Journal: Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology

Article Title: The entomotoxin Jack Bean Urease changes cathepsin D activity in nymphs of the hematophagous insect Dipetalogaster maxima (Hemiptera: Reduviidae).

doi: 10.1016/j.cbpb.2020.110511

Figure Lengend Snippet: Fig. 1. DmCatD expression profile (mRNA and protein) in fifth instar nymphs of D. maxima. The anterior (AM) and posterior midgut (PM), the fat body (FB), the Hemocytes (He) and the hemolymph (Hemo) from fed nymphs were processed for qPCR (A) and Western blot (B), as described in Materials and Methods. For qPCR, total RNA was extracted and the DmCatD transcripts were quantified using specific primers and 18S ribosomal RNA as normalizer. ***P < 0.0001 vs fat body, hemocytes and posterior midgut (n = 5). For Western blot, approximately 50 μg of proteins were loaded in each lane, separated by 15% SDS-PAGE and probed with the anti-CatD antibody. Whole cell MCF7 lysate (0.2 μg) was employed as a positive control. Densitometric analyses of three independent blots were performed upon the intensity of the pro-DmCatD and DmCatD bands detected in each lane and the amount of total proteins loaded and stained with Ponceau S.

Article Snippet: Rabbit polyclonal anti-human cathepsin D antibody (sc-10725) and MCF7 whole cell lysates (Santa Cruz Biotechnology, Palo Alto, CA, USA); Alexa Fluor 568-conjugated goat anti-rabbit IgG antibody and 4′,6-diamidino-2-phenylindole (DAPI) (Molecular Probes, Carlsbad, CA, USA); Tissue-Tek embedding medium Optimal Cutting Temperature (OCT, Miles, Elkhart, IN, USA); Fluorsave (Calbiochem, Darmstadt, Germany); electrophoresis protein standards (New England Biolabs, Ipswich, MA, USA); MMLV reverse transcriptase (Promega, Heidelberg, Germany); PCR Primers (Sigma Genosys, Houston, TX, USA); MasterPure RNA Purification Kit (Epicenter Biotechnologies, Madison, WI, USA); Power SYBR® Green PCR Master Mix (Applied Biosystems, Foster City, CA, USA) were from indicated commercial sources.

Techniques: Expressing, Western Blot, SDS Page, Positive Control, Staining

Journal: Pharmaceuticals

Article Title: Multitargeting Pt(IV) Anticancer Prodrugs Bearing Mono- and Bis-Probenecid Ligands in Axial Positions: Synthesis and Evaluation of Biological Activity

doi: 10.3390/ph19030386

Figure Lengend Snippet: Cellular uptake of Pt in the whole cells or in the nuclei of MCF-7 cells after exposure to 20 μM SPP, DPP, or CDDP for 24 h. Results were shown as mean ± SD of three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: The Human breast cancer cell lines MCF-7 (CRL-3435), T47D (CRL-2865), and the triple-negative breast cancer cell line MDA-MB-231 (CRM-HTB-26) were obtained from the American Type Culture Collection (ATCC).

Techniques:

Journal: Pharmaceuticals

Article Title: Multitargeting Pt(IV) Anticancer Prodrugs Bearing Mono- and Bis-Probenecid Ligands in Axial Positions: Synthesis and Evaluation of Biological Activity

doi: 10.3390/ph19030386

Figure Lengend Snippet: ( A ) Representative apoptosis assay results of tested complexes on MCF-7 cells by flow cytometry. Q1 represents the percentage of necrotic cells, Q2 late apoptotic cells, Q3 early apoptotic cells, and Q4 living cells. ( B ) Average Population of various statuses of cells based on triple independent experiments. * p < 0.05, ** p < 0.01 and **** p < 0.0001.

Article Snippet: The Human breast cancer cell lines MCF-7 (CRL-3435), T47D (CRL-2865), and the triple-negative breast cancer cell line MDA-MB-231 (CRM-HTB-26) were obtained from the American Type Culture Collection (ATCC).

Techniques: Apoptosis Assay, Flow Cytometry

Journal: Pharmaceuticals

Article Title: Multitargeting Pt(IV) Anticancer Prodrugs Bearing Mono- and Bis-Probenecid Ligands in Axial Positions: Synthesis and Evaluation of Biological Activity

doi: 10.3390/ph19030386

Figure Lengend Snippet: Thermal shift binding assay in the absence ( A ) or presence ( B ) of 100 μM DPP in MCF-7 cells to evaluate the interaction between OAT1 and DPP.

Article Snippet: The Human breast cancer cell lines MCF-7 (CRL-3435), T47D (CRL-2865), and the triple-negative breast cancer cell line MDA-MB-231 (CRM-HTB-26) were obtained from the American Type Culture Collection (ATCC).

Techniques: Binding Assay

Journal: Pharmaceuticals

Article Title: Multitargeting Pt(IV) Anticancer Prodrugs Bearing Mono- and Bis-Probenecid Ligands in Axial Positions: Synthesis and Evaluation of Biological Activity

doi: 10.3390/ph19030386

Figure Lengend Snippet: Expression of OAT1 protein in MCF-7 cells in the presence of different concentrations of DPP.

Article Snippet: The Human breast cancer cell lines MCF-7 (CRL-3435), T47D (CRL-2865), and the triple-negative breast cancer cell line MDA-MB-231 (CRM-HTB-26) were obtained from the American Type Culture Collection (ATCC).

Techniques: Expressing