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Syngene genetools imagequant software
Genetools Imagequant Software, supplied by Syngene, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/md+imagequant+software/genetools+software/pm15564324-98-7-10
Average 86 stars, based on 1 article reviews
genetools imagequant software - by Bioz Stars, 2026-10
86/100 stars

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Related Articles

Polymerase Chain Reaction:

Article Title: Phosphorus availability drives rhizosphere metabolite-microbial community interactions to modulate cucumber susceptibility to Fusarium wilt.
Article Snippet: PCR amplification was performed using a Bio-Rad S1000 (Bio-Rad Laboratory) with the following cycles: 95 °C for 5 min, then 30 cycles of 94 °C for 30 s, 52 °C for 30 s, and 72 °C for 30 s with a final extension at 72 °C for 10 min. Products were separated on a 1% agarose gel and those with clear bands between 290 and 310 bp were combined for sequencing. .. PCR products were mixed in ratios of equal density according to GeneTools analysis software (version 4.03.05.0, SynGene), and the mixtures were purified with an E. Z. N. A Gel Extraction Kit (Omega Bio-tek, Norcross, GA, USA). .. Sequencing libraries were generated using the NEBNext® UltraTM DNA Library Prep Kit for Illumina® (New England Bio-labs, Ipswich, MA, USA) following the manufacturer recommendations.

Article Title: Convergent heavy metal profiles shape divergent bacterial community patterns in subtropical water-sediment system.
Article Snippet: • Subtropical water and sediments carry similar metals but different bacteria.. • Reduced dilution brought dry season higher heavy metal contamination risks.. • Random processes affected the bacterial community assembly in sediments

Article Title: Analysis of endophytic bacteria diversity between two species of genus Polygala with similar medicinal value.
Article Snippet: .. AR TIC LE IN PR ES S ARTICLE IN PRESS We compared the concentrations of PCR products using GeneTools Analysis Software (Version 4.03.05.0, SynGene), and required volumes were calculated for each sample based on the principle of equal mass, after which PCR products were mixed. ..

Article Title: Decreasing light availability inhibits the nitrogen fixation of biological soil crusts in dryland ecosystems
Article Snippet: Nitrogen (N) fixation by biological soil crusts (BSCs) is crucial for dryland ecosystems but is energy-intensive and relies on photosynthesis.. Despite the potential impact of changing light availability due to shrub encroachment in drylands, direct field evidence linking light to BSC N fixation remains limited.. We conducted a six-month field experiment on China's Loess Plateau to examine how shading (2% to 100% irradiance) affects N fixation in mossand cyanobacteria-dominated BSCs, hypothesizing that decreased light availability would significantly impact their N fixation capacity.

Article Title: The Ganoderma lucidum and Rosa roxburghii Tratt Formulation Prevents Depressive-like Behaviors in Mice by Modulating Tryptophan Metabolism via the Gut-Brain Axis and Upregulating the BDNF/TrkB/PI3K/AKT Pathway.
Article Snippet: The thermocycling conditions were: 94 ◦C for 5 min, 30 cycles of 94 ◦C for 30 s, 52 ◦C for 30 s, 72 ◦C for 30 s, and a final extension at 72 ◦C for 10 min. We carried out 1.5% agarose gel electrophoresis at 120 V for 30 min, using a DL2000 DNA marker (Takara, Dalian, China) to check the fragment length and concentration of the PCR product. .. Concentrations of the PCR products were compared and subsequently mixed, following the data requirements, utilizing GeneTools Analysis Software version 4.03.05.0 (SynGene, Cambridge, UK). .. The library was prepared using the standard protocol associated with the ALFA-SEQ DNA library prep kit, and the sequencing of the amplicon library was conducted on the Illumina platform utilizing PE250 technology.

Software:

Article Title: Phosphorus availability drives rhizosphere metabolite-microbial community interactions to modulate cucumber susceptibility to Fusarium wilt.
Article Snippet: PCR amplification was performed using a Bio-Rad S1000 (Bio-Rad Laboratory) with the following cycles: 95 °C for 5 min, then 30 cycles of 94 °C for 30 s, 52 °C for 30 s, and 72 °C for 30 s with a final extension at 72 °C for 10 min. Products were separated on a 1% agarose gel and those with clear bands between 290 and 310 bp were combined for sequencing. .. PCR products were mixed in ratios of equal density according to GeneTools analysis software (version 4.03.05.0, SynGene), and the mixtures were purified with an E. Z. N. A Gel Extraction Kit (Omega Bio-tek, Norcross, GA, USA). .. Sequencing libraries were generated using the NEBNext® UltraTM DNA Library Prep Kit for Illumina® (New England Bio-labs, Ipswich, MA, USA) following the manufacturer recommendations.

Article Title: Convergent heavy metal profiles shape divergent bacterial community patterns in subtropical water-sediment system.
Article Snippet: • Subtropical water and sediments carry similar metals but different bacteria.. • Reduced dilution brought dry season higher heavy metal contamination risks.. • Random processes affected the bacterial community assembly in sediments

Article Title: Analysis of endophytic bacteria diversity between two species of genus Polygala with similar medicinal value.
Article Snippet: .. AR TIC LE IN PR ES S ARTICLE IN PRESS We compared the concentrations of PCR products using GeneTools Analysis Software (Version 4.03.05.0, SynGene), and required volumes were calculated for each sample based on the principle of equal mass, after which PCR products were mixed. ..

Article Title: Disrupting the Feed-Forward Cycle of RyR1 Ca 2+ Leak and Oxidative Stress Mitigates Doxorubicin-Induced Skeletal Myopathy.
Article Snippet: Department of Physiology and Biophysics, Rush University Medical Center, Chicago, 60612 9 USA.. 10 Group of Neuromuscular Diseases, Biogipuzkoa Health Research Institute and CIBERNED, 11 ISCIII (CIBER, Carlos III Institute, Spanish Ministry of Sciences and Innovation), 20014 Donostia12 San Sebastian, Spain.. 13 Group of Neurosciences, Departments of Pediatrics and Neuroscience, Faculty of Medicine and 14 Nursing, University of Basque Country (UPV/EHU), 20014 Donostia-San Sebastian, Spain.

Article Title: KEAP1-mediated mitochondrial remodeling on stiffness-graded collagen substrates drives hepatocellular carcinoma adaptation.
Article Snippet: Mechanical and structural cues of the extracellular matrix (ECM) regulate tumor cell metabolism and drug response, yet the molecular mediators that link these biophysical signals are unclear.. We present an elastically supported surface platform bioengineered with varying stiffness and collagen fiber anisotropy to replicate the fibrotic and mechanically inhomogeneous liver cancer microenvironment.. Using this system, we identify Kelchlike ECH-associated protein 1 (KEAP1) as a mechanoresponsive mediator linking ECM stiffness to mitochondrial dynamics, redox adaptation, and chemoresistance in hepatocellular carcinoma (HCC).

Article Title: The impact of Triticum aestivum L. cultivation management on genomic polymorphism of Harpalus rufipes and Silpha obscura assessed by RAPD markers.
Article Snippet: Amplified products were separated by electrophoresis on a 1.5% agarose gel, stained with GelRedTM Nucleic Acid Stain (Biotium), and visualized using a G-Box imaging system (SYNGENE). .. The banding patterns were interpreted using GeneTools software (SYNGENE), which enables automatic detection of distinct amplicons based on signal intensity. .. Presence or absence of individual bands was encoded into a binary format to generate matrices, which were subsequently analyzed in the R environment (R Core Team 2020), using dplyr, stats, pheatmap, and RColorBrewer packages (RStudio Team 2020).

Article Title: Decreasing light availability inhibits the nitrogen fixation of biological soil crusts in dryland ecosystems
Article Snippet: Nitrogen (N) fixation by biological soil crusts (BSCs) is crucial for dryland ecosystems but is energy-intensive and relies on photosynthesis.. Despite the potential impact of changing light availability due to shrub encroachment in drylands, direct field evidence linking light to BSC N fixation remains limited.. We conducted a six-month field experiment on China's Loess Plateau to examine how shading (2% to 100% irradiance) affects N fixation in mossand cyanobacteria-dominated BSCs, hypothesizing that decreased light availability would significantly impact their N fixation capacity.

Article Title: The Ganoderma lucidum and Rosa roxburghii Tratt Formulation Prevents Depressive-like Behaviors in Mice by Modulating Tryptophan Metabolism via the Gut-Brain Axis and Upregulating the BDNF/TrkB/PI3K/AKT Pathway.
Article Snippet: The thermocycling conditions were: 94 ◦C for 5 min, 30 cycles of 94 ◦C for 30 s, 52 ◦C for 30 s, 72 ◦C for 30 s, and a final extension at 72 ◦C for 10 min. We carried out 1.5% agarose gel electrophoresis at 120 V for 30 min, using a DL2000 DNA marker (Takara, Dalian, China) to check the fragment length and concentration of the PCR product. .. Concentrations of the PCR products were compared and subsequently mixed, following the data requirements, utilizing GeneTools Analysis Software version 4.03.05.0 (SynGene, Cambridge, UK). .. The library was prepared using the standard protocol associated with the ALFA-SEQ DNA library prep kit, and the sequencing of the amplicon library was conducted on the Illumina platform utilizing PE250 technology.

Purification:

Article Title: Phosphorus availability drives rhizosphere metabolite-microbial community interactions to modulate cucumber susceptibility to Fusarium wilt.
Article Snippet: PCR amplification was performed using a Bio-Rad S1000 (Bio-Rad Laboratory) with the following cycles: 95 °C for 5 min, then 30 cycles of 94 °C for 30 s, 52 °C for 30 s, and 72 °C for 30 s with a final extension at 72 °C for 10 min. Products were separated on a 1% agarose gel and those with clear bands between 290 and 310 bp were combined for sequencing. .. PCR products were mixed in ratios of equal density according to GeneTools analysis software (version 4.03.05.0, SynGene), and the mixtures were purified with an E. Z. N. A Gel Extraction Kit (Omega Bio-tek, Norcross, GA, USA). .. Sequencing libraries were generated using the NEBNext® UltraTM DNA Library Prep Kit for Illumina® (New England Bio-labs, Ipswich, MA, USA) following the manufacturer recommendations.

Gel Extraction:

Article Title: Phosphorus availability drives rhizosphere metabolite-microbial community interactions to modulate cucumber susceptibility to Fusarium wilt.
Article Snippet: PCR amplification was performed using a Bio-Rad S1000 (Bio-Rad Laboratory) with the following cycles: 95 °C for 5 min, then 30 cycles of 94 °C for 30 s, 52 °C for 30 s, and 72 °C for 30 s with a final extension at 72 °C for 10 min. Products were separated on a 1% agarose gel and those with clear bands between 290 and 310 bp were combined for sequencing. .. PCR products were mixed in ratios of equal density according to GeneTools analysis software (version 4.03.05.0, SynGene), and the mixtures were purified with an E. Z. N. A Gel Extraction Kit (Omega Bio-tek, Norcross, GA, USA). .. Sequencing libraries were generated using the NEBNext® UltraTM DNA Library Prep Kit for Illumina® (New England Bio-labs, Ipswich, MA, USA) following the manufacturer recommendations.

Amplification:

Article Title: Convergent heavy metal profiles shape divergent bacterial community patterns in subtropical water-sediment system.
Article Snippet: • Subtropical water and sediments carry similar metals but different bacteria.. • Reduced dilution brought dry season higher heavy metal contamination risks.. • Random processes affected the bacterial community assembly in sediments



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