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MetaMorph Inc metamorph software analysis
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Western Blot:

Article Title: The RhoA dependent anti-metastatic function of RKIP in breast cancer
Article Snippet: .. Numbers are shown for quantified phosphorylated ERK1 or ERK2 bands normalized with total ERK protein. ( b ) (Top left panel) Representative western blots of lysates prepared from control knockdown (shGFP), ERK1 (shERK1) or ERK2 (shERK2) BT20 cells. (Bottom left panel) Representative images of immunofluorescent staining with E-cad Ab and DRAQ5 of the same set of BT20 cells shown in the left top panel. (Right panel) E-cad intensity quantification on cell–cell junctions of immunofluorescent images shown in the left bottom panels using MetaMorph analysis software. ( c ) (Left panel) Representative images of immunofluorescent staining with E-cad Ab and DRAQ5 of BT20 cells with different combinations of control knockdown, RKIP knockdown (shRKIP) or ERK2 knockdown (shERK2). right) E-cad intensity quantification on cell–cell junctions of immunofluorescent images shown in the left bottom panels using MetaMorph analysis software. ( d ) (Left panel) Representative results of invasion assays with same set of cells used in ( c ) showing number of invaded cells through Matrigel in each indicated BT20 cell lines (mean ± SE). (Right panel) Representative images of the stained invaded cells in indicated cell lines shown at the left panel. ..

Article Title: The RhoA dependent anti-metastatic function of RKIP in breast cancer
Article Snippet: .. Numbers are shown for quantified active VAV2 bands normalized with total VAV2 protein. ( b ) (Left panel) Representative western blots of lysates prepared from control knockdown (shCTR), or five different VAV2 knockdowns (shVAV2 2.1–2.5 ) BT20 cells. (Middle panel) Representative images of immunofluorescent staining with E-cad Ab and DRAQ5 of the same set of BT20 cells shown in the left top panel. (Right panel) E-cad intensity quantification on cell–cell junctions of immunofluorescent images shown in the left bottom panels using MetaMorph analysis software. ( c ) (Left panel) Representative western blots of lysates prepared from control knockdown (shCTR), or two different GEF-H1 knockdowns (shGEF-H1 2.2–2.3 ) BT20 cells. (Middle panel) Representative images of immunofluorescent staining with E-cad Ab and DRAQ5 of the same set of BT20 cells shown in the left top panel. (Right panel) E-cad intensity quantification on cell–cell junctions of immunofluorescent images shown in the left bottom panels using MetaMorph analysis software. ( d ) (Top left panel) Representative images of immunofluorescent staining with E-Cad Ab and DRAQ5 of BT20 cells with different combinations of control knockdown, GEF-H1 knockdown (shGEF-H1), empty vector control (EVC) or RKIP expression. (Bottom left panel) E-cad intensity quantification on cell–cell junctions of immunofluorescent images shown in the left bottom panels using MetaMorph analysis software. (Right panel) Representative western blots of lysates prepared from the same set of cells shown in the left panel. ( e ) (Left panel) Representative results of invasion assays with same set of cells used in ( d ) showing number of invaded cells through Matrigel in each indicated BT20 cell lines (mean ± SE). (Right panel) Representative images of the stained invaded cells in indicated cell lines shown at the left panel. ..

Control:

Article Title: The RhoA dependent anti-metastatic function of RKIP in breast cancer
Article Snippet: .. Numbers are shown for quantified phosphorylated ERK1 or ERK2 bands normalized with total ERK protein. ( b ) (Top left panel) Representative western blots of lysates prepared from control knockdown (shGFP), ERK1 (shERK1) or ERK2 (shERK2) BT20 cells. (Bottom left panel) Representative images of immunofluorescent staining with E-cad Ab and DRAQ5 of the same set of BT20 cells shown in the left top panel. (Right panel) E-cad intensity quantification on cell–cell junctions of immunofluorescent images shown in the left bottom panels using MetaMorph analysis software. ( c ) (Left panel) Representative images of immunofluorescent staining with E-cad Ab and DRAQ5 of BT20 cells with different combinations of control knockdown, RKIP knockdown (shRKIP) or ERK2 knockdown (shERK2). right) E-cad intensity quantification on cell–cell junctions of immunofluorescent images shown in the left bottom panels using MetaMorph analysis software. ( d ) (Left panel) Representative results of invasion assays with same set of cells used in ( c ) showing number of invaded cells through Matrigel in each indicated BT20 cell lines (mean ± SE). (Right panel) Representative images of the stained invaded cells in indicated cell lines shown at the left panel. ..

Article Title: The RhoA dependent anti-metastatic function of RKIP in breast cancer
Article Snippet: Figure 3 RKIP-mediated inhibition of cell invasion depends on the E-cadherin (E-cad) cell–cell junction localization. ( a ) (Top left panel) Representative western blots of lysates prepared from control knockdown (shGFP), or four different E-cad knockdowns (shEcad#2–5) 4T1 cells. (Bottom left panel) Representative results of invasion assays with same set of cells showing number of invaded cells through Matrigel in each indicated 4T1 cell lines (mean ± SE). (Right panel) Representative images of the stained invaded cells in indicated cell lines shown in the bottom left panel. ( b ) (Top left panel) Representative western blots of lysates prepared from control knockdown (shGFP), or E-cad knockdowns BT20 cells. (Bottom left panel) Representative results of invasion assays with shGFP control knockdown or titrated E-cad knockdown showing number of invaded cells through Matrigel in each indicated BT20 cell lines (mean ± SE). (Right panel) Representative images of the stained invaded cells in indicated cell lines shown in the bottom left panel. ( c ) (Top left panel) Representative western blots of lysates prepared from BT20 cells with different combinations of controls, E-cad knockdown (shEcad) and RKIP expression as indicated. (Bottom left panel) Representative results of invasion assays with same set of cells showing number of invaded cells through Matrigel in each indicated BT20 cell lines (mean ± SE). (Right panel) Representative images of the stained invaded cells in indicated cell lines shown at the bottom left panel. ( d ) (Left panel) Representative western blots of lysates prepared from BT20 cells with different combinations of controls, RKIP knockdown (shRKIP 175 ) and RhoAQL expression as indicated. (Left, bottom) Representative images of immunofluorescent staining with β-Catenin Ab and DRAQ5 (Nucleus) of BT20 cells with control knockdown (shLUC), RKIP knockdown (shRKIP), empty vector control (EVC) or RKIP expression. (right panel) β-Catenin intensity quantification on cell–cell junctions of immunofluorescent images shown in the left panel using MetaMorph analysis software. .. Representative images of immunofluorescent staining with p120 Ab and DRAQ5 (Nucleus) of BT20 cells with control knockdown (shLUC), RKIP knockdown (shRKIP), empty vector control (EVC) or RKIP expression. (Right panel) p120 intensity quantification on cell–cell junctions of immunofluorescent images shown in the left panel using MetaMorph analysis software. ..

Article Title: The RhoA dependent anti-metastatic function of RKIP in breast cancer
Article Snippet: .. Numbers are shown for quantified active VAV2 bands normalized with total VAV2 protein. ( b ) (Left panel) Representative western blots of lysates prepared from control knockdown (shCTR), or five different VAV2 knockdowns (shVAV2 2.1–2.5 ) BT20 cells. (Middle panel) Representative images of immunofluorescent staining with E-cad Ab and DRAQ5 of the same set of BT20 cells shown in the left top panel. (Right panel) E-cad intensity quantification on cell–cell junctions of immunofluorescent images shown in the left bottom panels using MetaMorph analysis software. ( c ) (Left panel) Representative western blots of lysates prepared from control knockdown (shCTR), or two different GEF-H1 knockdowns (shGEF-H1 2.2–2.3 ) BT20 cells. (Middle panel) Representative images of immunofluorescent staining with E-cad Ab and DRAQ5 of the same set of BT20 cells shown in the left top panel. (Right panel) E-cad intensity quantification on cell–cell junctions of immunofluorescent images shown in the left bottom panels using MetaMorph analysis software. ( d ) (Top left panel) Representative images of immunofluorescent staining with E-Cad Ab and DRAQ5 of BT20 cells with different combinations of control knockdown, GEF-H1 knockdown (shGEF-H1), empty vector control (EVC) or RKIP expression. (Bottom left panel) E-cad intensity quantification on cell–cell junctions of immunofluorescent images shown in the left bottom panels using MetaMorph analysis software. (Right panel) Representative western blots of lysates prepared from the same set of cells shown in the left panel. ( e ) (Left panel) Representative results of invasion assays with same set of cells used in ( d ) showing number of invaded cells through Matrigel in each indicated BT20 cell lines (mean ± SE). (Right panel) Representative images of the stained invaded cells in indicated cell lines shown at the left panel. ..

Knockdown:

Article Title: The RhoA dependent anti-metastatic function of RKIP in breast cancer
Article Snippet: .. Numbers are shown for quantified phosphorylated ERK1 or ERK2 bands normalized with total ERK protein. ( b ) (Top left panel) Representative western blots of lysates prepared from control knockdown (shGFP), ERK1 (shERK1) or ERK2 (shERK2) BT20 cells. (Bottom left panel) Representative images of immunofluorescent staining with E-cad Ab and DRAQ5 of the same set of BT20 cells shown in the left top panel. (Right panel) E-cad intensity quantification on cell–cell junctions of immunofluorescent images shown in the left bottom panels using MetaMorph analysis software. ( c ) (Left panel) Representative images of immunofluorescent staining with E-cad Ab and DRAQ5 of BT20 cells with different combinations of control knockdown, RKIP knockdown (shRKIP) or ERK2 knockdown (shERK2). right) E-cad intensity quantification on cell–cell junctions of immunofluorescent images shown in the left bottom panels using MetaMorph analysis software. ( d ) (Left panel) Representative results of invasion assays with same set of cells used in ( c ) showing number of invaded cells through Matrigel in each indicated BT20 cell lines (mean ± SE). (Right panel) Representative images of the stained invaded cells in indicated cell lines shown at the left panel. ..

Article Title: The RhoA dependent anti-metastatic function of RKIP in breast cancer
Article Snippet: Figure 3 RKIP-mediated inhibition of cell invasion depends on the E-cadherin (E-cad) cell–cell junction localization. ( a ) (Top left panel) Representative western blots of lysates prepared from control knockdown (shGFP), or four different E-cad knockdowns (shEcad#2–5) 4T1 cells. (Bottom left panel) Representative results of invasion assays with same set of cells showing number of invaded cells through Matrigel in each indicated 4T1 cell lines (mean ± SE). (Right panel) Representative images of the stained invaded cells in indicated cell lines shown in the bottom left panel. ( b ) (Top left panel) Representative western blots of lysates prepared from control knockdown (shGFP), or E-cad knockdowns BT20 cells. (Bottom left panel) Representative results of invasion assays with shGFP control knockdown or titrated E-cad knockdown showing number of invaded cells through Matrigel in each indicated BT20 cell lines (mean ± SE). (Right panel) Representative images of the stained invaded cells in indicated cell lines shown in the bottom left panel. ( c ) (Top left panel) Representative western blots of lysates prepared from BT20 cells with different combinations of controls, E-cad knockdown (shEcad) and RKIP expression as indicated. (Bottom left panel) Representative results of invasion assays with same set of cells showing number of invaded cells through Matrigel in each indicated BT20 cell lines (mean ± SE). (Right panel) Representative images of the stained invaded cells in indicated cell lines shown at the bottom left panel. ( d ) (Left panel) Representative western blots of lysates prepared from BT20 cells with different combinations of controls, RKIP knockdown (shRKIP 175 ) and RhoAQL expression as indicated. (Left, bottom) Representative images of immunofluorescent staining with β-Catenin Ab and DRAQ5 (Nucleus) of BT20 cells with control knockdown (shLUC), RKIP knockdown (shRKIP), empty vector control (EVC) or RKIP expression. (right panel) β-Catenin intensity quantification on cell–cell junctions of immunofluorescent images shown in the left panel using MetaMorph analysis software. .. Representative images of immunofluorescent staining with p120 Ab and DRAQ5 (Nucleus) of BT20 cells with control knockdown (shLUC), RKIP knockdown (shRKIP), empty vector control (EVC) or RKIP expression. (Right panel) p120 intensity quantification on cell–cell junctions of immunofluorescent images shown in the left panel using MetaMorph analysis software. ..

Article Title: The RhoA dependent anti-metastatic function of RKIP in breast cancer
Article Snippet: .. Numbers are shown for quantified active VAV2 bands normalized with total VAV2 protein. ( b ) (Left panel) Representative western blots of lysates prepared from control knockdown (shCTR), or five different VAV2 knockdowns (shVAV2 2.1–2.5 ) BT20 cells. (Middle panel) Representative images of immunofluorescent staining with E-cad Ab and DRAQ5 of the same set of BT20 cells shown in the left top panel. (Right panel) E-cad intensity quantification on cell–cell junctions of immunofluorescent images shown in the left bottom panels using MetaMorph analysis software. ( c ) (Left panel) Representative western blots of lysates prepared from control knockdown (shCTR), or two different GEF-H1 knockdowns (shGEF-H1 2.2–2.3 ) BT20 cells. (Middle panel) Representative images of immunofluorescent staining with E-cad Ab and DRAQ5 of the same set of BT20 cells shown in the left top panel. (Right panel) E-cad intensity quantification on cell–cell junctions of immunofluorescent images shown in the left bottom panels using MetaMorph analysis software. ( d ) (Top left panel) Representative images of immunofluorescent staining with E-Cad Ab and DRAQ5 of BT20 cells with different combinations of control knockdown, GEF-H1 knockdown (shGEF-H1), empty vector control (EVC) or RKIP expression. (Bottom left panel) E-cad intensity quantification on cell–cell junctions of immunofluorescent images shown in the left bottom panels using MetaMorph analysis software. (Right panel) Representative western blots of lysates prepared from the same set of cells shown in the left panel. ( e ) (Left panel) Representative results of invasion assays with same set of cells used in ( d ) showing number of invaded cells through Matrigel in each indicated BT20 cell lines (mean ± SE). (Right panel) Representative images of the stained invaded cells in indicated cell lines shown at the left panel. ..

Staining:

Article Title: The RhoA dependent anti-metastatic function of RKIP in breast cancer
Article Snippet: .. Numbers are shown for quantified phosphorylated ERK1 or ERK2 bands normalized with total ERK protein. ( b ) (Top left panel) Representative western blots of lysates prepared from control knockdown (shGFP), ERK1 (shERK1) or ERK2 (shERK2) BT20 cells. (Bottom left panel) Representative images of immunofluorescent staining with E-cad Ab and DRAQ5 of the same set of BT20 cells shown in the left top panel. (Right panel) E-cad intensity quantification on cell–cell junctions of immunofluorescent images shown in the left bottom panels using MetaMorph analysis software. ( c ) (Left panel) Representative images of immunofluorescent staining with E-cad Ab and DRAQ5 of BT20 cells with different combinations of control knockdown, RKIP knockdown (shRKIP) or ERK2 knockdown (shERK2). right) E-cad intensity quantification on cell–cell junctions of immunofluorescent images shown in the left bottom panels using MetaMorph analysis software. ( d ) (Left panel) Representative results of invasion assays with same set of cells used in ( c ) showing number of invaded cells through Matrigel in each indicated BT20 cell lines (mean ± SE). (Right panel) Representative images of the stained invaded cells in indicated cell lines shown at the left panel. ..

Article Title: The RhoA dependent anti-metastatic function of RKIP in breast cancer
Article Snippet: Figure 3 RKIP-mediated inhibition of cell invasion depends on the E-cadherin (E-cad) cell–cell junction localization. ( a ) (Top left panel) Representative western blots of lysates prepared from control knockdown (shGFP), or four different E-cad knockdowns (shEcad#2–5) 4T1 cells. (Bottom left panel) Representative results of invasion assays with same set of cells showing number of invaded cells through Matrigel in each indicated 4T1 cell lines (mean ± SE). (Right panel) Representative images of the stained invaded cells in indicated cell lines shown in the bottom left panel. ( b ) (Top left panel) Representative western blots of lysates prepared from control knockdown (shGFP), or E-cad knockdowns BT20 cells. (Bottom left panel) Representative results of invasion assays with shGFP control knockdown or titrated E-cad knockdown showing number of invaded cells through Matrigel in each indicated BT20 cell lines (mean ± SE). (Right panel) Representative images of the stained invaded cells in indicated cell lines shown in the bottom left panel. ( c ) (Top left panel) Representative western blots of lysates prepared from BT20 cells with different combinations of controls, E-cad knockdown (shEcad) and RKIP expression as indicated. (Bottom left panel) Representative results of invasion assays with same set of cells showing number of invaded cells through Matrigel in each indicated BT20 cell lines (mean ± SE). (Right panel) Representative images of the stained invaded cells in indicated cell lines shown at the bottom left panel. ( d ) (Left panel) Representative western blots of lysates prepared from BT20 cells with different combinations of controls, RKIP knockdown (shRKIP 175 ) and RhoAQL expression as indicated. (Left, bottom) Representative images of immunofluorescent staining with β-Catenin Ab and DRAQ5 (Nucleus) of BT20 cells with control knockdown (shLUC), RKIP knockdown (shRKIP), empty vector control (EVC) or RKIP expression. (right panel) β-Catenin intensity quantification on cell–cell junctions of immunofluorescent images shown in the left panel using MetaMorph analysis software. .. Representative images of immunofluorescent staining with p120 Ab and DRAQ5 (Nucleus) of BT20 cells with control knockdown (shLUC), RKIP knockdown (shRKIP), empty vector control (EVC) or RKIP expression. (Right panel) p120 intensity quantification on cell–cell junctions of immunofluorescent images shown in the left panel using MetaMorph analysis software. ..

Article Title: The RhoA dependent anti-metastatic function of RKIP in breast cancer
Article Snippet: .. Numbers are shown for quantified active VAV2 bands normalized with total VAV2 protein. ( b ) (Left panel) Representative western blots of lysates prepared from control knockdown (shCTR), or five different VAV2 knockdowns (shVAV2 2.1–2.5 ) BT20 cells. (Middle panel) Representative images of immunofluorescent staining with E-cad Ab and DRAQ5 of the same set of BT20 cells shown in the left top panel. (Right panel) E-cad intensity quantification on cell–cell junctions of immunofluorescent images shown in the left bottom panels using MetaMorph analysis software. ( c ) (Left panel) Representative western blots of lysates prepared from control knockdown (shCTR), or two different GEF-H1 knockdowns (shGEF-H1 2.2–2.3 ) BT20 cells. (Middle panel) Representative images of immunofluorescent staining with E-cad Ab and DRAQ5 of the same set of BT20 cells shown in the left top panel. (Right panel) E-cad intensity quantification on cell–cell junctions of immunofluorescent images shown in the left bottom panels using MetaMorph analysis software. ( d ) (Top left panel) Representative images of immunofluorescent staining with E-Cad Ab and DRAQ5 of BT20 cells with different combinations of control knockdown, GEF-H1 knockdown (shGEF-H1), empty vector control (EVC) or RKIP expression. (Bottom left panel) E-cad intensity quantification on cell–cell junctions of immunofluorescent images shown in the left bottom panels using MetaMorph analysis software. (Right panel) Representative western blots of lysates prepared from the same set of cells shown in the left panel. ( e ) (Left panel) Representative results of invasion assays with same set of cells used in ( d ) showing number of invaded cells through Matrigel in each indicated BT20 cell lines (mean ± SE). (Right panel) Representative images of the stained invaded cells in indicated cell lines shown at the left panel. ..

Software:

Article Title: The RhoA dependent anti-metastatic function of RKIP in breast cancer
Article Snippet: .. Numbers are shown for quantified phosphorylated ERK1 or ERK2 bands normalized with total ERK protein. ( b ) (Top left panel) Representative western blots of lysates prepared from control knockdown (shGFP), ERK1 (shERK1) or ERK2 (shERK2) BT20 cells. (Bottom left panel) Representative images of immunofluorescent staining with E-cad Ab and DRAQ5 of the same set of BT20 cells shown in the left top panel. (Right panel) E-cad intensity quantification on cell–cell junctions of immunofluorescent images shown in the left bottom panels using MetaMorph analysis software. ( c ) (Left panel) Representative images of immunofluorescent staining with E-cad Ab and DRAQ5 of BT20 cells with different combinations of control knockdown, RKIP knockdown (shRKIP) or ERK2 knockdown (shERK2). right) E-cad intensity quantification on cell–cell junctions of immunofluorescent images shown in the left bottom panels using MetaMorph analysis software. ( d ) (Left panel) Representative results of invasion assays with same set of cells used in ( c ) showing number of invaded cells through Matrigel in each indicated BT20 cell lines (mean ± SE). (Right panel) Representative images of the stained invaded cells in indicated cell lines shown at the left panel. ..

Article Title: Microglia reactivity entails microtubule remodeling from acentrosomal to centrosomal arrays.
Article Snippet: .. For GM130- g-tubulin co-staining analysis, GM130 and tubulin signals frommax intensity z-projections were uniformly processed among different images increasing the ‘brightness’ and ‘contrast’ parameters by the same percentage. g-tubulin signal over cell area was calculated as percentage of cell area covered by g-tubulin signal; g-tubulin signal threshold was uniformly applied on MetaMorph analysis software by setting the same minimum values to all images. .. Two or more distinct g-tubulin+ puncta were identified by counting the peaks of fluorescence intensity on a linescan drawn through the centroid of each puncta using the free-hand tool on ImageJ; the Find Peaks ImageJ pluginwas used to identify the peaks by setting theminimumpeak amplitude value at 100 gray values.

Article Title: The RhoA dependent anti-metastatic function of RKIP in breast cancer
Article Snippet: Figure 3 RKIP-mediated inhibition of cell invasion depends on the E-cadherin (E-cad) cell–cell junction localization. ( a ) (Top left panel) Representative western blots of lysates prepared from control knockdown (shGFP), or four different E-cad knockdowns (shEcad#2–5) 4T1 cells. (Bottom left panel) Representative results of invasion assays with same set of cells showing number of invaded cells through Matrigel in each indicated 4T1 cell lines (mean ± SE). (Right panel) Representative images of the stained invaded cells in indicated cell lines shown in the bottom left panel. ( b ) (Top left panel) Representative western blots of lysates prepared from control knockdown (shGFP), or E-cad knockdowns BT20 cells. (Bottom left panel) Representative results of invasion assays with shGFP control knockdown or titrated E-cad knockdown showing number of invaded cells through Matrigel in each indicated BT20 cell lines (mean ± SE). (Right panel) Representative images of the stained invaded cells in indicated cell lines shown in the bottom left panel. ( c ) (Top left panel) Representative western blots of lysates prepared from BT20 cells with different combinations of controls, E-cad knockdown (shEcad) and RKIP expression as indicated. (Bottom left panel) Representative results of invasion assays with same set of cells showing number of invaded cells through Matrigel in each indicated BT20 cell lines (mean ± SE). (Right panel) Representative images of the stained invaded cells in indicated cell lines shown at the bottom left panel. ( d ) (Left panel) Representative western blots of lysates prepared from BT20 cells with different combinations of controls, RKIP knockdown (shRKIP 175 ) and RhoAQL expression as indicated. (Left, bottom) Representative images of immunofluorescent staining with β-Catenin Ab and DRAQ5 (Nucleus) of BT20 cells with control knockdown (shLUC), RKIP knockdown (shRKIP), empty vector control (EVC) or RKIP expression. (right panel) β-Catenin intensity quantification on cell–cell junctions of immunofluorescent images shown in the left panel using MetaMorph analysis software. .. Representative images of immunofluorescent staining with p120 Ab and DRAQ5 (Nucleus) of BT20 cells with control knockdown (shLUC), RKIP knockdown (shRKIP), empty vector control (EVC) or RKIP expression. (Right panel) p120 intensity quantification on cell–cell junctions of immunofluorescent images shown in the left panel using MetaMorph analysis software. ..

Article Title: Modulation of haemocyte motility by chemical and biological stresses in Mytilus edulis and Dreissena polymorpha.
Article Snippet: Mussels are constantly exposed to various pollutants in the environment, which can impair their immune defences against microbes and thus threaten their survival.. In this study, we expand the insight into a key parameter of immune response in two mussel species by exploring the impact of exposure to pollutants or bacteria or simultaneous chemical and biological exposure on haemocyte motility.. Basal haemocyte velocity in primary culture was high and increasing over time in Mytilus edulis (mean cell speed of 2.32 μm/min ± 1.57) whereas Dreissena polymorpha showed a constant and rather low cell motility with time (mean cell speed of 0.59 μm/min ± 0.1).

Article Title: Islet amyloid polypeptide does not suppress pancreatic cancer.
Article Snippet: .. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). www.molecularmetabolism.com 3 Original Article analyzed using MetaMorph analysis software. ..

Article Title: Genetic susceptibility to diabetic kidney disease is linked to promoter variants of XOR
Article Snippet: .. Open in a separate window Fig. 4 ∣ Xor promoter risk variants promote podocyte depletion and glomerular injury with diabetes. a , b , Representative histopathology images of diabetic mice: B6- Xor WT + STZ ( n = 6) ( a ), B6- Xor em1 + STZ ( n = 8) ( b ), D2-WT + STZ ( n = 5) ( c ) (PAS; ×40 magnification; scale bars, 100 μm). d , The sclerosis index score from diabetic mice in a – c : 0 represents no lesion, 1+ represents sclerosis of <25% of the glomerulus; 2+, 3+, and 4+ represent sclerosis of >25% to 50%, >50% to 75%, and >75% of the glomerulus, respectively. e , The glomerular surface area (GSA in μm 2 ) was calculated in diabetic mice in a – c using MetaMorph analysis software. ..

Article Title: The RhoA dependent anti-metastatic function of RKIP in breast cancer
Article Snippet: .. Numbers are shown for quantified active VAV2 bands normalized with total VAV2 protein. ( b ) (Left panel) Representative western blots of lysates prepared from control knockdown (shCTR), or five different VAV2 knockdowns (shVAV2 2.1–2.5 ) BT20 cells. (Middle panel) Representative images of immunofluorescent staining with E-cad Ab and DRAQ5 of the same set of BT20 cells shown in the left top panel. (Right panel) E-cad intensity quantification on cell–cell junctions of immunofluorescent images shown in the left bottom panels using MetaMorph analysis software. ( c ) (Left panel) Representative western blots of lysates prepared from control knockdown (shCTR), or two different GEF-H1 knockdowns (shGEF-H1 2.2–2.3 ) BT20 cells. (Middle panel) Representative images of immunofluorescent staining with E-cad Ab and DRAQ5 of the same set of BT20 cells shown in the left top panel. (Right panel) E-cad intensity quantification on cell–cell junctions of immunofluorescent images shown in the left bottom panels using MetaMorph analysis software. ( d ) (Top left panel) Representative images of immunofluorescent staining with E-Cad Ab and DRAQ5 of BT20 cells with different combinations of control knockdown, GEF-H1 knockdown (shGEF-H1), empty vector control (EVC) or RKIP expression. (Bottom left panel) E-cad intensity quantification on cell–cell junctions of immunofluorescent images shown in the left bottom panels using MetaMorph analysis software. (Right panel) Representative western blots of lysates prepared from the same set of cells shown in the left panel. ( e ) (Left panel) Representative results of invasion assays with same set of cells used in ( d ) showing number of invaded cells through Matrigel in each indicated BT20 cell lines (mean ± SE). (Right panel) Representative images of the stained invaded cells in indicated cell lines shown at the left panel. ..

Expressing:

Article Title: The RhoA dependent anti-metastatic function of RKIP in breast cancer
Article Snippet: Figure 3 RKIP-mediated inhibition of cell invasion depends on the E-cadherin (E-cad) cell–cell junction localization. ( a ) (Top left panel) Representative western blots of lysates prepared from control knockdown (shGFP), or four different E-cad knockdowns (shEcad#2–5) 4T1 cells. (Bottom left panel) Representative results of invasion assays with same set of cells showing number of invaded cells through Matrigel in each indicated 4T1 cell lines (mean ± SE). (Right panel) Representative images of the stained invaded cells in indicated cell lines shown in the bottom left panel. ( b ) (Top left panel) Representative western blots of lysates prepared from control knockdown (shGFP), or E-cad knockdowns BT20 cells. (Bottom left panel) Representative results of invasion assays with shGFP control knockdown or titrated E-cad knockdown showing number of invaded cells through Matrigel in each indicated BT20 cell lines (mean ± SE). (Right panel) Representative images of the stained invaded cells in indicated cell lines shown in the bottom left panel. ( c ) (Top left panel) Representative western blots of lysates prepared from BT20 cells with different combinations of controls, E-cad knockdown (shEcad) and RKIP expression as indicated. (Bottom left panel) Representative results of invasion assays with same set of cells showing number of invaded cells through Matrigel in each indicated BT20 cell lines (mean ± SE). (Right panel) Representative images of the stained invaded cells in indicated cell lines shown at the bottom left panel. ( d ) (Left panel) Representative western blots of lysates prepared from BT20 cells with different combinations of controls, RKIP knockdown (shRKIP 175 ) and RhoAQL expression as indicated. (Left, bottom) Representative images of immunofluorescent staining with β-Catenin Ab and DRAQ5 (Nucleus) of BT20 cells with control knockdown (shLUC), RKIP knockdown (shRKIP), empty vector control (EVC) or RKIP expression. (right panel) β-Catenin intensity quantification on cell–cell junctions of immunofluorescent images shown in the left panel using MetaMorph analysis software. .. Representative images of immunofluorescent staining with p120 Ab and DRAQ5 (Nucleus) of BT20 cells with control knockdown (shLUC), RKIP knockdown (shRKIP), empty vector control (EVC) or RKIP expression. (Right panel) p120 intensity quantification on cell–cell junctions of immunofluorescent images shown in the left panel using MetaMorph analysis software. ..

Article Title: The RhoA dependent anti-metastatic function of RKIP in breast cancer
Article Snippet: .. Numbers are shown for quantified active VAV2 bands normalized with total VAV2 protein. ( b ) (Left panel) Representative western blots of lysates prepared from control knockdown (shCTR), or five different VAV2 knockdowns (shVAV2 2.1–2.5 ) BT20 cells. (Middle panel) Representative images of immunofluorescent staining with E-cad Ab and DRAQ5 of the same set of BT20 cells shown in the left top panel. (Right panel) E-cad intensity quantification on cell–cell junctions of immunofluorescent images shown in the left bottom panels using MetaMorph analysis software. ( c ) (Left panel) Representative western blots of lysates prepared from control knockdown (shCTR), or two different GEF-H1 knockdowns (shGEF-H1 2.2–2.3 ) BT20 cells. (Middle panel) Representative images of immunofluorescent staining with E-cad Ab and DRAQ5 of the same set of BT20 cells shown in the left top panel. (Right panel) E-cad intensity quantification on cell–cell junctions of immunofluorescent images shown in the left bottom panels using MetaMorph analysis software. ( d ) (Top left panel) Representative images of immunofluorescent staining with E-Cad Ab and DRAQ5 of BT20 cells with different combinations of control knockdown, GEF-H1 knockdown (shGEF-H1), empty vector control (EVC) or RKIP expression. (Bottom left panel) E-cad intensity quantification on cell–cell junctions of immunofluorescent images shown in the left bottom panels using MetaMorph analysis software. (Right panel) Representative western blots of lysates prepared from the same set of cells shown in the left panel. ( e ) (Left panel) Representative results of invasion assays with same set of cells used in ( d ) showing number of invaded cells through Matrigel in each indicated BT20 cell lines (mean ± SE). (Right panel) Representative images of the stained invaded cells in indicated cell lines shown at the left panel. ..

other:

Article Title: RadD is a RecA-dependent accessory protein that accelerates DNA strand exchange
Article Snippet: Each molecule was measured three times using Metamorph analysis software and the average length was calculated in nm.

Histopathology:

Article Title: Genetic susceptibility to diabetic kidney disease is linked to promoter variants of XOR
Article Snippet: .. Open in a separate window Fig. 4 ∣ Xor promoter risk variants promote podocyte depletion and glomerular injury with diabetes. a , b , Representative histopathology images of diabetic mice: B6- Xor WT + STZ ( n = 6) ( a ), B6- Xor em1 + STZ ( n = 8) ( b ), D2-WT + STZ ( n = 5) ( c ) (PAS; ×40 magnification; scale bars, 100 μm). d , The sclerosis index score from diabetic mice in a – c : 0 represents no lesion, 1+ represents sclerosis of <25% of the glomerulus; 2+, 3+, and 4+ represent sclerosis of >25% to 50%, >50% to 75%, and >75% of the glomerulus, respectively. e , The glomerular surface area (GSA in μm 2 ) was calculated in diabetic mice in a – c using MetaMorph analysis software. ..



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