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microarray hybridization chamber  (Corning Life Sciences)

 
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    Corning Life Sciences microarray hybridization chamber
    Plot of gene expression of sorted/unsorted cells . Plot of one-sample T-test p-values with fold-change in gene expression for all ORFs in <t>microarray</t> study I. Vertical lines show the cutoff of fold-change of 2 (Log 2 ratio of ± 1), while the horizontal line shows the cutoff of p-value 0.05. Genes located in the left-bottom corner (Log 2 ratio <-1 and p-value <0.05) and in the right-bottom corner (Log 2 ratio >1 and p-value <0.05) were considered to have their expressions changed due to dispersion/homogenization and IMS (immuno-magnetic separation) cell sorting. A total of ten genes were selected using these criteria, eight of which also differentially expressed in the independent microarray study II.
    Microarray Hybridization Chamber, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microarray+chambers/pmc03076228-154-4-8?v=Corning+Life+Sciences
    Average 90 stars, based on 1 article reviews
    microarray hybridization chamber - by Bioz Stars, 2026-07
    90/100 stars

    Images

    1) Product Images from "Separation of the bacterial species, Escherichia coli , from mixed-species microbial communities for transcriptome analysis"

    Article Title: Separation of the bacterial species, Escherichia coli , from mixed-species microbial communities for transcriptome analysis

    Journal: BMC Microbiology

    doi: 10.1186/1471-2180-11-59

    Plot of gene expression of sorted/unsorted cells . Plot of one-sample T-test p-values with fold-change in gene expression for all ORFs in microarray study I. Vertical lines show the cutoff of fold-change of 2 (Log 2 ratio of ± 1), while the horizontal line shows the cutoff of p-value 0.05. Genes located in the left-bottom corner (Log 2 ratio <-1 and p-value <0.05) and in the right-bottom corner (Log 2 ratio >1 and p-value <0.05) were considered to have their expressions changed due to dispersion/homogenization and IMS (immuno-magnetic separation) cell sorting. A total of ten genes were selected using these criteria, eight of which also differentially expressed in the independent microarray study II.
    Figure Legend Snippet: Plot of gene expression of sorted/unsorted cells . Plot of one-sample T-test p-values with fold-change in gene expression for all ORFs in microarray study I. Vertical lines show the cutoff of fold-change of 2 (Log 2 ratio of ± 1), while the horizontal line shows the cutoff of p-value 0.05. Genes located in the left-bottom corner (Log 2 ratio <-1 and p-value <0.05) and in the right-bottom corner (Log 2 ratio >1 and p-value <0.05) were considered to have their expressions changed due to dispersion/homogenization and IMS (immuno-magnetic separation) cell sorting. A total of ten genes were selected using these criteria, eight of which also differentially expressed in the independent microarray study II.

    Techniques Used: Expressing, Microarray, Homogenization, FACS

    Genes identified as differentially expressed # between IMS sorted E. coli cells versus unsorted E. coli cells* by the method of cDNA microarray and their differential expression confirmed with another method of qPCR
    Figure Legend Snippet: Genes identified as differentially expressed # between IMS sorted E. coli cells versus unsorted E. coli cells* by the method of cDNA microarray and their differential expression confirmed with another method of qPCR

    Techniques Used: Microarray, Expressing



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    Mature miR- 221 is overexpressed in AML. 22 blast-enriched AML samples and 9 healthy controls (3 CD34+, 3 BM, 3 PB), all of which had also been included in the <t>microarray</t> experiments, were subjected to Taqman qRT-PCR to measure mature miR-221 (A) or miR-222 (B) . Expression values were calculated according to the ΔΔCt method , with RNU6B as internal reference and PB from healthy donor A as calibrator. *, p < 0.05; n.s., not significant (miR-222, p = 0.064).
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    Image Search Results


    Mature miR- 221 is overexpressed in AML. 22 blast-enriched AML samples and 9 healthy controls (3 CD34+, 3 BM, 3 PB), all of which had also been included in the microarray experiments, were subjected to Taqman qRT-PCR to measure mature miR-221 (A) or miR-222 (B) . Expression values were calculated according to the ΔΔCt method , with RNU6B as internal reference and PB from healthy donor A as calibrator. *, p < 0.05; n.s., not significant (miR-222, p = 0.064).

    Journal: BMC Cancer

    Article Title: Overexpression of primary microRNA 221/222 in acute myeloid leukemia

    doi: 10.1186/1471-2407-13-364

    Figure Lengend Snippet: Mature miR- 221 is overexpressed in AML. 22 blast-enriched AML samples and 9 healthy controls (3 CD34+, 3 BM, 3 PB), all of which had also been included in the microarray experiments, were subjected to Taqman qRT-PCR to measure mature miR-221 (A) or miR-222 (B) . Expression values were calculated according to the ΔΔCt method , with RNU6B as internal reference and PB from healthy donor A as calibrator. *, p < 0.05; n.s., not significant (miR-222, p = 0.064).

    Article Snippet: Arrays were hybridized overnight at 60°C in microarray hybridization chambers (Corning, Corning, NY, USA).

    Techniques: Microarray, Quantitative RT-PCR, Expressing