expression microarray (Incyte corporation)

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Incyte corporation expression microarray

Overview of the production and use of expression microarrays. 3′ Expression arrays use synthetically derived oligo probes with design based on mRNA Databases (RefSeq mRNAs, GenBank mRNAs, and ESTs from dbEST) or cDNA derived from bacterial libraries (see ). Sample mRNA can be labeled using two methods (a) Cy3/Cy5 labeling: sample mRNA is reverse transcribed into cDNA and Cy3 is added to one sample and Cy5 to another. Both labeled samples are hybridized to the same <t>microarray.</t> (b) 3′ IVT array: sample mRNA is reverse transcribed to cDNA using oligo(dT) primers, to provide a template for transcription. Using biotin-conjugated nucleotides, the template cDNA is then converted to amplified RNA (aRNA). The biotin-labeled aRNA samples are then fragmented and hybridized onto 3′ expression arrays. A biotin binding fluorescent stain is added to the microarray after hybridization. (c) Affymetrix HuExon 1.0 ST: sample mRNA is reverse transcribed to cDNA using random primers, to provide a template for transcription. The resulting RNA is then reverse transcribed in the presence of dUTPs which are incorporated occasionally into the cDNA sequence instead of dTTP. An enzyme is then used to cleave the cDNA at the site of dUTP incorporation and fragments are terminally labeled before hybridization onto the array. The microarray is then washed and stained after hybridization.

Expression Microarray, supplied by Incyte corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microarray+(gene+expression+profiling)/gene+expression+microarrays++gem+/pmc03303677-133-33-48
Average 90 stars, based on 1 article reviews

expression microarray - by Bioz Stars, 2026-09

90/100 stars

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1) Product Images from "How Can Microarrays Unlock Asthma?"

Article Title: How Can Microarrays Unlock Asthma?

Journal: Journal of Allergy

doi: 10.1155/2012/241314

Figure Legend Snippet: Overview of the production and use of expression microarrays. 3′ Expression arrays use synthetically derived oligo probes with design based on mRNA Databases (RefSeq mRNAs, GenBank mRNAs, and ESTs from dbEST) or cDNA derived from bacterial libraries (see ). Sample mRNA can be labeled using two methods (a) Cy3/Cy5 labeling: sample mRNA is reverse transcribed into cDNA and Cy3 is added to one sample and Cy5 to another. Both labeled samples are hybridized to the same microarray. (b) 3′ IVT array: sample mRNA is reverse transcribed to cDNA using oligo(dT) primers, to provide a template for transcription. Using biotin-conjugated nucleotides, the template cDNA is then converted to amplified RNA (aRNA). The biotin-labeled aRNA samples are then fragmented and hybridized onto 3′ expression arrays. A biotin binding fluorescent stain is added to the microarray after hybridization. (c) Affymetrix HuExon 1.0 ST: sample mRNA is reverse transcribed to cDNA using random primers, to provide a template for transcription. The resulting RNA is then reverse transcribed in the presence of dUTPs which are incorporated occasionally into the cDNA sequence instead of dTTP. An enzyme is then used to cleave the cDNA at the site of dUTP incorporation and fragments are terminally labeled before hybridization onto the array. The microarray is then washed and stained after hybridization.

Techniques Used: Expressing, Derivative Assay, Labeling, Reverse Transcription, Microarray, Amplification, Binding Assay, Staining, Hybridization, Sequencing

Figure Legend Snippet: The GEO accession number for microarray studies conducted on asthma.

Techniques Used: Microarray, Gene Expression, Clone Assay, Expressing, Virus, Infection, Functional Assay, Sequencing, Synthesized, Comparison

Expressing:

Article Title: Regulation of c-Met-dependent gene expression by PTEN.
Article Snippet: .. SF/HGF alters the expression of many more genes in glioblastoma cells than in normal astrocytes We used Incyte gene microarrays to compare the effects of SF/HGF on gene expression profiles in NHA and U-373MG human glioblastoma cells. ..

Article Title: How Can Microarrays Unlock Asthma?
Article Snippet: .. In an attempt to identify the role of IL13 and its isotypes in the pathogenesis of allergic asthma, Syed et al. looked at the effect of IL13 and IL13R130Q on ASM using an expression microarray containing 8159 human gene cDNA clones from Research Genetics (IMAGE consortium, Huntsville, AL), Incyte Genomics (Santa Clara, CA) [ ]. ..

Gene Expression:

Article Title: Identification and function of hypoxia-response genes in Drosophila melanogaster.
Article Snippet: .. A gene expression microarray with 21,000 spots ( 16,000 spots from PCRs of predicted open reading frames from Incyte Genomics and 5,000 from DGC cDNA clones from BDGP) were printed on poly-L-lysine-coated slides and after postprocessing were used for hybridization as in Ref. 1. ..

Article Title: Targeting Tomoregulin for Radioimmunotherapy of Prostate Cancer
Article Snippet: Radiotherapy is an effective approach for the treatment of local prostate cancer.. However, once prostate cancer metastasizes, radiotherapy cannot be used due to the distribution of multiple metastases to lymph nodes and bones.. In contrast, radioimmunotherapy should still be efficacious in metastatic prostate cancer as radioisotopes are brought to tumor cells by targeting antibodies.

Article Title: SC6 for diagnosis of cancers
Article Snippet: .. Gene Expression Microarray (GEM) Analysis A detailed analysis of differential gene expression in HDMEC cells grown under normoxic (control) or hypoxic (treated) conditions for 4, 8 and 16 h was performed using the GEMTM microarray services offered by Incyte Genomics, Ltd, Paloaoto, USA). ..

Microarray:

Article Title: Progressions on the Coexistence of Neuronal and Glial Precursor Cells in the Cerebral Ventricular Zone
Article Snippet: .. When I moved to the University of Pittsburgh, I had already been considering the new gene microarray technologies that were early in production by Affimetrix and Incyte for application to our developmental studies of molecular heterogeneity. ..

Clone Assay:

Hybridization:

other:

Article Title: Fluorescent oligonucleotides and uses thereof
Article Snippet: Microarray-based Gene Expression Assays Custom Gene Expression Microarrays (GEMs) with approximately 10000 elements (cDNA clones from either corn or soybean) were fabricated and provided by Incyte Microarray Division (Fremont.

Article Title: Transcriptional analysis and functional genomics in wheat
Article Snippet: In the post-genomic era the development of tools such as transcription microarrays allows the investigation of the molecular basis and regulation of plant growth, development and physiology in ever increasing detail.. Systems such as the ‘model’ plant species Arabidopsis offer the opportunity to develop generalised models for complex genetic regulatory networks.. However, researchers will need to apply tools to both monitor and alter gene expression in crop plants such as wheat to produce sophisticated and predictive models of development and environmental interactions.

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