maxima rt buffer (New England Biolabs)

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New England Biolabs maxima rt buffer
Maxima Rt Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 7073 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/maxima+rt+buffer/Klenow+Fragment/pm36008377-275-15-24
Average 99 stars, based on 7073 article reviews

maxima rt buffer - by Bioz Stars, 2026-10

99/100 stars

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Labeling:

Article Title: 3D Chromatin Architecture Provides Insights Into Leaf Trait Variation Among Pear Species.
Article Snippet: Subsequently, the cross-linked nuclei were permeated with 0.3% SDS at 65◦C for 10 min. SDS were quenched by 1.8% Triton X100 at 37◦C for 15 min. Chromatin was then digested with 100 U of MboI (NEB) restriction enzyme for 2 h at 37◦C. .. DNA ends were labeled with biotin-14-dCTP (Invitrogen) by incubating with DNA Polymerase I, Large Klenow Fragment (NEB) at 37◦C for 45 min, after which the reaction was inactivated by incubation at 65◦C for 20 min. DNA ligation was performed by 50U T4 DNA ligase (Thermo) at 16◦C for 4 h. Afterward, proteinase K was added to the sample and incubated at 65◦C overnight to reverse the cross-linking. .. DNA was extracted using the Qiagen DNeasy Plant Mini Kit (Qiagen, #69106), following the manufacturer’s instructions.

Incubation:

Article Title: Enriched methylomes of low-input and fragmented DNA using fragment ligation EXclusive methylation sequencing
Article Snippet: This was followed by restriction enzyme cutting using 10 units of MspI (R0106T, NEB), followed by a 30-min incubation. .. Next, an end repair was performed using 1 μL of Klenow master mix composed of 1.3 μL of Klenow (M0212M, NEB), 2 μL of 10x NEB buffer 2, 5 μL of a master mix of NTPs, and 11.7 μL of water at an incubation of 30°C for 20 min, followed by 37°C for 20 min, and 65°C for 20 min. .. The mix of NTPs was composed of 40 μL of the 10 mM dATP, 4 μL of the 10 mM dCTP, and 4 μL of the 10 mM dGTP solution (N0446S, NEB).

DNA Ligation:

Hi-C:

Article Title: Lamins and lineage-relevant transcription factors coordinate gene expression in lineage development
Article Snippet: .. A-tailing of Hi-C DNA was performed by adding 2.5 μL of 10X NEB2 buffer (B7002S), 5 μL of 1 mM dATP (N0440S), and 1.5 μL of Klenow fragment (NEB, M0212L) to the 16 μL of resuspended streptavidin beads. ..

Purification:

Article Title: Serum Response Factor (SRF) promotes actin cytoskeletal organization in adipocytes to support adaptive hypertrophic expansion and tissue remodeling during obesity in mice
Article Snippet: .. Briefly, purified DNA was subject to end repair and phosphorylation using the End-It DNA End-Repair Kit (Epicenter, ER0720), followed by A-tailing with Klenow Fragment (NEB, M0212) and adaptor ligation with Quick Ligase (NEB, M2200). .. Libraries were then PCR-amplified for 18 cycles with PfuUltra II Hotstart PCR Master Mix (Agilent, 600850).

Article Title: Influenza A virus NS1 sequesters RNA:DNA hybrids to evade RNase H1-dependent innate immunity
Article Snippet: .. Second-strand synthesis was performed on ssDNA using random hexamers, dNTPs, and the Large (Klenow) Fragment (NEB) according to the manufacturer’s instructions, followed by purification with 2x volume AMPure XP beads (Beckman Coulter). .. Sequencing libraries were prepared from the resulting dsDNA using the Nextera XT DNA Library Preparation Kit and sequenced on the MiSeq platform (Illumina, to a depth of ≥1 5 single-end 151-bp reads).

Phospho-proteomics:

Ligation:

Concentration Assay:

Article Title: HM-DyadCap – capture and mapping of 5-hydroxymethylcytosine/5-methylcytosine CpG dyads in mammalian DNA
Article Snippet: .. Adapters were annealed at a final concentration of 2.5 μM in the same way (o4371 + o4372, o4373 + o4374, and o4392 + o4393) and subsequently extended with 25 mU/μl Klenow fragment (NEB) in the presence of 0.1 mM dNTPs for 20 min at 37°C. ..

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