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human circrna array v2 microarray analysis (CapitalBio Corporation)

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CapitalBio Corporation human circrna array v2 microarray analysis
Human Circrna Array V2 Microarray Analysis, supplied by CapitalBio Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microarray+analysis+of+human+circrnas/human+circrna+array+v2/pm38925846-64-38-44
Average 90 stars, based on 1 article reviews

human circrna array v2 microarray analysis - by Bioz Stars, 2026-11

90/100 stars

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Purification:

Article Title: Comprehensive Analysis of Circular RNA Expression Profiles in Gefitinib-Resistant Lung Adenocarcinoma Patients.
Article Snippet: RNA was digested, amplified, and labeled by the cRNA Amplification and Labeling Kit (CapitalBio, Beijing, China) according to the manufacturer’s product instruction. .. The purified RNA was hybridized with the CapitalBio Technology Human CircRNA Array v2 which was designed with 4 identical arrays per slide (4× 180 K format) and each array containing probes interrogating about 170,340 human circRNAs. ..

Microarray:

Article Title: Clinical diagnostic value of circ-ARHGER28 for breast cancer and its effect on MCF-7 cell proliferation and apoptosis
Article Snippet: .. The NCBIPrimer database was used to design speci c primers for target genes, and the sequences of the speci c primers are listed in Table S1. circRNA microarray data analysis. circRNA microarray expression pro ling was performed via Human CircRNA Array v2 microarray analysis (CapitalBio Corporation). ..

Article Title: Clinical Diagnostic Value of circ-ARHGER28 for Breast Cancer and its Effect on MCF 7 Cell Proliferation and Apoptosis.
Article Snippet: .. The NCBIPrimer database was used to design speci c primers for target genes, and the sequences of the speci c primers are listed in Table S1. circRNA microarray data analysis. circRNA microarray expression pro ling was performed via Human CircRNA Array v2 microarray analysis (CapitalBio Corporation). ..

Expressing:

Article Title: Recent advances in exosomal RNAs analysis towards diagnostic and therapeutic applications
Article Snippet: Exosomes, small extracellular vesicles (EVs) with sizes of 30e150 nm, are formed by an endosomal route and play a series of important roles in many physiological and pathological processes.. Among all exosomal cargos, various RNAs selectively enclosed into exosomes have been recognized as mediators of intercellular communications and promising tools in clinical diagnosis and therapy.. Accurate and sensitive exosomal RNAs analysis is crucial for both basic research and clinical applications.

Article Title: The novel biomarker circ_0020339 drives septic acute kidney injury by targeting miR-17-5p/IPMK axis.
Article Snippet: Purpose Sepsis is a systemic life-threatening inflammatory disease, which leads to septic acute kidney injury (AKI).. Circular RNAs (circRNAs) are involved in septic AKI.. Herein, we aimed to expound the action of circ_0020339 in septic AKI.

Article Title: Identification of Plasma hsa_circ_0001230 and hsa_circ_0023879 as Potential Novel Biomarkers for Focal Segmental Glomerulosclerosis and circRNA-miRNA-mRNA Network Analysis.
Article Snippet: .. CircRNA Expression Profile Analysis The plasma circRNA of 3 patients with FSGS and three normal controls were analyzed using Human CircRNA Array v2 (CapitalBio, Beijing, China). ..

other:

Article Title: Circular RNA CCT3 is a unique molecular marker in bladder cancer
Article Snippet: Human CircRNA Array v2.1 (CapitalBio Technology, China) performed CircRNA microarray analysis.

Article Title: Construction of a ceRNA regulatory network to explore potential pathogenesis mechanisms involved in human hepatocellular carcinoma.
Article Snippet: The CapitalBio Human CircRNA Array v2.1 was designed with 4 identical arrays per slide (4 × 180 K format), detects human gene expression, using AG-GE-WL11-01-2010 and AG-GEDL00-01-2010.

Clinical Proteomics:

Image Search Results

A novel up-regulated circRNA circ-ANXA7 is an independent prognostic factor for LUAD. a circ-ANXA7 up-regulation was found between LUAD tissues (n = 40) and non-tumor tissues (n = 40) by microarray analysis. Red: up-regulation and green: down-regulation. b Box plots visualizing a higher expression level of circ-ANXA7 in LUAD tissues than non-tumor tissues using qRT-PCR. c qRT-PCR was utilized to examine the relative expression levels of circ-ANXA7 between normal epithelial cells and LUAD cells. d Overall survival analysis between LUAD patients with high circ-ANXA7 expression and those with its low expression. e Multivariate regression analysis of circ-ANXA7 expression after adjusting other prognostic factors. f Western blot was presented to examine the expression of ANXA7 protein between normal epithelial cells and LUAD cells. g Immunohistochemistry of ANXA7 between adjacent normal tissues and LUAD tissues. Magnification: ×40; ×200. **p < 0.01

Journal: Cancer Cell International

Article Title: circ-ANXA7 facilitates lung adenocarcinoma progression via miR-331/LAD1 axis

doi: 10.1186/s12935-021-01791-5

Figure Lengend Snippet: A novel up-regulated circRNA circ-ANXA7 is an independent prognostic factor for LUAD. a circ-ANXA7 up-regulation was found between LUAD tissues (n = 40) and non-tumor tissues (n = 40) by microarray analysis. Red: up-regulation and green: down-regulation. b Box plots visualizing a higher expression level of circ-ANXA7 in LUAD tissues than non-tumor tissues using qRT-PCR. c qRT-PCR was utilized to examine the relative expression levels of circ-ANXA7 between normal epithelial cells and LUAD cells. d Overall survival analysis between LUAD patients with high circ-ANXA7 expression and those with its low expression. e Multivariate regression analysis of circ-ANXA7 expression after adjusting other prognostic factors. f Western blot was presented to examine the expression of ANXA7 protein between normal epithelial cells and LUAD cells. g Immunohistochemistry of ANXA7 between adjacent normal tissues and LUAD tissues. Magnification: ×40; ×200. **p < 0.01

Article Snippet: Arraystar Human circRNA Microarray analysis (Arraystar Inc., Rockville, MD, USA) was then presented.

Techniques: Microarray, Expressing, Quantitative RT-PCR, Western Blot, Immunohistochemistry

circ-ANXA7 could directly target the expression of miR-331. a A schematic diagram showing the putative binding sites between circ-ANXA7 and miR-331. b MiRNA microarray expression profiles identified down-regulated miR-331 between LUAD tumor tissues and non-tumor tissues, which was verified by RT-qPCR. c Down-regulated miR-331 was determined in LUAD cells compared to controls by RT-qPCR. d RT-qPCR was utilized to examine miR-331 expression in A549 cells under transfection of pcDNA3.1-circ-ANXA7 and/or miR-331 mimics. e miR-331 expression was determined in A549 cells transfected with sh-circ-ANXA7 and/or miR-331 inhibitor. f Dual luciferase report between circ-ANXA7 and miR-331. g Correlation between circ-ANXA7 and miR-331. **p < 0.01

Journal: Cancer Cell International

Article Title: circ-ANXA7 facilitates lung adenocarcinoma progression via miR-331/LAD1 axis

doi: 10.1186/s12935-021-01791-5

Figure Lengend Snippet: circ-ANXA7 could directly target the expression of miR-331. a A schematic diagram showing the putative binding sites between circ-ANXA7 and miR-331. b MiRNA microarray expression profiles identified down-regulated miR-331 between LUAD tumor tissues and non-tumor tissues, which was verified by RT-qPCR. c Down-regulated miR-331 was determined in LUAD cells compared to controls by RT-qPCR. d RT-qPCR was utilized to examine miR-331 expression in A549 cells under transfection of pcDNA3.1-circ-ANXA7 and/or miR-331 mimics. e miR-331 expression was determined in A549 cells transfected with sh-circ-ANXA7 and/or miR-331 inhibitor. f Dual luciferase report between circ-ANXA7 and miR-331. g Correlation between circ-ANXA7 and miR-331. **p < 0.01

Article Snippet: Arraystar Human circRNA Microarray analysis (Arraystar Inc., Rockville, MD, USA) was then presented.

Techniques: Expressing, Binding Assay, Microarray, Quantitative RT-PCR, Transfection, Luciferase

a Volcano plots showed differential expression of circRNAs detected by circRNA microarray in IVDD compared with the control. b Volcano plots showed differential expression of circRNAs in GSE67566. c The 9 downregulated circRNAs in IVDD were identified based on the overlap of circRNA microarray and GSE67566. d Heatmap of 9 circRNAs in circRNA microarray and heatmap of 9 circRNAs in GSE67566. e qRT-PCR analysis confirmed the downregulation of circRNAs in IVDD compared with control. * p < 0.05. f circ ERCC2 is transcribed from 13, 14, and 15 exons of the ERCC2 gene. The expression of circ ERCC2 was lower in NP tissues from IVDD compared with the control detected by FISH. g FISH detection of circ ERCC2 in the cytoplasm of NPCs. In ( f ) and ( g ), blue fluorescence indicated the nucleus and green fluorescence indicated circ ERCC2. Scale bar: 20 μm. h Representative plots of apoptosis detected by flow cytometry. circ ERCC2 inhibited the rate of apoptosis of NPCs. * p < 0.05, ** p < 0.01. i NPCs were treated by TBHP or/and circ ERCC2, and mitophagy and apoptosis related proteins were detected by Western blot analysis

Journal: Cell Death & Disease

Article Title: Circ ERCC2 ameliorated intervertebral disc degeneration by regulating mitophagy and apoptosis through miR-182-5p/SIRT1 axis

doi: 10.1038/s41419-019-1978-2

Figure Lengend Snippet: a Volcano plots showed differential expression of circRNAs detected by circRNA microarray in IVDD compared with the control. b Volcano plots showed differential expression of circRNAs in GSE67566. c The 9 downregulated circRNAs in IVDD were identified based on the overlap of circRNA microarray and GSE67566. d Heatmap of 9 circRNAs in circRNA microarray and heatmap of 9 circRNAs in GSE67566. e qRT-PCR analysis confirmed the downregulation of circRNAs in IVDD compared with control. * p < 0.05. f circ ERCC2 is transcribed from 13, 14, and 15 exons of the ERCC2 gene. The expression of circ ERCC2 was lower in NP tissues from IVDD compared with the control detected by FISH. g FISH detection of circ ERCC2 in the cytoplasm of NPCs. In ( f ) and ( g ), blue fluorescence indicated the nucleus and green fluorescence indicated circ ERCC2. Scale bar: 20 μm. h Representative plots of apoptosis detected by flow cytometry. circ ERCC2 inhibited the rate of apoptosis of NPCs. * p < 0.05, ** p < 0.01. i NPCs were treated by TBHP or/and circ ERCC2, and mitophagy and apoptosis related proteins were detected by Western blot analysis

Article Snippet: Identification of differentially expressed circRNAs was performed by overlapping microarray analysis of human circRNAs (Arraystar, CA, USA) and microarray dataset (GSE67566) obtained from Gene Expression Omnibus (GEO) database.

Techniques: Quantitative Proteomics, Microarray, Control, Quantitative RT-PCR, Expressing, Fluorescence, Flow Cytometry, Western Blot