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messageclean kit  (GenHunter Corporation)

 
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    Structured Review

    GenHunter Corporation messageclean kit
    Messageclean Kit, supplied by GenHunter Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/messageclean+kit/messageclean+kit/pmc05872329-88-9-11
    Average 90 stars, based on 1 article reviews
    messageclean kit - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Purification:

    Article Title: Isolation and characterization of a new DUR3-like gene, PyDUR3.3, from the marine macroalga Pyropia yezoensis (Rhodophyta)
    Article Snippet: Total RNA extraction from fresh P. yezoensis thalli and conchocelis was performed using the RNeasy Plant Mini Kit (Qiagen, Hilden, Germany). .. After DNase treatment using the MessageClean Kit (GenHunter, Tennessee, USA), the purified total RNA was stored at −80 °C until singlestranded (ss) cDNA synthesis for 3′ or 5′ rapid amplification of cDNA ends (RACE) and quantitative-polymerase chain reaction (qPCR) was performed. ..

    Article Title: Isolation and functional characterization of an ammonium transporter gene, PyAMT1, related to nitrogen assimilation in the marine macroalga Pyropia yezoensis (Rhodophyta).
    Article Snippet: Ammonium and nitrate are the primary nitrogen sources in natural environments, and are essential for growth and development in photosynthetic eukaryotes.. In this study, we report on the isolation and characterization of an ammonium transporter gene (PyAMT1) which performs a key function in nitrogen (N) metabolism of Pyropia yezoensis thalli.. The predicted length of PyAMT1 was 483 amino acids (AAs).

    cDNA Synthesis:

    Article Title: Isolation and characterization of a new DUR3-like gene, PyDUR3.3, from the marine macroalga Pyropia yezoensis (Rhodophyta)
    Article Snippet: Total RNA extraction from fresh P. yezoensis thalli and conchocelis was performed using the RNeasy Plant Mini Kit (Qiagen, Hilden, Germany). .. After DNase treatment using the MessageClean Kit (GenHunter, Tennessee, USA), the purified total RNA was stored at −80 °C until singlestranded (ss) cDNA synthesis for 3′ or 5′ rapid amplification of cDNA ends (RACE) and quantitative-polymerase chain reaction (qPCR) was performed. ..

    Article Title: Isolation and functional characterization of an ammonium transporter gene, PyAMT1, related to nitrogen assimilation in the marine macroalga Pyropia yezoensis (Rhodophyta).
    Article Snippet: Ammonium and nitrate are the primary nitrogen sources in natural environments, and are essential for growth and development in photosynthetic eukaryotes.. In this study, we report on the isolation and characterization of an ammonium transporter gene (PyAMT1) which performs a key function in nitrogen (N) metabolism of Pyropia yezoensis thalli.. The predicted length of PyAMT1 was 483 amino acids (AAs).

    Rapid Amplification of cDNA Ends:

    Article Title: Isolation and characterization of a new DUR3-like gene, PyDUR3.3, from the marine macroalga Pyropia yezoensis (Rhodophyta)
    Article Snippet: Total RNA extraction from fresh P. yezoensis thalli and conchocelis was performed using the RNeasy Plant Mini Kit (Qiagen, Hilden, Germany). .. After DNase treatment using the MessageClean Kit (GenHunter, Tennessee, USA), the purified total RNA was stored at −80 °C until singlestranded (ss) cDNA synthesis for 3′ or 5′ rapid amplification of cDNA ends (RACE) and quantitative-polymerase chain reaction (qPCR) was performed. ..

    Article Title: Isolation and functional characterization of an ammonium transporter gene, PyAMT1, related to nitrogen assimilation in the marine macroalga Pyropia yezoensis (Rhodophyta).
    Article Snippet: Ammonium and nitrate are the primary nitrogen sources in natural environments, and are essential for growth and development in photosynthetic eukaryotes.. In this study, we report on the isolation and characterization of an ammonium transporter gene (PyAMT1) which performs a key function in nitrogen (N) metabolism of Pyropia yezoensis thalli.. The predicted length of PyAMT1 was 483 amino acids (AAs).

    Real-time Polymerase Chain Reaction:

    Article Title: Isolation and characterization of a new DUR3-like gene, PyDUR3.3, from the marine macroalga Pyropia yezoensis (Rhodophyta)
    Article Snippet: Total RNA extraction from fresh P. yezoensis thalli and conchocelis was performed using the RNeasy Plant Mini Kit (Qiagen, Hilden, Germany). .. After DNase treatment using the MessageClean Kit (GenHunter, Tennessee, USA), the purified total RNA was stored at −80 °C until singlestranded (ss) cDNA synthesis for 3′ or 5′ rapid amplification of cDNA ends (RACE) and quantitative-polymerase chain reaction (qPCR) was performed. ..

    Article Title: Isolation and functional characterization of an ammonium transporter gene, PyAMT1, related to nitrogen assimilation in the marine macroalga Pyropia yezoensis (Rhodophyta).
    Article Snippet: Ammonium and nitrate are the primary nitrogen sources in natural environments, and are essential for growth and development in photosynthetic eukaryotes.. In this study, we report on the isolation and characterization of an ammonium transporter gene (PyAMT1) which performs a key function in nitrogen (N) metabolism of Pyropia yezoensis thalli.. The predicted length of PyAMT1 was 483 amino acids (AAs).

    Article Title: Differences in Sirtuin Regulation in Response to Calorie Restriction in Cryptococcus neoformans
    Article Snippet: Briefly, RNA was extracted with the RNeasy mini kit (Qiagen, Hilden, Germany) per manufacturer’s instructions, with an initial step of mechanical disruption using a mini bead beater (Biospec, Bartlesville, OK, USA). .. RNA was then cleaned for DNA contamination using the MessageClean kit (GenHunter Corp., Nashville, TN, USA), and then real-time PCR was performed using the Power SYBR Green RNA-to-C T 1-Step kit (Applied Biosystems, Foster City, CA, USA) per the manufacturer’s instructions, and using primers listed in . ..

    Isolation:

    Article Title: Cone dystrophy and ectopic synaptogenesis in a Cacna1f loss of function model of congenital stationary night blindness (CSNB2A)
    Article Snippet: .. Total RNA was isolated from retinas of adult (3-5 months) C57BL/6J wildtype and G305X mice age-matched littermate mice, using Trizol Reagent (Invitrogen, https://www.thermofisher.com/order/catalog/product/15596026 ), and treated with the MessageClean kit (GenHunter, http://www.genhunter.com/products/messageclean-kit-rna-cleaning.html ) to remove contaminating DNA. .. RNA from 10 adult animals of each genotype was combined to reduce effects of individual variation. cDNA was then synthesized, coupled to Cy3 or Cy5 dye, and purified, using the Superscript Indirect cDNA Labeling System (Invitrogen, https://www.thermofisher.com/order/catalog/product/L101402 ).

    Synthesized:

    Article Title: Modulation of Replicative Lifespan in Cryptococcus neoformans : Implications for Virulence
    Article Snippet: .. The RNA was cleaned for DNA contamination using the MessageClean kit (GenHunter, Corp.), and cDNA was synthesized using the First-strand Superscript II RT kit (Life Technologies) according to the manufacturer’s instructions. .. Relative expression of genes was measured by real-time PCR using SYBR green (Applied Biosystems) in an ABI 96 system using primers listed in Supplementary Table .

    SYBR Green Assay:

    Article Title: Differences in Sirtuin Regulation in Response to Calorie Restriction in Cryptococcus neoformans
    Article Snippet: Briefly, RNA was extracted with the RNeasy mini kit (Qiagen, Hilden, Germany) per manufacturer’s instructions, with an initial step of mechanical disruption using a mini bead beater (Biospec, Bartlesville, OK, USA). .. RNA was then cleaned for DNA contamination using the MessageClean kit (GenHunter Corp., Nashville, TN, USA), and then real-time PCR was performed using the Power SYBR Green RNA-to-C T 1-Step kit (Applied Biosystems, Foster City, CA, USA) per the manufacturer’s instructions, and using primers listed in . ..



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