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fix-perm buffer  (Becton Dickinson)

 
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    Becton Dickinson fix-perm buffer
    Fix Perm Buffer, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/maxpar+barcode+permeabilization+buffer/fix+perm+buffer/pm34893771-246-7-9
    Average 90 stars, based on 1 article reviews
    fix-perm buffer - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    other:

    Article Title: Newborn and child-like molecular signatures in older adults stem from TCR shifts across human lifespan
    Article Snippet: Cells were fixed with BD Fix-Perm buffer (BD Biosciences) for 20 min, before intracellular staining for 30 min on ice with anti-TNF-α-APC (1:100 dilution, BD 340534), anti-granzyme B-AF700 (1:50 dilution, BD 560213), anti-IFN-γ-FITC (1:100 dilution, eBioscience 45-7-319-42) and perforin (1:10 dilution, BioLegend 353316) in perm wash buffer (BD Biosciences).

    Article Title: BCG Induces Protection against Mycobacterium tuberculosis Infection in the Wistar Rat Model
    Article Snippet: Subsequently, cells were stained with FITC-anti-CD8α mAb and PECy7-anti-CD4 mAb (all BD Pharmingen, Franklin Lakes, NJ), and, after 20 min on ice, cells were washed with PBS and fixed and permeablized for 20 min on ice using Fix-perm buffer (Ctyofix/Cytoperm kit, BD Pharmingen).

    Article Title: Ethanol-Induced Alterations of T Cells and Cytokines after Surgery in a Murine Infection Model
    Article Snippet: Afterwards, cells were stained and incubated with CD3 Pacific Blue (Pacific Blue Hamster Anti-Mouse CD3, BD Biosciences, 500A2), CD4 APC-H7 (APC-H7 Rat Anti-Mouse CD4, BD Biosciences, GK1.5), and CD8 PerCP (PerCP Rat Anti-Mouse CD8a, BD Biosciences, 53-6.7) before fixation with Perm-Fix-buffer (Cytofix/Cytoperm Solution for fixation and permeabilization, BD Biosciences) for 10 minutes.

    Article Title: Cell-Sized Lipid Vesicles as Artificial Antigen-Presenting Cells for Antigen-Specific T Cell Activation
    Article Snippet: The cells were then fixed and permeabilized by Fix-Perm buffer (BD Biosciences) and stained with Granzyme B.

    Article Title: Neurocognitive and hypokinetic movement disorder with features of parkinsonism after BCMA-targeting CAR-T cell therapy.
    Article Snippet: Barcoded cells were fixed and permeabilized with Fix-Perm buffer (BD Biosciences) and stained with the remaining intracellular antibodies from CyTOF Panel 1.

    Staining:

    Article Title: Multi-omic single-cell snapshots reveal multiple independent trajectories to drug tolerance in a melanoma cell line
    Article Snippet: .. All cells were then fixed with Fix-Perm buffer from BD Bioscience and then stained for intracellular dye-conjugated antibodies for MART1, NGFR, AXL, p-ERK, and Ki67. .. Flow cytometry analysis was conducted using Attune NxT Flow Cytometer from Thermo Fisher and the data were analyzed using FlowJo software.

    Article Title: Biobran/MGN-3, an Arabinoxylan Rice Bran, Exerts Anti-COVID-19 Effects and Boosts Immunity in Human Subjects
    Article Snippet: .. Cells were then washed, and CD8 antibody was used for surface staining for 30 min, following which cells were fixed, made permeable by fix perm buffer (BD Biosciences), and intracellularly stained with granzyme B and perforin. ..

    Incubation:

    Article Title: Antitumor immunity induced by antibody-based natural killer cell engager therapeutics armed with not-alpha IL-2 variant
    Article Snippet: .. After incubation, the cells were fixed immediately with FixPerm Buffer (BD) and incubated for 50 minutes on ice before permeabilization by incubation with Perm buffer III (BD) for 20 minutes at 4°C. .. Cells were then stained with anti-phospho-STAT5 (BD, 612598) and gating antibodies for 60 minutes at 4°C before flow cytometry analysis.



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    maxpar barcode permeabilization buffer  (fluidigm)
    95
    fluidigm maxpar barcode permeabilization buffer
    Activated CD4 + T cells bifurcate along the TCF1/BLIMP1 axis. (A) VAC063C single-cell RNAseq workflow: each sample of flow-sorted CD4 + T cells was barcoded using TotalSeq-C oligo-tagged antibodies; samples from all volunteers and time points were pooled (separately for first and third infection); and pooled samples were superloaded onto a 10X Chromium Controller (we aimed to capture 30,000 singlets per pool). GEMs encapsulating a single cell (or doublets) were then generated and from each GEM three libraries were produced: (1) the cell surface <t>barcode,</t> (2) 5′ gene expression, and (3) TCR (after amplification of the V(D)J regions). Libraries were pooled at the specified ratios and sequenced. Finally, we used PCA-based clustering to debarcode all samples and remove doublets (see Materials and methods). (B) Cell Ranger was used to align 5′ gene expression and V(D)J sequencing reads (independently for the first and third infection). Shown is the output of Cell Ranger after removing doublets and performing QC. (C–F) Data from all volunteers and time points was concatenated for UMAP analysis. The expression intensity of markers for memory (C), activation (D), and follicular helper T (T FH ) cell differentiation (E) are shown across the UMAP. The blue line represents the split between naive and memory cells whereas the green line represents the split between memory and activated cells. In F, the expression intensity of the master transcription factors associated with terminal differentiation (BLIMP1) versus the maintenance of stem-like properties (TCF1) are shown. In all cases, each UMAP is equivalent to those shown in (for cross-reference) and square brackets indicate that common protein names have been used. (G) Proposed model of T cell activation during a first-in-life malaria episode. The maintenance of stem-like T cells is essential for long-lived memory; this requires sustained expression of TCF1 to repress BLIMP1 and prevent the terminal differentiation of short-lived effector cells. In A–F, n = 3 for first and third infection.
    Maxpar Barcode Permeabilization Buffer, supplied by fluidigm, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/maxpar+barcode+permeabilization+buffer/Maxpar+Barcode+Perm+Buffer/pmc11987708-381-5-10
    Average 95 stars, based on 1 article reviews
    maxpar barcode permeabilization buffer - by Bioz Stars, 2026-11
    95/100 stars
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    Activated CD4 + T cells bifurcate along the TCF1/BLIMP1 axis. (A) VAC063C single-cell RNAseq workflow: each sample of flow-sorted CD4 + T cells was barcoded using TotalSeq-C oligo-tagged antibodies; samples from all volunteers and time points were pooled (separately for first and third infection); and pooled samples were superloaded onto a 10X Chromium Controller (we aimed to capture 30,000 singlets per pool). GEMs encapsulating a single cell (or doublets) were then generated and from each GEM three libraries were produced: (1) the cell surface barcode, (2) 5′ gene expression, and (3) TCR (after amplification of the V(D)J regions). Libraries were pooled at the specified ratios and sequenced. Finally, we used PCA-based clustering to debarcode all samples and remove doublets (see Materials and methods). (B) Cell Ranger was used to align 5′ gene expression and V(D)J sequencing reads (independently for the first and third infection). Shown is the output of Cell Ranger after removing doublets and performing QC. (C–F) Data from all volunteers and time points was concatenated for UMAP analysis. The expression intensity of markers for memory (C), activation (D), and follicular helper T (T FH ) cell differentiation (E) are shown across the UMAP. The blue line represents the split between naive and memory cells whereas the green line represents the split between memory and activated cells. In F, the expression intensity of the master transcription factors associated with terminal differentiation (BLIMP1) versus the maintenance of stem-like properties (TCF1) are shown. In all cases, each UMAP is equivalent to those shown in (for cross-reference) and square brackets indicate that common protein names have been used. (G) Proposed model of T cell activation during a first-in-life malaria episode. The maintenance of stem-like T cells is essential for long-lived memory; this requires sustained expression of TCF1 to repress BLIMP1 and prevent the terminal differentiation of short-lived effector cells. In A–F, n = 3 for first and third infection.

    Journal: The Journal of Experimental Medicine

    Article Title: Plasmodium falciparum infection induces T cell tolerance that is associated with decreased disease severity upon re-infection

    doi: 10.1084/jem.20241667

    Figure Lengend Snippet: Activated CD4 + T cells bifurcate along the TCF1/BLIMP1 axis. (A) VAC063C single-cell RNAseq workflow: each sample of flow-sorted CD4 + T cells was barcoded using TotalSeq-C oligo-tagged antibodies; samples from all volunteers and time points were pooled (separately for first and third infection); and pooled samples were superloaded onto a 10X Chromium Controller (we aimed to capture 30,000 singlets per pool). GEMs encapsulating a single cell (or doublets) were then generated and from each GEM three libraries were produced: (1) the cell surface barcode, (2) 5′ gene expression, and (3) TCR (after amplification of the V(D)J regions). Libraries were pooled at the specified ratios and sequenced. Finally, we used PCA-based clustering to debarcode all samples and remove doublets (see Materials and methods). (B) Cell Ranger was used to align 5′ gene expression and V(D)J sequencing reads (independently for the first and third infection). Shown is the output of Cell Ranger after removing doublets and performing QC. (C–F) Data from all volunteers and time points was concatenated for UMAP analysis. The expression intensity of markers for memory (C), activation (D), and follicular helper T (T FH ) cell differentiation (E) are shown across the UMAP. The blue line represents the split between naive and memory cells whereas the green line represents the split between memory and activated cells. In F, the expression intensity of the master transcription factors associated with terminal differentiation (BLIMP1) versus the maintenance of stem-like properties (TCF1) are shown. In all cases, each UMAP is equivalent to those shown in (for cross-reference) and square brackets indicate that common protein names have been used. (G) Proposed model of T cell activation during a first-in-life malaria episode. The maintenance of stem-like T cells is essential for long-lived memory; this requires sustained expression of TCF1 to repress BLIMP1 and prevent the terminal differentiation of short-lived effector cells. In A–F, n = 3 for first and third infection.

    Article Snippet: Next cells were permeabilized with Maxpar barcode permeabilization buffer (#201057; Fluidigm), and each sample was barcoded using Cell-ID 20-plex palladium barcodes (#201060; Fluidigm).

    Techniques: Infection, Generated, Produced, Gene Expression, Amplification, Sequencing, Expressing, Activation Assay, Cell Differentiation