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methylation analysis using  (Pyrosequencing Inc)

 
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    Pyrosequencing Inc methylation analysis using
    Methylation Analysis Using, supplied by Pyrosequencing Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/methylation+analysis+using/methylation+analysis/pm40490796-5-3-6
    Average 90 stars, based on 1 article reviews
    methylation analysis using - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Methylation:

    Article Title: Unbiased DNA methylation markers define an extensive field defect in histologically normal prostate tissues associated with prostate cancer: new biomarkers for men with prostate cancer
    Article Snippet: .. TABLE 5 Subject clinical and pathological characteristics Methylation Array Pyrosequencing NTA TA NTA TA T, TAA, TAD Number 5 4 14 11 26 Age (yr) 63 61 60 59 58 (55~81) (57~64) (55~70) (51~67) (44~69) Tumor 6.3 5.1 27.1 Volume (%) Gleason grade Intermediate 4 6 16 High 10 Pathological stage T2 3 T2a 1 1 T2b 2 T2c 3 6 14 T3a 1 1 2 T3b 4 PSA (ng/ml) 7.7 59 6.9 NTA: non-tumor-associated normal. ..

    Article Title: Novel Transcriptional and DNA Methylation Abnormalities of SORT1 Gene in Non-Small Cell Lung Cancer
    Article Snippet: .. Pyrosequencing methylation analysis assays were designed to cover multiple CpGs in the promoter and 5′-UTR regions of SORT1A and SORT1B genes to allow for quantitation of their DNA methylation levels in NSCLC samples and cell lines. ..

    Article Title: Multi-locus methylation analyses reveal GNAS methylation defects in three patients with the Beckwith-Wiedemann syndrome phenotype and no molecular defects in the 11p15.5 imprinted region.
    Article Snippet: .. Results We conducted methylation analysis using pyrosequencing in 77 patients showing the BWS-phenotype without molecular defects in the 11p15.5 imprinted region. ..

    Article Title: Unveiling the role of MGMT and DAPK hypermethylation in response to anti-EGFR agents: Molecular insights for advancing HNSCC treatment.
    Article Snippet: Molecular Oncology Research Center, Barretos Cancer Hospital – Pio XII, Barretos, Brazil Barretos School of Health Sciences, Dr. Paulo Prata-FACISB, Barretos, Brazil Molecular Carcinogenesis Program, National Cancer Institute – INCA, Rio de Janeiro, Brazil Department of Genetics, Brazilian National Cancer Institute – INCA, Rio de Janeiro, Brazil Life and Health Sciences Research Institute – ICVS, Health Sciences School, University of Minho – Braga, Braga, Portugal

    Article Title: Clinical features and impact of p53 status on sporadic mismatch repair deficiency and Lynch syndrome in uterine cancer.
    Article Snippet: .. Finally, 39 samples underwent methylation analysis, and 32 (82.1%) had methylation levels >0.6 by pyrosequencing. ..

    Article Title: Universal penalized regression (Elastic-net) model with differentially methylated promoters for oral cancer prediction.
    Article Snippet: .. Poulin M, Zhou JY, Yan L, Shioda T. Pyrosequencing methylation analysis. ..

    Article Title: Unbiased DNA methylation markers define an extensive field defect in histologically normal prostate tissues associated with prostate cancer: new biomarkers for men with prostate cancer
    Article Snippet: .. TABLE 5 Subject clinical and pathological characteristics Methylation Array Pyrosequencing NTA TA NTA TA T, TAA, TAD Number 5 4 14 11 26 Age (yr) 63 (55~81) 61 (57~64) 60 (55~70) 59 (51~67) 58 (44~69) Tumor Volume (%) 6.3 5.1 27.1 Gleason grade Intermediate 4 6 16 High 10 Pathological stage T2 3 T2a 1 1 T2b 2 T2c 3 6 14 T3a 1 1 2 T3b 4 PSA (ng/ml) 7.7 5.9 6.9 NTA: non-tumor-associated normal, TA: tumor-associate, T: tumor, TAA: tumor-associated adjacent, TAD: tumor-associated distant. ..

    Article Title: “Crosstalk between non-coding RNAs and transcription factor LRF in non-small cell lung cancer”
    Article Snippet: .. Pyrosequencing methylation assay was utilized to assess DNA methylation status across CpG islands located at 5′ prime ends of lncRNAs' genetic loci. .. Genomic DNA was extracted from human A549, H460 and BEAS-2B cell lines with phenol:chloroform:isoamyl alcohol in a 25:24:1 ratio following standard protocol (Sigma-Aldrich Pty Ltd, An affiliate of Merck KGaA, Darmstadt, Germany) [ ].

    Quantitation Assay:

    Article Title: Novel Transcriptional and DNA Methylation Abnormalities of SORT1 Gene in Non-Small Cell Lung Cancer
    Article Snippet: .. Pyrosequencing methylation analysis assays were designed to cover multiple CpGs in the promoter and 5′-UTR regions of SORT1A and SORT1B genes to allow for quantitation of their DNA methylation levels in NSCLC samples and cell lines. ..

    DNA Methylation Assay:

    Article Title: Novel Transcriptional and DNA Methylation Abnormalities of SORT1 Gene in Non-Small Cell Lung Cancer
    Article Snippet: .. Pyrosequencing methylation analysis assays were designed to cover multiple CpGs in the promoter and 5′-UTR regions of SORT1A and SORT1B genes to allow for quantitation of their DNA methylation levels in NSCLC samples and cell lines. ..

    Article Title: “Crosstalk between non-coding RNAs and transcription factor LRF in non-small cell lung cancer”
    Article Snippet: .. Pyrosequencing methylation assay was utilized to assess DNA methylation status across CpG islands located at 5′ prime ends of lncRNAs' genetic loci. .. Genomic DNA was extracted from human A549, H460 and BEAS-2B cell lines with phenol:chloroform:isoamyl alcohol in a 25:24:1 ratio following standard protocol (Sigma-Aldrich Pty Ltd, An affiliate of Merck KGaA, Darmstadt, Germany) [ ].

    Droplet Countercurrent Chromatography:

    Article Title: Unveiling the role of MGMT and DAPK hypermethylation in response to anti-EGFR agents: Molecular insights for advancing HNSCC treatment.
    Article Snippet: Molecular Oncology Research Center, Barretos Cancer Hospital – Pio XII, Barretos, Brazil Barretos School of Health Sciences, Dr. Paulo Prata-FACISB, Barretos, Brazil Molecular Carcinogenesis Program, National Cancer Institute – INCA, Rio de Janeiro, Brazil Department of Genetics, Brazilian National Cancer Institute – INCA, Rio de Janeiro, Brazil Life and Health Sciences Research Institute – ICVS, Health Sciences School, University of Minho – Braga, Braga, Portugal

    Hot Start PCR:

    Article Title: Unveiling the role of MGMT and DAPK hypermethylation in response to anti-EGFR agents: Molecular insights for advancing HNSCC treatment.
    Article Snippet: Molecular Oncology Research Center, Barretos Cancer Hospital – Pio XII, Barretos, Brazil Barretos School of Health Sciences, Dr. Paulo Prata-FACISB, Barretos, Brazil Molecular Carcinogenesis Program, National Cancer Institute – INCA, Rio de Janeiro, Brazil Department of Genetics, Brazilian National Cancer Institute – INCA, Rio de Janeiro, Brazil Life and Health Sciences Research Institute – ICVS, Health Sciences School, University of Minho – Braga, Braga, Portugal



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    Image Search Results


    Heatmap of array-based methylation analysis using analysis method 1 . The heatmap indicates 56 aberrant DMRs out of 78 examined DMRs. Each row represents a DMR; each column represents a patient. Germline DMRs are shown on a gray background, secondary DMRs on a light green background, and unclassifiable DMRs on a white background. Methylation disturbances of DMRs are classified into seven categories based on the degree. B, Beckwith-Wiedemann syndrome; P, pseudohypoparathyroidism type 1B; O, overgrowth; S, Silver-Russell syndrome; TS, Temple syndrome; TN, transient neonatal diabetes mellitus; W, Prader-Willi syndrome; SG, small for gestational age; N, neurodevelopmental delay and/or intellectual developmental disorder; A, assisted reproductive technology; T, monozygotic monochorionic diamniotic twins; V, variants of uncertain significance; L, likely pathogenic; DMR, differential methylated region; Chr, chromosome; MML, median methylation level of CpG sites in the DMR; SD, standard deviation; mean, mean MML of 16 healthy controls

    Journal: Clinical Epigenetics

    Article Title: Comprehensive molecular and clinical findings in 29 patients with multi-locus imprinting disturbance

    doi: 10.1186/s13148-024-01744-5

    Figure Lengend Snippet: Heatmap of array-based methylation analysis using analysis method 1 . The heatmap indicates 56 aberrant DMRs out of 78 examined DMRs. Each row represents a DMR; each column represents a patient. Germline DMRs are shown on a gray background, secondary DMRs on a light green background, and unclassifiable DMRs on a white background. Methylation disturbances of DMRs are classified into seven categories based on the degree. B, Beckwith-Wiedemann syndrome; P, pseudohypoparathyroidism type 1B; O, overgrowth; S, Silver-Russell syndrome; TS, Temple syndrome; TN, transient neonatal diabetes mellitus; W, Prader-Willi syndrome; SG, small for gestational age; N, neurodevelopmental delay and/or intellectual developmental disorder; A, assisted reproductive technology; T, monozygotic monochorionic diamniotic twins; V, variants of uncertain significance; L, likely pathogenic; DMR, differential methylated region; Chr, chromosome; MML, median methylation level of CpG sites in the DMR; SD, standard deviation; mean, mean MML of 16 healthy controls

    Article Snippet: Consequently, we identified MLID in 29 patients with epimutation confirmed by methylation analysis for multiple ID-associated DMRs using pyrosequencing and/or methylation-specific multiple ligation-dependent probe amplification.

    Techniques: Methylation, Standard Deviation