poly a mix (New England Biolabs)

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New England Biolabs poly a mix
Poly A Mix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1413 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/maxima+rt+buffer/E%2Ecoli+Poly+(A)+Polymerase/pm39843737-480-13-40
Average 99 stars, based on 1413 article reviews

poly a mix - by Bioz Stars, 2026-10

99/100 stars

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Purification:

Article Title: Ribonuclease κ promotes longevity by preventing age-associated accumulation of circular RNA in stress granules.
Article Snippet: Article Ribonuclease κ promotes longevity by preventing age-associated accumulation of circular RNA in stress granules

Article Title: Timing-Dependent Immunostimulatory Activity of Human IFN-β mRNA in Primary Human Myeloid Cells
Article Snippet: In vitro transcription with co-transcriptional capping was performed using the NEB HiScribe High Yield RNA Kit (New England Biolabs, Frankfurt a. M., Germany) and the TriLink CleanCap AG 3’OMe analog (TriLink BioTechnologies, San Diego, CA, USA), with complete substitution of uridine by N1-methylpseudouridine. .. Following silica-membrane purification (NEB Monarch RNA Cleanup Kit) the RNA was enzymatically tailed using an E. coli poly(A) polymerase (NEB), resulting in a poly(A) tail longer than 100 bases. .. The mRNA subsequently was purified by silica-membrane purification and sorting with oligo d(T) magnetic beads (Dynabeads mRNA Purification Kit; Thermo Fisher Scientific, Waltham, MA, USA) to reduce dsRNA contamination.

Article Title: Cotranslational membrane protein biogenesis by an EMC-bound translocon.
Article Snippet: After adding Nature Structural & Molecular Biology Article https://doi.org/10.1038/s41594-026-01889-2 100 μl of chloroform (Sigma-Aldrich, cat. no. C2432), RNA in the aqueous layer was separated by centrifugation at 12,000g for 15 min at 4 °C and precipitated overnight by adding 0.1 volume of 3 M sodium acetate (Invitrogen, cat. no. AM9740), 20 μg of GlycoBlue (Invitrogen, cat. no. AM9515) and 1.2 volumes of isopropanol. .. Purified RNase footprints used for DNA library preparation were resuspended in 5 μl of T4 polynucleotide kinase (PNK) reaction mixture (1× PNK buffer, 10 U of PNK (NEB, cat. no. M0201L) and 10 U of SUPERaseIn (Invitrogen, cat. no. AM2694)) and end-repaired at 37 °C for 1 h. Next, 5 μl of polyadenylation mix containing 10 U of Escherichia coli poly (A) polymerase (NEB, cat. no. M0276L), 2 μl of 5× first-strand buffer (250 mM Tris-HCl pH 8.3, 375 mM KCl, 15 mM MgCl2), 0.25 mM ATP and 10 U SUPERaseIn was added, and the reaction was incubated at 37 °C for 2 h. The resulting polyadenylated RNAs were reverse transcribed by SMART-RT, by adding 2.5 μl of reverse transcription primer (10 μM, 5′-ACGTGTGCTCTTCCGATCTNNNNNN NNNNNNNNNTTTTTTTTTTTTTTTVN-3′, where ‘N’ is any base and ‘V’ is ‘A’, ‘C’ or ‘G’), annealing by heating to 72 °C for 5 min and cooling on ice, and then supplementing with 17.6 μl of SMART-RT mixture (2.5 μl of dNTP (10 mM), 400 U of Superscript II reverse transcriptase (Invitrogen, cat. no. 18064014), 10 U of SUPERaseIn, 4 μl of Superscript II first-strand buffer (5×), 1.5 μl of DTT (100 mM), 6 μl of Betaine (5 M), 0.12 μl of MgCl2 (1 M), 0.5 μl of template-switching oligos (100 μM 5′-CTCTTTCCCTACACGACGCTCTTCCGATCTNNNNrGrG+G-3′, where ‘N’ is any base and ‘+G’ is a locked nucleic acid guanylate) and 0.5 μl of nuclease-free water). ..

De-Phosphorylation Assay:

Phospho-proteomics:

Generated:

Article Title: A Novel SINE Transposable Element-Associated lncRNA Regulates Zygotic Genome Activation in Porcine Parthenogenetically Activated Embryos
Article Snippet: For in vitro transcription of mRNAs, target gene fragments containing the T7 promoter sequence were amplified by PCR and transcribed using the HiScribe T7 High Yield RNA Synthesis Kit (E2040S, NEB, Ipswich, MA, USA). .. Capped mRNAs were generated by co-transcriptional addition of Cap1 analog (NEB, S1404) and polyadenylated with E. coli Poly(A) Polymerase (NEB, M0276) at 37 °C for 30 min to add ~150–200 nt poly(A) tails. ..

Incubation:

Article Title: Strained Partnership: Biocompatibility and Transcriptomic Responses of Hydrogenotrophic Methanogens to Methyl Diethanolamine in Bio-integrated Carbon Capture and Utilization
Article Snippet: .. Approximately 6 μg of total RNA was incubated at 37 °C for 30 min with 1 mM ATP and 5 U of E. coli poly (A) polymerase (NEB, England). ..

cDNA Synthesis:

Article Title: Circulating miRNAs (135a-5p, 646 AND 1271-5P) in Breast Cancer Diagnosis and Staging: Integrative Network and Functional Analysis.
Article Snippet: Synthetic cel-miR-39-3p (Qiagen, Germany) was added at 25 fmol to each plasma sample during RNA extraction as an external spike-in control to monitor RNA extraction efficiency, and RNA concentration and purity were assessed using a NanoDrop spectrophotometer as detailed previously (20-21). .. cDNA Synthesis and Quantitative RT-PCR A total of 100 ng RNA was polyadenylated using E. coli Poly(A) Polymerase (New England Biolabs, UK) and reverse transcribed using the GoScript Reverse Transcription Kit (Promega, USA). .. Quantitative RT-PCR was performed on a StepOnePlus Real-Time PCR System (Applied Biosystems, USA) using GoTaq qPCR Master Mix (Promega, USA) with miRNA-specific forward primers and a universal reverse primer (Supplementary Tables, Table S1).

Quantitative RT-PCR:

Reverse Transcription:

Isolation:

Article Title: Amplification-free colorimetric detection of exosomal miRNA using a CRISPR/Cas13a-activated HRP/Au/MoS 2 nanobiohybrid catalyst.
Article Snippet: .. Briefly, 2 μL of isolated miRNA was mixed with 2 μL of 10 mM ATP, 2 μL of E. coli poly(A) polymerase reaction buffer, 1 μL of E. coli poly(A) polymerase (NEB), and 13 μL of DEPC-treated water. ..

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