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mcf  (ATCC)


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    ATCC mcf
    Mcf, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 35361 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mcf7/MCF7/pmc13101642-75-0-9
    Average 99 stars, based on 35361 article reviews
    mcf - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    Multiple Displacement Amplification:

    Article Title: IL-1β/IL-6 inflammatory crosstalk drives DNA methylation and histone remodeling associated with cytokine induced senescence in luminal breast cancer
    Article Snippet: .. MCF-7 (CVCL_0031), MDA-MB-231 (CVCL_0062) and MCF-10 A (CVCL_0598) cell lines were procured from ATCC and cultured according to supplier’s protocol. ..

    Article Title: LINC01537 Regulates Malignant Phenotypes and Predicts Prognosis in Breast Cancer, and Its Bioinformatics Analysis
    Article Snippet: .. The normal human mammary epithelial cell line MCF10A and breast cancer cell lines MDA‐MB‐453, MDA‐MB‐231, BT549, MCF‐7, and SK‐BR‐3 were obtained from the American Type Culture Collection (ATCC). .. MCF‐7 and MDA‐MB‐231 cells were grown in RPMI‐1640, while MCF10A, BT549, and MDA‐MB‐453 were maintained in DMEM (Invitrogen).

    Cell Culture:

    Article Title: IL-1β/IL-6 inflammatory crosstalk drives DNA methylation and histone remodeling associated with cytokine induced senescence in luminal breast cancer
    Article Snippet: .. MCF-7 (CVCL_0031), MDA-MB-231 (CVCL_0062) and MCF-10 A (CVCL_0598) cell lines were procured from ATCC and cultured according to supplier’s protocol. ..

    Article Title: Design and synthesis of hydrolyzable coumarin–naphthalimide dual probes for NIR fluorescence detection and selective photodynamic activity in cancer cells
    Article Snippet: .. Human embryonic kidney cells HEK-293 (ATCC CRL-1573), used as a healthy model, and MCF-7 adenocarcinoma breast cancer cells (ATCC HTB-22) were cultured in Dulbecco's Modified Eagle's Medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 100 U mL −1 penicillin, and 100 μg mL −1 streptomycin at 37 °C, 5% CO 2 , and 100% humidity. ..

    Modification:

    Article Title: Design and synthesis of hydrolyzable coumarin–naphthalimide dual probes for NIR fluorescence detection and selective photodynamic activity in cancer cells
    Article Snippet: .. Human embryonic kidney cells HEK-293 (ATCC CRL-1573), used as a healthy model, and MCF-7 adenocarcinoma breast cancer cells (ATCC HTB-22) were cultured in Dulbecco's Modified Eagle's Medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 100 U mL −1 penicillin, and 100 μg mL −1 streptomycin at 37 °C, 5% CO 2 , and 100% humidity. ..

    Flow Cytometry:

    Article Title: Polymerase iota coordinates adaptation to replication stress in BRCA2-deficient mammary epithelial cells.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-FANCD2 (Clone EPR2302) Abcam Cat# ab108928, RRID: AB_10862535 Mouse monoclonal anti-phospho-Histone H3 Ser10 (Clone 6G3) Cell Signaling Technology Cat# 9706, RRID: AB_331748 Mouse monoclonal anti-Cyclin A2 (Clone 1C6C11) Proteintech Cat# 66391-1-Ig, RRID: AB_2881767 Rabbit polyclonal anti-53BP1 Cell Signaling Technology Cat# 4937, RRID: AB_10694558 Rat monoclonal anti-RPA32/RPA2 (Clone 4E4) Cell Signaling Technology Cat# 2208, RRID: AB_2238543 Mouse monoclonal anti-γH2AX Ser139 (Clone JBW301) Sigma-Aldrich (EMD Millipore) Cat# 05–636, RRID: AB_309864 Mouse monoclonal anti-DNA Polymerase iota (POLI, Clone OTI1H9) Abcam Cat# ab157244 Mouse monoclonal BV421-conjugated antiCD24 (Clone ML5) BD Biosciences Cat# 562789, RRID: AB_2737796 Mouse monoclonal Alexa Fluor 647-conjugated ani-EpCAM (Clone VU1D9) Cell Signaling Technology Cat# 5447, RRID: AB_10693458 Rabbit monoclonal anti-BRCA2 (Clone D9S6V) Cell Signaling Technology Cat# 10741, RRID: AB_2797730 Rabbit monoclonal anti-RAD52 (Clone EPR3464(2)) Abcam Cat# ab124971, RRID: AB_10971685 Mouse monoclonal anti-β-Actin (Clone 8H10D10) Cell Signaling Technology Cat# 3700, RRID: AB_2242334 Rabbit monoclonal anti-Vinculin (Clone E1E9V) Cell Signaling Technology Cat# 13901, RRID: AB_2728768 Mouse monoclonal anti-BrdU (Clone B44) BD Biosciences Cat# 347580, RRID: AB_400326 Rat monoclonal anti-BrdU (Clone BU1/75 (ICR1)) Abcam Cat# ab6326, RRID: AB_305426 Biological samples Human breast tissues from prophylactic mastectomies of BRCA2 mutation carriers Tissue Procurement Core, Cleveland Clinic N/A Chemicals, peptides, and recombinant proteins Aphidicolin Sigma-Aldrich Cat# 5047440001 Colcemid Gibco Cat# 15210 Potassium chloride Sigma Cat# P9327 Giemsa Sigma-Aldrich Cat# 32884 Cytochalasin-B Sigma Cat# C2743 CIdU (5-Chloro-2′-deoxyuridine) Sigma-Aldrich Cat# C6891 IdU (5-Iodo-2′-deoxyuridine) Sigma-Aldrich Cat# I7125 Lipofectamine RNAiMAX ThermoFisher Scientific Cat# 13778075 DAPI Invitrogen Cat# P36962 DMEM/F12 media ThermoFisher Scientific Cat# 11330057 Horse Serum ThermoFisher Scientific Cat# 16050122 Insulin Sigma Cat# I-1882 EGF Peprotech Cat# AF-100–15 Hydrocortisone Sigma Cat# H6909 (Continued on next page) 12 iScience 29, 117830, November 20, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Cholera toxin Sigma Aldrich Cat# C8052 Mammary Epithelial Cell Basal Medium ATCC Cat# PCS-600–030, Mammary Epithelial Cell Growth Kit ATCC Cat# PCS-600–040 DMEM/F-12 GlutaMAX ThermoFisher Scientific Cat# 10565018 L-glutamine ThermoFisher Scientific Cat# 25030081 Gentamycin/amphotericin-B ThermoFisher Scientific Cat# R01510 Critical commercial assays Click-iT EdU Alexa Fluor 488 flow cytometry assay kit Invitrogen Cat# C10425 Click-iT EdU Alexa Fluor 488 Imaging kit Invitrogen Cat# C10637 FxCycle Violet Ready Flow Reagent Invitrogen Cat# R37166 Live-or-Dye 510/551 Biotium Cat # 32012A MycoAlert Mycoplasma Detection Kit Lonza Cat# LT07–218 PierceTM Rapid Gold BCA Protein Assay Kit Thermo Scientific, Cat# A53225 RIPA buffer Cell Signaling Technology Cat# 9803/9806 Protease and phosphatase inhibitors Cell Signaling Technology Cat# 5872 PMSF Cell Signaling Technology Cat# 8553 SDS-PAGE Precast Protein Gels Bio-Rad Cat# 4561084 Clarity Max Western ECL Substrate Bio-Rad Cat# 1705062 Experimental models: Cell lines Human mammary epithelial cell line: MCF10A Cleveland Clinic Research, cell and media production core facility Cat# ATCC, CRL-10317 Primary Human Mammary Epithelial Cells: HMECs American Type Culture Collection (ATCC) Cat# ATCC, PCS-600–010 Primary BRCA2-mutant Human Mammary Epithelial Cells: BRCA2mut/- HMECs Tissue Procurement Core, Cleveland Clinic Caputo et al.55 Human mammary cancer cell line: MCF7 Cleveland Clinic Research, cell and media production core facility Cat# ATCC, HTB-22 Human cervical cancer cell line: HeLa Cleveland Clinic Research, cell and media production core facility Cat# ATCC, CRM-CCL-2 Oligonucleotides siRNA sequences Dharmacon Horizon Discovery Supplementary file, this paper Software and algorithms R (version 4.5.3) R Software https://www.r-project.org/ Seurat (version 5.4.0) Hao, Y. et al., Dictionary learning for integrative, multimodal and scalable singlecell analysis. ..

    Imaging:

    Article Title: Polymerase iota coordinates adaptation to replication stress in BRCA2-deficient mammary epithelial cells.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-FANCD2 (Clone EPR2302) Abcam Cat# ab108928, RRID: AB_10862535 Mouse monoclonal anti-phospho-Histone H3 Ser10 (Clone 6G3) Cell Signaling Technology Cat# 9706, RRID: AB_331748 Mouse monoclonal anti-Cyclin A2 (Clone 1C6C11) Proteintech Cat# 66391-1-Ig, RRID: AB_2881767 Rabbit polyclonal anti-53BP1 Cell Signaling Technology Cat# 4937, RRID: AB_10694558 Rat monoclonal anti-RPA32/RPA2 (Clone 4E4) Cell Signaling Technology Cat# 2208, RRID: AB_2238543 Mouse monoclonal anti-γH2AX Ser139 (Clone JBW301) Sigma-Aldrich (EMD Millipore) Cat# 05–636, RRID: AB_309864 Mouse monoclonal anti-DNA Polymerase iota (POLI, Clone OTI1H9) Abcam Cat# ab157244 Mouse monoclonal BV421-conjugated antiCD24 (Clone ML5) BD Biosciences Cat# 562789, RRID: AB_2737796 Mouse monoclonal Alexa Fluor 647-conjugated ani-EpCAM (Clone VU1D9) Cell Signaling Technology Cat# 5447, RRID: AB_10693458 Rabbit monoclonal anti-BRCA2 (Clone D9S6V) Cell Signaling Technology Cat# 10741, RRID: AB_2797730 Rabbit monoclonal anti-RAD52 (Clone EPR3464(2)) Abcam Cat# ab124971, RRID: AB_10971685 Mouse monoclonal anti-β-Actin (Clone 8H10D10) Cell Signaling Technology Cat# 3700, RRID: AB_2242334 Rabbit monoclonal anti-Vinculin (Clone E1E9V) Cell Signaling Technology Cat# 13901, RRID: AB_2728768 Mouse monoclonal anti-BrdU (Clone B44) BD Biosciences Cat# 347580, RRID: AB_400326 Rat monoclonal anti-BrdU (Clone BU1/75 (ICR1)) Abcam Cat# ab6326, RRID: AB_305426 Biological samples Human breast tissues from prophylactic mastectomies of BRCA2 mutation carriers Tissue Procurement Core, Cleveland Clinic N/A Chemicals, peptides, and recombinant proteins Aphidicolin Sigma-Aldrich Cat# 5047440001 Colcemid Gibco Cat# 15210 Potassium chloride Sigma Cat# P9327 Giemsa Sigma-Aldrich Cat# 32884 Cytochalasin-B Sigma Cat# C2743 CIdU (5-Chloro-2′-deoxyuridine) Sigma-Aldrich Cat# C6891 IdU (5-Iodo-2′-deoxyuridine) Sigma-Aldrich Cat# I7125 Lipofectamine RNAiMAX ThermoFisher Scientific Cat# 13778075 DAPI Invitrogen Cat# P36962 DMEM/F12 media ThermoFisher Scientific Cat# 11330057 Horse Serum ThermoFisher Scientific Cat# 16050122 Insulin Sigma Cat# I-1882 EGF Peprotech Cat# AF-100–15 Hydrocortisone Sigma Cat# H6909 (Continued on next page) 12 iScience 29, 117830, November 20, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Cholera toxin Sigma Aldrich Cat# C8052 Mammary Epithelial Cell Basal Medium ATCC Cat# PCS-600–030, Mammary Epithelial Cell Growth Kit ATCC Cat# PCS-600–040 DMEM/F-12 GlutaMAX ThermoFisher Scientific Cat# 10565018 L-glutamine ThermoFisher Scientific Cat# 25030081 Gentamycin/amphotericin-B ThermoFisher Scientific Cat# R01510 Critical commercial assays Click-iT EdU Alexa Fluor 488 flow cytometry assay kit Invitrogen Cat# C10425 Click-iT EdU Alexa Fluor 488 Imaging kit Invitrogen Cat# C10637 FxCycle Violet Ready Flow Reagent Invitrogen Cat# R37166 Live-or-Dye 510/551 Biotium Cat # 32012A MycoAlert Mycoplasma Detection Kit Lonza Cat# LT07–218 PierceTM Rapid Gold BCA Protein Assay Kit Thermo Scientific, Cat# A53225 RIPA buffer Cell Signaling Technology Cat# 9803/9806 Protease and phosphatase inhibitors Cell Signaling Technology Cat# 5872 PMSF Cell Signaling Technology Cat# 8553 SDS-PAGE Precast Protein Gels Bio-Rad Cat# 4561084 Clarity Max Western ECL Substrate Bio-Rad Cat# 1705062 Experimental models: Cell lines Human mammary epithelial cell line: MCF10A Cleveland Clinic Research, cell and media production core facility Cat# ATCC, CRL-10317 Primary Human Mammary Epithelial Cells: HMECs American Type Culture Collection (ATCC) Cat# ATCC, PCS-600–010 Primary BRCA2-mutant Human Mammary Epithelial Cells: BRCA2mut/- HMECs Tissue Procurement Core, Cleveland Clinic Caputo et al.55 Human mammary cancer cell line: MCF7 Cleveland Clinic Research, cell and media production core facility Cat# ATCC, HTB-22 Human cervical cancer cell line: HeLa Cleveland Clinic Research, cell and media production core facility Cat# ATCC, CRM-CCL-2 Oligonucleotides siRNA sequences Dharmacon Horizon Discovery Supplementary file, this paper Software and algorithms R (version 4.5.3) R Software https://www.r-project.org/ Seurat (version 5.4.0) Hao, Y. et al., Dictionary learning for integrative, multimodal and scalable singlecell analysis. ..

    BIA-KA:

    Article Title: Polymerase iota coordinates adaptation to replication stress in BRCA2-deficient mammary epithelial cells.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-FANCD2 (Clone EPR2302) Abcam Cat# ab108928, RRID: AB_10862535 Mouse monoclonal anti-phospho-Histone H3 Ser10 (Clone 6G3) Cell Signaling Technology Cat# 9706, RRID: AB_331748 Mouse monoclonal anti-Cyclin A2 (Clone 1C6C11) Proteintech Cat# 66391-1-Ig, RRID: AB_2881767 Rabbit polyclonal anti-53BP1 Cell Signaling Technology Cat# 4937, RRID: AB_10694558 Rat monoclonal anti-RPA32/RPA2 (Clone 4E4) Cell Signaling Technology Cat# 2208, RRID: AB_2238543 Mouse monoclonal anti-γH2AX Ser139 (Clone JBW301) Sigma-Aldrich (EMD Millipore) Cat# 05–636, RRID: AB_309864 Mouse monoclonal anti-DNA Polymerase iota (POLI, Clone OTI1H9) Abcam Cat# ab157244 Mouse monoclonal BV421-conjugated antiCD24 (Clone ML5) BD Biosciences Cat# 562789, RRID: AB_2737796 Mouse monoclonal Alexa Fluor 647-conjugated ani-EpCAM (Clone VU1D9) Cell Signaling Technology Cat# 5447, RRID: AB_10693458 Rabbit monoclonal anti-BRCA2 (Clone D9S6V) Cell Signaling Technology Cat# 10741, RRID: AB_2797730 Rabbit monoclonal anti-RAD52 (Clone EPR3464(2)) Abcam Cat# ab124971, RRID: AB_10971685 Mouse monoclonal anti-β-Actin (Clone 8H10D10) Cell Signaling Technology Cat# 3700, RRID: AB_2242334 Rabbit monoclonal anti-Vinculin (Clone E1E9V) Cell Signaling Technology Cat# 13901, RRID: AB_2728768 Mouse monoclonal anti-BrdU (Clone B44) BD Biosciences Cat# 347580, RRID: AB_400326 Rat monoclonal anti-BrdU (Clone BU1/75 (ICR1)) Abcam Cat# ab6326, RRID: AB_305426 Biological samples Human breast tissues from prophylactic mastectomies of BRCA2 mutation carriers Tissue Procurement Core, Cleveland Clinic N/A Chemicals, peptides, and recombinant proteins Aphidicolin Sigma-Aldrich Cat# 5047440001 Colcemid Gibco Cat# 15210 Potassium chloride Sigma Cat# P9327 Giemsa Sigma-Aldrich Cat# 32884 Cytochalasin-B Sigma Cat# C2743 CIdU (5-Chloro-2′-deoxyuridine) Sigma-Aldrich Cat# C6891 IdU (5-Iodo-2′-deoxyuridine) Sigma-Aldrich Cat# I7125 Lipofectamine RNAiMAX ThermoFisher Scientific Cat# 13778075 DAPI Invitrogen Cat# P36962 DMEM/F12 media ThermoFisher Scientific Cat# 11330057 Horse Serum ThermoFisher Scientific Cat# 16050122 Insulin Sigma Cat# I-1882 EGF Peprotech Cat# AF-100–15 Hydrocortisone Sigma Cat# H6909 (Continued on next page) 12 iScience 29, 117830, November 20, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Cholera toxin Sigma Aldrich Cat# C8052 Mammary Epithelial Cell Basal Medium ATCC Cat# PCS-600–030, Mammary Epithelial Cell Growth Kit ATCC Cat# PCS-600–040 DMEM/F-12 GlutaMAX ThermoFisher Scientific Cat# 10565018 L-glutamine ThermoFisher Scientific Cat# 25030081 Gentamycin/amphotericin-B ThermoFisher Scientific Cat# R01510 Critical commercial assays Click-iT EdU Alexa Fluor 488 flow cytometry assay kit Invitrogen Cat# C10425 Click-iT EdU Alexa Fluor 488 Imaging kit Invitrogen Cat# C10637 FxCycle Violet Ready Flow Reagent Invitrogen Cat# R37166 Live-or-Dye 510/551 Biotium Cat # 32012A MycoAlert Mycoplasma Detection Kit Lonza Cat# LT07–218 PierceTM Rapid Gold BCA Protein Assay Kit Thermo Scientific, Cat# A53225 RIPA buffer Cell Signaling Technology Cat# 9803/9806 Protease and phosphatase inhibitors Cell Signaling Technology Cat# 5872 PMSF Cell Signaling Technology Cat# 8553 SDS-PAGE Precast Protein Gels Bio-Rad Cat# 4561084 Clarity Max Western ECL Substrate Bio-Rad Cat# 1705062 Experimental models: Cell lines Human mammary epithelial cell line: MCF10A Cleveland Clinic Research, cell and media production core facility Cat# ATCC, CRL-10317 Primary Human Mammary Epithelial Cells: HMECs American Type Culture Collection (ATCC) Cat# ATCC, PCS-600–010 Primary BRCA2-mutant Human Mammary Epithelial Cells: BRCA2mut/- HMECs Tissue Procurement Core, Cleveland Clinic Caputo et al.55 Human mammary cancer cell line: MCF7 Cleveland Clinic Research, cell and media production core facility Cat# ATCC, HTB-22 Human cervical cancer cell line: HeLa Cleveland Clinic Research, cell and media production core facility Cat# ATCC, CRM-CCL-2 Oligonucleotides siRNA sequences Dharmacon Horizon Discovery Supplementary file, this paper Software and algorithms R (version 4.5.3) R Software https://www.r-project.org/ Seurat (version 5.4.0) Hao, Y. et al., Dictionary learning for integrative, multimodal and scalable singlecell analysis. ..

    Cell-Signaling:

    Article Title: Polymerase iota coordinates adaptation to replication stress in BRCA2-deficient mammary epithelial cells.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-FANCD2 (Clone EPR2302) Abcam Cat# ab108928, RRID: AB_10862535 Mouse monoclonal anti-phospho-Histone H3 Ser10 (Clone 6G3) Cell Signaling Technology Cat# 9706, RRID: AB_331748 Mouse monoclonal anti-Cyclin A2 (Clone 1C6C11) Proteintech Cat# 66391-1-Ig, RRID: AB_2881767 Rabbit polyclonal anti-53BP1 Cell Signaling Technology Cat# 4937, RRID: AB_10694558 Rat monoclonal anti-RPA32/RPA2 (Clone 4E4) Cell Signaling Technology Cat# 2208, RRID: AB_2238543 Mouse monoclonal anti-γH2AX Ser139 (Clone JBW301) Sigma-Aldrich (EMD Millipore) Cat# 05–636, RRID: AB_309864 Mouse monoclonal anti-DNA Polymerase iota (POLI, Clone OTI1H9) Abcam Cat# ab157244 Mouse monoclonal BV421-conjugated antiCD24 (Clone ML5) BD Biosciences Cat# 562789, RRID: AB_2737796 Mouse monoclonal Alexa Fluor 647-conjugated ani-EpCAM (Clone VU1D9) Cell Signaling Technology Cat# 5447, RRID: AB_10693458 Rabbit monoclonal anti-BRCA2 (Clone D9S6V) Cell Signaling Technology Cat# 10741, RRID: AB_2797730 Rabbit monoclonal anti-RAD52 (Clone EPR3464(2)) Abcam Cat# ab124971, RRID: AB_10971685 Mouse monoclonal anti-β-Actin (Clone 8H10D10) Cell Signaling Technology Cat# 3700, RRID: AB_2242334 Rabbit monoclonal anti-Vinculin (Clone E1E9V) Cell Signaling Technology Cat# 13901, RRID: AB_2728768 Mouse monoclonal anti-BrdU (Clone B44) BD Biosciences Cat# 347580, RRID: AB_400326 Rat monoclonal anti-BrdU (Clone BU1/75 (ICR1)) Abcam Cat# ab6326, RRID: AB_305426 Biological samples Human breast tissues from prophylactic mastectomies of BRCA2 mutation carriers Tissue Procurement Core, Cleveland Clinic N/A Chemicals, peptides, and recombinant proteins Aphidicolin Sigma-Aldrich Cat# 5047440001 Colcemid Gibco Cat# 15210 Potassium chloride Sigma Cat# P9327 Giemsa Sigma-Aldrich Cat# 32884 Cytochalasin-B Sigma Cat# C2743 CIdU (5-Chloro-2′-deoxyuridine) Sigma-Aldrich Cat# C6891 IdU (5-Iodo-2′-deoxyuridine) Sigma-Aldrich Cat# I7125 Lipofectamine RNAiMAX ThermoFisher Scientific Cat# 13778075 DAPI Invitrogen Cat# P36962 DMEM/F12 media ThermoFisher Scientific Cat# 11330057 Horse Serum ThermoFisher Scientific Cat# 16050122 Insulin Sigma Cat# I-1882 EGF Peprotech Cat# AF-100–15 Hydrocortisone Sigma Cat# H6909 (Continued on next page) 12 iScience 29, 117830, November 20, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Cholera toxin Sigma Aldrich Cat# C8052 Mammary Epithelial Cell Basal Medium ATCC Cat# PCS-600–030, Mammary Epithelial Cell Growth Kit ATCC Cat# PCS-600–040 DMEM/F-12 GlutaMAX ThermoFisher Scientific Cat# 10565018 L-glutamine ThermoFisher Scientific Cat# 25030081 Gentamycin/amphotericin-B ThermoFisher Scientific Cat# R01510 Critical commercial assays Click-iT EdU Alexa Fluor 488 flow cytometry assay kit Invitrogen Cat# C10425 Click-iT EdU Alexa Fluor 488 Imaging kit Invitrogen Cat# C10637 FxCycle Violet Ready Flow Reagent Invitrogen Cat# R37166 Live-or-Dye 510/551 Biotium Cat # 32012A MycoAlert Mycoplasma Detection Kit Lonza Cat# LT07–218 PierceTM Rapid Gold BCA Protein Assay Kit Thermo Scientific, Cat# A53225 RIPA buffer Cell Signaling Technology Cat# 9803/9806 Protease and phosphatase inhibitors Cell Signaling Technology Cat# 5872 PMSF Cell Signaling Technology Cat# 8553 SDS-PAGE Precast Protein Gels Bio-Rad Cat# 4561084 Clarity Max Western ECL Substrate Bio-Rad Cat# 1705062 Experimental models: Cell lines Human mammary epithelial cell line: MCF10A Cleveland Clinic Research, cell and media production core facility Cat# ATCC, CRL-10317 Primary Human Mammary Epithelial Cells: HMECs American Type Culture Collection (ATCC) Cat# ATCC, PCS-600–010 Primary BRCA2-mutant Human Mammary Epithelial Cells: BRCA2mut/- HMECs Tissue Procurement Core, Cleveland Clinic Caputo et al.55 Human mammary cancer cell line: MCF7 Cleveland Clinic Research, cell and media production core facility Cat# ATCC, HTB-22 Human cervical cancer cell line: HeLa Cleveland Clinic Research, cell and media production core facility Cat# ATCC, CRM-CCL-2 Oligonucleotides siRNA sequences Dharmacon Horizon Discovery Supplementary file, this paper Software and algorithms R (version 4.5.3) R Software https://www.r-project.org/ Seurat (version 5.4.0) Hao, Y. et al., Dictionary learning for integrative, multimodal and scalable singlecell analysis. ..

    SDS Page:

    Article Title: Polymerase iota coordinates adaptation to replication stress in BRCA2-deficient mammary epithelial cells.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-FANCD2 (Clone EPR2302) Abcam Cat# ab108928, RRID: AB_10862535 Mouse monoclonal anti-phospho-Histone H3 Ser10 (Clone 6G3) Cell Signaling Technology Cat# 9706, RRID: AB_331748 Mouse monoclonal anti-Cyclin A2 (Clone 1C6C11) Proteintech Cat# 66391-1-Ig, RRID: AB_2881767 Rabbit polyclonal anti-53BP1 Cell Signaling Technology Cat# 4937, RRID: AB_10694558 Rat monoclonal anti-RPA32/RPA2 (Clone 4E4) Cell Signaling Technology Cat# 2208, RRID: AB_2238543 Mouse monoclonal anti-γH2AX Ser139 (Clone JBW301) Sigma-Aldrich (EMD Millipore) Cat# 05–636, RRID: AB_309864 Mouse monoclonal anti-DNA Polymerase iota (POLI, Clone OTI1H9) Abcam Cat# ab157244 Mouse monoclonal BV421-conjugated antiCD24 (Clone ML5) BD Biosciences Cat# 562789, RRID: AB_2737796 Mouse monoclonal Alexa Fluor 647-conjugated ani-EpCAM (Clone VU1D9) Cell Signaling Technology Cat# 5447, RRID: AB_10693458 Rabbit monoclonal anti-BRCA2 (Clone D9S6V) Cell Signaling Technology Cat# 10741, RRID: AB_2797730 Rabbit monoclonal anti-RAD52 (Clone EPR3464(2)) Abcam Cat# ab124971, RRID: AB_10971685 Mouse monoclonal anti-β-Actin (Clone 8H10D10) Cell Signaling Technology Cat# 3700, RRID: AB_2242334 Rabbit monoclonal anti-Vinculin (Clone E1E9V) Cell Signaling Technology Cat# 13901, RRID: AB_2728768 Mouse monoclonal anti-BrdU (Clone B44) BD Biosciences Cat# 347580, RRID: AB_400326 Rat monoclonal anti-BrdU (Clone BU1/75 (ICR1)) Abcam Cat# ab6326, RRID: AB_305426 Biological samples Human breast tissues from prophylactic mastectomies of BRCA2 mutation carriers Tissue Procurement Core, Cleveland Clinic N/A Chemicals, peptides, and recombinant proteins Aphidicolin Sigma-Aldrich Cat# 5047440001 Colcemid Gibco Cat# 15210 Potassium chloride Sigma Cat# P9327 Giemsa Sigma-Aldrich Cat# 32884 Cytochalasin-B Sigma Cat# C2743 CIdU (5-Chloro-2′-deoxyuridine) Sigma-Aldrich Cat# C6891 IdU (5-Iodo-2′-deoxyuridine) Sigma-Aldrich Cat# I7125 Lipofectamine RNAiMAX ThermoFisher Scientific Cat# 13778075 DAPI Invitrogen Cat# P36962 DMEM/F12 media ThermoFisher Scientific Cat# 11330057 Horse Serum ThermoFisher Scientific Cat# 16050122 Insulin Sigma Cat# I-1882 EGF Peprotech Cat# AF-100–15 Hydrocortisone Sigma Cat# H6909 (Continued on next page) 12 iScience 29, 117830, November 20, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Cholera toxin Sigma Aldrich Cat# C8052 Mammary Epithelial Cell Basal Medium ATCC Cat# PCS-600–030, Mammary Epithelial Cell Growth Kit ATCC Cat# PCS-600–040 DMEM/F-12 GlutaMAX ThermoFisher Scientific Cat# 10565018 L-glutamine ThermoFisher Scientific Cat# 25030081 Gentamycin/amphotericin-B ThermoFisher Scientific Cat# R01510 Critical commercial assays Click-iT EdU Alexa Fluor 488 flow cytometry assay kit Invitrogen Cat# C10425 Click-iT EdU Alexa Fluor 488 Imaging kit Invitrogen Cat# C10637 FxCycle Violet Ready Flow Reagent Invitrogen Cat# R37166 Live-or-Dye 510/551 Biotium Cat # 32012A MycoAlert Mycoplasma Detection Kit Lonza Cat# LT07–218 PierceTM Rapid Gold BCA Protein Assay Kit Thermo Scientific, Cat# A53225 RIPA buffer Cell Signaling Technology Cat# 9803/9806 Protease and phosphatase inhibitors Cell Signaling Technology Cat# 5872 PMSF Cell Signaling Technology Cat# 8553 SDS-PAGE Precast Protein Gels Bio-Rad Cat# 4561084 Clarity Max Western ECL Substrate Bio-Rad Cat# 1705062 Experimental models: Cell lines Human mammary epithelial cell line: MCF10A Cleveland Clinic Research, cell and media production core facility Cat# ATCC, CRL-10317 Primary Human Mammary Epithelial Cells: HMECs American Type Culture Collection (ATCC) Cat# ATCC, PCS-600–010 Primary BRCA2-mutant Human Mammary Epithelial Cells: BRCA2mut/- HMECs Tissue Procurement Core, Cleveland Clinic Caputo et al.55 Human mammary cancer cell line: MCF7 Cleveland Clinic Research, cell and media production core facility Cat# ATCC, HTB-22 Human cervical cancer cell line: HeLa Cleveland Clinic Research, cell and media production core facility Cat# ATCC, CRM-CCL-2 Oligonucleotides siRNA sequences Dharmacon Horizon Discovery Supplementary file, this paper Software and algorithms R (version 4.5.3) R Software https://www.r-project.org/ Seurat (version 5.4.0) Hao, Y. et al., Dictionary learning for integrative, multimodal and scalable singlecell analysis. ..

    Western Blot:

    Article Title: Polymerase iota coordinates adaptation to replication stress in BRCA2-deficient mammary epithelial cells.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-FANCD2 (Clone EPR2302) Abcam Cat# ab108928, RRID: AB_10862535 Mouse monoclonal anti-phospho-Histone H3 Ser10 (Clone 6G3) Cell Signaling Technology Cat# 9706, RRID: AB_331748 Mouse monoclonal anti-Cyclin A2 (Clone 1C6C11) Proteintech Cat# 66391-1-Ig, RRID: AB_2881767 Rabbit polyclonal anti-53BP1 Cell Signaling Technology Cat# 4937, RRID: AB_10694558 Rat monoclonal anti-RPA32/RPA2 (Clone 4E4) Cell Signaling Technology Cat# 2208, RRID: AB_2238543 Mouse monoclonal anti-γH2AX Ser139 (Clone JBW301) Sigma-Aldrich (EMD Millipore) Cat# 05–636, RRID: AB_309864 Mouse monoclonal anti-DNA Polymerase iota (POLI, Clone OTI1H9) Abcam Cat# ab157244 Mouse monoclonal BV421-conjugated antiCD24 (Clone ML5) BD Biosciences Cat# 562789, RRID: AB_2737796 Mouse monoclonal Alexa Fluor 647-conjugated ani-EpCAM (Clone VU1D9) Cell Signaling Technology Cat# 5447, RRID: AB_10693458 Rabbit monoclonal anti-BRCA2 (Clone D9S6V) Cell Signaling Technology Cat# 10741, RRID: AB_2797730 Rabbit monoclonal anti-RAD52 (Clone EPR3464(2)) Abcam Cat# ab124971, RRID: AB_10971685 Mouse monoclonal anti-β-Actin (Clone 8H10D10) Cell Signaling Technology Cat# 3700, RRID: AB_2242334 Rabbit monoclonal anti-Vinculin (Clone E1E9V) Cell Signaling Technology Cat# 13901, RRID: AB_2728768 Mouse monoclonal anti-BrdU (Clone B44) BD Biosciences Cat# 347580, RRID: AB_400326 Rat monoclonal anti-BrdU (Clone BU1/75 (ICR1)) Abcam Cat# ab6326, RRID: AB_305426 Biological samples Human breast tissues from prophylactic mastectomies of BRCA2 mutation carriers Tissue Procurement Core, Cleveland Clinic N/A Chemicals, peptides, and recombinant proteins Aphidicolin Sigma-Aldrich Cat# 5047440001 Colcemid Gibco Cat# 15210 Potassium chloride Sigma Cat# P9327 Giemsa Sigma-Aldrich Cat# 32884 Cytochalasin-B Sigma Cat# C2743 CIdU (5-Chloro-2′-deoxyuridine) Sigma-Aldrich Cat# C6891 IdU (5-Iodo-2′-deoxyuridine) Sigma-Aldrich Cat# I7125 Lipofectamine RNAiMAX ThermoFisher Scientific Cat# 13778075 DAPI Invitrogen Cat# P36962 DMEM/F12 media ThermoFisher Scientific Cat# 11330057 Horse Serum ThermoFisher Scientific Cat# 16050122 Insulin Sigma Cat# I-1882 EGF Peprotech Cat# AF-100–15 Hydrocortisone Sigma Cat# H6909 (Continued on next page) 12 iScience 29, 117830, November 20, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Cholera toxin Sigma Aldrich Cat# C8052 Mammary Epithelial Cell Basal Medium ATCC Cat# PCS-600–030, Mammary Epithelial Cell Growth Kit ATCC Cat# PCS-600–040 DMEM/F-12 GlutaMAX ThermoFisher Scientific Cat# 10565018 L-glutamine ThermoFisher Scientific Cat# 25030081 Gentamycin/amphotericin-B ThermoFisher Scientific Cat# R01510 Critical commercial assays Click-iT EdU Alexa Fluor 488 flow cytometry assay kit Invitrogen Cat# C10425 Click-iT EdU Alexa Fluor 488 Imaging kit Invitrogen Cat# C10637 FxCycle Violet Ready Flow Reagent Invitrogen Cat# R37166 Live-or-Dye 510/551 Biotium Cat # 32012A MycoAlert Mycoplasma Detection Kit Lonza Cat# LT07–218 PierceTM Rapid Gold BCA Protein Assay Kit Thermo Scientific, Cat# A53225 RIPA buffer Cell Signaling Technology Cat# 9803/9806 Protease and phosphatase inhibitors Cell Signaling Technology Cat# 5872 PMSF Cell Signaling Technology Cat# 8553 SDS-PAGE Precast Protein Gels Bio-Rad Cat# 4561084 Clarity Max Western ECL Substrate Bio-Rad Cat# 1705062 Experimental models: Cell lines Human mammary epithelial cell line: MCF10A Cleveland Clinic Research, cell and media production core facility Cat# ATCC, CRL-10317 Primary Human Mammary Epithelial Cells: HMECs American Type Culture Collection (ATCC) Cat# ATCC, PCS-600–010 Primary BRCA2-mutant Human Mammary Epithelial Cells: BRCA2mut/- HMECs Tissue Procurement Core, Cleveland Clinic Caputo et al.55 Human mammary cancer cell line: MCF7 Cleveland Clinic Research, cell and media production core facility Cat# ATCC, HTB-22 Human cervical cancer cell line: HeLa Cleveland Clinic Research, cell and media production core facility Cat# ATCC, CRM-CCL-2 Oligonucleotides siRNA sequences Dharmacon Horizon Discovery Supplementary file, this paper Software and algorithms R (version 4.5.3) R Software https://www.r-project.org/ Seurat (version 5.4.0) Hao, Y. et al., Dictionary learning for integrative, multimodal and scalable singlecell analysis. ..

    Tissue:

    Article Title: Polymerase iota coordinates adaptation to replication stress in BRCA2-deficient mammary epithelial cells.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-FANCD2 (Clone EPR2302) Abcam Cat# ab108928, RRID: AB_10862535 Mouse monoclonal anti-phospho-Histone H3 Ser10 (Clone 6G3) Cell Signaling Technology Cat# 9706, RRID: AB_331748 Mouse monoclonal anti-Cyclin A2 (Clone 1C6C11) Proteintech Cat# 66391-1-Ig, RRID: AB_2881767 Rabbit polyclonal anti-53BP1 Cell Signaling Technology Cat# 4937, RRID: AB_10694558 Rat monoclonal anti-RPA32/RPA2 (Clone 4E4) Cell Signaling Technology Cat# 2208, RRID: AB_2238543 Mouse monoclonal anti-γH2AX Ser139 (Clone JBW301) Sigma-Aldrich (EMD Millipore) Cat# 05–636, RRID: AB_309864 Mouse monoclonal anti-DNA Polymerase iota (POLI, Clone OTI1H9) Abcam Cat# ab157244 Mouse monoclonal BV421-conjugated antiCD24 (Clone ML5) BD Biosciences Cat# 562789, RRID: AB_2737796 Mouse monoclonal Alexa Fluor 647-conjugated ani-EpCAM (Clone VU1D9) Cell Signaling Technology Cat# 5447, RRID: AB_10693458 Rabbit monoclonal anti-BRCA2 (Clone D9S6V) Cell Signaling Technology Cat# 10741, RRID: AB_2797730 Rabbit monoclonal anti-RAD52 (Clone EPR3464(2)) Abcam Cat# ab124971, RRID: AB_10971685 Mouse monoclonal anti-β-Actin (Clone 8H10D10) Cell Signaling Technology Cat# 3700, RRID: AB_2242334 Rabbit monoclonal anti-Vinculin (Clone E1E9V) Cell Signaling Technology Cat# 13901, RRID: AB_2728768 Mouse monoclonal anti-BrdU (Clone B44) BD Biosciences Cat# 347580, RRID: AB_400326 Rat monoclonal anti-BrdU (Clone BU1/75 (ICR1)) Abcam Cat# ab6326, RRID: AB_305426 Biological samples Human breast tissues from prophylactic mastectomies of BRCA2 mutation carriers Tissue Procurement Core, Cleveland Clinic N/A Chemicals, peptides, and recombinant proteins Aphidicolin Sigma-Aldrich Cat# 5047440001 Colcemid Gibco Cat# 15210 Potassium chloride Sigma Cat# P9327 Giemsa Sigma-Aldrich Cat# 32884 Cytochalasin-B Sigma Cat# C2743 CIdU (5-Chloro-2′-deoxyuridine) Sigma-Aldrich Cat# C6891 IdU (5-Iodo-2′-deoxyuridine) Sigma-Aldrich Cat# I7125 Lipofectamine RNAiMAX ThermoFisher Scientific Cat# 13778075 DAPI Invitrogen Cat# P36962 DMEM/F12 media ThermoFisher Scientific Cat# 11330057 Horse Serum ThermoFisher Scientific Cat# 16050122 Insulin Sigma Cat# I-1882 EGF Peprotech Cat# AF-100–15 Hydrocortisone Sigma Cat# H6909 (Continued on next page) 12 iScience 29, 117830, November 20, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Cholera toxin Sigma Aldrich Cat# C8052 Mammary Epithelial Cell Basal Medium ATCC Cat# PCS-600–030, Mammary Epithelial Cell Growth Kit ATCC Cat# PCS-600–040 DMEM/F-12 GlutaMAX ThermoFisher Scientific Cat# 10565018 L-glutamine ThermoFisher Scientific Cat# 25030081 Gentamycin/amphotericin-B ThermoFisher Scientific Cat# R01510 Critical commercial assays Click-iT EdU Alexa Fluor 488 flow cytometry assay kit Invitrogen Cat# C10425 Click-iT EdU Alexa Fluor 488 Imaging kit Invitrogen Cat# C10637 FxCycle Violet Ready Flow Reagent Invitrogen Cat# R37166 Live-or-Dye 510/551 Biotium Cat # 32012A MycoAlert Mycoplasma Detection Kit Lonza Cat# LT07–218 PierceTM Rapid Gold BCA Protein Assay Kit Thermo Scientific, Cat# A53225 RIPA buffer Cell Signaling Technology Cat# 9803/9806 Protease and phosphatase inhibitors Cell Signaling Technology Cat# 5872 PMSF Cell Signaling Technology Cat# 8553 SDS-PAGE Precast Protein Gels Bio-Rad Cat# 4561084 Clarity Max Western ECL Substrate Bio-Rad Cat# 1705062 Experimental models: Cell lines Human mammary epithelial cell line: MCF10A Cleveland Clinic Research, cell and media production core facility Cat# ATCC, CRL-10317 Primary Human Mammary Epithelial Cells: HMECs American Type Culture Collection (ATCC) Cat# ATCC, PCS-600–010 Primary BRCA2-mutant Human Mammary Epithelial Cells: BRCA2mut/- HMECs Tissue Procurement Core, Cleveland Clinic Caputo et al.55 Human mammary cancer cell line: MCF7 Cleveland Clinic Research, cell and media production core facility Cat# ATCC, HTB-22 Human cervical cancer cell line: HeLa Cleveland Clinic Research, cell and media production core facility Cat# ATCC, CRM-CCL-2 Oligonucleotides siRNA sequences Dharmacon Horizon Discovery Supplementary file, this paper Software and algorithms R (version 4.5.3) R Software https://www.r-project.org/ Seurat (version 5.4.0) Hao, Y. et al., Dictionary learning for integrative, multimodal and scalable singlecell analysis. ..

    Software:

    Article Title: Polymerase iota coordinates adaptation to replication stress in BRCA2-deficient mammary epithelial cells.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-FANCD2 (Clone EPR2302) Abcam Cat# ab108928, RRID: AB_10862535 Mouse monoclonal anti-phospho-Histone H3 Ser10 (Clone 6G3) Cell Signaling Technology Cat# 9706, RRID: AB_331748 Mouse monoclonal anti-Cyclin A2 (Clone 1C6C11) Proteintech Cat# 66391-1-Ig, RRID: AB_2881767 Rabbit polyclonal anti-53BP1 Cell Signaling Technology Cat# 4937, RRID: AB_10694558 Rat monoclonal anti-RPA32/RPA2 (Clone 4E4) Cell Signaling Technology Cat# 2208, RRID: AB_2238543 Mouse monoclonal anti-γH2AX Ser139 (Clone JBW301) Sigma-Aldrich (EMD Millipore) Cat# 05–636, RRID: AB_309864 Mouse monoclonal anti-DNA Polymerase iota (POLI, Clone OTI1H9) Abcam Cat# ab157244 Mouse monoclonal BV421-conjugated antiCD24 (Clone ML5) BD Biosciences Cat# 562789, RRID: AB_2737796 Mouse monoclonal Alexa Fluor 647-conjugated ani-EpCAM (Clone VU1D9) Cell Signaling Technology Cat# 5447, RRID: AB_10693458 Rabbit monoclonal anti-BRCA2 (Clone D9S6V) Cell Signaling Technology Cat# 10741, RRID: AB_2797730 Rabbit monoclonal anti-RAD52 (Clone EPR3464(2)) Abcam Cat# ab124971, RRID: AB_10971685 Mouse monoclonal anti-β-Actin (Clone 8H10D10) Cell Signaling Technology Cat# 3700, RRID: AB_2242334 Rabbit monoclonal anti-Vinculin (Clone E1E9V) Cell Signaling Technology Cat# 13901, RRID: AB_2728768 Mouse monoclonal anti-BrdU (Clone B44) BD Biosciences Cat# 347580, RRID: AB_400326 Rat monoclonal anti-BrdU (Clone BU1/75 (ICR1)) Abcam Cat# ab6326, RRID: AB_305426 Biological samples Human breast tissues from prophylactic mastectomies of BRCA2 mutation carriers Tissue Procurement Core, Cleveland Clinic N/A Chemicals, peptides, and recombinant proteins Aphidicolin Sigma-Aldrich Cat# 5047440001 Colcemid Gibco Cat# 15210 Potassium chloride Sigma Cat# P9327 Giemsa Sigma-Aldrich Cat# 32884 Cytochalasin-B Sigma Cat# C2743 CIdU (5-Chloro-2′-deoxyuridine) Sigma-Aldrich Cat# C6891 IdU (5-Iodo-2′-deoxyuridine) Sigma-Aldrich Cat# I7125 Lipofectamine RNAiMAX ThermoFisher Scientific Cat# 13778075 DAPI Invitrogen Cat# P36962 DMEM/F12 media ThermoFisher Scientific Cat# 11330057 Horse Serum ThermoFisher Scientific Cat# 16050122 Insulin Sigma Cat# I-1882 EGF Peprotech Cat# AF-100–15 Hydrocortisone Sigma Cat# H6909 (Continued on next page) 12 iScience 29, 117830, November 20, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Cholera toxin Sigma Aldrich Cat# C8052 Mammary Epithelial Cell Basal Medium ATCC Cat# PCS-600–030, Mammary Epithelial Cell Growth Kit ATCC Cat# PCS-600–040 DMEM/F-12 GlutaMAX ThermoFisher Scientific Cat# 10565018 L-glutamine ThermoFisher Scientific Cat# 25030081 Gentamycin/amphotericin-B ThermoFisher Scientific Cat# R01510 Critical commercial assays Click-iT EdU Alexa Fluor 488 flow cytometry assay kit Invitrogen Cat# C10425 Click-iT EdU Alexa Fluor 488 Imaging kit Invitrogen Cat# C10637 FxCycle Violet Ready Flow Reagent Invitrogen Cat# R37166 Live-or-Dye 510/551 Biotium Cat # 32012A MycoAlert Mycoplasma Detection Kit Lonza Cat# LT07–218 PierceTM Rapid Gold BCA Protein Assay Kit Thermo Scientific, Cat# A53225 RIPA buffer Cell Signaling Technology Cat# 9803/9806 Protease and phosphatase inhibitors Cell Signaling Technology Cat# 5872 PMSF Cell Signaling Technology Cat# 8553 SDS-PAGE Precast Protein Gels Bio-Rad Cat# 4561084 Clarity Max Western ECL Substrate Bio-Rad Cat# 1705062 Experimental models: Cell lines Human mammary epithelial cell line: MCF10A Cleveland Clinic Research, cell and media production core facility Cat# ATCC, CRL-10317 Primary Human Mammary Epithelial Cells: HMECs American Type Culture Collection (ATCC) Cat# ATCC, PCS-600–010 Primary BRCA2-mutant Human Mammary Epithelial Cells: BRCA2mut/- HMECs Tissue Procurement Core, Cleveland Clinic Caputo et al.55 Human mammary cancer cell line: MCF7 Cleveland Clinic Research, cell and media production core facility Cat# ATCC, HTB-22 Human cervical cancer cell line: HeLa Cleveland Clinic Research, cell and media production core facility Cat# ATCC, CRM-CCL-2 Oligonucleotides siRNA sequences Dharmacon Horizon Discovery Supplementary file, this paper Software and algorithms R (version 4.5.3) R Software https://www.r-project.org/ Seurat (version 5.4.0) Hao, Y. et al., Dictionary learning for integrative, multimodal and scalable singlecell analysis. ..

    Analysis:

    Article Title: Polymerase iota coordinates adaptation to replication stress in BRCA2-deficient mammary epithelial cells.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-FANCD2 (Clone EPR2302) Abcam Cat# ab108928, RRID: AB_10862535 Mouse monoclonal anti-phospho-Histone H3 Ser10 (Clone 6G3) Cell Signaling Technology Cat# 9706, RRID: AB_331748 Mouse monoclonal anti-Cyclin A2 (Clone 1C6C11) Proteintech Cat# 66391-1-Ig, RRID: AB_2881767 Rabbit polyclonal anti-53BP1 Cell Signaling Technology Cat# 4937, RRID: AB_10694558 Rat monoclonal anti-RPA32/RPA2 (Clone 4E4) Cell Signaling Technology Cat# 2208, RRID: AB_2238543 Mouse monoclonal anti-γH2AX Ser139 (Clone JBW301) Sigma-Aldrich (EMD Millipore) Cat# 05–636, RRID: AB_309864 Mouse monoclonal anti-DNA Polymerase iota (POLI, Clone OTI1H9) Abcam Cat# ab157244 Mouse monoclonal BV421-conjugated antiCD24 (Clone ML5) BD Biosciences Cat# 562789, RRID: AB_2737796 Mouse monoclonal Alexa Fluor 647-conjugated ani-EpCAM (Clone VU1D9) Cell Signaling Technology Cat# 5447, RRID: AB_10693458 Rabbit monoclonal anti-BRCA2 (Clone D9S6V) Cell Signaling Technology Cat# 10741, RRID: AB_2797730 Rabbit monoclonal anti-RAD52 (Clone EPR3464(2)) Abcam Cat# ab124971, RRID: AB_10971685 Mouse monoclonal anti-β-Actin (Clone 8H10D10) Cell Signaling Technology Cat# 3700, RRID: AB_2242334 Rabbit monoclonal anti-Vinculin (Clone E1E9V) Cell Signaling Technology Cat# 13901, RRID: AB_2728768 Mouse monoclonal anti-BrdU (Clone B44) BD Biosciences Cat# 347580, RRID: AB_400326 Rat monoclonal anti-BrdU (Clone BU1/75 (ICR1)) Abcam Cat# ab6326, RRID: AB_305426 Biological samples Human breast tissues from prophylactic mastectomies of BRCA2 mutation carriers Tissue Procurement Core, Cleveland Clinic N/A Chemicals, peptides, and recombinant proteins Aphidicolin Sigma-Aldrich Cat# 5047440001 Colcemid Gibco Cat# 15210 Potassium chloride Sigma Cat# P9327 Giemsa Sigma-Aldrich Cat# 32884 Cytochalasin-B Sigma Cat# C2743 CIdU (5-Chloro-2′-deoxyuridine) Sigma-Aldrich Cat# C6891 IdU (5-Iodo-2′-deoxyuridine) Sigma-Aldrich Cat# I7125 Lipofectamine RNAiMAX ThermoFisher Scientific Cat# 13778075 DAPI Invitrogen Cat# P36962 DMEM/F12 media ThermoFisher Scientific Cat# 11330057 Horse Serum ThermoFisher Scientific Cat# 16050122 Insulin Sigma Cat# I-1882 EGF Peprotech Cat# AF-100–15 Hydrocortisone Sigma Cat# H6909 (Continued on next page) 12 iScience 29, 117830, November 20, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Cholera toxin Sigma Aldrich Cat# C8052 Mammary Epithelial Cell Basal Medium ATCC Cat# PCS-600–030, Mammary Epithelial Cell Growth Kit ATCC Cat# PCS-600–040 DMEM/F-12 GlutaMAX ThermoFisher Scientific Cat# 10565018 L-glutamine ThermoFisher Scientific Cat# 25030081 Gentamycin/amphotericin-B ThermoFisher Scientific Cat# R01510 Critical commercial assays Click-iT EdU Alexa Fluor 488 flow cytometry assay kit Invitrogen Cat# C10425 Click-iT EdU Alexa Fluor 488 Imaging kit Invitrogen Cat# C10637 FxCycle Violet Ready Flow Reagent Invitrogen Cat# R37166 Live-or-Dye 510/551 Biotium Cat # 32012A MycoAlert Mycoplasma Detection Kit Lonza Cat# LT07–218 PierceTM Rapid Gold BCA Protein Assay Kit Thermo Scientific, Cat# A53225 RIPA buffer Cell Signaling Technology Cat# 9803/9806 Protease and phosphatase inhibitors Cell Signaling Technology Cat# 5872 PMSF Cell Signaling Technology Cat# 8553 SDS-PAGE Precast Protein Gels Bio-Rad Cat# 4561084 Clarity Max Western ECL Substrate Bio-Rad Cat# 1705062 Experimental models: Cell lines Human mammary epithelial cell line: MCF10A Cleveland Clinic Research, cell and media production core facility Cat# ATCC, CRL-10317 Primary Human Mammary Epithelial Cells: HMECs American Type Culture Collection (ATCC) Cat# ATCC, PCS-600–010 Primary BRCA2-mutant Human Mammary Epithelial Cells: BRCA2mut/- HMECs Tissue Procurement Core, Cleveland Clinic Caputo et al.55 Human mammary cancer cell line: MCF7 Cleveland Clinic Research, cell and media production core facility Cat# ATCC, HTB-22 Human cervical cancer cell line: HeLa Cleveland Clinic Research, cell and media production core facility Cat# ATCC, CRM-CCL-2 Oligonucleotides siRNA sequences Dharmacon Horizon Discovery Supplementary file, this paper Software and algorithms R (version 4.5.3) R Software https://www.r-project.org/ Seurat (version 5.4.0) Hao, Y. et al., Dictionary learning for integrative, multimodal and scalable singlecell analysis. ..



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