maxima 5× rt buffer (Thermo Fisher)
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maxima 5× rt buffer
Maxima 5× Rt Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/maxima+rt+buffer/maxima+h+minus+reverse+transcriptase/pm40181168-187-5-16
Average 90 stars, based on 1 article reviews
Maxima 5× Rt Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/maxima+rt+buffer/maxima+h+minus+reverse+transcriptase/pm40181168-187-5-16
Average 90 stars, based on 1 article reviews
maxima 5× rt buffer - by Bioz Stars,
2026-10
90/100 stars
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Reverse Transcription:Article Title: Optogenetic Clustering of Human IRE1 Reveals Differential Regulation of Transcription and mRNA Splice Isoform Abundance by the UPR Article Snippet: .. The reaction was then quickly mixed with 0.5 μl RNaseOUT (Thermo Fisher #10777019), 1 μl of 20mM strand-switching primer, 1 μl Article Title: Deciphering direct transcriptional effects of epigenetic compounds through large-scale new RNA profiling Article Snippet: Briefly, 2 μl purified RNA from each sample was transferred to a 384 well plate and 2 μl alkylation mix (50 mM Tris-HCl pH 8.4, 45% DMSO (Sigma), 200 mM iodoacetamide (Sigma)) was added to each well and the plate was incubated at 50 °C for 15 min. To quench the alkylation reaction, 2 μl quenching mix (35 mM DTT (Thermo Scientific), 0.5 mM dNTPs (Thermo Scientific), 0.5 μM Smart-seq3 oligo-dT primer (5′-biotin-ACGAGCATCAGCAGCATACGA T30VN-3′; IDT), 0.4 U/μl RRI (Takara)) was added and the plate was incubated for 5 min at 21 °C, then 10 min at 72 °C to denature any RNA secondary structures. .. Reverse transcription master mix (25 mM Tris-HCl pH 8.0, 35 mM NaCl (Sigma), 1 mM GTP (Thermo Scientific), 3.6 mM MgCl 2 (invitrogen), 5% PEG (Sigma), 2 mM DTT (Thermo Scientific), 0,4 U/μl RNase Inhibitor (Takara), Smart-seq3 TSO oligo (5′-biotin-AGAGACAGATTGCGCAATGNNNNNNNNrGrGrG-3′; IDT), 2 U/μl Article Title: Nucleosome Positioning Shapes Cryptic Antisense Transcription Article Snippet: .. Reverse transcription was then performed using Article Title: The rewiring of cAMP/cGMP and LDH signalling drives cardiac hypertrophy in Pde5a -/- mice. Article Snippet: The yield and purity of RNA was determined with the NanoDrop OneC microvolume UV-Vis Spectrophotometer (Thermo Fisher Scientific). .. RNA was reverse transcribed by Article Title: Genetically encoded and modular subcellular organelle probes reveal dysfunction in lysosomes and mitochondria driven by PRKN knockout Article Snippet: RNA extraction kit , Zymo , Cat# R2062. .. Article Title: Genetically encoded and modular subcellular organelle probes reveal dysfunction in lysosomes and mitochondria driven by PRKN knockout Article Snippet: RNA was extracted from samples using TRIzol (Invitrogen 15596018) and an RNA extraction kit following the manufacturer’s recommended protocol (Zymo R2062). .. RNA concentration and purity were measured on a spectrophotometer. cDNA was produced from 1 μg of RNA per sample, using Article Title: Nanoscale 3D DNA tracing in non-denatured cells resolves the Cohesin-dependent loop architecture of the genome in situ Article Snippet: At 70-80% confluence cells were trypsinized with 0.05% Trypsin/EDTA (Cat.# 25300054, ThermoFisher) and transferred to a new culture dish at appropriate dilutions every 2–3 days. .. At this point, 1 μl of this sample was set-aside for quality control. ssDNA amplification from template RNA was performed by reverse transcription, using: 150 μl of unpurified in vitro transcription reaction, 1.7 mM dNTP mix, 19 μM forward primer, 240 U RNAsin Plus RNAse inhibitor (Promega), 1200 U Incubation:Article Title: Deciphering direct transcriptional effects of epigenetic compounds through large-scale new RNA profiling Article Snippet: Briefly, 2 μl purified RNA from each sample was transferred to a 384 well plate and 2 μl alkylation mix (50 mM Tris-HCl pH 8.4, 45% DMSO (Sigma), 200 mM iodoacetamide (Sigma)) was added to each well and the plate was incubated at 50 °C for 15 min. To quench the alkylation reaction, 2 μl quenching mix (35 mM DTT (Thermo Scientific), 0.5 mM dNTPs (Thermo Scientific), 0.5 μM Smart-seq3 oligo-dT primer (5′-biotin-ACGAGCATCAGCAGCATACGA T30VN-3′; IDT), 0.4 U/μl RRI (Takara)) was added and the plate was incubated for 5 min at 21 °C, then 10 min at 72 °C to denature any RNA secondary structures. .. Reverse transcription master mix (25 mM Tris-HCl pH 8.0, 35 mM NaCl (Sigma), 1 mM GTP (Thermo Scientific), 3.6 mM MgCl 2 (invitrogen), 5% PEG (Sigma), 2 mM DTT (Thermo Scientific), 0,4 U/μl RNase Inhibitor (Takara), Smart-seq3 TSO oligo (5′-biotin-AGAGACAGATTGCGCAATGNNNNNNNNrGrGrG-3′; IDT), 2 U/μl Polymerase Chain Reaction:Article Title: Nucleosome Positioning Shapes Cryptic Antisense Transcription Article Snippet: .. Reverse transcription was then performed using other:Article Title: Deciphering direct transcriptional effects of epigenetic compounds through large-scale new RNA profiling Article Snippet: Following the quenching reaction, the cells were denatured at 72 °C for 10 min and 3 μl RT reaction (2 μl TSO (5’-Biotin-AGAGACAGATTGCGCAATGNNNNNNNNrGrGrG-3’; IDT), 25 mM Tris-HCl (pH 8.0), 35 mM NaCl, 1 mM Real-time Polymerase Chain Reaction:Article Title: The rewiring of cAMP/cGMP and LDH signalling drives cardiac hypertrophy in Pde5a -/- mice. Article Snippet: The yield and purity of RNA was determined with the NanoDrop OneC microvolume UV-Vis Spectrophotometer (Thermo Fisher Scientific). .. RNA was reverse transcribed by Article Title: Genetically encoded and modular subcellular organelle probes reveal dysfunction in lysosomes and mitochondria driven by PRKN knockout Article Snippet: RNA was extracted from samples using TRIzol (Invitrogen 15596018) and an RNA extraction kit following the manufacturer’s recommended protocol (Zymo R2062). .. RNA concentration and purity were measured on a spectrophotometer. cDNA was produced from 1 μg of RNA per sample, using SYBR Green Assay:Article Title: The rewiring of cAMP/cGMP and LDH signalling drives cardiac hypertrophy in Pde5a -/- mice. Article Snippet: The yield and purity of RNA was determined with the NanoDrop OneC microvolume UV-Vis Spectrophotometer (Thermo Fisher Scientific). .. RNA was reverse transcribed by Article Title: Genetically encoded and modular subcellular organelle probes reveal dysfunction in lysosomes and mitochondria driven by PRKN knockout Article Snippet: RNA was extracted from samples using TRIzol (Invitrogen 15596018) and an RNA extraction kit following the manufacturer’s recommended protocol (Zymo R2062). .. RNA concentration and purity were measured on a spectrophotometer. cDNA was produced from 1 μg of RNA per sample, using Concentration Assay:Article Title: Genetically encoded and modular subcellular organelle probes reveal dysfunction in lysosomes and mitochondria driven by PRKN knockout Article Snippet: RNA was extracted from samples using TRIzol (Invitrogen 15596018) and an RNA extraction kit following the manufacturer’s recommended protocol (Zymo R2062). .. RNA concentration and purity were measured on a spectrophotometer. cDNA was produced from 1 μg of RNA per sample, using Spectrophotometry:Article Title: Genetically encoded and modular subcellular organelle probes reveal dysfunction in lysosomes and mitochondria driven by PRKN knockout Article Snippet: RNA was extracted from samples using TRIzol (Invitrogen 15596018) and an RNA extraction kit following the manufacturer’s recommended protocol (Zymo R2062). .. RNA concentration and purity were measured on a spectrophotometer. cDNA was produced from 1 μg of RNA per sample, using Produced:Article Title: Genetically encoded and modular subcellular organelle probes reveal dysfunction in lysosomes and mitochondria driven by PRKN knockout Article Snippet: RNA was extracted from samples using TRIzol (Invitrogen 15596018) and an RNA extraction kit following the manufacturer’s recommended protocol (Zymo R2062). .. RNA concentration and purity were measured on a spectrophotometer. cDNA was produced from 1 μg of RNA per sample, using Control:Article Title: Nanoscale 3D DNA tracing in non-denatured cells resolves the Cohesin-dependent loop architecture of the genome in situ Article Snippet: At 70-80% confluence cells were trypsinized with 0.05% Trypsin/EDTA (Cat.# 25300054, ThermoFisher) and transferred to a new culture dish at appropriate dilutions every 2–3 days. .. At this point, 1 μl of this sample was set-aside for quality control. ssDNA amplification from template RNA was performed by reverse transcription, using: 150 μl of unpurified in vitro transcription reaction, 1.7 mM dNTP mix, 19 μM forward primer, 240 U RNAsin Plus RNAse inhibitor (Promega), 1200 U Amplification:Article Title: Nanoscale 3D DNA tracing in non-denatured cells resolves the Cohesin-dependent loop architecture of the genome in situ Article Snippet: At 70-80% confluence cells were trypsinized with 0.05% Trypsin/EDTA (Cat.# 25300054, ThermoFisher) and transferred to a new culture dish at appropriate dilutions every 2–3 days. .. At this point, 1 μl of this sample was set-aside for quality control. ssDNA amplification from template RNA was performed by reverse transcription, using: 150 μl of unpurified in vitro transcription reaction, 1.7 mM dNTP mix, 19 μM forward primer, 240 U RNAsin Plus RNAse inhibitor (Promega), 1200 U In Vitro:Article Title: Nanoscale 3D DNA tracing in non-denatured cells resolves the Cohesin-dependent loop architecture of the genome in situ Article Snippet: At 70-80% confluence cells were trypsinized with 0.05% Trypsin/EDTA (Cat.# 25300054, ThermoFisher) and transferred to a new culture dish at appropriate dilutions every 2–3 days. .. At this point, 1 μl of this sample was set-aside for quality control. ssDNA amplification from template RNA was performed by reverse transcription, using: 150 μl of unpurified in vitro transcription reaction, 1.7 mM dNTP mix, 19 μM forward primer, 240 U RNAsin Plus RNAse inhibitor (Promega), 1200 U |