1–infected CD4 + T cells (Acute ATL infected cells: n = 3). ( D ) The comparison of the expression levels of the genes associated with the inhibition of neuroregeneration ( OMG , MAG , RTN4 , and WNT5A ) between HD CD4 + ( n = 4) and HAM CD4 + T cells ( n = 4). ( E ) The enrichment levels of H3K27me3 –2916 bp upstream from the TSS of the RGMA gene locus in HD CD4 + ( n = 3), HAM CD4 + ( n = 4), and acute-ATL infected cells ( n = 4). Data are shown as mean ± SD. ** P < 0.01; *** P < 0.001 by unpaired t test ( A and D ) or 1-way ANOVA with Dunnett’s multiple-comparison test ( B , C , and E ). NF-L, neurofilament light chain.
    Figure Legend Snippet: ( A ) The concentration of NF-L (pg/mL) in the supernatant when NB-1 cells were cocultured with HAM-PBMCs ( n = 7) or HD-PBMCs ( n = 7). ( B ) The concentration of NF-L (pg/mL) in the supernatant when NB-1 cells were cocultured with HAM-PBMCs ( n = 9) and with mogamulizumab (antiCCR4) in a dose-dependent manner for 72 hours. ( C ) The comparison of RGMA mRNA gene expression levels using DNA microarray among normal CD4 + T cells (HD CD4 + : n = 4), HAM patient–derived CD4 + T cells (HAM CD4 + : n = 4), ACs ( n = 2), and smoldering/chronic-type-ATL patient–derived ( n = 3) HTLV-1–infected CD4 + T cells (Non-HAM infected CD4 + T cells: n = 5), and acute-type-ATL patient–derived HTLV-1–infected CD4 + T cells (Acute ATL infected cells: n = 3). ( D ) The comparison of the expression levels of the genes associated with the inhibition of neuroregeneration ( OMG , MAG , RTN4 , and WNT5A ) between HD CD4 + ( n = 4) and HAM CD4 + T cells ( n = 4). ( E ) The enrichment levels of H3K27me3 –2916 bp upstream from the TSS of the RGMA gene locus in HD CD4 + ( n = 3), HAM CD4 + ( n = 4), and acute-ATL infected cells ( n = 4). Data are shown as mean ± SD. ** P < 0.01; *** P < 0.001 by unpaired t test ( A and D ) or 1-way ANOVA with Dunnett’s multiple-comparison test ( B , C , and E ). NF-L, neurofilament light chain.

    Techniques Used: Concentration Assay, Comparison, Gene Expression, Microarray, Derivative Assay, Infection, Expressing, Inhibition

    ( A ) The validation of RGMA mRNA gene expression levels using qRT-PCR in HD CD4 + ( n = 6) and HAM CD4 + T cells ( n = 6). ( B ) Expression of RGMa protein in CD3 + CD4 + CCR4 + T cells from HAM-PBMCs. Representative dot plots of CCR4 and normal goat IgG (upper) or RGMa expression (bottom) in CD3 + CD4 + gated cells from HD-PBMCs (left) or HAM-PBMCs (right) cultured for 2 days. ( C ) Graph shows the percentage of RGMa protein–expressing cells in CCR4 – cells or CCR4 + cells in CD3 + CD4 + gated cells from HAM-PBMCs ( n = 8) cultured for 2 days, compared with the isotype control, normal goat IgG. ( D ) Graph shows the percentage of RGMa protein–expressing cells among CD3 + CD4 + CCR4 + gated cells from HD-PBMCs ( n = 5) or HAM-PBMCs ( n = 8) cultured for 2 days. Data are shown as mean ± SD. * P < 0.05; ** P < 0.01 by unpaired t test ( A and D ) or 1-way ANOVA with Dunnett’s multiple-comparison test.
    Figure Legend Snippet: ( A ) The validation of RGMA mRNA gene expression levels using qRT-PCR in HD CD4 + ( n = 6) and HAM CD4 + T cells ( n = 6). ( B ) Expression of RGMa protein in CD3 + CD4 + CCR4 + T cells from HAM-PBMCs. Representative dot plots of CCR4 and normal goat IgG (upper) or RGMa expression (bottom) in CD3 + CD4 + gated cells from HD-PBMCs (left) or HAM-PBMCs (right) cultured for 2 days. ( C ) Graph shows the percentage of RGMa protein–expressing cells in CCR4 – cells or CCR4 + cells in CD3 + CD4 + gated cells from HAM-PBMCs ( n = 8) cultured for 2 days, compared with the isotype control, normal goat IgG. ( D ) Graph shows the percentage of RGMa protein–expressing cells among CD3 + CD4 + CCR4 + gated cells from HD-PBMCs ( n = 5) or HAM-PBMCs ( n = 8) cultured for 2 days. Data are shown as mean ± SD. * P < 0.05; ** P < 0.01 by unpaired t test ( A and D ) or 1-way ANOVA with Dunnett’s multiple-comparison test.

    Techniques Used: Biomarker Discovery, Gene Expression, Quantitative RT-PCR, Expressing, Cell Culture, Control, Comparison

    ( A ) Tax (left), HBZ (middle), and RGMA (right) gene expression levels in cultured HAM-PBMCs ( n = 7) in a time-dependent manner. RPL19 was used as an internal control. ( B ) Tax-dependent RGMA mRNA gene induction in Jurkat cells, which were infected with lentivirus carrying the Tax gene. Top: Tax expression in the Jurkat cells was confirmed by Western blotting. β-Actin was measured as an internal control. Bottom: The induction levels of the RGMA gene were evaluated by qRT-PCR in a time-dependent manner ( n = 3). ( C ) Tax -dependent RGMA mRNA gene induction in JPX9 cells treated with 20 μM CdCl 2 in a time-dependent manner. Tax mRNA (upper) and RGMA mRNA (bottom) were measured by qRT-PCR ( n = 3). GAPDH was measured as an internal control. ( D ) Tax-dependent RGMa protein induction in JPX9 cells treated with 20 μM CdCl 2 for 3 days. Dot plots of Tax and normal goat IgG (upper) or RGMa expression (bottom) in JPX9 cells. JPX9(-), untreated JPX9 cells; 20 μM CdCl 2 JPX9, CdCl 2 -supplemented JPX9 cells. Data are shown as mean ± SD. * P < 0.05; ** P < 0.01; *** P < 0.001 by 1-way ANOVA with Dunnett’s multiple-comparison test ( A ), 2-sided Student’s t test ( B ), or an unpaired t test ( C ). Experiments were performed in triplicate ( B and C ).
    Figure Legend Snippet: ( A ) Tax (left), HBZ (middle), and RGMA (right) gene expression levels in cultured HAM-PBMCs ( n = 7) in a time-dependent manner. RPL19 was used as an internal control. ( B ) Tax-dependent RGMA mRNA gene induction in Jurkat cells, which were infected with lentivirus carrying the Tax gene. Top: Tax expression in the Jurkat cells was confirmed by Western blotting. β-Actin was measured as an internal control. Bottom: The induction levels of the RGMA gene were evaluated by qRT-PCR in a time-dependent manner ( n = 3). ( C ) Tax -dependent RGMA mRNA gene induction in JPX9 cells treated with 20 μM CdCl 2 in a time-dependent manner. Tax mRNA (upper) and RGMA mRNA (bottom) were measured by qRT-PCR ( n = 3). GAPDH was measured as an internal control. ( D ) Tax-dependent RGMa protein induction in JPX9 cells treated with 20 μM CdCl 2 for 3 days. Dot plots of Tax and normal goat IgG (upper) or RGMa expression (bottom) in JPX9 cells. JPX9(-), untreated JPX9 cells; 20 μM CdCl 2 JPX9, CdCl 2 -supplemented JPX9 cells. Data are shown as mean ± SD. * P < 0.05; ** P < 0.01; *** P < 0.001 by 1-way ANOVA with Dunnett’s multiple-comparison test ( A ), 2-sided Student’s t test ( B ), or an unpaired t test ( C ). Experiments were performed in triplicate ( B and C ).

    Techniques Used: Gene Expression, Cell Culture, Control, Infection, Expressing, Western Blot, Quantitative RT-PCR, Comparison

    Related Articles

    Purification:

    Article Title: m 6 A mRNA methylation regulates pancreatic α-cell plasticity.
    Article Snippet: .. mRNA was purified from total RNA using the Magnetic mRNA Isolation Kit (#S1550, NEB). .. Equal amounts of purified mRNA were spotted onto a nylon membrane (#77016, Thermo Fisher Scientific) and UV-crosslinked at 254 nm (0.12 J cm−2).

    Article Title: Programmable RNA N 6 ,2´-O-Dimethyladenosine Editing.
    Article Snippet: .. 200 μg of total RNA was subjected to two rounds of Poly(A) mRNA purification using the magnetic mRNA isolation kit from (NEB). ..

    Isolation:

    Article Title: m 6 A mRNA methylation regulates pancreatic α-cell plasticity.
    Article Snippet: .. mRNA was purified from total RNA using the Magnetic mRNA Isolation Kit (#S1550, NEB). .. Equal amounts of purified mRNA were spotted onto a nylon membrane (#77016, Thermo Fisher Scientific) and UV-crosslinked at 254 nm (0.12 J cm−2).

    Article Title: TOR-dependent regulation of the yeast homolog of the juvenile Batten Disease-associated gene CLN3
    Article Snippet: The RNA concentration was measured using a NanoDrop spectrophotometer (ThermoFisher Scientific) at 260 nm. .. Poly-A RNA was isolated from a total of 80 μ g of RNA using oligo d(T)25 magnetic beads (New England Biolabs, 61002), following the protocol and kit purchased from New England Biolabs (S1550). ..

    Article Title: Programmable RNA N 6 ,2´-O-Dimethyladenosine Editing.
    Article Snippet: .. 200 μg of total RNA was subjected to two rounds of Poly(A) mRNA purification using the magnetic mRNA isolation kit from (NEB). ..

    Article Title: EffectorGeneP: accurate gene annotation in pathogen genomes from infection transcriptomes
    Article Snippet: .. Messenger (m)RNA was extracted from transformed protoplasts after 24 hours using the NEB Magnetic mRNA Isolation Kit (#S1550S). .. Library-specific cDNA synthesis and PCR was carried out on 30 ng mRNA using the Invitrogen SuperScript IV One-Step RT-PCR System with ezDNase kit (Cat. # 12595100) with the forward and reverse primers ZmUbi1_5UTR_F3b (5’GCACACACACACAACCAG-3’) and FS_cDNA_R (5’- TGCTAGATCTCGACAGTACG-3’) as previously described .

    Article Title: A massively parallel reporter assay of MECP2 cis-regulatory elements reveals genetic candidates for male-biased autism
    Article Snippet: RNA and DNA were isolated from each replicate using the Qiagen AllPrep kit (Qiagen 80204). .. To prepare sequencing libraries, RNA was first treated with TURBO DNase (Thermo Fisher Scientific AM2239) to remove any remaining DNA, and then polyA-tailed mRNA was isolated with oligo d(T) beads (NEB S1550S). .. 0.7μg of RNA was then reverse-transcribed using SuperScript III RT (Thermo Fisher Scientific 18080093), using the P7-pLSmP-ass16UMI-gfp primer to add unique molecular identifiers to the cDNA.

    Incubation:

    Article Title: RNA methyltransferase NSUN2 enhances vasculogenic mimicry and malignant progression of cervical cancer through upregulation of MMP-9
    Article Snippet: .. Total RNA was incubated with oligo dT(25) beads (cat. no. S1550S; New England Biolabs, Inc.) for 10 min at RT and then washed with Lysis/Binding buffer (cat. no. B1556AVIAL; New England Biolabs, Inc.), Wash Buffer I (cat. no. B1558AVIAL; New England Biolabs, Inc.), Wash Buffer II (cat. no. B1559AVIAL; New England Biolabs, Inc.)and Low Salt Buffer (cat. no. B1557AVIAL; New England Biolabs, Inc.). .. Total RNA was incubated with oligo dT(25) beads (cat. no. S1550S; New England Biolabs, Inc.) for 10 min at RT and then washed with Lysis/Binding buffer (cat. no. B1556AVIAL; New England Biolabs, Inc.), Wash Buffer I (cat. no. B1558AVIAL; New England Biolabs, Inc.), Wash Buffer II (cat. no. B1559AVIAL; New England Biolabs, Inc.)and Low Salt Buffer (cat. no. B1557AVIAL; New England Biolabs, Inc.).

    Article Title: RNA methyltransferase NSUN2 enhances vasculogenic mimicry and malignant progression of cervical cancer through upregulation of MMP‑9
    Article Snippet: .. Total RNA was incubated with oligo dT(25) beads (cat. no. S1550S; New England Biolabs, Inc.) for 10 min at RT and then washed with Lysis/Binding buffer (cat. no. B1556AVIAL; New England Biolabs, Inc.), Wash Buffer I (cat. no. B1558AVIAL; New England Biolabs, Inc.), Wash Buffer II (cat. no. B1559AVIAL; New England Biolabs, Inc.)and Low Salt Buffer (cat. no. B1557AVIAL; New England Biolabs, Inc.). .. The mRNA was eluted with Elution Buffer (cat. no. B1561AVIAL; New England Biolabs, Inc.) and samples containing 300 or 600 ng of mRNA were transferred to nylon membranes.

    Lysis:

    Article Title: RNA methyltransferase NSUN2 enhances vasculogenic mimicry and malignant progression of cervical cancer through upregulation of MMP-9
    Article Snippet: .. Total RNA was incubated with oligo dT(25) beads (cat. no. S1550S; New England Biolabs, Inc.) for 10 min at RT and then washed with Lysis/Binding buffer (cat. no. B1556AVIAL; New England Biolabs, Inc.), Wash Buffer I (cat. no. B1558AVIAL; New England Biolabs, Inc.), Wash Buffer II (cat. no. B1559AVIAL; New England Biolabs, Inc.)and Low Salt Buffer (cat. no. B1557AVIAL; New England Biolabs, Inc.). .. Total RNA was incubated with oligo dT(25) beads (cat. no. S1550S; New England Biolabs, Inc.) for 10 min at RT and then washed with Lysis/Binding buffer (cat. no. B1556AVIAL; New England Biolabs, Inc.), Wash Buffer I (cat. no. B1558AVIAL; New England Biolabs, Inc.), Wash Buffer II (cat. no. B1559AVIAL; New England Biolabs, Inc.)and Low Salt Buffer (cat. no. B1557AVIAL; New England Biolabs, Inc.).

    Article Title: RNA methyltransferase NSUN2 enhances vasculogenic mimicry and malignant progression of cervical cancer through upregulation of MMP‑9
    Article Snippet: .. Total RNA was incubated with oligo dT(25) beads (cat. no. S1550S; New England Biolabs, Inc.) for 10 min at RT and then washed with Lysis/Binding buffer (cat. no. B1556AVIAL; New England Biolabs, Inc.), Wash Buffer I (cat. no. B1558AVIAL; New England Biolabs, Inc.), Wash Buffer II (cat. no. B1559AVIAL; New England Biolabs, Inc.)and Low Salt Buffer (cat. no. B1557AVIAL; New England Biolabs, Inc.). .. The mRNA was eluted with Elution Buffer (cat. no. B1561AVIAL; New England Biolabs, Inc.) and samples containing 300 or 600 ng of mRNA were transferred to nylon membranes.

    Magnetic Beads:

    Article Title: TOR-dependent regulation of the yeast homolog of the juvenile Batten Disease-associated gene CLN3
    Article Snippet: The RNA concentration was measured using a NanoDrop spectrophotometer (ThermoFisher Scientific) at 260 nm. .. Poly-A RNA was isolated from a total of 80 μ g of RNA using oligo d(T)25 magnetic beads (New England Biolabs, 61002), following the protocol and kit purchased from New England Biolabs (S1550). ..

    other:

    Article Title: Hijacking innate immunity to enhance mRNA therapeutics by blocking IFN-P-body-XRN1 axis-mediated degradation.
    Article Snippet: FastPure cell/tissue total RNA isolation kit V2 (RC11201), HiScript II 1st Strand cDNA Synthesis Kit (+gDNA wiper) (R212-01), and Ultra GelRed (GR501-01) were from Vazyme.

    Transformation Assay:

    Article Title: EffectorGeneP: accurate gene annotation in pathogen genomes from infection transcriptomes
    Article Snippet: .. Messenger (m)RNA was extracted from transformed protoplasts after 24 hours using the NEB Magnetic mRNA Isolation Kit (#S1550S). .. Library-specific cDNA synthesis and PCR was carried out on 30 ng mRNA using the Invitrogen SuperScript IV One-Step RT-PCR System with ezDNase kit (Cat. # 12595100) with the forward and reverse primers ZmUbi1_5UTR_F3b (5’GCACACACACACAACCAG-3’) and FS_cDNA_R (5’- TGCTAGATCTCGACAGTACG-3’) as previously described .

    Sequencing:

    Article Title: A massively parallel reporter assay of MECP2 cis-regulatory elements reveals genetic candidates for male-biased autism
    Article Snippet: RNA and DNA were isolated from each replicate using the Qiagen AllPrep kit (Qiagen 80204). .. To prepare sequencing libraries, RNA was first treated with TURBO DNase (Thermo Fisher Scientific AM2239) to remove any remaining DNA, and then polyA-tailed mRNA was isolated with oligo d(T) beads (NEB S1550S). .. 0.7μg of RNA was then reverse-transcribed using SuperScript III RT (Thermo Fisher Scientific 18080093), using the P7-pLSmP-ass16UMI-gfp primer to add unique molecular identifiers to the cDNA.



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    microarray human scleroderma data set  (Biotechnology Information)
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    Biotechnology Information microarray human scleroderma data set
    The IL-13 pathway is activated in SSc and sclGVHD skin. A: Gene expression data for the 491 IL-13–responsive genes identified in human dermal fibroblasts were extracted from the SSc skin data of Milano et al.4 SSc skin biopsy samples are ordered by intrinsic subset and genes are organized by hierarchical clustering. The centroid average of the IL-13–responsive gene signature at maximal induction (12 and 24 hours) is shown to the left of the heat map. Pearson's correlation coefficients between the centroid and individual patient sample are plotted below each array. B: Expression data for the 734 genes reported by Fulkerson et al26 as IL-13–inducible genes in mouse lung were extracted from the sclGVHD <t>microarray</t> data set obtained 2 weeks and 5 weeks after splenocyte transfer (n = 4 per group). Genes and arrays were organized by hierarchical clustering. The relative expression of IL-13–inducible genes (centroid) in lungs is shown to the left of the heat map. C: Representative IL-13 IHC on skin biopsies obtained from an SSc patient and a normal control subject. Arrowheads indicate IL-13+ cells. Original magnification, ×400. D: Blinded quantification of the number of IHC IL-13+ cells per high-power field (×600) in skin biopsies from SSc patients and normal control subjects. SSc, n = 18; control, n = 6; P = 0.0037. E: IL-13 ELISA on the tissue culture supernatants of skin explants from BALB/c Rag2−/− mice that received either syngeneic BALB/c (n = 3) or allogeneic B10.D2 (n = 3) splenocytes 2 weeks earlier (P = 0.002).
    Microarray Human Scleroderma Data Set, supplied by Biotechnology Information, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    The IL-13 pathway is activated in SSc and sclGVHD skin. A: Gene expression data for the 491 IL-13–responsive genes identified in human dermal fibroblasts were extracted from the SSc skin data of Milano et al.4 SSc skin biopsy samples are ordered by intrinsic subset and genes are organized by hierarchical clustering. The centroid average of the IL-13–responsive gene signature at maximal induction (12 and 24 hours) is shown to the left of the heat map. Pearson's correlation coefficients between the centroid and individual patient sample are plotted below each array. B: Expression data for the 734 genes reported by Fulkerson et al26 as IL-13–inducible genes in mouse lung were extracted from the sclGVHD microarray data set obtained 2 weeks and 5 weeks after splenocyte transfer (n = 4 per group). Genes and arrays were organized by hierarchical clustering. The relative expression of IL-13–inducible genes (centroid) in lungs is shown to the left of the heat map. C: Representative IL-13 IHC on skin biopsies obtained from an SSc patient and a normal control subject. Arrowheads indicate IL-13+ cells. Original magnification, ×400. D: Blinded quantification of the number of IHC IL-13+ cells per high-power field (×600) in skin biopsies from SSc patients and normal control subjects. SSc, n = 18; control, n = 6; P = 0.0037. E: IL-13 ELISA on the tissue culture supernatants of skin explants from BALB/c Rag2−/− mice that received either syngeneic BALB/c (n = 3) or allogeneic B10.D2 (n = 3) splenocytes 2 weeks earlier (P = 0.002).

    Journal: The American Journal of Pathology

    Article Title: Interspecies Comparison of Human and Murine Scleroderma Reveals IL-13 and CCL2 as Disease Subset-Specific Targets

    doi: 10.1016/j.ajpath.2011.11.024

    Figure Lengend Snippet: The IL-13 pathway is activated in SSc and sclGVHD skin. A: Gene expression data for the 491 IL-13–responsive genes identified in human dermal fibroblasts were extracted from the SSc skin data of Milano et al.4 SSc skin biopsy samples are ordered by intrinsic subset and genes are organized by hierarchical clustering. The centroid average of the IL-13–responsive gene signature at maximal induction (12 and 24 hours) is shown to the left of the heat map. Pearson's correlation coefficients between the centroid and individual patient sample are plotted below each array. B: Expression data for the 734 genes reported by Fulkerson et al26 as IL-13–inducible genes in mouse lung were extracted from the sclGVHD microarray data set obtained 2 weeks and 5 weeks after splenocyte transfer (n = 4 per group). Genes and arrays were organized by hierarchical clustering. The relative expression of IL-13–inducible genes (centroid) in lungs is shown to the left of the heat map. C: Representative IL-13 IHC on skin biopsies obtained from an SSc patient and a normal control subject. Arrowheads indicate IL-13+ cells. Original magnification, ×400. D: Blinded quantification of the number of IHC IL-13+ cells per high-power field (×600) in skin biopsies from SSc patients and normal control subjects. SSc, n = 18; control, n = 6; P = 0.0037. E: IL-13 ELISA on the tissue culture supernatants of skin explants from BALB/c Rag2−/− mice that received either syngeneic BALB/c (n = 3) or allogeneic B10.D2 (n = 3) splenocytes 2 weeks earlier (P = 0.002).

    Article Snippet: The 75 microarray human scleroderma data set, previously described, 4 is publicly available at the National Center for Biotechnology Information GEO site ( http://www.ncbi.nlm.nih.gov/geo ; accession {"type":"entrez-geo","attrs":{"text":"GSE9285","term_id":"9285"}} GSE9285 ).

    Techniques: Gene Expression, Expressing, Microarray, Control, Enzyme-linked Immunosorbent Assay