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microscopy automation & image analysis software  (MetaMorph Inc)

 
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    Structured Review

    MetaMorph Inc microscopy automation & image analysis software
    Microscopy Automation & Image Analysis Software, supplied by MetaMorph Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/metamorph+software/microscopy+automation+and+image+analysis+software/pmc08203899-29-1-0
    Average 90 stars, based on 1 article reviews
    microscopy automation & image analysis software - by Bioz Stars, 2026-11
    90/100 stars

    Images

    Related Articles

    Microscopy:

    Article Title: High-Resolution C. elegans Imaging Across All Larval Stages.
    Article Snippet: Caenorhabditis elegans has become one of the most widely studied and bestunderstood animal models in biology.. Three features are key to C. elegans' success as a model organism: its invariant cell lineage, transparency, and genetic tractability.. These render it ideal for a diverse range of microscopy-based studies directly in vivo.

    Article Title: A neural tract-inspired conduit for facile, on-demand biopsy of glioblastoma
    Article Snippet: Tiled images were obtained using a Leica semi-confocal microscope fluorescent imaging system, and quantification procedures were performed for DAPI, GFP, and Ku80 antibodies using pre-defined thresholds. .. Images were processed through either Image J or MetaMorph Microscopy Image Analysis Software. ..

    Article Title: Carbene formation as a mechanism for efficient intracellular uptake of cationic antimicrobial carbon acid polymers.
    Article Snippet: .. The fluorescence images were edited by subtracting the background with MetaMorph Microscopy Automation and Image Analysis Software. ..

    Article Title: Dual regulation of IP 3 receptors by IP 3 and PIP 2 controls the transition from local to global Ca 2+ signals.
    Article Snippet: All fluorescence images and image stacks were background-corrected before analysis via subtraction of the fluorescence de- tected from a region outside the cell using MetaMorph Microscopy Automation and Image Analysis Software. .. All fluorescence images and image stacks were background-corrected before analysis via subtraction of the fluorescence de- tected from a region outside the cell using MetaMorph Microscopy Automation and Image Analysis Software. ..

    Article Title: Mutations in Parkinsonism-linked endocytic proteins synaptojanin1 and auxilin have synergistic effects on dopaminergic axonal pathology.
    Article Snippet: .. Image acquisition involved tiling of the entire section using a 20x Air lens, keeping the same laser intensity and exposure for both channels on MetaMorph Microscopy Automation and Image Analysis Software (RRID: SCR_002368). .. Stitched images were then set to the same threshold intensity for each individual channel in Fiji software (Version ImageJ 1.52p/Java 1.8.0_172 (64-bit), RRID: SCR_002285).

    Article Title: Development of Bifunctional, Raman Active Diyne-Girder Stapled α-Helical Peptides.
    Article Snippet: Stapled peptides are a unique class of cyclic αhelical peptides that are conformationally constrained via their amino acid side-chains.. They have been transformative to the field of chemical biology and peptide drug discovery through addressing many of the physicochemical limitations of linear peptides.. However, there are several issues with current chemical strategies to produce stapled peptides.

    Software:

    Article Title: High-Resolution C. elegans Imaging Across All Larval Stages.
    Article Snippet: Caenorhabditis elegans has become one of the most widely studied and bestunderstood animal models in biology.. Three features are key to C. elegans' success as a model organism: its invariant cell lineage, transparency, and genetic tractability.. These render it ideal for a diverse range of microscopy-based studies directly in vivo.

    Article Title: A neural tract-inspired conduit for facile, on-demand biopsy of glioblastoma
    Article Snippet: Tiled images were obtained using a Leica semi-confocal microscope fluorescent imaging system, and quantification procedures were performed for DAPI, GFP, and Ku80 antibodies using pre-defined thresholds. .. Images were processed through either Image J or MetaMorph Microscopy Image Analysis Software. ..

    Article Title: Carbene formation as a mechanism for efficient intracellular uptake of cationic antimicrobial carbon acid polymers.
    Article Snippet: .. The fluorescence images were edited by subtracting the background with MetaMorph Microscopy Automation and Image Analysis Software. ..

    Article Title: Dual regulation of IP 3 receptors by IP 3 and PIP 2 controls the transition from local to global Ca 2+ signals.
    Article Snippet: All fluorescence images and image stacks were background-corrected before analysis via subtraction of the fluorescence de- tected from a region outside the cell using MetaMorph Microscopy Automation and Image Analysis Software. .. All fluorescence images and image stacks were background-corrected before analysis via subtraction of the fluorescence de- tected from a region outside the cell using MetaMorph Microscopy Automation and Image Analysis Software. ..

    Article Title: Mutations in Parkinsonism-linked endocytic proteins synaptojanin1 and auxilin have synergistic effects on dopaminergic axonal pathology.
    Article Snippet: .. Image acquisition involved tiling of the entire section using a 20x Air lens, keeping the same laser intensity and exposure for both channels on MetaMorph Microscopy Automation and Image Analysis Software (RRID: SCR_002368). .. Stitched images were then set to the same threshold intensity for each individual channel in Fiji software (Version ImageJ 1.52p/Java 1.8.0_172 (64-bit), RRID: SCR_002285).

    Article Title: Development of Bifunctional, Raman Active Diyne-Girder Stapled α-Helical Peptides.
    Article Snippet: Stapled peptides are a unique class of cyclic αhelical peptides that are conformationally constrained via their amino acid side-chains.. They have been transformative to the field of chemical biology and peptide drug discovery through addressing many of the physicochemical limitations of linear peptides.. However, there are several issues with current chemical strategies to produce stapled peptides.

    other:

    Article Title: Probing DNA damage sites reveals context-dependent and novel DNA damage response factors
    Article Snippet: Spinning disk z-stacks were acquired using MetaMorph Microscopy Automation & Image Analysis Software.

    Article Title: Dynamin1 long- and short-tail isoforms exploit distinct recruitment and spatial patterns to form endocytic nanoclusters
    Article Snippet: Data Policy information about availability of data All manuscripts must include a data availability statement This statement should provide the following information, where applicable: - Accession codes, unique identifiers, or web links for publicly available datasets - A description of any restrictions on data availability - For clinical datasets or third party data, please ensure that the statement adheres to our policy Frédéric A. Meunier and Merja Joensuu 2 April 2024 TIRF microscopy and super resolution microscopy data (sptPALM) were acquired using Metamorph Microscopy Automation and Image analysis Software, version 7.7.8.

    Analysis:

    Article Title: A neural tract-inspired conduit for facile, on-demand biopsy of glioblastoma
    Article Snippet: Tiled images were obtained using a Leica semi-confocal microscope fluorescent imaging system, and quantification procedures were performed for DAPI, GFP, and Ku80 antibodies using pre-defined thresholds. .. Images were processed through either Image J or MetaMorph Microscopy Image Analysis Software. ..

    Fluorescence:

    Article Title: Carbene formation as a mechanism for efficient intracellular uptake of cationic antimicrobial carbon acid polymers.
    Article Snippet: .. The fluorescence images were edited by subtracting the background with MetaMorph Microscopy Automation and Image Analysis Software. ..

    Article Title: Dual regulation of IP 3 receptors by IP 3 and PIP 2 controls the transition from local to global Ca 2+ signals.
    Article Snippet: All fluorescence images and image stacks were background-corrected before analysis via subtraction of the fluorescence de- tected from a region outside the cell using MetaMorph Microscopy Automation and Image Analysis Software. .. All fluorescence images and image stacks were background-corrected before analysis via subtraction of the fluorescence de- tected from a region outside the cell using MetaMorph Microscopy Automation and Image Analysis Software. ..



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    Ablating Pcbp1 in β cells upregulates PCBP2 and does not alter glucose homeostasis. (A) Model of Cre-mediated recombination of Pcbp1 . The top diagram represents the Pcbp1 locus (light boxes represent UTRs, dark box represents protein coding region, and arrow represents transcriptional start site) with inserted loxP sites (triangles). Bottom diagram represents the Pcbp1 locus following RIP-Cre mediated recombination and remaining loxP site (triangle). (B) Western blot showing PCBP1 levels in islets from 26 week old male Pcbp1 βKO mice ( Pcbp1 Fl/Fl ,n = 3; Pcbp1 βKO ,n = 3). (C) <t>Co-immunofluorescence</t> staining for PCBP1 and Insulin in adult islets from 7 week old male Pcbp1 Fl/Fl and Pcbp1 βKO mice (scale bar, 50 μm). (D) Intraperitoneal glucose tolerance tests performed on 8–11 week old male mice (n = 9 Pcbp1 Fl/Fl and n = 11 Pcbp1 βKO ). (E) Western blots showing PCBP2 levels in islets from 26 week old male Pcbp1 Fl/Fl and Pcbp1 βKO mice ( Pcbp1 Fl/Fl , n = 3; Pcbp1 βKO , n = 3). ∗P-value<0.05, ∗∗P-value<0.01 by student's t-test.
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    Image Search Results


    Ablating Pcbp1 in β cells upregulates PCBP2 and does not alter glucose homeostasis. (A) Model of Cre-mediated recombination of Pcbp1 . The top diagram represents the Pcbp1 locus (light boxes represent UTRs, dark box represents protein coding region, and arrow represents transcriptional start site) with inserted loxP sites (triangles). Bottom diagram represents the Pcbp1 locus following RIP-Cre mediated recombination and remaining loxP site (triangle). (B) Western blot showing PCBP1 levels in islets from 26 week old male Pcbp1 βKO mice ( Pcbp1 Fl/Fl ,n = 3; Pcbp1 βKO ,n = 3). (C) Co-immunofluorescence staining for PCBP1 and Insulin in adult islets from 7 week old male Pcbp1 Fl/Fl and Pcbp1 βKO mice (scale bar, 50 μm). (D) Intraperitoneal glucose tolerance tests performed on 8–11 week old male mice (n = 9 Pcbp1 Fl/Fl and n = 11 Pcbp1 βKO ). (E) Western blots showing PCBP2 levels in islets from 26 week old male Pcbp1 Fl/Fl and Pcbp1 βKO mice ( Pcbp1 Fl/Fl , n = 3; Pcbp1 βKO , n = 3). ∗P-value<0.05, ∗∗P-value<0.01 by student's t-test.

    Journal: Molecular Metabolism

    Article Title: RNA binding proteins PCBP1 and PCBP2 regulate pancreatic β cell translation

    doi: 10.1016/j.molmet.2025.102175

    Figure Lengend Snippet: Ablating Pcbp1 in β cells upregulates PCBP2 and does not alter glucose homeostasis. (A) Model of Cre-mediated recombination of Pcbp1 . The top diagram represents the Pcbp1 locus (light boxes represent UTRs, dark box represents protein coding region, and arrow represents transcriptional start site) with inserted loxP sites (triangles). Bottom diagram represents the Pcbp1 locus following RIP-Cre mediated recombination and remaining loxP site (triangle). (B) Western blot showing PCBP1 levels in islets from 26 week old male Pcbp1 βKO mice ( Pcbp1 Fl/Fl ,n = 3; Pcbp1 βKO ,n = 3). (C) Co-immunofluorescence staining for PCBP1 and Insulin in adult islets from 7 week old male Pcbp1 Fl/Fl and Pcbp1 βKO mice (scale bar, 50 μm). (D) Intraperitoneal glucose tolerance tests performed on 8–11 week old male mice (n = 9 Pcbp1 Fl/Fl and n = 11 Pcbp1 βKO ). (E) Western blots showing PCBP2 levels in islets from 26 week old male Pcbp1 Fl/Fl and Pcbp1 βKO mice ( Pcbp1 Fl/Fl , n = 3; Pcbp1 βKO , n = 3). ∗P-value<0.05, ∗∗P-value<0.01 by student's t-test.

    Article Snippet: Immunofluorescence images were visualized and captured using a Nikon Eclipse E600 microscope equipped with a QImaging Q/Click digital camera and Metamorph software.

    Techniques: Western Blot, Immunofluorescence, Staining

    Single cell RNA sequencing of Pcbp1 and Pcbp2 co-depleted β cells reveals dysregulation of a translational mRNA program. (A) Co-immunofluorescence staining for PCBP1 and PCBP2 in adult islets from 11–12 week old male Pcbp1/2 Fl/Fl and Pcbp1/2 βKO mice (scale bar, 50 μm). Yellow arrows denote PCBP1 and PCBP2 co-depleted β cells. (B) Merged uniform manifold approximation and projection (UMAP) plot showing 3,592 control β cells (red) and 1,554 Pcbp1/2 co-depleted β cells (blue). (C) UMAP plot showing identified β cell subclusters in control and mutant β cells. (D) Dotplot showing the relative expression of Ins1 , Pcbp1 , and Pcbp2 transcript levels in each identified β cell subcluster. (E) Volcano plot of genes with significantly downregulated (blue) and upregulated (red) transcript levels in cluster 11 as compared to control β cells. (F) Reactome pathway analysis of the genes with altered mRNA levels in the setting of Pcbp1/2 co-deficiency.

    Journal: Molecular Metabolism

    Article Title: RNA binding proteins PCBP1 and PCBP2 regulate pancreatic β cell translation

    doi: 10.1016/j.molmet.2025.102175

    Figure Lengend Snippet: Single cell RNA sequencing of Pcbp1 and Pcbp2 co-depleted β cells reveals dysregulation of a translational mRNA program. (A) Co-immunofluorescence staining for PCBP1 and PCBP2 in adult islets from 11–12 week old male Pcbp1/2 Fl/Fl and Pcbp1/2 βKO mice (scale bar, 50 μm). Yellow arrows denote PCBP1 and PCBP2 co-depleted β cells. (B) Merged uniform manifold approximation and projection (UMAP) plot showing 3,592 control β cells (red) and 1,554 Pcbp1/2 co-depleted β cells (blue). (C) UMAP plot showing identified β cell subclusters in control and mutant β cells. (D) Dotplot showing the relative expression of Ins1 , Pcbp1 , and Pcbp2 transcript levels in each identified β cell subcluster. (E) Volcano plot of genes with significantly downregulated (blue) and upregulated (red) transcript levels in cluster 11 as compared to control β cells. (F) Reactome pathway analysis of the genes with altered mRNA levels in the setting of Pcbp1/2 co-deficiency.

    Article Snippet: Immunofluorescence images were visualized and captured using a Nikon Eclipse E600 microscope equipped with a QImaging Q/Click digital camera and Metamorph software.

    Techniques: RNA Sequencing, Immunofluorescence, Staining, Control, Mutagenesis, Expressing