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GeneLAB GmbH microarray transcription profiling data
Microarray Transcription Profiling Data, supplied by GeneLAB GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microarray+dataset/microarray+dataset+e+geod+74708/pmc06915713-251-0-12
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Related Articles

Microarray:

Article Title: Designing a Novel Monitoring Approach for the Effects of Space Travel on Astronauts' Health.
Article Snippet: .. To better understand how space travel affects astronauts’ health, we downloaded the microarray dataset (E-GEOD-74708) from the NASA GeneLab open data repository. ..

Article Title: Global transcriptomic analysis of a murine osteocytic cell line subjected to spaceflight
Article Snippet: © 2021 Federation of American Societies for Experimental Biology Yuhei Uda and Jordan M. Spatz contributed equally to this work.. Abbreviations: 3D, three dimensional; ALP, alkaline phosphatase; ANOVA, analysis of variance; APE 1, apurinic/apyrimidinic endonuclease 1; Car9, carbonic anhydrase 9; CRS, commercial resupply services; CSA, Canadian Space Agency; Ct, cycle threshold; DAVID, database for annotation, visualization and integrated discovery; Dkk1, dickkopf WNT signaling pathway inhibitor 1; Dmp1, dentin matrix protein 1; DPBS, Dulbecco's phosphatebuffered saline; dUTP, 2′deoxyuridine5′triphosphate; Egln3, egl9 family hypoxiainducible factor 3; ELISA, enzymelinked immunosorbent assay; Fam162a, family with sequence similarity 162 member A; FC, fold change; FDR, false discovery rate; GFP, green fluorescent protein; GLDS, GeneLab data systems; GSEA, gene set enrichment analysis; HIF1α, Hypoxiainducible factor 1α; HRP, horseradish peroxidase; iNOS, inducible nitric oxide synthase; IPA, ingenuity pathway analysis; ISS, International Space Station; IVT, in vitro transcription; KEGG, Kyoto encyclopedia of genes and genomes; Mepe, matrix extracellular phosphoglycoprotein; MGI, Mouse Genome Informatics; NASA, National Aeronautics and Space Administration; OC, osteocalcin; OPG, osteoprotegerin; OSTEO4, osteoporosis experiments on orbit4; PBS, phosphatebuffered saline; PC, principal component; PCA, principal component analysis; PCR, polymerase chain reaction; PFA, paraformaldehyde; Pfkfb3, 6phosphofructo2kinase/fructose2,6biphosphatase 3; Phex, phosphate regulating endopeptidase homolog Xlinked; RANKL, receptor activator of nuclear factor kappaβ ligand; RMA, robust multiarray average; SAPE, streptavidinRphycoerythrin; SD, standard deviation; Serping1, Serpin family G member 1; STLV, slowturning lateral vessel; SV40, simian virus 40; T1D, type I diabetes; TdT, terminal deoxynucleotidyl transferase; TMB, 3,3′,5,5′tetramethylbenzidine; UDG, uracil DNA glycosylase; UDP, uridine diphosphate.. 1Department of Translational Dental Medicine, Boston University Henry M. Goldman School of Dental Medicine, Boston, MA, USA 2Endocrine Unit, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA 3School of Medicine, University of California San Francisco, San Francisco, CA, USA 4Department of Orthopaedic Surgery, Boston University School of Medicine, Boston, MA, USA 5CALM Technologies Inc., Kingston, ON, Canada

Article Title: Multi-omics analysis of multiple missions to space reveal a theme of lipid dysregulation in mouse liver
Article Snippet: .. Microarray transcription profiling data from STS-135 mouse liver samples, obtained from the GeneLab Dataset-25 (GLDS-25), was preprocessed in R using the Affy package. ..

Article Title: Skeletal muscle gene expression dysregulation in long-term spaceflights and aging is clock-dependent.
Article Snippet: .. Acquisition of publicly available Microarray datasets Using a criterion of “skeletal muscle”, “mammalian tissue”, and “Microarray” in open source repositories (NCBI-GEO/ArrayExpress/ NASA GeneLab), we obtained a total of 485 samples from 10 different datasets. .. Out of 10 microarray datasets, four datasets npj Microgravity (2023) 30 Published in cooperation with the Biodesign Institute at Arizona State University, with the support of NASA were retrieved from healthy humans and six datasets were retrieved from mice.

Article Title: Rapid Transient Transcriptional Adaptation to Hypergravity in Jurkat T Cells Revealed by Comparative Analysis of Microarray and RNA-Seq Data
Article Snippet: .. The analysis of the microarray data set available at GeneLab (GLDS-13) revealed a significant downregulation of ATP6V1A and ATP6V1D after 3 days of microgravity exposure and 1.5 h of T cell activation ( ). .. In contrast, ATP6V1A and D were not significantly altered in the 48 h simulated microgravity study of TK6 lymphoblastoid cells from Chowdhury and colleagues [ ].

Comparison:

Article Title: Global transcriptomic analysis of a murine osteocytic cell line subjected to spaceflight
Article Snippet: © 2021 Federation of American Societies for Experimental Biology Yuhei Uda and Jordan M. Spatz contributed equally to this work.. Abbreviations: 3D, three dimensional; ALP, alkaline phosphatase; ANOVA, analysis of variance; APE 1, apurinic/apyrimidinic endonuclease 1; Car9, carbonic anhydrase 9; CRS, commercial resupply services; CSA, Canadian Space Agency; Ct, cycle threshold; DAVID, database for annotation, visualization and integrated discovery; Dkk1, dickkopf WNT signaling pathway inhibitor 1; Dmp1, dentin matrix protein 1; DPBS, Dulbecco's phosphatebuffered saline; dUTP, 2′deoxyuridine5′triphosphate; Egln3, egl9 family hypoxiainducible factor 3; ELISA, enzymelinked immunosorbent assay; Fam162a, family with sequence similarity 162 member A; FC, fold change; FDR, false discovery rate; GFP, green fluorescent protein; GLDS, GeneLab data systems; GSEA, gene set enrichment analysis; HIF1α, Hypoxiainducible factor 1α; HRP, horseradish peroxidase; iNOS, inducible nitric oxide synthase; IPA, ingenuity pathway analysis; ISS, International Space Station; IVT, in vitro transcription; KEGG, Kyoto encyclopedia of genes and genomes; Mepe, matrix extracellular phosphoglycoprotein; MGI, Mouse Genome Informatics; NASA, National Aeronautics and Space Administration; OC, osteocalcin; OPG, osteoprotegerin; OSTEO4, osteoporosis experiments on orbit4; PBS, phosphatebuffered saline; PC, principal component; PCA, principal component analysis; PCR, polymerase chain reaction; PFA, paraformaldehyde; Pfkfb3, 6phosphofructo2kinase/fructose2,6biphosphatase 3; Phex, phosphate regulating endopeptidase homolog Xlinked; RANKL, receptor activator of nuclear factor kappaβ ligand; RMA, robust multiarray average; SAPE, streptavidinRphycoerythrin; SD, standard deviation; Serping1, Serpin family G member 1; STLV, slowturning lateral vessel; SV40, simian virus 40; T1D, type I diabetes; TdT, terminal deoxynucleotidyl transferase; TMB, 3,3′,5,5′tetramethylbenzidine; UDG, uracil DNA glycosylase; UDP, uridine diphosphate.. 1Department of Translational Dental Medicine, Boston University Henry M. Goldman School of Dental Medicine, Boston, MA, USA 2Endocrine Unit, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA 3School of Medicine, University of California San Francisco, San Francisco, CA, USA 4Department of Orthopaedic Surgery, Boston University School of Medicine, Boston, MA, USA 5CALM Technologies Inc., Kingston, ON, Canada

Derivative Assay:

Article Title: Global transcriptomic analysis of a murine osteocytic cell line subjected to spaceflight
Article Snippet: © 2021 Federation of American Societies for Experimental Biology Yuhei Uda and Jordan M. Spatz contributed equally to this work.. Abbreviations: 3D, three dimensional; ALP, alkaline phosphatase; ANOVA, analysis of variance; APE 1, apurinic/apyrimidinic endonuclease 1; Car9, carbonic anhydrase 9; CRS, commercial resupply services; CSA, Canadian Space Agency; Ct, cycle threshold; DAVID, database for annotation, visualization and integrated discovery; Dkk1, dickkopf WNT signaling pathway inhibitor 1; Dmp1, dentin matrix protein 1; DPBS, Dulbecco's phosphatebuffered saline; dUTP, 2′deoxyuridine5′triphosphate; Egln3, egl9 family hypoxiainducible factor 3; ELISA, enzymelinked immunosorbent assay; Fam162a, family with sequence similarity 162 member A; FC, fold change; FDR, false discovery rate; GFP, green fluorescent protein; GLDS, GeneLab data systems; GSEA, gene set enrichment analysis; HIF1α, Hypoxiainducible factor 1α; HRP, horseradish peroxidase; iNOS, inducible nitric oxide synthase; IPA, ingenuity pathway analysis; ISS, International Space Station; IVT, in vitro transcription; KEGG, Kyoto encyclopedia of genes and genomes; Mepe, matrix extracellular phosphoglycoprotein; MGI, Mouse Genome Informatics; NASA, National Aeronautics and Space Administration; OC, osteocalcin; OPG, osteoprotegerin; OSTEO4, osteoporosis experiments on orbit4; PBS, phosphatebuffered saline; PC, principal component; PCA, principal component analysis; PCR, polymerase chain reaction; PFA, paraformaldehyde; Pfkfb3, 6phosphofructo2kinase/fructose2,6biphosphatase 3; Phex, phosphate regulating endopeptidase homolog Xlinked; RANKL, receptor activator of nuclear factor kappaβ ligand; RMA, robust multiarray average; SAPE, streptavidinRphycoerythrin; SD, standard deviation; Serping1, Serpin family G member 1; STLV, slowturning lateral vessel; SV40, simian virus 40; T1D, type I diabetes; TdT, terminal deoxynucleotidyl transferase; TMB, 3,3′,5,5′tetramethylbenzidine; UDG, uracil DNA glycosylase; UDP, uridine diphosphate.. 1Department of Translational Dental Medicine, Boston University Henry M. Goldman School of Dental Medicine, Boston, MA, USA 2Endocrine Unit, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA 3School of Medicine, University of California San Francisco, San Francisco, CA, USA 4Department of Orthopaedic Surgery, Boston University School of Medicine, Boston, MA, USA 5CALM Technologies Inc., Kingston, ON, Canada

Isolation:

Article Title: Global transcriptomic analysis of a murine osteocytic cell line subjected to spaceflight
Article Snippet: © 2021 Federation of American Societies for Experimental Biology Yuhei Uda and Jordan M. Spatz contributed equally to this work.. Abbreviations: 3D, three dimensional; ALP, alkaline phosphatase; ANOVA, analysis of variance; APE 1, apurinic/apyrimidinic endonuclease 1; Car9, carbonic anhydrase 9; CRS, commercial resupply services; CSA, Canadian Space Agency; Ct, cycle threshold; DAVID, database for annotation, visualization and integrated discovery; Dkk1, dickkopf WNT signaling pathway inhibitor 1; Dmp1, dentin matrix protein 1; DPBS, Dulbecco's phosphatebuffered saline; dUTP, 2′deoxyuridine5′triphosphate; Egln3, egl9 family hypoxiainducible factor 3; ELISA, enzymelinked immunosorbent assay; Fam162a, family with sequence similarity 162 member A; FC, fold change; FDR, false discovery rate; GFP, green fluorescent protein; GLDS, GeneLab data systems; GSEA, gene set enrichment analysis; HIF1α, Hypoxiainducible factor 1α; HRP, horseradish peroxidase; iNOS, inducible nitric oxide synthase; IPA, ingenuity pathway analysis; ISS, International Space Station; IVT, in vitro transcription; KEGG, Kyoto encyclopedia of genes and genomes; Mepe, matrix extracellular phosphoglycoprotein; MGI, Mouse Genome Informatics; NASA, National Aeronautics and Space Administration; OC, osteocalcin; OPG, osteoprotegerin; OSTEO4, osteoporosis experiments on orbit4; PBS, phosphatebuffered saline; PC, principal component; PCA, principal component analysis; PCR, polymerase chain reaction; PFA, paraformaldehyde; Pfkfb3, 6phosphofructo2kinase/fructose2,6biphosphatase 3; Phex, phosphate regulating endopeptidase homolog Xlinked; RANKL, receptor activator of nuclear factor kappaβ ligand; RMA, robust multiarray average; SAPE, streptavidinRphycoerythrin; SD, standard deviation; Serping1, Serpin family G member 1; STLV, slowturning lateral vessel; SV40, simian virus 40; T1D, type I diabetes; TdT, terminal deoxynucleotidyl transferase; TMB, 3,3′,5,5′tetramethylbenzidine; UDG, uracil DNA glycosylase; UDP, uridine diphosphate.. 1Department of Translational Dental Medicine, Boston University Henry M. Goldman School of Dental Medicine, Boston, MA, USA 2Endocrine Unit, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA 3School of Medicine, University of California San Francisco, San Francisco, CA, USA 4Department of Orthopaedic Surgery, Boston University School of Medicine, Boston, MA, USA 5CALM Technologies Inc., Kingston, ON, Canada

other:

Article Title: Designing a Novel Monitoring Approach for the Effects of Space Travel on Astronauts' Health.
Article Snippet: Microarray dataset E-GEOD-74708, which is the largest publicly available space gene expression dataset, was downloaded from the NASA GeneLab Database (https://genelabdata.ndc.nasa.gov/genelab, accessed on 15 March 2021).

Article Title: Designing a Novel Monitoring Approach for the Effects of Space Travel on Astronauts’ Health
Article Snippet: Microarray dataset E-GEOD-74708, which is the largest publicly available space gene expression dataset, was downloaded from the NASA GeneLab Database ( https://genelab-data.ndc.nasa.gov/genelab , accessed on 15 March 2021).

Activation Assay:

Article Title: Rapid Transient Transcriptional Adaptation to Hypergravity in Jurkat T Cells Revealed by Comparative Analysis of Microarray and RNA-Seq Data
Article Snippet: .. The analysis of the microarray data set available at GeneLab (GLDS-13) revealed a significant downregulation of ATP6V1A and ATP6V1D after 3 days of microgravity exposure and 1.5 h of T cell activation ( ). .. In contrast, ATP6V1A and D were not significantly altered in the 48 h simulated microgravity study of TK6 lymphoblastoid cells from Chowdhury and colleagues [ ].



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Image Search Results


TRIM21 is elevated in astrocytes across MS. A Analysis of TRIM21 mRNA expression in the control and white matter lesion tissues of MS patients based on gene expression profiling microarray data ( GSE138614 ). B TRIM21 mRNA levels in PBMC samples of healthy controls (n = 12) and MS patients (n = 20) were quantified by RT-qPCR. C Representative immunoblots and corresponding quantification of TRIM21 protein levels in brain tissues from EAE mice at 28 dpi (n = 5). D Representative immunoblot images and the corresponding quantitative analysis of TRIM21 protein expression in spinal cord tissues from EAE mice at 28 dpi (n = 5). E Immunofluorescence co-localization analysis of TRIM21 (red) and the astrocyte marker GFAP (green) in brain and spinal cord sections from control and EAE mice at 28 dpi (n = 5). Scale bar, 50 µm

Journal: Journal of Neuroinflammation

Article Title: TRIM21 promotes astrocyte-mediated neuroinflammation in experimental autoimmune encephalomyelitis by stabilizing RGMa via K33-linked ubiquitination

doi: 10.1186/s12974-026-03769-4

Figure Lengend Snippet: TRIM21 is elevated in astrocytes across MS. A Analysis of TRIM21 mRNA expression in the control and white matter lesion tissues of MS patients based on gene expression profiling microarray data ( GSE138614 ). B TRIM21 mRNA levels in PBMC samples of healthy controls (n = 12) and MS patients (n = 20) were quantified by RT-qPCR. C Representative immunoblots and corresponding quantification of TRIM21 protein levels in brain tissues from EAE mice at 28 dpi (n = 5). D Representative immunoblot images and the corresponding quantitative analysis of TRIM21 protein expression in spinal cord tissues from EAE mice at 28 dpi (n = 5). E Immunofluorescence co-localization analysis of TRIM21 (red) and the astrocyte marker GFAP (green) in brain and spinal cord sections from control and EAE mice at 28 dpi (n = 5). Scale bar, 50 µm

Article Snippet: The microarray dataset GSE138614 was retrieved from the Gene Expression Omnibus (GEO) repository of the National Center for Biotechnology Information ( https://www.ncbi.nlm.nih.gov/geo/ ).

Techniques: Expressing, Control, Gene Expression, Microarray, Quantitative RT-PCR, Western Blot, Immunofluorescence, Marker

SIRT3 is downregulated in RCC. ( A ) Based on the GEO GSE53757 dataset, SIRT3 mRNA levels in RCC tissues were compared with those in normal tissues. ( B ) SIRT3 protein levels in RCC tissues were analyzed in comparison to adjacent normal tissues using CPTAC data. ( C ) SIRT3 protein levels in RCC tissues and normal tissues were detected using immunohistochemistry (IHC). A scatter plot was generated to display the expression of SIRT3 in adjacent normal tissues versus RCC tissues. ( D, E ) Enrichment plots were produced to illustrate the gene expression signatures for proliferation (CHIANG_LIVER_CANCER_SUBCLASS_PROLIFERATION_DN) and migration (GOBP_ENDOTHELIAL_CELL_MIGRATION). *** P < 0.001.

Journal: Scientific Reports

Article Title: SIRT3 suppresses renal cancer progression by regulating IDH2 acetylation

doi: 10.1038/s41598-026-37783-6

Figure Lengend Snippet: SIRT3 is downregulated in RCC. ( A ) Based on the GEO GSE53757 dataset, SIRT3 mRNA levels in RCC tissues were compared with those in normal tissues. ( B ) SIRT3 protein levels in RCC tissues were analyzed in comparison to adjacent normal tissues using CPTAC data. ( C ) SIRT3 protein levels in RCC tissues and normal tissues were detected using immunohistochemistry (IHC). A scatter plot was generated to display the expression of SIRT3 in adjacent normal tissues versus RCC tissues. ( D, E ) Enrichment plots were produced to illustrate the gene expression signatures for proliferation (CHIANG_LIVER_CANCER_SUBCLASS_PROLIFERATION_DN) and migration (GOBP_ENDOTHELIAL_CELL_MIGRATION). *** P < 0.001.

Article Snippet: The GSE53757 microarray dataset was obtained through the National Center for Biotechnology Information Gene Expression Omnibus database (NCBI GEO, https://www.ncbi.nlm.nih.gov/gds/?term=GSE53757 ).

Techniques: Comparison, Immunohistochemistry, Generated, Expressing, Produced, Gene Expression, Migration