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3d model visualization software  (Bruker Corporation)


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    Bruker Corporation 3d model visualization software
    Fig. 2 GNPs treatment alleviated ACLT-induced OA. (A) To evaluate the mitigation of GNPs on OA, C57BL/6J mice (6–8 weeks, male, 20–25 g) were selected to undergo anterior cruciate ligament transection surgery to induce the OA models, and randomly assigned to ACLTVehicle or ACLTGNPs group. The ACLTVehicle and ACLTGNPs groups were given continuous gavage with the equivalent amount of saline and GNPs for 8 weeks, respectively. (B) H&E, Safranine O and Toluidine Blue staining of femur articular cartilage in ACLTVehicle and ACLTGNPs groups. (C) Immunohistochemical staining of the expres sion of MMP-13 in articular cartilage of sections. (D) Immunohistochemical staining of the expression of Aggrecan in articular cartilage of sections. (E) Quantitative analysis of Osteoarthritis Research Society International (OARSI) histology scoring. (F) Quantitative analysis of MANKIN grading. (G) Quantita tive analysis of percentage of cells with positive staining for MMP-13 in articular cartilage. (H) Quantitative analysis of percentage of cells with positive staining for Aggrecan in articular cartilage. (I) Representative µCT images about 2D images of longitudinal section femurs and <t>3D</t> images of distal femur. The coloring parts represent the regions of interest (ROI) for analysis. Quantification of femoral BMD, BV/TV, Tb.Th, Tb.N and Tb.Sp in indicated groups, n = 7. (J) Representative µCT images about 2D images of longitudinal section tibias and 3D images <t>of</t> <t>proximal</t> tibias. The coloring parts represent the ROI for analysis. Quantification of tibial BMD, BV/TV, Tb.Th, Tb.N and Tb.Sp in indicated groups, n = 7. (K) Representative images of IF staining of TRAP in the subchondral bone of the ACLTVehicle and ACLTGNPs mice. (L) Representative images of IF staining of Netrin-1 in the subchondral bone of the ACLTVehicle and ACLTGNPs mice. (M) Representative images of IF staining of CGRP+ fibers in the subchondral bone of the ACLTVehicle and ACLTGNPs mice. (N) Quantitative analysis of TRAP staining. (O) Quantitative analysis of Netrin-1staining. (P) Quantitative analysis of CGRP+ fibers staining. (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, N.S represented not significant)
    3d Model Visualization Software, supplied by Bruker Corporation, used in various techniques. Bioz Stars score: 99/100, based on 10599 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/micro-ct+3d+visualization+software/Micro-CT/pm38589904-105-20-25
    Average 99 stars, based on 10599 article reviews
    3d model visualization software - by Bioz Stars, 2026-10
    99/100 stars

    Images

    1) Product Images from "Gold nanoparticles exhibit anti-osteoarthritic effects via modulating interaction of the "microbiota-gut-joint" axis."

    Article Title: Gold nanoparticles exhibit anti-osteoarthritic effects via modulating interaction of the "microbiota-gut-joint" axis.

    Journal: Journal of nanobiotechnology

    doi: 10.1186/s12951-024-02447-y

    Fig. 2 GNPs treatment alleviated ACLT-induced OA. (A) To evaluate the mitigation of GNPs on OA, C57BL/6J mice (6–8 weeks, male, 20–25 g) were selected to undergo anterior cruciate ligament transection surgery to induce the OA models, and randomly assigned to ACLTVehicle or ACLTGNPs group. The ACLTVehicle and ACLTGNPs groups were given continuous gavage with the equivalent amount of saline and GNPs for 8 weeks, respectively. (B) H&E, Safranine O and Toluidine Blue staining of femur articular cartilage in ACLTVehicle and ACLTGNPs groups. (C) Immunohistochemical staining of the expres sion of MMP-13 in articular cartilage of sections. (D) Immunohistochemical staining of the expression of Aggrecan in articular cartilage of sections. (E) Quantitative analysis of Osteoarthritis Research Society International (OARSI) histology scoring. (F) Quantitative analysis of MANKIN grading. (G) Quantita tive analysis of percentage of cells with positive staining for MMP-13 in articular cartilage. (H) Quantitative analysis of percentage of cells with positive staining for Aggrecan in articular cartilage. (I) Representative µCT images about 2D images of longitudinal section femurs and 3D images of distal femur. The coloring parts represent the regions of interest (ROI) for analysis. Quantification of femoral BMD, BV/TV, Tb.Th, Tb.N and Tb.Sp in indicated groups, n = 7. (J) Representative µCT images about 2D images of longitudinal section tibias and 3D images of proximal tibias. The coloring parts represent the ROI for analysis. Quantification of tibial BMD, BV/TV, Tb.Th, Tb.N and Tb.Sp in indicated groups, n = 7. (K) Representative images of IF staining of TRAP in the subchondral bone of the ACLTVehicle and ACLTGNPs mice. (L) Representative images of IF staining of Netrin-1 in the subchondral bone of the ACLTVehicle and ACLTGNPs mice. (M) Representative images of IF staining of CGRP+ fibers in the subchondral bone of the ACLTVehicle and ACLTGNPs mice. (N) Quantitative analysis of TRAP staining. (O) Quantitative analysis of Netrin-1staining. (P) Quantitative analysis of CGRP+ fibers staining. (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, N.S represented not significant)
    Figure Legend Snippet: Fig. 2 GNPs treatment alleviated ACLT-induced OA. (A) To evaluate the mitigation of GNPs on OA, C57BL/6J mice (6–8 weeks, male, 20–25 g) were selected to undergo anterior cruciate ligament transection surgery to induce the OA models, and randomly assigned to ACLTVehicle or ACLTGNPs group. The ACLTVehicle and ACLTGNPs groups were given continuous gavage with the equivalent amount of saline and GNPs for 8 weeks, respectively. (B) H&E, Safranine O and Toluidine Blue staining of femur articular cartilage in ACLTVehicle and ACLTGNPs groups. (C) Immunohistochemical staining of the expres sion of MMP-13 in articular cartilage of sections. (D) Immunohistochemical staining of the expression of Aggrecan in articular cartilage of sections. (E) Quantitative analysis of Osteoarthritis Research Society International (OARSI) histology scoring. (F) Quantitative analysis of MANKIN grading. (G) Quantita tive analysis of percentage of cells with positive staining for MMP-13 in articular cartilage. (H) Quantitative analysis of percentage of cells with positive staining for Aggrecan in articular cartilage. (I) Representative µCT images about 2D images of longitudinal section femurs and 3D images of distal femur. The coloring parts represent the regions of interest (ROI) for analysis. Quantification of femoral BMD, BV/TV, Tb.Th, Tb.N and Tb.Sp in indicated groups, n = 7. (J) Representative µCT images about 2D images of longitudinal section tibias and 3D images of proximal tibias. The coloring parts represent the ROI for analysis. Quantification of tibial BMD, BV/TV, Tb.Th, Tb.N and Tb.Sp in indicated groups, n = 7. (K) Representative images of IF staining of TRAP in the subchondral bone of the ACLTVehicle and ACLTGNPs mice. (L) Representative images of IF staining of Netrin-1 in the subchondral bone of the ACLTVehicle and ACLTGNPs mice. (M) Representative images of IF staining of CGRP+ fibers in the subchondral bone of the ACLTVehicle and ACLTGNPs mice. (N) Quantitative analysis of TRAP staining. (O) Quantitative analysis of Netrin-1staining. (P) Quantitative analysis of CGRP+ fibers staining. (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, N.S represented not significant)

    Techniques Used: Saline, Staining, Immunohistochemical staining, Expressing

    Fig. 3 The protective effect of GNPs against ACLT-induced OA disappeared after gut microbiota consumption. (A) The schematic diagram of gut micro biota consumption experiments. (B) H&E, Safranine O and Toluidine Blue staining of femur articular cartilage in ACLTVehicle+ABX and ACLTGNPs+ABX groups. (C) Immunohistochemical staining of the expression of MMP-13 in articular cartilage of sections. (D) Immunohistochemical staining of the expression of Ag grecan in articular cartilage of sections. (E) Quantitative analysis of OARSI histology scoring. (F) Quantitative analysis of MANKIN grading. (G) Quantitative analysis of percentage of cells with positive staining for MMP-13 in articular cartilage. (H) Quantitative analysis of percentage of cells with positive staining for Aggrecan in articular cartilage. (I) Representative µCT images about 2D images of longitudinal section femurs and 3D images of distal femurs. The col oring parts represent the ROI for analysis. Quantification of femoral BMD, BV/TV, Tb.Th, Tb.N and Tb.Sp in indicated groups, n = 10. (J) Representative µCT images about 2D images of longitudinal section tibias and 3D images of proximal tibias. The coloring parts represent the ROI for analysis. Quantification of tibial BMD, BV/TV, Tb.Th, Tb.N and Tb.Sp in indicated groups, n = 10. (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, N.S represented not significant)
    Figure Legend Snippet: Fig. 3 The protective effect of GNPs against ACLT-induced OA disappeared after gut microbiota consumption. (A) The schematic diagram of gut micro biota consumption experiments. (B) H&E, Safranine O and Toluidine Blue staining of femur articular cartilage in ACLTVehicle+ABX and ACLTGNPs+ABX groups. (C) Immunohistochemical staining of the expression of MMP-13 in articular cartilage of sections. (D) Immunohistochemical staining of the expression of Ag grecan in articular cartilage of sections. (E) Quantitative analysis of OARSI histology scoring. (F) Quantitative analysis of MANKIN grading. (G) Quantitative analysis of percentage of cells with positive staining for MMP-13 in articular cartilage. (H) Quantitative analysis of percentage of cells with positive staining for Aggrecan in articular cartilage. (I) Representative µCT images about 2D images of longitudinal section femurs and 3D images of distal femurs. The col oring parts represent the ROI for analysis. Quantification of femoral BMD, BV/TV, Tb.Th, Tb.N and Tb.Sp in indicated groups, n = 10. (J) Representative µCT images about 2D images of longitudinal section tibias and 3D images of proximal tibias. The coloring parts represent the ROI for analysis. Quantification of tibial BMD, BV/TV, Tb.Th, Tb.N and Tb.Sp in indicated groups, n = 10. (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, N.S represented not significant)

    Techniques Used: Staining, Immunohistochemical staining, Expressing

    Fig. 4 Fecal microbiota transplantation alleviated ACLT-induced OA. (A) The schematic diagram of the FMT experiments. (B) H&E, Safranine O and Tolu idine Blue staining of femur articular cartilage in ACLTVehicle+FMT and ACLTGNPs+FMT group. (C) Immunohistochemical staining of the expression of MMP- 13 in articular cartilage of sections. (D) Immunohistochemical staining of the expression of Aggrecan in articular cartilage of sections. (E) Quantitative analysis of OARSI histology scoring. (F) Quantitative analysis of MANKIN grading. (G) Quantitative analysis of percentage of cells with positive staining for MMP-13 in articular cartilage. (H) Quantitative analysis of percentage of cells with positive staining for Aggrecan in articular cartilage. (I) Representative µCT images about 2D images of longitudinal section femurs and 3D images of distal femurs. The coloring parts represent the ROI for analysis. Quantifica tion of femoral BMD, BV/TV, Tb.Th, Tb.N and Tb.Sp in indicated groups, n = 5. (J) Representative µCT images about 2D images of longitudinal section tibias and 3D images of proximal tibias. The coloring parts represent the ROI for analysis. Quantification of tibial BMD, BV/TV, Tb.Th, Tb.N and Tb.Sp in indicated groups, n = 5. (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, N.S represented not significant)
    Figure Legend Snippet: Fig. 4 Fecal microbiota transplantation alleviated ACLT-induced OA. (A) The schematic diagram of the FMT experiments. (B) H&E, Safranine O and Tolu idine Blue staining of femur articular cartilage in ACLTVehicle+FMT and ACLTGNPs+FMT group. (C) Immunohistochemical staining of the expression of MMP- 13 in articular cartilage of sections. (D) Immunohistochemical staining of the expression of Aggrecan in articular cartilage of sections. (E) Quantitative analysis of OARSI histology scoring. (F) Quantitative analysis of MANKIN grading. (G) Quantitative analysis of percentage of cells with positive staining for MMP-13 in articular cartilage. (H) Quantitative analysis of percentage of cells with positive staining for Aggrecan in articular cartilage. (I) Representative µCT images about 2D images of longitudinal section femurs and 3D images of distal femurs. The coloring parts represent the ROI for analysis. Quantifica tion of femoral BMD, BV/TV, Tb.Th, Tb.N and Tb.Sp in indicated groups, n = 5. (J) Representative µCT images about 2D images of longitudinal section tibias and 3D images of proximal tibias. The coloring parts represent the ROI for analysis. Quantification of tibial BMD, BV/TV, Tb.Th, Tb.N and Tb.Sp in indicated groups, n = 5. (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, N.S represented not significant)

    Techniques Used: Transplantation Assay, Staining, Immunohistochemical staining, Expressing

    Related Articles

    Control:

    Article Title: Soluble Nanomedicine Against Soluble Neurosensitizers to Effectively Relieve Osteoarthritis Pain and Joint Damage
    Article Snippet: .. The knee joint specimens from both the LAC‐treated and control groups were securely mounted in ex vivo micro‐CT (IVIS, Spectrum, Bruker, Germany) to ensure proper positioning for imaging. ..

    Ex Vivo:

    Article Title: Soluble Nanomedicine Against Soluble Neurosensitizers to Effectively Relieve Osteoarthritis Pain and Joint Damage
    Article Snippet: .. The knee joint specimens from both the LAC‐treated and control groups were securely mounted in ex vivo micro‐CT (IVIS, Spectrum, Bruker, Germany) to ensure proper positioning for imaging. ..

    Micro-CT:

    Article Title: Soluble Nanomedicine Against Soluble Neurosensitizers to Effectively Relieve Osteoarthritis Pain and Joint Damage
    Article Snippet: .. The knee joint specimens from both the LAC‐treated and control groups were securely mounted in ex vivo micro‐CT (IVIS, Spectrum, Bruker, Germany) to ensure proper positioning for imaging. ..

    Article Title: Moxibustion Regulates the Imbalance of Cartilage Degradation and Repair in Knee Osteoarthritis Rabbits via the SDF-1/CXCR4 Axis: Is Stimulating Heat-Sensitized Acupoints More Efficacious?
    Article Snippet: Band intensity was analyzed using ImageJ software, with GAPDH serving as the internal reference protein. .. The right knee joints of the New Zealand rabbits were fixed in 4% paraformaldehyde (Servicebio, Guangzhou, China) for 24 hours and subsequently scanned using a Micro-CT system (Bruker, Germany). ..

    Article Title: Anti-migratory chitosan/BaSO4 markers with hydration in situ for long-term multimodal breast tissue imaging
    Article Snippet: Finally, compression tests (crosshead speed 1 mm/min) were conducted on the hydrated samples using a universal testing machine (Instron 34SC-1, USA) to assess their compressive strength and toughness under simulated physiological conditions (PBS, pH 7.4, 37 ◦C). .. The xerogel samples, both in their initial dry state (5 mm in length and 1.5 mm in diameter) and corresponding hydrated state, were imaged via a high-resolution micro-CT scanner (Bruker SkyScan 1272, USA). ..

    Article Title: Divergent pathways of straw versus biochar integration with manure to enhance SOC sequestration in coastal saline soils
    Article Snippet: .. Three representative large macroaggregates from each sample were scanned using a micro-CT scanner (Bruker Skyscan 1272, Germany) at 100 keV and 100 μA. ..

    Article Title: Establishment of a novel asymmetric force degenerative lumbar scoliosis aging bipedal rat model
    Article Snippet: .. After scanning, the micro-CT images of the spinal specimens were imported into the NRecon reconstruction software (version 1.7; Bruker Corporation) to obtain cross-sectional images of the L1-L6 vertebrae of the rat spinal specimens. ..

    Imaging:

    Article Title: Soluble Nanomedicine Against Soluble Neurosensitizers to Effectively Relieve Osteoarthritis Pain and Joint Damage
    Article Snippet: .. The knee joint specimens from both the LAC‐treated and control groups were securely mounted in ex vivo micro‐CT (IVIS, Spectrum, Bruker, Germany) to ensure proper positioning for imaging. ..

    Software:

    Article Title: OXR1 is a dual redox-mitochondrial checkpoint governing osteoclast differentiation and bone loss in an osteoporosis mouse model.
    Article Snippet: .. Reconstruction of the acquired images was performed with CTvox software (Bruker microCT, Kontich, Belgium), utilizing a ring artifact correction of 7, a smoothing factor of 2, and a beam hardening correction of 40%. ..

    Article Title: Establishment of a novel asymmetric force degenerative lumbar scoliosis aging bipedal rat model
    Article Snippet: .. After scanning, the micro-CT images of the spinal specimens were imported into the NRecon reconstruction software (version 1.7; Bruker Corporation) to obtain cross-sectional images of the L1-L6 vertebrae of the rat spinal specimens. ..

    other:

    Article Title: IL-4 and arginine synergy accelerates early bone regeneration via enhanced efferocytosis and ameliorated microenvironment
    Article Snippet: The fixed femoral specimens were examined and imaged using Micro-CT (Skyscan 1276, Bruker, Kontich, Belgium) to evaluate the newly formed bone within the defect area.



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    Fig. 2 GNPs treatment alleviated ACLT-induced OA. (A) To evaluate the mitigation of GNPs on OA, C57BL/6J mice (6–8 weeks, male, 20–25 g) were selected to undergo anterior cruciate ligament transection surgery to induce the OA models, and randomly assigned to ACLTVehicle or ACLTGNPs group. The ACLTVehicle and ACLTGNPs groups were given continuous gavage with the equivalent amount of saline and GNPs for 8 weeks, respectively. (B) H&E, Safranine O and Toluidine Blue staining of femur articular cartilage in ACLTVehicle and ACLTGNPs groups. (C) Immunohistochemical staining of the expres sion of MMP-13 in articular cartilage of sections. (D) Immunohistochemical staining of the expression of Aggrecan in articular cartilage of sections. (E) Quantitative analysis of Osteoarthritis Research Society International (OARSI) histology scoring. (F) Quantitative analysis of MANKIN grading. (G) Quantita tive analysis of percentage of cells with positive staining for MMP-13 in articular cartilage. (H) Quantitative analysis of percentage of cells with positive staining for Aggrecan in articular cartilage. (I) Representative µCT images about 2D images of longitudinal section femurs and <t>3D</t> images of distal femur. The coloring parts represent the regions of interest (ROI) for analysis. Quantification of femoral BMD, BV/TV, Tb.Th, Tb.N and Tb.Sp in indicated groups, n = 7. (J) Representative µCT images about 2D images of longitudinal section tibias and 3D images <t>of</t> <t>proximal</t> tibias. The coloring parts represent the ROI for analysis. Quantification of tibial BMD, BV/TV, Tb.Th, Tb.N and Tb.Sp in indicated groups, n = 7. (K) Representative images of IF staining of TRAP in the subchondral bone of the ACLTVehicle and ACLTGNPs mice. (L) Representative images of IF staining of Netrin-1 in the subchondral bone of the ACLTVehicle and ACLTGNPs mice. (M) Representative images of IF staining of CGRP+ fibers in the subchondral bone of the ACLTVehicle and ACLTGNPs mice. (N) Quantitative analysis of TRAP staining. (O) Quantitative analysis of Netrin-1staining. (P) Quantitative analysis of CGRP+ fibers staining. (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, N.S represented not significant)
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    Image Search Results


    Fig. 2 GNPs treatment alleviated ACLT-induced OA. (A) To evaluate the mitigation of GNPs on OA, C57BL/6J mice (6–8 weeks, male, 20–25 g) were selected to undergo anterior cruciate ligament transection surgery to induce the OA models, and randomly assigned to ACLTVehicle or ACLTGNPs group. The ACLTVehicle and ACLTGNPs groups were given continuous gavage with the equivalent amount of saline and GNPs for 8 weeks, respectively. (B) H&E, Safranine O and Toluidine Blue staining of femur articular cartilage in ACLTVehicle and ACLTGNPs groups. (C) Immunohistochemical staining of the expres sion of MMP-13 in articular cartilage of sections. (D) Immunohistochemical staining of the expression of Aggrecan in articular cartilage of sections. (E) Quantitative analysis of Osteoarthritis Research Society International (OARSI) histology scoring. (F) Quantitative analysis of MANKIN grading. (G) Quantita tive analysis of percentage of cells with positive staining for MMP-13 in articular cartilage. (H) Quantitative analysis of percentage of cells with positive staining for Aggrecan in articular cartilage. (I) Representative µCT images about 2D images of longitudinal section femurs and 3D images of distal femur. The coloring parts represent the regions of interest (ROI) for analysis. Quantification of femoral BMD, BV/TV, Tb.Th, Tb.N and Tb.Sp in indicated groups, n = 7. (J) Representative µCT images about 2D images of longitudinal section tibias and 3D images of proximal tibias. The coloring parts represent the ROI for analysis. Quantification of tibial BMD, BV/TV, Tb.Th, Tb.N and Tb.Sp in indicated groups, n = 7. (K) Representative images of IF staining of TRAP in the subchondral bone of the ACLTVehicle and ACLTGNPs mice. (L) Representative images of IF staining of Netrin-1 in the subchondral bone of the ACLTVehicle and ACLTGNPs mice. (M) Representative images of IF staining of CGRP+ fibers in the subchondral bone of the ACLTVehicle and ACLTGNPs mice. (N) Quantitative analysis of TRAP staining. (O) Quantitative analysis of Netrin-1staining. (P) Quantitative analysis of CGRP+ fibers staining. (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, N.S represented not significant)

    Journal: Journal of nanobiotechnology

    Article Title: Gold nanoparticles exhibit anti-osteoarthritic effects via modulating interaction of the "microbiota-gut-joint" axis.

    doi: 10.1186/s12951-024-02447-y

    Figure Lengend Snippet: Fig. 2 GNPs treatment alleviated ACLT-induced OA. (A) To evaluate the mitigation of GNPs on OA, C57BL/6J mice (6–8 weeks, male, 20–25 g) were selected to undergo anterior cruciate ligament transection surgery to induce the OA models, and randomly assigned to ACLTVehicle or ACLTGNPs group. The ACLTVehicle and ACLTGNPs groups were given continuous gavage with the equivalent amount of saline and GNPs for 8 weeks, respectively. (B) H&E, Safranine O and Toluidine Blue staining of femur articular cartilage in ACLTVehicle and ACLTGNPs groups. (C) Immunohistochemical staining of the expres sion of MMP-13 in articular cartilage of sections. (D) Immunohistochemical staining of the expression of Aggrecan in articular cartilage of sections. (E) Quantitative analysis of Osteoarthritis Research Society International (OARSI) histology scoring. (F) Quantitative analysis of MANKIN grading. (G) Quantita tive analysis of percentage of cells with positive staining for MMP-13 in articular cartilage. (H) Quantitative analysis of percentage of cells with positive staining for Aggrecan in articular cartilage. (I) Representative µCT images about 2D images of longitudinal section femurs and 3D images of distal femur. The coloring parts represent the regions of interest (ROI) for analysis. Quantification of femoral BMD, BV/TV, Tb.Th, Tb.N and Tb.Sp in indicated groups, n = 7. (J) Representative µCT images about 2D images of longitudinal section tibias and 3D images of proximal tibias. The coloring parts represent the ROI for analysis. Quantification of tibial BMD, BV/TV, Tb.Th, Tb.N and Tb.Sp in indicated groups, n = 7. (K) Representative images of IF staining of TRAP in the subchondral bone of the ACLTVehicle and ACLTGNPs mice. (L) Representative images of IF staining of Netrin-1 in the subchondral bone of the ACLTVehicle and ACLTGNPs mice. (M) Representative images of IF staining of CGRP+ fibers in the subchondral bone of the ACLTVehicle and ACLTGNPs mice. (N) Quantitative analysis of TRAP staining. (O) Quantitative analysis of Netrin-1staining. (P) Quantitative analysis of CGRP+ fibers staining. (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, N.S represented not significant)

    Article Snippet: The subchondral bone regions of the distal femur and proximal tibia were selected as the region of interest (ROI), and 3D model visualization software (CTvox, Bruker micro-CT, ver.3.3.1.0) was used for 3-dimensional histomorphometric analysis.

    Techniques: Saline, Staining, Immunohistochemical staining, Expressing

    Fig. 3 The protective effect of GNPs against ACLT-induced OA disappeared after gut microbiota consumption. (A) The schematic diagram of gut micro biota consumption experiments. (B) H&E, Safranine O and Toluidine Blue staining of femur articular cartilage in ACLTVehicle+ABX and ACLTGNPs+ABX groups. (C) Immunohistochemical staining of the expression of MMP-13 in articular cartilage of sections. (D) Immunohistochemical staining of the expression of Ag grecan in articular cartilage of sections. (E) Quantitative analysis of OARSI histology scoring. (F) Quantitative analysis of MANKIN grading. (G) Quantitative analysis of percentage of cells with positive staining for MMP-13 in articular cartilage. (H) Quantitative analysis of percentage of cells with positive staining for Aggrecan in articular cartilage. (I) Representative µCT images about 2D images of longitudinal section femurs and 3D images of distal femurs. The col oring parts represent the ROI for analysis. Quantification of femoral BMD, BV/TV, Tb.Th, Tb.N and Tb.Sp in indicated groups, n = 10. (J) Representative µCT images about 2D images of longitudinal section tibias and 3D images of proximal tibias. The coloring parts represent the ROI for analysis. Quantification of tibial BMD, BV/TV, Tb.Th, Tb.N and Tb.Sp in indicated groups, n = 10. (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, N.S represented not significant)

    Journal: Journal of nanobiotechnology

    Article Title: Gold nanoparticles exhibit anti-osteoarthritic effects via modulating interaction of the "microbiota-gut-joint" axis.

    doi: 10.1186/s12951-024-02447-y

    Figure Lengend Snippet: Fig. 3 The protective effect of GNPs against ACLT-induced OA disappeared after gut microbiota consumption. (A) The schematic diagram of gut micro biota consumption experiments. (B) H&E, Safranine O and Toluidine Blue staining of femur articular cartilage in ACLTVehicle+ABX and ACLTGNPs+ABX groups. (C) Immunohistochemical staining of the expression of MMP-13 in articular cartilage of sections. (D) Immunohistochemical staining of the expression of Ag grecan in articular cartilage of sections. (E) Quantitative analysis of OARSI histology scoring. (F) Quantitative analysis of MANKIN grading. (G) Quantitative analysis of percentage of cells with positive staining for MMP-13 in articular cartilage. (H) Quantitative analysis of percentage of cells with positive staining for Aggrecan in articular cartilage. (I) Representative µCT images about 2D images of longitudinal section femurs and 3D images of distal femurs. The col oring parts represent the ROI for analysis. Quantification of femoral BMD, BV/TV, Tb.Th, Tb.N and Tb.Sp in indicated groups, n = 10. (J) Representative µCT images about 2D images of longitudinal section tibias and 3D images of proximal tibias. The coloring parts represent the ROI for analysis. Quantification of tibial BMD, BV/TV, Tb.Th, Tb.N and Tb.Sp in indicated groups, n = 10. (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, N.S represented not significant)

    Article Snippet: The subchondral bone regions of the distal femur and proximal tibia were selected as the region of interest (ROI), and 3D model visualization software (CTvox, Bruker micro-CT, ver.3.3.1.0) was used for 3-dimensional histomorphometric analysis.

    Techniques: Staining, Immunohistochemical staining, Expressing

    Fig. 4 Fecal microbiota transplantation alleviated ACLT-induced OA. (A) The schematic diagram of the FMT experiments. (B) H&E, Safranine O and Tolu idine Blue staining of femur articular cartilage in ACLTVehicle+FMT and ACLTGNPs+FMT group. (C) Immunohistochemical staining of the expression of MMP- 13 in articular cartilage of sections. (D) Immunohistochemical staining of the expression of Aggrecan in articular cartilage of sections. (E) Quantitative analysis of OARSI histology scoring. (F) Quantitative analysis of MANKIN grading. (G) Quantitative analysis of percentage of cells with positive staining for MMP-13 in articular cartilage. (H) Quantitative analysis of percentage of cells with positive staining for Aggrecan in articular cartilage. (I) Representative µCT images about 2D images of longitudinal section femurs and 3D images of distal femurs. The coloring parts represent the ROI for analysis. Quantifica tion of femoral BMD, BV/TV, Tb.Th, Tb.N and Tb.Sp in indicated groups, n = 5. (J) Representative µCT images about 2D images of longitudinal section tibias and 3D images of proximal tibias. The coloring parts represent the ROI for analysis. Quantification of tibial BMD, BV/TV, Tb.Th, Tb.N and Tb.Sp in indicated groups, n = 5. (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, N.S represented not significant)

    Journal: Journal of nanobiotechnology

    Article Title: Gold nanoparticles exhibit anti-osteoarthritic effects via modulating interaction of the "microbiota-gut-joint" axis.

    doi: 10.1186/s12951-024-02447-y

    Figure Lengend Snippet: Fig. 4 Fecal microbiota transplantation alleviated ACLT-induced OA. (A) The schematic diagram of the FMT experiments. (B) H&E, Safranine O and Tolu idine Blue staining of femur articular cartilage in ACLTVehicle+FMT and ACLTGNPs+FMT group. (C) Immunohistochemical staining of the expression of MMP- 13 in articular cartilage of sections. (D) Immunohistochemical staining of the expression of Aggrecan in articular cartilage of sections. (E) Quantitative analysis of OARSI histology scoring. (F) Quantitative analysis of MANKIN grading. (G) Quantitative analysis of percentage of cells with positive staining for MMP-13 in articular cartilage. (H) Quantitative analysis of percentage of cells with positive staining for Aggrecan in articular cartilage. (I) Representative µCT images about 2D images of longitudinal section femurs and 3D images of distal femurs. The coloring parts represent the ROI for analysis. Quantifica tion of femoral BMD, BV/TV, Tb.Th, Tb.N and Tb.Sp in indicated groups, n = 5. (J) Representative µCT images about 2D images of longitudinal section tibias and 3D images of proximal tibias. The coloring parts represent the ROI for analysis. Quantification of tibial BMD, BV/TV, Tb.Th, Tb.N and Tb.Sp in indicated groups, n = 5. (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, N.S represented not significant)

    Article Snippet: The subchondral bone regions of the distal femur and proximal tibia were selected as the region of interest (ROI), and 3D model visualization software (CTvox, Bruker micro-CT, ver.3.3.1.0) was used for 3-dimensional histomorphometric analysis.

    Techniques: Transplantation Assay, Staining, Immunohistochemical staining, Expressing