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trypan blue exclusion method  (Thermo Fisher)


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    Thermo Fisher trypan blue exclusion method
    Trypan Blue Exclusion Method, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/methode/Trypan+Blue/pm42320117-308-12-16
    Average 99 stars, based on 1 article reviews
    trypan blue exclusion method - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Centrifugation:

    Article Title: Ultrasound-activated drug release with extracellular vesicles
    Article Snippet: .. To quantify loading efficiency, total rhodamine B or lidocaine content was determined by heating EV-PFP-RhB or EV-PFP-lidocaine samples at 65◦C for 5–30 min or several hours until no pellet remained after centrifugation.21 In a separate experiment, EV-PFP-RhB samples were incubated with 0.4% trypan blue (Gibco, Grand Island, NY) to evaluate the quenching of accessible rhodamine B fluorescence.23 In another separate study, EV-PFP-RhB were lysed using RIPA buffer (Thermo Fisher Scientific, Waltham, MA) for subsequent quantitative analysis, as previously described.41,42 Rhodamine B was analyzed either by UV-vis spectrophotometry, with absorbance measured from 200 to 850 nm, using a UV-vis spectrophotometer (Eppendorf, Hamburg, Germany), and the characteristic rhodamine B peak was monitored at approximately 560 nm or by fluorescence spectroscopy using a BioTek Synergy H1 plate reader (Agilent, Santa Clara, CA) at an excitation wavelength of 550 nm and an emission wavelength of 600 nm. .. Lidocaine was quantified by HPLC-MS using a Shimadzu LC-2030C (Shimadzu Corporation, Kyoto, Japan).

    Incubation:

    Article Title: Ultrasound-activated drug release with extracellular vesicles
    Article Snippet: .. To quantify loading efficiency, total rhodamine B or lidocaine content was determined by heating EV-PFP-RhB or EV-PFP-lidocaine samples at 65◦C for 5–30 min or several hours until no pellet remained after centrifugation.21 In a separate experiment, EV-PFP-RhB samples were incubated with 0.4% trypan blue (Gibco, Grand Island, NY) to evaluate the quenching of accessible rhodamine B fluorescence.23 In another separate study, EV-PFP-RhB were lysed using RIPA buffer (Thermo Fisher Scientific, Waltham, MA) for subsequent quantitative analysis, as previously described.41,42 Rhodamine B was analyzed either by UV-vis spectrophotometry, with absorbance measured from 200 to 850 nm, using a UV-vis spectrophotometer (Eppendorf, Hamburg, Germany), and the characteristic rhodamine B peak was monitored at approximately 560 nm or by fluorescence spectroscopy using a BioTek Synergy H1 plate reader (Agilent, Santa Clara, CA) at an excitation wavelength of 550 nm and an emission wavelength of 600 nm. .. Lidocaine was quantified by HPLC-MS using a Shimadzu LC-2030C (Shimadzu Corporation, Kyoto, Japan).

    Fluorescence:

    Article Title: Ultrasound-activated drug release with extracellular vesicles
    Article Snippet: .. To quantify loading efficiency, total rhodamine B or lidocaine content was determined by heating EV-PFP-RhB or EV-PFP-lidocaine samples at 65◦C for 5–30 min or several hours until no pellet remained after centrifugation.21 In a separate experiment, EV-PFP-RhB samples were incubated with 0.4% trypan blue (Gibco, Grand Island, NY) to evaluate the quenching of accessible rhodamine B fluorescence.23 In another separate study, EV-PFP-RhB were lysed using RIPA buffer (Thermo Fisher Scientific, Waltham, MA) for subsequent quantitative analysis, as previously described.41,42 Rhodamine B was analyzed either by UV-vis spectrophotometry, with absorbance measured from 200 to 850 nm, using a UV-vis spectrophotometer (Eppendorf, Hamburg, Germany), and the characteristic rhodamine B peak was monitored at approximately 560 nm or by fluorescence spectroscopy using a BioTek Synergy H1 plate reader (Agilent, Santa Clara, CA) at an excitation wavelength of 550 nm and an emission wavelength of 600 nm. .. Lidocaine was quantified by HPLC-MS using a Shimadzu LC-2030C (Shimadzu Corporation, Kyoto, Japan).

    Analysis:

    Article Title: Ultrasound-activated drug release with extracellular vesicles
    Article Snippet: .. To quantify loading efficiency, total rhodamine B or lidocaine content was determined by heating EV-PFP-RhB or EV-PFP-lidocaine samples at 65◦C for 5–30 min or several hours until no pellet remained after centrifugation.21 In a separate experiment, EV-PFP-RhB samples were incubated with 0.4% trypan blue (Gibco, Grand Island, NY) to evaluate the quenching of accessible rhodamine B fluorescence.23 In another separate study, EV-PFP-RhB were lysed using RIPA buffer (Thermo Fisher Scientific, Waltham, MA) for subsequent quantitative analysis, as previously described.41,42 Rhodamine B was analyzed either by UV-vis spectrophotometry, with absorbance measured from 200 to 850 nm, using a UV-vis spectrophotometer (Eppendorf, Hamburg, Germany), and the characteristic rhodamine B peak was monitored at approximately 560 nm or by fluorescence spectroscopy using a BioTek Synergy H1 plate reader (Agilent, Santa Clara, CA) at an excitation wavelength of 550 nm and an emission wavelength of 600 nm. .. Lidocaine was quantified by HPLC-MS using a Shimadzu LC-2030C (Shimadzu Corporation, Kyoto, Japan).

    Article Title: Pharmacological Myristoylation Inhibition by Zelenirstat Disrupts Growth Signalling, Energy Metabolism, and Migration in Triple-Negative Breast Cancer Cells
    Article Snippet: OCR data were the Lucid Lab online portal, processed using Microsoft Excel, and subsequently graphed and analyzed using GraphPad Prism software .. Seahorse Metabolic Analysis Cells were cultured with zelenirstat or IACS-010759 for 24 h, then collected and counted with trypan blue (Thermo Fisher). .. 40,000 live cells were plated per well in Agilent XF DMEM with the recommended supplementation.

    Spectrophotometry:

    Article Title: Ultrasound-activated drug release with extracellular vesicles
    Article Snippet: .. To quantify loading efficiency, total rhodamine B or lidocaine content was determined by heating EV-PFP-RhB or EV-PFP-lidocaine samples at 65◦C for 5–30 min or several hours until no pellet remained after centrifugation.21 In a separate experiment, EV-PFP-RhB samples were incubated with 0.4% trypan blue (Gibco, Grand Island, NY) to evaluate the quenching of accessible rhodamine B fluorescence.23 In another separate study, EV-PFP-RhB were lysed using RIPA buffer (Thermo Fisher Scientific, Waltham, MA) for subsequent quantitative analysis, as previously described.41,42 Rhodamine B was analyzed either by UV-vis spectrophotometry, with absorbance measured from 200 to 850 nm, using a UV-vis spectrophotometer (Eppendorf, Hamburg, Germany), and the characteristic rhodamine B peak was monitored at approximately 560 nm or by fluorescence spectroscopy using a BioTek Synergy H1 plate reader (Agilent, Santa Clara, CA) at an excitation wavelength of 550 nm and an emission wavelength of 600 nm. .. Lidocaine was quantified by HPLC-MS using a Shimadzu LC-2030C (Shimadzu Corporation, Kyoto, Japan).

    Spectroscopy:

    Article Title: Ultrasound-activated drug release with extracellular vesicles
    Article Snippet: .. To quantify loading efficiency, total rhodamine B or lidocaine content was determined by heating EV-PFP-RhB or EV-PFP-lidocaine samples at 65◦C for 5–30 min or several hours until no pellet remained after centrifugation.21 In a separate experiment, EV-PFP-RhB samples were incubated with 0.4% trypan blue (Gibco, Grand Island, NY) to evaluate the quenching of accessible rhodamine B fluorescence.23 In another separate study, EV-PFP-RhB were lysed using RIPA buffer (Thermo Fisher Scientific, Waltham, MA) for subsequent quantitative analysis, as previously described.41,42 Rhodamine B was analyzed either by UV-vis spectrophotometry, with absorbance measured from 200 to 850 nm, using a UV-vis spectrophotometer (Eppendorf, Hamburg, Germany), and the characteristic rhodamine B peak was monitored at approximately 560 nm or by fluorescence spectroscopy using a BioTek Synergy H1 plate reader (Agilent, Santa Clara, CA) at an excitation wavelength of 550 nm and an emission wavelength of 600 nm. .. Lidocaine was quantified by HPLC-MS using a Shimadzu LC-2030C (Shimadzu Corporation, Kyoto, Japan).

    Suspension:

    Article Title: Glyoxalase I inhibition by TLSC702 sensitizes glyoxalase I-dependent cancer cells to doxorubicin-induced cell death.
    Article Snippet: .. The resulting cell suspension was mixed with 0.4% trypan blue (Gibco, New York, USA) at a 1:1 ratio, loaded into a Countess cell counting chamber slide (Invitrogen, Thermo Fisher Scientific, Massachusetts, USA). .. The numbers of viable (trypan blue-negative) and nonviable (trypan blue-positive) cells were counted using a Countess II FL automated cell counter (Invitrogen, Thermo Fisher Scientific, Massachusetts, USA).

    Cell Counting:

    Article Title: Glyoxalase I inhibition by TLSC702 sensitizes glyoxalase I-dependent cancer cells to doxorubicin-induced cell death.
    Article Snippet: .. The resulting cell suspension was mixed with 0.4% trypan blue (Gibco, New York, USA) at a 1:1 ratio, loaded into a Countess cell counting chamber slide (Invitrogen, Thermo Fisher Scientific, Massachusetts, USA). .. The numbers of viable (trypan blue-negative) and nonviable (trypan blue-positive) cells were counted using a Countess II FL automated cell counter (Invitrogen, Thermo Fisher Scientific, Massachusetts, USA).

    Article Title: Fatty acid and cysteine metabolic interplay regulate ferroptosis and highlight xCT as a selenium-chrysin target in breast carcinoma.
    Article Snippet: Cancer metabolic remodeling impacts the entire network of metabolic pathways, and strategies that target various points within this system could contribute to successfully abrogating cancer cell survival.. Fatty acids (FAs) are essential to cancer cells because they support membrane biosynthesis during proliferation and provide energy during metabolic stress.. Fatty acid transport protein 1 (FATP1)has been shown to mediate FA uptake in breast carcinoma (BC).

    Article Title: Food safety of insect and microalgae alternative proteins: IgE cross-reactivity and cytotoxicity evaluation
    Article Snippet: Cell morphology was checked under a Leica DM IRB-E (Leica Microsystems Wetzlar GmbH, Wetzlar, Germany) inverted microscope. .. The number of viable cells was estimated using the Trypan Blue test with Countess cell counting chamber slides and automated cell counter Countess II (Invitrogen, Carlsbad, CA, USA). ..

    other:

    Article Title: Gravitational and mechanical forces shape mitochondrial translation.
    Article Snippet: After washing twice with PBS, the cells were incubated with Intercept Blocking Buffer (LI-COR Biosciences) containing 0.2% Triton X-100 for 60min at room temperature.

    Staining:

    Article Title: Fatty acid and cysteine metabolic interplay regulate ferroptosis and highlight xCT as a selenium-chrysin target in breast carcinoma.
    Article Snippet: Cancer metabolic remodeling impacts the entire network of metabolic pathways, and strategies that target various points within this system could contribute to successfully abrogating cancer cell survival.. Fatty acids (FAs) are essential to cancer cells because they support membrane biosynthesis during proliferation and provide energy during metabolic stress.. Fatty acid transport protein 1 (FATP1)has been shown to mediate FA uptake in breast carcinoma (BC).

    Article Title: Carbon Monoxide as a Cancer-Selective Anti-Neoplastic Agent in Esophageal Epithelial Cells
    Article Snippet: .. The viability and cell counts were determined using the Invitrogen Countess 3 FL Automated Cell Counter (Thermo Fisher Scientific, Waltham, MA, USA) based on the exclusion of Trypan Blue (Thermo Fisher Scientific, Vilnius, Lithuania) -stained dead cells. ..

    Cell Culture:

    Article Title: Pharmacological Myristoylation Inhibition by Zelenirstat Disrupts Growth Signalling, Energy Metabolism, and Migration in Triple-Negative Breast Cancer Cells
    Article Snippet: OCR data were the Lucid Lab online portal, processed using Microsoft Excel, and subsequently graphed and analyzed using GraphPad Prism software .. Seahorse Metabolic Analysis Cells were cultured with zelenirstat or IACS-010759 for 24 h, then collected and counted with trypan blue (Thermo Fisher). .. 40,000 live cells were plated per well in Agilent XF DMEM with the recommended supplementation.

    Automated:

    Article Title: Food safety of insect and microalgae alternative proteins: IgE cross-reactivity and cytotoxicity evaluation
    Article Snippet: Cell morphology was checked under a Leica DM IRB-E (Leica Microsystems Wetzlar GmbH, Wetzlar, Germany) inverted microscope. .. The number of viable cells was estimated using the Trypan Blue test with Countess cell counting chamber slides and automated cell counter Countess II (Invitrogen, Carlsbad, CA, USA). ..

    Article Title: Carbon Monoxide as a Cancer-Selective Anti-Neoplastic Agent in Esophageal Epithelial Cells
    Article Snippet: .. The viability and cell counts were determined using the Invitrogen Countess 3 FL Automated Cell Counter (Thermo Fisher Scientific, Waltham, MA, USA) based on the exclusion of Trypan Blue (Thermo Fisher Scientific, Vilnius, Lithuania) -stained dead cells. ..



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