3d model visualization software (Bruker Corporation)
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3d Model Visualization Software, supplied by Bruker Corporation, used in various techniques. Bioz Stars score: 99/100, based on 10599 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/micro-ct+3d+visualization+software/Micro-CT/pm38589904-105-20-25
Average 99 stars, based on 10599 article reviews
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1) Product Images from "Gold nanoparticles exhibit anti-osteoarthritic effects via modulating interaction of the "microbiota-gut-joint" axis."
Article Title: Gold nanoparticles exhibit anti-osteoarthritic effects via modulating interaction of the "microbiota-gut-joint" axis.
Journal: Journal of nanobiotechnology
doi: 10.1186/s12951-024-02447-y
Figure Legend Snippet: Fig. 2 GNPs treatment alleviated ACLT-induced OA. (A) To evaluate the mitigation of GNPs on OA, C57BL/6J mice (6–8 weeks, male, 20–25 g) were selected to undergo anterior cruciate ligament transection surgery to induce the OA models, and randomly assigned to ACLTVehicle or ACLTGNPs group. The ACLTVehicle and ACLTGNPs groups were given continuous gavage with the equivalent amount of saline and GNPs for 8 weeks, respectively. (B) H&E, Safranine O and Toluidine Blue staining of femur articular cartilage in ACLTVehicle and ACLTGNPs groups. (C) Immunohistochemical staining of the expres sion of MMP-13 in articular cartilage of sections. (D) Immunohistochemical staining of the expression of Aggrecan in articular cartilage of sections. (E) Quantitative analysis of Osteoarthritis Research Society International (OARSI) histology scoring. (F) Quantitative analysis of MANKIN grading. (G) Quantita tive analysis of percentage of cells with positive staining for MMP-13 in articular cartilage. (H) Quantitative analysis of percentage of cells with positive staining for Aggrecan in articular cartilage. (I) Representative µCT images about 2D images of longitudinal section femurs and 3D images of distal femur. The coloring parts represent the regions of interest (ROI) for analysis. Quantification of femoral BMD, BV/TV, Tb.Th, Tb.N and Tb.Sp in indicated groups, n = 7. (J) Representative µCT images about 2D images of longitudinal section tibias and 3D images of proximal tibias. The coloring parts represent the ROI for analysis. Quantification of tibial BMD, BV/TV, Tb.Th, Tb.N and Tb.Sp in indicated groups, n = 7. (K) Representative images of IF staining of TRAP in the subchondral bone of the ACLTVehicle and ACLTGNPs mice. (L) Representative images of IF staining of Netrin-1 in the subchondral bone of the ACLTVehicle and ACLTGNPs mice. (M) Representative images of IF staining of CGRP+ fibers in the subchondral bone of the ACLTVehicle and ACLTGNPs mice. (N) Quantitative analysis of TRAP staining. (O) Quantitative analysis of Netrin-1staining. (P) Quantitative analysis of CGRP+ fibers staining. (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, N.S represented not significant)
Techniques Used: Saline, Staining, Immunohistochemical staining, Expressing
Figure Legend Snippet: Fig. 3 The protective effect of GNPs against ACLT-induced OA disappeared after gut microbiota consumption. (A) The schematic diagram of gut micro biota consumption experiments. (B) H&E, Safranine O and Toluidine Blue staining of femur articular cartilage in ACLTVehicle+ABX and ACLTGNPs+ABX groups. (C) Immunohistochemical staining of the expression of MMP-13 in articular cartilage of sections. (D) Immunohistochemical staining of the expression of Ag grecan in articular cartilage of sections. (E) Quantitative analysis of OARSI histology scoring. (F) Quantitative analysis of MANKIN grading. (G) Quantitative analysis of percentage of cells with positive staining for MMP-13 in articular cartilage. (H) Quantitative analysis of percentage of cells with positive staining for Aggrecan in articular cartilage. (I) Representative µCT images about 2D images of longitudinal section femurs and 3D images of distal femurs. The col oring parts represent the ROI for analysis. Quantification of femoral BMD, BV/TV, Tb.Th, Tb.N and Tb.Sp in indicated groups, n = 10. (J) Representative µCT images about 2D images of longitudinal section tibias and 3D images of proximal tibias. The coloring parts represent the ROI for analysis. Quantification of tibial BMD, BV/TV, Tb.Th, Tb.N and Tb.Sp in indicated groups, n = 10. (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, N.S represented not significant)
Techniques Used: Staining, Immunohistochemical staining, Expressing
Figure Legend Snippet: Fig. 4 Fecal microbiota transplantation alleviated ACLT-induced OA. (A) The schematic diagram of the FMT experiments. (B) H&E, Safranine O and Tolu idine Blue staining of femur articular cartilage in ACLTVehicle+FMT and ACLTGNPs+FMT group. (C) Immunohistochemical staining of the expression of MMP- 13 in articular cartilage of sections. (D) Immunohistochemical staining of the expression of Aggrecan in articular cartilage of sections. (E) Quantitative analysis of OARSI histology scoring. (F) Quantitative analysis of MANKIN grading. (G) Quantitative analysis of percentage of cells with positive staining for MMP-13 in articular cartilage. (H) Quantitative analysis of percentage of cells with positive staining for Aggrecan in articular cartilage. (I) Representative µCT images about 2D images of longitudinal section femurs and 3D images of distal femurs. The coloring parts represent the ROI for analysis. Quantifica tion of femoral BMD, BV/TV, Tb.Th, Tb.N and Tb.Sp in indicated groups, n = 5. (J) Representative µCT images about 2D images of longitudinal section tibias and 3D images of proximal tibias. The coloring parts represent the ROI for analysis. Quantification of tibial BMD, BV/TV, Tb.Th, Tb.N and Tb.Sp in indicated groups, n = 5. (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, N.S represented not significant)
Techniques Used: Transplantation Assay, Staining, Immunohistochemical staining, Expressing
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