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methyledge® bisulfite conversion system kit (Promega)

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Promega methyledge® bisulfite conversion system kit
Methyledge® Bisulfite Conversion System Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/methyledge+conversion+system+kit/methyledge+bisulfite+conversion+system/pm37537185-229-14-20
Average 90 stars, based on 1 article reviews

methyledge® bisulfite conversion system kit - by Bioz Stars, 2026-11

90/100 stars

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Article Title: Gene target region enrichment method and kit
Article Snippet: Reagents and Materials The bisulfite conversion kit was purchased from Promega.

Article Title: DNA methylation biomarker panels for differentiating various liver adenocarcinomas, including hepatocellular carcinoma, cholangiocarcinoma, colorectal liver metastases and pancreatic adenocarcinoma liver metastases.
Article Snippet: Bisulfite conversion of 400 ng DNA isolates was performed using the MethylEdge® Bisulfite Conversion System (Promega) according to the manufacturer’s instructions and eluted in a volume of 20 μL elution buffer.

Article Title: Methylation-based forensic age estimation in blood, buccal cells, saliva and semen: A comparison of two technologies.
Article Snippet: Bisulfite conversion was subsequently performed on 20 μl of extracted DNA with the MethylEdge® Bisulfite Conversion System (Promega) and eluted in 10 μl elution buffer.

Article Title: Strong nuclear expression of HOXB13 is a reliable surrogate marker for DNA methylome profiling to distinguish myxopapillary ependymoma from spinal ependymoma.
Article Snippet: In total, 200 ng of DNA from each tumor was treated with sodium bisulfite using the MethylEdge® Bisulfite Conversion System (Promega, Walldorf, Germany).

Concentration Assay:

Article Title: DNA Methylation Panels for the Differentiation of Lung and Gastric Adenocarcinomas from Other Common Primary Adenocarcinomas.
Article Snippet: The concentration of the isolated DNA was measured spectrophotometrically using a NanoDrop One Spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA) and a QubitTM 4 Fluorometer. .. Bisulfite conversion of the DNA was performed using the MethylEdge® Bisulfite Conversion System (Promega Corporation, Madison, WI, USA), following the manufacturer’s instructions, resulting in a final eluted concentration of 20 ng/μL. .. For the methylation-sensitive high resolution melt (MS-HRM) experiment, primers were designed using Methyl Primer Express Software v1.0 (Thermo Fisher Scientific, Waltham, MA, USA), based on the bisulfite-converted DNA sequence, to amplify both methylated and unmethylated DNA.

Article Title: DNA Methylation Panels for the Differentiation of Lung and Gastric Adenocarcinomas from Other Common Primary Adenocarcinomas
Article Snippet: The concentration of the isolated DNA was measured spectrophotometrically using a NanoDrop One Spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA) and a QubitTM 4 Fluorometer. .. Bisulfite conversion of the DNA was performed using the MethylEdge ® Bisulfite Conversion System (Promega Corporation, Madison, WI, USA), following the manufacturer’s instructions, resulting in a final eluted concentration of 20 ng/μL. .. For the methylation-sensitive high resolution melt (MS-HRM) experiment, primers were designed using Methyl Primer Express Software v1.0 (Thermo Fisher Scientific, Waltham, MA, USA), based on the bisulfite-converted DNA sequence, to amplify both methylated and unmethylated DNA.

Analysis:

Article Title: Characterizing Genetic, Epigenetic, Nutritional, and Clinico-Biochemical Profile of Women With Polycystic Ovary Syndrome: A Case–Control Study
Article Snippet: .. For the qMSP analysis, the DNA samples were subjected to bisulfite conversion using the MethylEdge Bisulfite Conversion (Promega, Cat. No. N1301). ..

Bisulfite Methylation Profiling:

Clinical Proteomics:

Article Title: Gene target region enrichment method and kit
Article Snippet: .. Convert the plasma samples of healthy individuals using the Promega bisulfite conversion system. ..