anti-halotag antibody promega cat#g9281 (Promega)
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Anti Halotag Antibody Promega Cat#G9281, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/matlab+trapz+function/anti+halotag/pm27716509-219-2-8
Average 90 stars, based on 1 article reviews
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Incubation:Article Title: Visualizing PIEZO1 localization and activity in hiPSC-derived single cells and organoids with HaloTag technology. Article Snippet: Article Title: Supplementary Information for HaloTag as a Substrate-Based Macroautophagy Reporter Article Snippet: Article Title: Conformational plasticity of a BiP-GRP94 chaperone complex. Article Snippet: 6-Chlorohexanol (Sigma-Aldrich, C45008), trypsin (Sigma-Aldrich, T8003), DSBU (Thermo Fisher, A35459), glutaraldehyde (Thermo Fisher), ATP (Sigma-Aldrich, A6419), AMP-PNP (Sigma-Aldrich, A2647), ADP (ARCOS Organics, 164670010), PU-WS13 (MedChemExpress, HY-18680), HALTS1 (N-(3,5-dichloro-2-ethoxybenzyl)-2H-tetrazol5-amine)71 (ChemBridge Corporation, 9074451), cycloheximide (Sigma-Aldrich, 01810), doxycycline hyclate (Sigma-Aldrich, DD9891), anti-His5 (Santa Cruz Biotechnologies, SC-8036), anti-Strep (Qiagen, 34850), anti-BiP C50B12 (Cell Signaling, 3177), anti-GRP94 (Proteintech, 14700-1-AP), anti-HA tag C29F4 (Cell Signaling 3724), Article Title: Transient inhibition of cell division in competent pneumococcal cells results from deceleration of the septal peptidoglycan complex. Article Snippet: Article Title: TopBP1 coordinates DNA repair synthesis in mitosis via recruitment of the nuclease scaffold SLX4. Article Snippet: Primary antibodies used: Mouse anti-GFP (1:1000, Roche, 11814460001), Article Title: The reaction mechanism for glycolysis side product degradation by Parkinson’s disease–linked DJ-1 Article Snippet: Proteins subjected to SDS-PAGE were transferred to polyvinylidene difluoride membranes and blocked with 1% (wt/vol) skim-milk/TBS-T for 30 min. To detect the indicated proteins, mouse anti–DJ-1 3E8 (ADI-KAM-SA100E; at 1:2,000 dilution; RRID:AB_2039445; ENZO), mouse anti-GAPDH (Cat #MAB374; 1:200; RRID:AB_2107445; Millipore), mouse anti-methylglyoxal (Cat #STA-011; 1:1,000; RRID:AB_3096122; Cell Biolabs), mouse anti-Halo (Cat #G9211; 1:1,000; RRID:AB_2688011; Promega), and mouse anti–Beta-actin (Cat #M177-3; 1:4,000; RRID:AB_10697039; MBL) antibodies were used as primary antibodies and incubated for 2 h. Article Title: Immune cells regulate circulating adipocyte extracellular vesicle levels in response to metabolic shifts Article Snippet: After another 3x 10 minutes washes in ultrapure water, the samples were blocked in 10% normal goat serum (NGS), washed 3x 10 minutes in PBS, then incubated in primary antibody (anti-Halo; Article Title: Article Snippet: Blots were rinsed briefly in 5 mL PBST, then incubated for 1 h with 300 gentle rocking in PBST with either Gentle:Article Title: Visualizing PIEZO1 localization and activity in hiPSC-derived single cells and organoids with HaloTag technology. Article Snippet: Article Title: Supplementary Information for HaloTag as a Substrate-Based Macroautophagy Reporter Article Snippet: Article Title: Conformational plasticity of a BiP-GRP94 chaperone complex. Article Snippet: 6-Chlorohexanol (Sigma-Aldrich, C45008), trypsin (Sigma-Aldrich, T8003), DSBU (Thermo Fisher, A35459), glutaraldehyde (Thermo Fisher), ATP (Sigma-Aldrich, A6419), AMP-PNP (Sigma-Aldrich, A2647), ADP (ARCOS Organics, 164670010), PU-WS13 (MedChemExpress, HY-18680), HALTS1 (N-(3,5-dichloro-2-ethoxybenzyl)-2H-tetrazol5-amine)71 (ChemBridge Corporation, 9074451), cycloheximide (Sigma-Aldrich, 01810), doxycycline hyclate (Sigma-Aldrich, DD9891), anti-His5 (Santa Cruz Biotechnologies, SC-8036), anti-Strep (Qiagen, 34850), anti-BiP C50B12 (Cell Signaling, 3177), anti-GRP94 (Proteintech, 14700-1-AP), anti-HA tag C29F4 (Cell Signaling 3724), Article Title: Transient inhibition of cell division in competent pneumococcal cells results from deceleration of the septal peptidoglycan complex. Article Snippet: Article Title: TopBP1 coordinates DNA repair synthesis in mitosis via recruitment of the nuclease scaffold SLX4. Article Snippet: Primary antibodies used: Mouse anti-GFP (1:1000, Roche, 11814460001), Article Title: The reaction mechanism for glycolysis side product degradation by Parkinson’s disease–linked DJ-1 Article Snippet: Proteins subjected to SDS-PAGE were transferred to polyvinylidene difluoride membranes and blocked with 1% (wt/vol) skim-milk/TBS-T for 30 min. To detect the indicated proteins, mouse anti–DJ-1 3E8 (ADI-KAM-SA100E; at 1:2,000 dilution; RRID:AB_2039445; ENZO), mouse anti-GAPDH (Cat #MAB374; 1:200; RRID:AB_2107445; Millipore), mouse anti-methylglyoxal (Cat #STA-011; 1:1,000; RRID:AB_3096122; Cell Biolabs), mouse anti-Halo (Cat #G9211; 1:1,000; RRID:AB_2688011; Promega), and mouse anti–Beta-actin (Cat #M177-3; 1:4,000; RRID:AB_10697039; MBL) antibodies were used as primary antibodies and incubated for 2 h. Article Title: Immune cells regulate circulating adipocyte extracellular vesicle levels in response to metabolic shifts Article Snippet: After another 3x 10 minutes washes in ultrapure water, the samples were blocked in 10% normal goat serum (NGS), washed 3x 10 minutes in PBS, then incubated in primary antibody (anti-Halo; Article Title: Article Snippet: Blots were rinsed briefly in 5 mL PBST, then incubated for 1 h with 300 gentle rocking in PBST with either SDS Page:Article Title: Visualizing PIEZO1 localization and activity in hiPSC-derived single cells and organoids with HaloTag technology. Article Snippet: Article Title: Supplementary Information for HaloTag as a Substrate-Based Macroautophagy Reporter Article Snippet: Article Title: Conformational plasticity of a BiP-GRP94 chaperone complex. Article Snippet: 6-Chlorohexanol (Sigma-Aldrich, C45008), trypsin (Sigma-Aldrich, T8003), DSBU (Thermo Fisher, A35459), glutaraldehyde (Thermo Fisher), ATP (Sigma-Aldrich, A6419), AMP-PNP (Sigma-Aldrich, A2647), ADP (ARCOS Organics, 164670010), PU-WS13 (MedChemExpress, HY-18680), HALTS1 (N-(3,5-dichloro-2-ethoxybenzyl)-2H-tetrazol5-amine)71 (ChemBridge Corporation, 9074451), cycloheximide (Sigma-Aldrich, 01810), doxycycline hyclate (Sigma-Aldrich, DD9891), anti-His5 (Santa Cruz Biotechnologies, SC-8036), anti-Strep (Qiagen, 34850), anti-BiP C50B12 (Cell Signaling, 3177), anti-GRP94 (Proteintech, 14700-1-AP), anti-HA tag C29F4 (Cell Signaling 3724), Article Title: Transient inhibition of cell division in competent pneumococcal cells results from deceleration of the septal peptidoglycan complex. Article Snippet: Article Title: TopBP1 coordinates DNA repair synthesis in mitosis via recruitment of the nuclease scaffold SLX4. Article Snippet: Primary antibodies used: Mouse anti-GFP (1:1000, Roche, 11814460001), Article Title: The reaction mechanism for glycolysis side product degradation by Parkinson’s disease–linked DJ-1 Article Snippet: Proteins subjected to SDS-PAGE were transferred to polyvinylidene difluoride membranes and blocked with 1% (wt/vol) skim-milk/TBS-T for 30 min. To detect the indicated proteins, mouse anti–DJ-1 3E8 (ADI-KAM-SA100E; at 1:2,000 dilution; RRID:AB_2039445; ENZO), mouse anti-GAPDH (Cat #MAB374; 1:200; RRID:AB_2107445; Millipore), mouse anti-methylglyoxal (Cat #STA-011; 1:1,000; RRID:AB_3096122; Cell Biolabs), mouse anti-Halo (Cat #G9211; 1:1,000; RRID:AB_2688011; Promega), and mouse anti–Beta-actin (Cat #M177-3; 1:4,000; RRID:AB_10697039; MBL) antibodies were used as primary antibodies and incubated for 2 h. Article Title: Immune cells regulate circulating adipocyte extracellular vesicle levels in response to metabolic shifts Article Snippet: After another 3x 10 minutes washes in ultrapure water, the samples were blocked in 10% normal goat serum (NGS), washed 3x 10 minutes in PBS, then incubated in primary antibody (anti-Halo; Article Title: Article Snippet: Blots were rinsed briefly in 5 mL PBST, then incubated for 1 h with 300 gentle rocking in PBST with either Western Blot:Article Title: Visualizing PIEZO1 localization and activity in hiPSC-derived single cells and organoids with HaloTag technology. Article Snippet: Article Title: Supplementary Information for HaloTag as a Substrate-Based Macroautophagy Reporter Article Snippet: Article Title: Conformational plasticity of a BiP-GRP94 chaperone complex. Article Snippet: 6-Chlorohexanol (Sigma-Aldrich, C45008), trypsin (Sigma-Aldrich, T8003), DSBU (Thermo Fisher, A35459), glutaraldehyde (Thermo Fisher), ATP (Sigma-Aldrich, A6419), AMP-PNP (Sigma-Aldrich, A2647), ADP (ARCOS Organics, 164670010), PU-WS13 (MedChemExpress, HY-18680), HALTS1 (N-(3,5-dichloro-2-ethoxybenzyl)-2H-tetrazol5-amine)71 (ChemBridge Corporation, 9074451), cycloheximide (Sigma-Aldrich, 01810), doxycycline hyclate (Sigma-Aldrich, DD9891), anti-His5 (Santa Cruz Biotechnologies, SC-8036), anti-Strep (Qiagen, 34850), anti-BiP C50B12 (Cell Signaling, 3177), anti-GRP94 (Proteintech, 14700-1-AP), anti-HA tag C29F4 (Cell Signaling 3724), Article Title: Transient inhibition of cell division in competent pneumococcal cells results from deceleration of the septal peptidoglycan complex. Article Snippet: Article Title: TopBP1 coordinates DNA repair synthesis in mitosis via recruitment of the nuclease scaffold SLX4. Article Snippet: Primary antibodies used: Mouse anti-GFP (1:1000, Roche, 11814460001), Article Title: The reaction mechanism for glycolysis side product degradation by Parkinson’s disease–linked DJ-1 Article Snippet: Proteins subjected to SDS-PAGE were transferred to polyvinylidene difluoride membranes and blocked with 1% (wt/vol) skim-milk/TBS-T for 30 min. To detect the indicated proteins, mouse anti–DJ-1 3E8 (ADI-KAM-SA100E; at 1:2,000 dilution; RRID:AB_2039445; ENZO), mouse anti-GAPDH (Cat #MAB374; 1:200; RRID:AB_2107445; Millipore), mouse anti-methylglyoxal (Cat #STA-011; 1:1,000; RRID:AB_3096122; Cell Biolabs), mouse anti-Halo (Cat #G9211; 1:1,000; RRID:AB_2688011; Promega), and mouse anti–Beta-actin (Cat #M177-3; 1:4,000; RRID:AB_10697039; MBL) antibodies were used as primary antibodies and incubated for 2 h. Article Title: Immune cells regulate circulating adipocyte extracellular vesicle levels in response to metabolic shifts Article Snippet: After another 3x 10 minutes washes in ultrapure water, the samples were blocked in 10% normal goat serum (NGS), washed 3x 10 minutes in PBS, then incubated in primary antibody (anti-Halo; Article Title: Article Snippet: Blots were rinsed briefly in 5 mL PBST, then incubated for 1 h with 300 gentle rocking in PBST with either Next-Generation Sequencing:Article Title: Visualizing PIEZO1 localization and activity in hiPSC-derived single cells and organoids with HaloTag technology. Article Snippet: Article Title: Supplementary Information for HaloTag as a Substrate-Based Macroautophagy Reporter Article Snippet: Article Title: Conformational plasticity of a BiP-GRP94 chaperone complex. Article Snippet: 6-Chlorohexanol (Sigma-Aldrich, C45008), trypsin (Sigma-Aldrich, T8003), DSBU (Thermo Fisher, A35459), glutaraldehyde (Thermo Fisher), ATP (Sigma-Aldrich, A6419), AMP-PNP (Sigma-Aldrich, A2647), ADP (ARCOS Organics, 164670010), PU-WS13 (MedChemExpress, HY-18680), HALTS1 (N-(3,5-dichloro-2-ethoxybenzyl)-2H-tetrazol5-amine)71 (ChemBridge Corporation, 9074451), cycloheximide (Sigma-Aldrich, 01810), doxycycline hyclate (Sigma-Aldrich, DD9891), anti-His5 (Santa Cruz Biotechnologies, SC-8036), anti-Strep (Qiagen, 34850), anti-BiP C50B12 (Cell Signaling, 3177), anti-GRP94 (Proteintech, 14700-1-AP), anti-HA tag C29F4 (Cell Signaling 3724), Article Title: Transient inhibition of cell division in competent pneumococcal cells results from deceleration of the septal peptidoglycan complex. Article Snippet: Article Title: TopBP1 coordinates DNA repair synthesis in mitosis via recruitment of the nuclease scaffold SLX4. Article Snippet: Primary antibodies used: Mouse anti-GFP (1:1000, Roche, 11814460001), Article Title: The reaction mechanism for glycolysis side product degradation by Parkinson’s disease–linked DJ-1 Article Snippet: Proteins subjected to SDS-PAGE were transferred to polyvinylidene difluoride membranes and blocked with 1% (wt/vol) skim-milk/TBS-T for 30 min. To detect the indicated proteins, mouse anti–DJ-1 3E8 (ADI-KAM-SA100E; at 1:2,000 dilution; RRID:AB_2039445; ENZO), mouse anti-GAPDH (Cat #MAB374; 1:200; RRID:AB_2107445; Millipore), mouse anti-methylglyoxal (Cat #STA-011; 1:1,000; RRID:AB_3096122; Cell Biolabs), mouse anti-Halo (Cat #G9211; 1:1,000; RRID:AB_2688011; Promega), and mouse anti–Beta-actin (Cat #M177-3; 1:4,000; RRID:AB_10697039; MBL) antibodies were used as primary antibodies and incubated for 2 h. Article Title: Immune cells regulate circulating adipocyte extracellular vesicle levels in response to metabolic shifts Article Snippet: After another 3x 10 minutes washes in ultrapure water, the samples were blocked in 10% normal goat serum (NGS), washed 3x 10 minutes in PBS, then incubated in primary antibody (anti-Halo; Article Title: Article Snippet: Blots were rinsed briefly in 5 mL PBST, then incubated for 1 h with 300 gentle rocking in PBST with either |
