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LC Sciences micrornas microarray profiling
Role of miR-200a in the control of St3gal3 and St3gal4 in murine primary hepatocytes. ( A ) Fold increase of miR-200a in neonate vs . adult liver was determined in a <t>miRNA</t> array (chip 1 and chip 2) and validated by qRT-PCR in livers from 5 adult and 14 neonate mice. ( B ) Primary hepatocytes from adult mice were transfected with precursor molecules for miR-200a and miR-17-3p and with a scrambled oligonucleotide at 100 nmol/L. After 48 h, total RNA was purified and levels of St3gal3 and St3gal4 were measured by qRT-PCR and normalized with respect to β-actin mRNA. The normalized data were expressed as changes relative to the data of the cells transfected with scrambled pre-miR and set as 100%. Differences were analyzed by means of Student's t test. Statistical significance was taken as *p<0.05, **p<0.01, NS: not statistically significant. The data shown are expressed as mean ± SD, representative of four independent experiments with three replicates each.
Micrornas Microarray Profiling, supplied by LC Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microarray+based+profiling+of+micrornas/micrornas+microarray+profiling/pmc03674942-40-0-26
Average 90 stars, based on 1 article reviews
micrornas microarray profiling - by Bioz Stars, 2026-09
90/100 stars

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1) Product Images from "Control of post-translational modifications in antithrombin during murine post-natal development by miR-200a"

Article Title: Control of post-translational modifications in antithrombin during murine post-natal development by miR-200a

Journal: Journal of Biomedical Science

doi: 10.1186/1423-0127-20-29

Role of miR-200a in the control of St3gal3 and St3gal4 in murine primary hepatocytes. ( A ) Fold increase of miR-200a in neonate vs . adult liver was determined in a miRNA array (chip 1 and chip 2) and validated by qRT-PCR in livers from 5 adult and 14 neonate mice. ( B ) Primary hepatocytes from adult mice were transfected with precursor molecules for miR-200a and miR-17-3p and with a scrambled oligonucleotide at 100 nmol/L. After 48 h, total RNA was purified and levels of St3gal3 and St3gal4 were measured by qRT-PCR and normalized with respect to β-actin mRNA. The normalized data were expressed as changes relative to the data of the cells transfected with scrambled pre-miR and set as 100%. Differences were analyzed by means of Student's t test. Statistical significance was taken as *p<0.05, **p<0.01, NS: not statistically significant. The data shown are expressed as mean ± SD, representative of four independent experiments with three replicates each.
Figure Legend Snippet: Role of miR-200a in the control of St3gal3 and St3gal4 in murine primary hepatocytes. ( A ) Fold increase of miR-200a in neonate vs . adult liver was determined in a miRNA array (chip 1 and chip 2) and validated by qRT-PCR in livers from 5 adult and 14 neonate mice. ( B ) Primary hepatocytes from adult mice were transfected with precursor molecules for miR-200a and miR-17-3p and with a scrambled oligonucleotide at 100 nmol/L. After 48 h, total RNA was purified and levels of St3gal3 and St3gal4 were measured by qRT-PCR and normalized with respect to β-actin mRNA. The normalized data were expressed as changes relative to the data of the cells transfected with scrambled pre-miR and set as 100%. Differences were analyzed by means of Student's t test. Statistical significance was taken as *p<0.05, **p<0.01, NS: not statistically significant. The data shown are expressed as mean ± SD, representative of four independent experiments with three replicates each.

Techniques Used: Control, Quantitative RT-PCR, Transfection, Purification

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Article Title: Control of post-translational modifications in antithrombin during murine post-natal development by miR-200a
Article Snippet: The RNA concentration and 260/280 ratio were determined by using NanoDrop spectrophotometer (Thermo Scientific, Wilmington, DE) and RNA integrity was verified by lab-on-chip technology using the Experion automated electrophoresis system (Bio-Rad Laboratories, Madrid, Spain). .. MicroRNAs microarray profiling was performed using total RNA extracted from the liver from one adult mouse (day +50) and one neonate mouse (day +1) using the LC Sciences technology (LC Sciences, Houston, TX). ..



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