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mega7 0 molecular evolutionary genetic analysis  (Percival Scientific)

 
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    Percival Scientific mega7 0 molecular evolutionary genetic analysis
    Mega7 0 Molecular Evolutionary Genetic Analysis, supplied by Percival Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mega+7+program/pm37421622-213-163-184
    Average 86 stars, based on 1 article reviews
    mega7 0 molecular evolutionary genetic analysis - by Bioz Stars, 2026-11
    86/100 stars

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    RNA Sequencing:

    Article Title: OsTOC1 plays dual roles in the regulation of plant circadian clock by functioning as a direct transcription activator or repressor.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies GFP Polyclonal Antibody Invitrogen Cat # A-11122; RRID: AB_221569 Actin (26F7) mAb for PLANTs Abmart Cat #M20009; RRID: AB_2936239 Anti-Histone H3 antibody Abcam Cat # ab1791; RRID: AB_302613 Goat anti-Rabbit IgG (H + L) Secondary Antibody, HRP Invitrogen Cat # 31460; RRID: AB_228341 Anti-mouse IgG, HRP-linked Antibody Cell Signaling Technology Cat # 7076; RRID; AB_330924 Bacterial and virus strains Escherichia coli strain DH10B N/A N/A Escherichia coli strain BL21(AI) Weidi Cat # EC1004 Escherichia coli strain NS3529 N/A N/A Agrobacterium tumefaciens GV3101(pSoup-p19) Weidi Cat # AC1003 Xanthomonas oryzae pv. oryzae N/A N/A Agrobacterium tumefaciens EHA105 Weidi Cat # AC1010 Chemicals, peptides, and recombinant proteins TRIzolTM reagent Invitrogen Cat # 15596026 Dexamethasone Sigma-Aldrich Cat #D4902 Cyclohexane Sigma-Aldrich Cat #C698 Dimethyl sulfoxide Sigma-Aldrich Cat #D2650 D-Luciferin, Potassium Salt GOLDBIO Cat # 010637LUCK X-Gluc GOLDBIO Cat #G1281C1 DAPI BBI Cat #E607303 Protease Inhibitor Cocktails Roche Cat # 11873580001 PMSF Roche Cat # 10837091001 Nonidet-P40 Roche Cat # 14883100 Poly dI dC Sigma-Aldrich Cat #P0913 PhytagelTM Sigma-Aldrich Cat #P8169-500G Protein A beads New England Biolabs Cat #S1425 Cellulase R10 Yakult Japan Cat # 180612-02 Maecrozyme R10 Yakult Japan Cat # 171208-02 T5 Exonuclease Epicentre Cat #T5E4111K DNA Polymerase New England Biolabs Cat #M0535S Taq DNA Ligase New England Biolabs Cat #M0208L GST-OsTOC1 This paper N/A Critical commercial assays ReverTre Ace Qpcr RT Master Mix TOYOBO Cat # FSQ-301 23SYB Mix Transgen Cat # AQ601-01 Dual-Luciferase Reporter Assay System Promega Cat #E1500 QubitTM dsDNA HS Assay Invitrogen Cat #Q32851 NEBNext UltraTM II DNA Library Prep Kit for Illumina New England Biolabs Cat #E7645S NEBNext Multiplex Oligos for Illumina New England Biolabs Cat #E7335 KOD FX TOYOBO Cat # KFX-101 Kimura B nutrient salts Solution Coolaber Cat # NS1050-2000L Chemiluminescent Detection Module *Component of DAFA Kits Thermo Fisher Scientific Cat # 89880 (Continued on next page) 14 Cell Reports 42, 112765, July 25, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Raw RNA-seq data https://ngdc.cncb.ac.cn PRJCA016700 Raw ChIP-seq data https://ngdc.cncb.ac.cn PRJCA016659 Experimental models: Organisms/strains Oryza sativa: OsTOC1-ox This paper N/A Oryza sativa: OsTOC1-RNAi This paper N/A Oryza sativa: Ostoc1 This paper N/A Oryza sativa: OsTOC1-GFP-GR This paper N/A Oryza sativa: OsTMG This paper N/A Oryza sativa: pOsTOC1:GUS This paper N/A Nicotiana benthamiana This paper N/A Oligonucleotides See Table S2 This paper N/A Recombinant DNA pUbi:OsTOC1 GFP/pCABR1:LUC This paper N/A CRISPR/Cas9 OsTOC1/pCABR1:LUC This paper N/A pUbi:OsTOC1-GFP-GR This paper N/A pOsTOC1:GUS This paper N/A pOsTOC1:OsTOC1-GFP This paper N/A pUbi:OsTOC1-antiOsTOC1 This paper N/A pGEX 4T-1 GST-OsTOC1 This paper N/A pGreen0800 62-SK-OsTOC1 This paper N/A 35s:GAL4BD-VP16-OsTOC1 This paper N/A 35s:GAL4BD-VP16-AtTOC1 This paper N/A pGreen0800 pOsTGAL3a:LUC This paper N/A pGreen0800 pOsTGAL3b:LUC This paper N/A pGreen0800 pmOsTGAL3a:LUC(mOsTBM) This paper N/A pGreen0800 pOsIAA12:LUC This paper N/A pGreen0800 pOsIAA27:LUC This paper N/A pGreen0800 pOsGI:LUC This paper N/A pGreen0800 pOsLHY:LUC This paper N/A Software and algorithms ImageJ Schneider et al.7 https://imagej.nih.gov/ij/ Graphpad grism GraphPad Software www.graphpad.com MEGA7.0 Molecular Evolutionary Genetic Analysis https://www.megasoftware.net/ IGV 2.12.0 Integrative Genomics Viewer https://software.broadinstitute.org/software/igv Other Confocal microscope ZEISS LSM 7810 Plant growth chamber Percival Scientific E 41L2 Microplate luminometer Berthold Tristar2 S LB 942 ..

    Chromatin Immunoprecipitation:

    Article Title: OsTOC1 plays dual roles in the regulation of plant circadian clock by functioning as a direct transcription activator or repressor.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies GFP Polyclonal Antibody Invitrogen Cat # A-11122; RRID: AB_221569 Actin (26F7) mAb for PLANTs Abmart Cat #M20009; RRID: AB_2936239 Anti-Histone H3 antibody Abcam Cat # ab1791; RRID: AB_302613 Goat anti-Rabbit IgG (H + L) Secondary Antibody, HRP Invitrogen Cat # 31460; RRID: AB_228341 Anti-mouse IgG, HRP-linked Antibody Cell Signaling Technology Cat # 7076; RRID; AB_330924 Bacterial and virus strains Escherichia coli strain DH10B N/A N/A Escherichia coli strain BL21(AI) Weidi Cat # EC1004 Escherichia coli strain NS3529 N/A N/A Agrobacterium tumefaciens GV3101(pSoup-p19) Weidi Cat # AC1003 Xanthomonas oryzae pv. oryzae N/A N/A Agrobacterium tumefaciens EHA105 Weidi Cat # AC1010 Chemicals, peptides, and recombinant proteins TRIzolTM reagent Invitrogen Cat # 15596026 Dexamethasone Sigma-Aldrich Cat #D4902 Cyclohexane Sigma-Aldrich Cat #C698 Dimethyl sulfoxide Sigma-Aldrich Cat #D2650 D-Luciferin, Potassium Salt GOLDBIO Cat # 010637LUCK X-Gluc GOLDBIO Cat #G1281C1 DAPI BBI Cat #E607303 Protease Inhibitor Cocktails Roche Cat # 11873580001 PMSF Roche Cat # 10837091001 Nonidet-P40 Roche Cat # 14883100 Poly dI dC Sigma-Aldrich Cat #P0913 PhytagelTM Sigma-Aldrich Cat #P8169-500G Protein A beads New England Biolabs Cat #S1425 Cellulase R10 Yakult Japan Cat # 180612-02 Maecrozyme R10 Yakult Japan Cat # 171208-02 T5 Exonuclease Epicentre Cat #T5E4111K DNA Polymerase New England Biolabs Cat #M0535S Taq DNA Ligase New England Biolabs Cat #M0208L GST-OsTOC1 This paper N/A Critical commercial assays ReverTre Ace Qpcr RT Master Mix TOYOBO Cat # FSQ-301 23SYB Mix Transgen Cat # AQ601-01 Dual-Luciferase Reporter Assay System Promega Cat #E1500 QubitTM dsDNA HS Assay Invitrogen Cat #Q32851 NEBNext UltraTM II DNA Library Prep Kit for Illumina New England Biolabs Cat #E7645S NEBNext Multiplex Oligos for Illumina New England Biolabs Cat #E7335 KOD FX TOYOBO Cat # KFX-101 Kimura B nutrient salts Solution Coolaber Cat # NS1050-2000L Chemiluminescent Detection Module *Component of DAFA Kits Thermo Fisher Scientific Cat # 89880 (Continued on next page) 14 Cell Reports 42, 112765, July 25, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Raw RNA-seq data https://ngdc.cncb.ac.cn PRJCA016700 Raw ChIP-seq data https://ngdc.cncb.ac.cn PRJCA016659 Experimental models: Organisms/strains Oryza sativa: OsTOC1-ox This paper N/A Oryza sativa: OsTOC1-RNAi This paper N/A Oryza sativa: Ostoc1 This paper N/A Oryza sativa: OsTOC1-GFP-GR This paper N/A Oryza sativa: OsTMG This paper N/A Oryza sativa: pOsTOC1:GUS This paper N/A Nicotiana benthamiana This paper N/A Oligonucleotides See Table S2 This paper N/A Recombinant DNA pUbi:OsTOC1 GFP/pCABR1:LUC This paper N/A CRISPR/Cas9 OsTOC1/pCABR1:LUC This paper N/A pUbi:OsTOC1-GFP-GR This paper N/A pOsTOC1:GUS This paper N/A pOsTOC1:OsTOC1-GFP This paper N/A pUbi:OsTOC1-antiOsTOC1 This paper N/A pGEX 4T-1 GST-OsTOC1 This paper N/A pGreen0800 62-SK-OsTOC1 This paper N/A 35s:GAL4BD-VP16-OsTOC1 This paper N/A 35s:GAL4BD-VP16-AtTOC1 This paper N/A pGreen0800 pOsTGAL3a:LUC This paper N/A pGreen0800 pOsTGAL3b:LUC This paper N/A pGreen0800 pmOsTGAL3a:LUC(mOsTBM) This paper N/A pGreen0800 pOsIAA12:LUC This paper N/A pGreen0800 pOsIAA27:LUC This paper N/A pGreen0800 pOsGI:LUC This paper N/A pGreen0800 pOsLHY:LUC This paper N/A Software and algorithms ImageJ Schneider et al.7 https://imagej.nih.gov/ij/ Graphpad grism GraphPad Software www.graphpad.com MEGA7.0 Molecular Evolutionary Genetic Analysis https://www.megasoftware.net/ IGV 2.12.0 Integrative Genomics Viewer https://software.broadinstitute.org/software/igv Other Confocal microscope ZEISS LSM 7810 Plant growth chamber Percival Scientific E 41L2 Microplate luminometer Berthold Tristar2 S LB 942 ..

    Recombinant:

    Article Title: OsTOC1 plays dual roles in the regulation of plant circadian clock by functioning as a direct transcription activator or repressor.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies GFP Polyclonal Antibody Invitrogen Cat # A-11122; RRID: AB_221569 Actin (26F7) mAb for PLANTs Abmart Cat #M20009; RRID: AB_2936239 Anti-Histone H3 antibody Abcam Cat # ab1791; RRID: AB_302613 Goat anti-Rabbit IgG (H + L) Secondary Antibody, HRP Invitrogen Cat # 31460; RRID: AB_228341 Anti-mouse IgG, HRP-linked Antibody Cell Signaling Technology Cat # 7076; RRID; AB_330924 Bacterial and virus strains Escherichia coli strain DH10B N/A N/A Escherichia coli strain BL21(AI) Weidi Cat # EC1004 Escherichia coli strain NS3529 N/A N/A Agrobacterium tumefaciens GV3101(pSoup-p19) Weidi Cat # AC1003 Xanthomonas oryzae pv. oryzae N/A N/A Agrobacterium tumefaciens EHA105 Weidi Cat # AC1010 Chemicals, peptides, and recombinant proteins TRIzolTM reagent Invitrogen Cat # 15596026 Dexamethasone Sigma-Aldrich Cat #D4902 Cyclohexane Sigma-Aldrich Cat #C698 Dimethyl sulfoxide Sigma-Aldrich Cat #D2650 D-Luciferin, Potassium Salt GOLDBIO Cat # 010637LUCK X-Gluc GOLDBIO Cat #G1281C1 DAPI BBI Cat #E607303 Protease Inhibitor Cocktails Roche Cat # 11873580001 PMSF Roche Cat # 10837091001 Nonidet-P40 Roche Cat # 14883100 Poly dI dC Sigma-Aldrich Cat #P0913 PhytagelTM Sigma-Aldrich Cat #P8169-500G Protein A beads New England Biolabs Cat #S1425 Cellulase R10 Yakult Japan Cat # 180612-02 Maecrozyme R10 Yakult Japan Cat # 171208-02 T5 Exonuclease Epicentre Cat #T5E4111K DNA Polymerase New England Biolabs Cat #M0535S Taq DNA Ligase New England Biolabs Cat #M0208L GST-OsTOC1 This paper N/A Critical commercial assays ReverTre Ace Qpcr RT Master Mix TOYOBO Cat # FSQ-301 23SYB Mix Transgen Cat # AQ601-01 Dual-Luciferase Reporter Assay System Promega Cat #E1500 QubitTM dsDNA HS Assay Invitrogen Cat #Q32851 NEBNext UltraTM II DNA Library Prep Kit for Illumina New England Biolabs Cat #E7645S NEBNext Multiplex Oligos for Illumina New England Biolabs Cat #E7335 KOD FX TOYOBO Cat # KFX-101 Kimura B nutrient salts Solution Coolaber Cat # NS1050-2000L Chemiluminescent Detection Module *Component of DAFA Kits Thermo Fisher Scientific Cat # 89880 (Continued on next page) 14 Cell Reports 42, 112765, July 25, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Raw RNA-seq data https://ngdc.cncb.ac.cn PRJCA016700 Raw ChIP-seq data https://ngdc.cncb.ac.cn PRJCA016659 Experimental models: Organisms/strains Oryza sativa: OsTOC1-ox This paper N/A Oryza sativa: OsTOC1-RNAi This paper N/A Oryza sativa: Ostoc1 This paper N/A Oryza sativa: OsTOC1-GFP-GR This paper N/A Oryza sativa: OsTMG This paper N/A Oryza sativa: pOsTOC1:GUS This paper N/A Nicotiana benthamiana This paper N/A Oligonucleotides See Table S2 This paper N/A Recombinant DNA pUbi:OsTOC1 GFP/pCABR1:LUC This paper N/A CRISPR/Cas9 OsTOC1/pCABR1:LUC This paper N/A pUbi:OsTOC1-GFP-GR This paper N/A pOsTOC1:GUS This paper N/A pOsTOC1:OsTOC1-GFP This paper N/A pUbi:OsTOC1-antiOsTOC1 This paper N/A pGEX 4T-1 GST-OsTOC1 This paper N/A pGreen0800 62-SK-OsTOC1 This paper N/A 35s:GAL4BD-VP16-OsTOC1 This paper N/A 35s:GAL4BD-VP16-AtTOC1 This paper N/A pGreen0800 pOsTGAL3a:LUC This paper N/A pGreen0800 pOsTGAL3b:LUC This paper N/A pGreen0800 pmOsTGAL3a:LUC(mOsTBM) This paper N/A pGreen0800 pOsIAA12:LUC This paper N/A pGreen0800 pOsIAA27:LUC This paper N/A pGreen0800 pOsGI:LUC This paper N/A pGreen0800 pOsLHY:LUC This paper N/A Software and algorithms ImageJ Schneider et al.7 https://imagej.nih.gov/ij/ Graphpad grism GraphPad Software www.graphpad.com MEGA7.0 Molecular Evolutionary Genetic Analysis https://www.megasoftware.net/ IGV 2.12.0 Integrative Genomics Viewer https://software.broadinstitute.org/software/igv Other Confocal microscope ZEISS LSM 7810 Plant growth chamber Percival Scientific E 41L2 Microplate luminometer Berthold Tristar2 S LB 942 ..

    CRISPR:

    Article Title: OsTOC1 plays dual roles in the regulation of plant circadian clock by functioning as a direct transcription activator or repressor.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies GFP Polyclonal Antibody Invitrogen Cat # A-11122; RRID: AB_221569 Actin (26F7) mAb for PLANTs Abmart Cat #M20009; RRID: AB_2936239 Anti-Histone H3 antibody Abcam Cat # ab1791; RRID: AB_302613 Goat anti-Rabbit IgG (H + L) Secondary Antibody, HRP Invitrogen Cat # 31460; RRID: AB_228341 Anti-mouse IgG, HRP-linked Antibody Cell Signaling Technology Cat # 7076; RRID; AB_330924 Bacterial and virus strains Escherichia coli strain DH10B N/A N/A Escherichia coli strain BL21(AI) Weidi Cat # EC1004 Escherichia coli strain NS3529 N/A N/A Agrobacterium tumefaciens GV3101(pSoup-p19) Weidi Cat # AC1003 Xanthomonas oryzae pv. oryzae N/A N/A Agrobacterium tumefaciens EHA105 Weidi Cat # AC1010 Chemicals, peptides, and recombinant proteins TRIzolTM reagent Invitrogen Cat # 15596026 Dexamethasone Sigma-Aldrich Cat #D4902 Cyclohexane Sigma-Aldrich Cat #C698 Dimethyl sulfoxide Sigma-Aldrich Cat #D2650 D-Luciferin, Potassium Salt GOLDBIO Cat # 010637LUCK X-Gluc GOLDBIO Cat #G1281C1 DAPI BBI Cat #E607303 Protease Inhibitor Cocktails Roche Cat # 11873580001 PMSF Roche Cat # 10837091001 Nonidet-P40 Roche Cat # 14883100 Poly dI dC Sigma-Aldrich Cat #P0913 PhytagelTM Sigma-Aldrich Cat #P8169-500G Protein A beads New England Biolabs Cat #S1425 Cellulase R10 Yakult Japan Cat # 180612-02 Maecrozyme R10 Yakult Japan Cat # 171208-02 T5 Exonuclease Epicentre Cat #T5E4111K DNA Polymerase New England Biolabs Cat #M0535S Taq DNA Ligase New England Biolabs Cat #M0208L GST-OsTOC1 This paper N/A Critical commercial assays ReverTre Ace Qpcr RT Master Mix TOYOBO Cat # FSQ-301 23SYB Mix Transgen Cat # AQ601-01 Dual-Luciferase Reporter Assay System Promega Cat #E1500 QubitTM dsDNA HS Assay Invitrogen Cat #Q32851 NEBNext UltraTM II DNA Library Prep Kit for Illumina New England Biolabs Cat #E7645S NEBNext Multiplex Oligos for Illumina New England Biolabs Cat #E7335 KOD FX TOYOBO Cat # KFX-101 Kimura B nutrient salts Solution Coolaber Cat # NS1050-2000L Chemiluminescent Detection Module *Component of DAFA Kits Thermo Fisher Scientific Cat # 89880 (Continued on next page) 14 Cell Reports 42, 112765, July 25, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Raw RNA-seq data https://ngdc.cncb.ac.cn PRJCA016700 Raw ChIP-seq data https://ngdc.cncb.ac.cn PRJCA016659 Experimental models: Organisms/strains Oryza sativa: OsTOC1-ox This paper N/A Oryza sativa: OsTOC1-RNAi This paper N/A Oryza sativa: Ostoc1 This paper N/A Oryza sativa: OsTOC1-GFP-GR This paper N/A Oryza sativa: OsTMG This paper N/A Oryza sativa: pOsTOC1:GUS This paper N/A Nicotiana benthamiana This paper N/A Oligonucleotides See Table S2 This paper N/A Recombinant DNA pUbi:OsTOC1 GFP/pCABR1:LUC This paper N/A CRISPR/Cas9 OsTOC1/pCABR1:LUC This paper N/A pUbi:OsTOC1-GFP-GR This paper N/A pOsTOC1:GUS This paper N/A pOsTOC1:OsTOC1-GFP This paper N/A pUbi:OsTOC1-antiOsTOC1 This paper N/A pGEX 4T-1 GST-OsTOC1 This paper N/A pGreen0800 62-SK-OsTOC1 This paper N/A 35s:GAL4BD-VP16-OsTOC1 This paper N/A 35s:GAL4BD-VP16-AtTOC1 This paper N/A pGreen0800 pOsTGAL3a:LUC This paper N/A pGreen0800 pOsTGAL3b:LUC This paper N/A pGreen0800 pmOsTGAL3a:LUC(mOsTBM) This paper N/A pGreen0800 pOsIAA12:LUC This paper N/A pGreen0800 pOsIAA27:LUC This paper N/A pGreen0800 pOsGI:LUC This paper N/A pGreen0800 pOsLHY:LUC This paper N/A Software and algorithms ImageJ Schneider et al.7 https://imagej.nih.gov/ij/ Graphpad grism GraphPad Software www.graphpad.com MEGA7.0 Molecular Evolutionary Genetic Analysis https://www.megasoftware.net/ IGV 2.12.0 Integrative Genomics Viewer https://software.broadinstitute.org/software/igv Other Confocal microscope ZEISS LSM 7810 Plant growth chamber Percival Scientific E 41L2 Microplate luminometer Berthold Tristar2 S LB 942 ..

    Software:

    Article Title: OsTOC1 plays dual roles in the regulation of plant circadian clock by functioning as a direct transcription activator or repressor.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies GFP Polyclonal Antibody Invitrogen Cat # A-11122; RRID: AB_221569 Actin (26F7) mAb for PLANTs Abmart Cat #M20009; RRID: AB_2936239 Anti-Histone H3 antibody Abcam Cat # ab1791; RRID: AB_302613 Goat anti-Rabbit IgG (H + L) Secondary Antibody, HRP Invitrogen Cat # 31460; RRID: AB_228341 Anti-mouse IgG, HRP-linked Antibody Cell Signaling Technology Cat # 7076; RRID; AB_330924 Bacterial and virus strains Escherichia coli strain DH10B N/A N/A Escherichia coli strain BL21(AI) Weidi Cat # EC1004 Escherichia coli strain NS3529 N/A N/A Agrobacterium tumefaciens GV3101(pSoup-p19) Weidi Cat # AC1003 Xanthomonas oryzae pv. oryzae N/A N/A Agrobacterium tumefaciens EHA105 Weidi Cat # AC1010 Chemicals, peptides, and recombinant proteins TRIzolTM reagent Invitrogen Cat # 15596026 Dexamethasone Sigma-Aldrich Cat #D4902 Cyclohexane Sigma-Aldrich Cat #C698 Dimethyl sulfoxide Sigma-Aldrich Cat #D2650 D-Luciferin, Potassium Salt GOLDBIO Cat # 010637LUCK X-Gluc GOLDBIO Cat #G1281C1 DAPI BBI Cat #E607303 Protease Inhibitor Cocktails Roche Cat # 11873580001 PMSF Roche Cat # 10837091001 Nonidet-P40 Roche Cat # 14883100 Poly dI dC Sigma-Aldrich Cat #P0913 PhytagelTM Sigma-Aldrich Cat #P8169-500G Protein A beads New England Biolabs Cat #S1425 Cellulase R10 Yakult Japan Cat # 180612-02 Maecrozyme R10 Yakult Japan Cat # 171208-02 T5 Exonuclease Epicentre Cat #T5E4111K DNA Polymerase New England Biolabs Cat #M0535S Taq DNA Ligase New England Biolabs Cat #M0208L GST-OsTOC1 This paper N/A Critical commercial assays ReverTre Ace Qpcr RT Master Mix TOYOBO Cat # FSQ-301 23SYB Mix Transgen Cat # AQ601-01 Dual-Luciferase Reporter Assay System Promega Cat #E1500 QubitTM dsDNA HS Assay Invitrogen Cat #Q32851 NEBNext UltraTM II DNA Library Prep Kit for Illumina New England Biolabs Cat #E7645S NEBNext Multiplex Oligos for Illumina New England Biolabs Cat #E7335 KOD FX TOYOBO Cat # KFX-101 Kimura B nutrient salts Solution Coolaber Cat # NS1050-2000L Chemiluminescent Detection Module *Component of DAFA Kits Thermo Fisher Scientific Cat # 89880 (Continued on next page) 14 Cell Reports 42, 112765, July 25, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Raw RNA-seq data https://ngdc.cncb.ac.cn PRJCA016700 Raw ChIP-seq data https://ngdc.cncb.ac.cn PRJCA016659 Experimental models: Organisms/strains Oryza sativa: OsTOC1-ox This paper N/A Oryza sativa: OsTOC1-RNAi This paper N/A Oryza sativa: Ostoc1 This paper N/A Oryza sativa: OsTOC1-GFP-GR This paper N/A Oryza sativa: OsTMG This paper N/A Oryza sativa: pOsTOC1:GUS This paper N/A Nicotiana benthamiana This paper N/A Oligonucleotides See Table S2 This paper N/A Recombinant DNA pUbi:OsTOC1 GFP/pCABR1:LUC This paper N/A CRISPR/Cas9 OsTOC1/pCABR1:LUC This paper N/A pUbi:OsTOC1-GFP-GR This paper N/A pOsTOC1:GUS This paper N/A pOsTOC1:OsTOC1-GFP This paper N/A pUbi:OsTOC1-antiOsTOC1 This paper N/A pGEX 4T-1 GST-OsTOC1 This paper N/A pGreen0800 62-SK-OsTOC1 This paper N/A 35s:GAL4BD-VP16-OsTOC1 This paper N/A 35s:GAL4BD-VP16-AtTOC1 This paper N/A pGreen0800 pOsTGAL3a:LUC This paper N/A pGreen0800 pOsTGAL3b:LUC This paper N/A pGreen0800 pmOsTGAL3a:LUC(mOsTBM) This paper N/A pGreen0800 pOsIAA12:LUC This paper N/A pGreen0800 pOsIAA27:LUC This paper N/A pGreen0800 pOsGI:LUC This paper N/A pGreen0800 pOsLHY:LUC This paper N/A Software and algorithms ImageJ Schneider et al.7 https://imagej.nih.gov/ij/ Graphpad grism GraphPad Software www.graphpad.com MEGA7.0 Molecular Evolutionary Genetic Analysis https://www.megasoftware.net/ IGV 2.12.0 Integrative Genomics Viewer https://software.broadinstitute.org/software/igv Other Confocal microscope ZEISS LSM 7810 Plant growth chamber Percival Scientific E 41L2 Microplate luminometer Berthold Tristar2 S LB 942 ..

    Microscopy:

    Article Title: OsTOC1 plays dual roles in the regulation of plant circadian clock by functioning as a direct transcription activator or repressor.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies GFP Polyclonal Antibody Invitrogen Cat # A-11122; RRID: AB_221569 Actin (26F7) mAb for PLANTs Abmart Cat #M20009; RRID: AB_2936239 Anti-Histone H3 antibody Abcam Cat # ab1791; RRID: AB_302613 Goat anti-Rabbit IgG (H + L) Secondary Antibody, HRP Invitrogen Cat # 31460; RRID: AB_228341 Anti-mouse IgG, HRP-linked Antibody Cell Signaling Technology Cat # 7076; RRID; AB_330924 Bacterial and virus strains Escherichia coli strain DH10B N/A N/A Escherichia coli strain BL21(AI) Weidi Cat # EC1004 Escherichia coli strain NS3529 N/A N/A Agrobacterium tumefaciens GV3101(pSoup-p19) Weidi Cat # AC1003 Xanthomonas oryzae pv. oryzae N/A N/A Agrobacterium tumefaciens EHA105 Weidi Cat # AC1010 Chemicals, peptides, and recombinant proteins TRIzolTM reagent Invitrogen Cat # 15596026 Dexamethasone Sigma-Aldrich Cat #D4902 Cyclohexane Sigma-Aldrich Cat #C698 Dimethyl sulfoxide Sigma-Aldrich Cat #D2650 D-Luciferin, Potassium Salt GOLDBIO Cat # 010637LUCK X-Gluc GOLDBIO Cat #G1281C1 DAPI BBI Cat #E607303 Protease Inhibitor Cocktails Roche Cat # 11873580001 PMSF Roche Cat # 10837091001 Nonidet-P40 Roche Cat # 14883100 Poly dI dC Sigma-Aldrich Cat #P0913 PhytagelTM Sigma-Aldrich Cat #P8169-500G Protein A beads New England Biolabs Cat #S1425 Cellulase R10 Yakult Japan Cat # 180612-02 Maecrozyme R10 Yakult Japan Cat # 171208-02 T5 Exonuclease Epicentre Cat #T5E4111K DNA Polymerase New England Biolabs Cat #M0535S Taq DNA Ligase New England Biolabs Cat #M0208L GST-OsTOC1 This paper N/A Critical commercial assays ReverTre Ace Qpcr RT Master Mix TOYOBO Cat # FSQ-301 23SYB Mix Transgen Cat # AQ601-01 Dual-Luciferase Reporter Assay System Promega Cat #E1500 QubitTM dsDNA HS Assay Invitrogen Cat #Q32851 NEBNext UltraTM II DNA Library Prep Kit for Illumina New England Biolabs Cat #E7645S NEBNext Multiplex Oligos for Illumina New England Biolabs Cat #E7335 KOD FX TOYOBO Cat # KFX-101 Kimura B nutrient salts Solution Coolaber Cat # NS1050-2000L Chemiluminescent Detection Module *Component of DAFA Kits Thermo Fisher Scientific Cat # 89880 (Continued on next page) 14 Cell Reports 42, 112765, July 25, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Raw RNA-seq data https://ngdc.cncb.ac.cn PRJCA016700 Raw ChIP-seq data https://ngdc.cncb.ac.cn PRJCA016659 Experimental models: Organisms/strains Oryza sativa: OsTOC1-ox This paper N/A Oryza sativa: OsTOC1-RNAi This paper N/A Oryza sativa: Ostoc1 This paper N/A Oryza sativa: OsTOC1-GFP-GR This paper N/A Oryza sativa: OsTMG This paper N/A Oryza sativa: pOsTOC1:GUS This paper N/A Nicotiana benthamiana This paper N/A Oligonucleotides See Table S2 This paper N/A Recombinant DNA pUbi:OsTOC1 GFP/pCABR1:LUC This paper N/A CRISPR/Cas9 OsTOC1/pCABR1:LUC This paper N/A pUbi:OsTOC1-GFP-GR This paper N/A pOsTOC1:GUS This paper N/A pOsTOC1:OsTOC1-GFP This paper N/A pUbi:OsTOC1-antiOsTOC1 This paper N/A pGEX 4T-1 GST-OsTOC1 This paper N/A pGreen0800 62-SK-OsTOC1 This paper N/A 35s:GAL4BD-VP16-OsTOC1 This paper N/A 35s:GAL4BD-VP16-AtTOC1 This paper N/A pGreen0800 pOsTGAL3a:LUC This paper N/A pGreen0800 pOsTGAL3b:LUC This paper N/A pGreen0800 pmOsTGAL3a:LUC(mOsTBM) This paper N/A pGreen0800 pOsIAA12:LUC This paper N/A pGreen0800 pOsIAA27:LUC This paper N/A pGreen0800 pOsGI:LUC This paper N/A pGreen0800 pOsLHY:LUC This paper N/A Software and algorithms ImageJ Schneider et al.7 https://imagej.nih.gov/ij/ Graphpad grism GraphPad Software www.graphpad.com MEGA7.0 Molecular Evolutionary Genetic Analysis https://www.megasoftware.net/ IGV 2.12.0 Integrative Genomics Viewer https://software.broadinstitute.org/software/igv Other Confocal microscope ZEISS LSM 7810 Plant growth chamber Percival Scientific E 41L2 Microplate luminometer Berthold Tristar2 S LB 942 ..



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