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Promega anti-halotag antibody promega cat#g9281
Anti Halotag Antibody Promega Cat#G9281, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/matlab+trapz+function/anti+halotag/pm27716509-219-2-8
Average 90 stars, based on 1 article reviews
anti-halotag antibody promega cat#g9281 - by Bioz Stars, 2026-09
90/100 stars

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Incubation:

Article Title: Visualizing PIEZO1 localization and activity in hiPSC-derived single cells and organoids with HaloTag technology.
Article Snippet: Western blotting was performed with 1:1000mouse anti-HaloTag (Promega, Cat. No. G9211) or 1:1000mouse anti-PIEZO1 (Novus Biologicals, Cat. No. NBP2-75617), followed by 1:1000 Goat anti-Mouse IgG-Secondary antibody, HRP (Invitrogen, Cat No. 32430) or 1:100,000 HRP-mouse anti-actin (ThermoFisher Cat. No. 5-15739-HRP) in TBS, 0.1% Tween20 in 5% NonFat Dried Milk (Carnation).

Article Title: Supplementary Information for HaloTag as a Substrate-Based Macroautophagy Reporter
Article Snippet: AntiHaloTag monoclonal antibody (Cat. #G9211) was purchased from Promega.

Article Title: Conformational plasticity of a BiP-GRP94 chaperone complex.
Article Snippet: 6-Chlorohexanol (Sigma-Aldrich, C45008), trypsin (Sigma-Aldrich, T8003), DSBU (Thermo Fisher, A35459), glutaraldehyde (Thermo Fisher), ATP (Sigma-Aldrich, A6419), AMP-PNP (Sigma-Aldrich, A2647), ADP (ARCOS Organics, 164670010), PU-WS13 (MedChemExpress, HY-18680), HALTS1 (N-(3,5-dichloro-2-ethoxybenzyl)-2H-tetrazol5-amine)71 (ChemBridge Corporation, 9074451), cycloheximide (Sigma-Aldrich, 01810), doxycycline hyclate (Sigma-Aldrich, DD9891), anti-His5 (Santa Cruz Biotechnologies, SC-8036), anti-Strep (Qiagen, 34850), anti-BiP C50B12 (Cell Signaling, 3177), anti-GRP94 (Proteintech, 14700-1-AP), anti-HA tag C29F4 (Cell Signaling 3724), anti-HT (Promega, G9211), goat anti-mouse HRP-coupled secondary antibody (Invitrogen, 31444) and goat anti-rabbit HRP-coupled secondary antibody (Invitrogen, 31460) were from commercial suppliers.

Article Title: Transient inhibition of cell division in competent pneumococcal cells results from deceleration of the septal peptidoglycan complex.
Article Snippet: Proteins were then transferred to a nitrocellulosemembrane, using a Turbo Blot transfer unit (Bio-Rad). mNeonGreen, and HaloTag and anti ALFA-Tag fusion proteinsweredetected using polyclonal antimNeonGreen (NC, unpublished), and anti-HaloTag (G921A, Promega) and anti-ALFA (N1581, NanoTag Biotechnologies) antibodies diluted at 1:10,000, 1:5,000 and 1:10,000 respectively.

Article Title: TopBP1 coordinates DNA repair synthesis in mitosis via recruitment of the nuclease scaffold SLX4.
Article Snippet: Primary antibodies used: Mouse anti-GFP (1:1000, Roche, 11814460001), Rabbit anti-Halo (1:500, Promega, G9281), Sheep anti-SLX4 (1:100, S714C, gift from John Rouse, University of Dundee), Rabbit anti-FANCD2 (1:500, Novus, NB100182SS).

Article Title: The reaction mechanism for glycolysis side product degradation by Parkinson’s disease–linked DJ-1
Article Snippet: Proteins subjected to SDS-PAGE were transferred to polyvinylidene difluoride membranes and blocked with 1% (wt/vol) skim-milk/TBS-T for 30 min. To detect the indicated proteins, mouse anti–DJ-1 3E8 (ADI-KAM-SA100E; at 1:2,000 dilution; RRID:AB_2039445; ENZO), mouse anti-GAPDH (Cat #MAB374; 1:200; RRID:AB_2107445; Millipore), mouse anti-methylglyoxal (Cat #STA-011; 1:1,000; RRID:AB_3096122; Cell Biolabs), mouse anti-Halo (Cat #G9211; 1:1,000; RRID:AB_2688011; Promega), and mouse anti–Beta-actin (Cat #M177-3; 1:4,000; RRID:AB_10697039; MBL) antibodies were used as primary antibodies and incubated for 2 h. HRP-conjugated goat anti-mouse IgG (Cat #W4021; 1:10,000; RRID:AB_430834; Promega) was used as a secondary antibody and incubated for 45 min. Proteins were detected using a Western Lighting Plus-ECL Kit on a FUSION SOLO S system (VILBER).

Article Title: Immune cells regulate circulating adipocyte extracellular vesicle levels in response to metabolic shifts
Article Snippet: After another 3x 10 minutes washes in ultrapure water, the samples were blocked in 10% normal goat serum (NGS), washed 3x 10 minutes in PBS, then incubated in primary antibody (anti-Halo; Promega G9281) diluted 1:500 with 1% bovine serum albumin (BSA) in PBS for 1 hour at room temperature, followed by 2 days at 4°C.

Article Title:
Article Snippet: Blots were rinsed briefly in 5 mL PBST, then incubated for 1 h with 300 gentle rocking in PBST with either mouse anti-HT (1:600 dilution; Promega, G9211), 301 rabbit anti-MreC (1:1,400 dilution; (19-21)), or rabbit anti-bPBP2b (1:10,000 dilution; (18)).

Gentle:

Article Title: Visualizing PIEZO1 localization and activity in hiPSC-derived single cells and organoids with HaloTag technology.
Article Snippet: Western blotting was performed with 1:1000mouse anti-HaloTag (Promega, Cat. No. G9211) or 1:1000mouse anti-PIEZO1 (Novus Biologicals, Cat. No. NBP2-75617), followed by 1:1000 Goat anti-Mouse IgG-Secondary antibody, HRP (Invitrogen, Cat No. 32430) or 1:100,000 HRP-mouse anti-actin (ThermoFisher Cat. No. 5-15739-HRP) in TBS, 0.1% Tween20 in 5% NonFat Dried Milk (Carnation).

Article Title: Supplementary Information for HaloTag as a Substrate-Based Macroautophagy Reporter
Article Snippet: AntiHaloTag monoclonal antibody (Cat. #G9211) was purchased from Promega.

Article Title: Conformational plasticity of a BiP-GRP94 chaperone complex.
Article Snippet: 6-Chlorohexanol (Sigma-Aldrich, C45008), trypsin (Sigma-Aldrich, T8003), DSBU (Thermo Fisher, A35459), glutaraldehyde (Thermo Fisher), ATP (Sigma-Aldrich, A6419), AMP-PNP (Sigma-Aldrich, A2647), ADP (ARCOS Organics, 164670010), PU-WS13 (MedChemExpress, HY-18680), HALTS1 (N-(3,5-dichloro-2-ethoxybenzyl)-2H-tetrazol5-amine)71 (ChemBridge Corporation, 9074451), cycloheximide (Sigma-Aldrich, 01810), doxycycline hyclate (Sigma-Aldrich, DD9891), anti-His5 (Santa Cruz Biotechnologies, SC-8036), anti-Strep (Qiagen, 34850), anti-BiP C50B12 (Cell Signaling, 3177), anti-GRP94 (Proteintech, 14700-1-AP), anti-HA tag C29F4 (Cell Signaling 3724), anti-HT (Promega, G9211), goat anti-mouse HRP-coupled secondary antibody (Invitrogen, 31444) and goat anti-rabbit HRP-coupled secondary antibody (Invitrogen, 31460) were from commercial suppliers.

Article Title: Transient inhibition of cell division in competent pneumococcal cells results from deceleration of the septal peptidoglycan complex.
Article Snippet: Proteins were then transferred to a nitrocellulosemembrane, using a Turbo Blot transfer unit (Bio-Rad). mNeonGreen, and HaloTag and anti ALFA-Tag fusion proteinsweredetected using polyclonal antimNeonGreen (NC, unpublished), and anti-HaloTag (G921A, Promega) and anti-ALFA (N1581, NanoTag Biotechnologies) antibodies diluted at 1:10,000, 1:5,000 and 1:10,000 respectively.

Article Title: TopBP1 coordinates DNA repair synthesis in mitosis via recruitment of the nuclease scaffold SLX4.
Article Snippet: Primary antibodies used: Mouse anti-GFP (1:1000, Roche, 11814460001), Rabbit anti-Halo (1:500, Promega, G9281), Sheep anti-SLX4 (1:100, S714C, gift from John Rouse, University of Dundee), Rabbit anti-FANCD2 (1:500, Novus, NB100182SS).

Article Title: The reaction mechanism for glycolysis side product degradation by Parkinson’s disease–linked DJ-1
Article Snippet: Proteins subjected to SDS-PAGE were transferred to polyvinylidene difluoride membranes and blocked with 1% (wt/vol) skim-milk/TBS-T for 30 min. To detect the indicated proteins, mouse anti–DJ-1 3E8 (ADI-KAM-SA100E; at 1:2,000 dilution; RRID:AB_2039445; ENZO), mouse anti-GAPDH (Cat #MAB374; 1:200; RRID:AB_2107445; Millipore), mouse anti-methylglyoxal (Cat #STA-011; 1:1,000; RRID:AB_3096122; Cell Biolabs), mouse anti-Halo (Cat #G9211; 1:1,000; RRID:AB_2688011; Promega), and mouse anti–Beta-actin (Cat #M177-3; 1:4,000; RRID:AB_10697039; MBL) antibodies were used as primary antibodies and incubated for 2 h. HRP-conjugated goat anti-mouse IgG (Cat #W4021; 1:10,000; RRID:AB_430834; Promega) was used as a secondary antibody and incubated for 45 min. Proteins were detected using a Western Lighting Plus-ECL Kit on a FUSION SOLO S system (VILBER).

Article Title: Immune cells regulate circulating adipocyte extracellular vesicle levels in response to metabolic shifts
Article Snippet: After another 3x 10 minutes washes in ultrapure water, the samples were blocked in 10% normal goat serum (NGS), washed 3x 10 minutes in PBS, then incubated in primary antibody (anti-Halo; Promega G9281) diluted 1:500 with 1% bovine serum albumin (BSA) in PBS for 1 hour at room temperature, followed by 2 days at 4°C.

Article Title:
Article Snippet: Blots were rinsed briefly in 5 mL PBST, then incubated for 1 h with 300 gentle rocking in PBST with either mouse anti-HT (1:600 dilution; Promega, G9211), 301 rabbit anti-MreC (1:1,400 dilution; (19-21)), or rabbit anti-bPBP2b (1:10,000 dilution; (18)).

SDS Page:

Article Title: Visualizing PIEZO1 localization and activity in hiPSC-derived single cells and organoids with HaloTag technology.
Article Snippet: Western blotting was performed with 1:1000mouse anti-HaloTag (Promega, Cat. No. G9211) or 1:1000mouse anti-PIEZO1 (Novus Biologicals, Cat. No. NBP2-75617), followed by 1:1000 Goat anti-Mouse IgG-Secondary antibody, HRP (Invitrogen, Cat No. 32430) or 1:100,000 HRP-mouse anti-actin (ThermoFisher Cat. No. 5-15739-HRP) in TBS, 0.1% Tween20 in 5% NonFat Dried Milk (Carnation).

Article Title: Supplementary Information for HaloTag as a Substrate-Based Macroautophagy Reporter
Article Snippet: AntiHaloTag monoclonal antibody (Cat. #G9211) was purchased from Promega.

Article Title: Conformational plasticity of a BiP-GRP94 chaperone complex.
Article Snippet: 6-Chlorohexanol (Sigma-Aldrich, C45008), trypsin (Sigma-Aldrich, T8003), DSBU (Thermo Fisher, A35459), glutaraldehyde (Thermo Fisher), ATP (Sigma-Aldrich, A6419), AMP-PNP (Sigma-Aldrich, A2647), ADP (ARCOS Organics, 164670010), PU-WS13 (MedChemExpress, HY-18680), HALTS1 (N-(3,5-dichloro-2-ethoxybenzyl)-2H-tetrazol5-amine)71 (ChemBridge Corporation, 9074451), cycloheximide (Sigma-Aldrich, 01810), doxycycline hyclate (Sigma-Aldrich, DD9891), anti-His5 (Santa Cruz Biotechnologies, SC-8036), anti-Strep (Qiagen, 34850), anti-BiP C50B12 (Cell Signaling, 3177), anti-GRP94 (Proteintech, 14700-1-AP), anti-HA tag C29F4 (Cell Signaling 3724), anti-HT (Promega, G9211), goat anti-mouse HRP-coupled secondary antibody (Invitrogen, 31444) and goat anti-rabbit HRP-coupled secondary antibody (Invitrogen, 31460) were from commercial suppliers.

Article Title: Transient inhibition of cell division in competent pneumococcal cells results from deceleration of the septal peptidoglycan complex.
Article Snippet: Proteins were then transferred to a nitrocellulosemembrane, using a Turbo Blot transfer unit (Bio-Rad). mNeonGreen, and HaloTag and anti ALFA-Tag fusion proteinsweredetected using polyclonal antimNeonGreen (NC, unpublished), and anti-HaloTag (G921A, Promega) and anti-ALFA (N1581, NanoTag Biotechnologies) antibodies diluted at 1:10,000, 1:5,000 and 1:10,000 respectively.

Article Title: TopBP1 coordinates DNA repair synthesis in mitosis via recruitment of the nuclease scaffold SLX4.
Article Snippet: Primary antibodies used: Mouse anti-GFP (1:1000, Roche, 11814460001), Rabbit anti-Halo (1:500, Promega, G9281), Sheep anti-SLX4 (1:100, S714C, gift from John Rouse, University of Dundee), Rabbit anti-FANCD2 (1:500, Novus, NB100182SS).

Article Title: The reaction mechanism for glycolysis side product degradation by Parkinson’s disease–linked DJ-1
Article Snippet: Proteins subjected to SDS-PAGE were transferred to polyvinylidene difluoride membranes and blocked with 1% (wt/vol) skim-milk/TBS-T for 30 min. To detect the indicated proteins, mouse anti–DJ-1 3E8 (ADI-KAM-SA100E; at 1:2,000 dilution; RRID:AB_2039445; ENZO), mouse anti-GAPDH (Cat #MAB374; 1:200; RRID:AB_2107445; Millipore), mouse anti-methylglyoxal (Cat #STA-011; 1:1,000; RRID:AB_3096122; Cell Biolabs), mouse anti-Halo (Cat #G9211; 1:1,000; RRID:AB_2688011; Promega), and mouse anti–Beta-actin (Cat #M177-3; 1:4,000; RRID:AB_10697039; MBL) antibodies were used as primary antibodies and incubated for 2 h. HRP-conjugated goat anti-mouse IgG (Cat #W4021; 1:10,000; RRID:AB_430834; Promega) was used as a secondary antibody and incubated for 45 min. Proteins were detected using a Western Lighting Plus-ECL Kit on a FUSION SOLO S system (VILBER).

Article Title: Immune cells regulate circulating adipocyte extracellular vesicle levels in response to metabolic shifts
Article Snippet: After another 3x 10 minutes washes in ultrapure water, the samples were blocked in 10% normal goat serum (NGS), washed 3x 10 minutes in PBS, then incubated in primary antibody (anti-Halo; Promega G9281) diluted 1:500 with 1% bovine serum albumin (BSA) in PBS for 1 hour at room temperature, followed by 2 days at 4°C.

Article Title:
Article Snippet: Blots were rinsed briefly in 5 mL PBST, then incubated for 1 h with 300 gentle rocking in PBST with either mouse anti-HT (1:600 dilution; Promega, G9211), 301 rabbit anti-MreC (1:1,400 dilution; (19-21)), or rabbit anti-bPBP2b (1:10,000 dilution; (18)).

Western Blot:

Article Title: Visualizing PIEZO1 localization and activity in hiPSC-derived single cells and organoids with HaloTag technology.
Article Snippet: Western blotting was performed with 1:1000mouse anti-HaloTag (Promega, Cat. No. G9211) or 1:1000mouse anti-PIEZO1 (Novus Biologicals, Cat. No. NBP2-75617), followed by 1:1000 Goat anti-Mouse IgG-Secondary antibody, HRP (Invitrogen, Cat No. 32430) or 1:100,000 HRP-mouse anti-actin (ThermoFisher Cat. No. 5-15739-HRP) in TBS, 0.1% Tween20 in 5% NonFat Dried Milk (Carnation).

Article Title: Supplementary Information for HaloTag as a Substrate-Based Macroautophagy Reporter
Article Snippet: AntiHaloTag monoclonal antibody (Cat. #G9211) was purchased from Promega.

Article Title: Conformational plasticity of a BiP-GRP94 chaperone complex.
Article Snippet: 6-Chlorohexanol (Sigma-Aldrich, C45008), trypsin (Sigma-Aldrich, T8003), DSBU (Thermo Fisher, A35459), glutaraldehyde (Thermo Fisher), ATP (Sigma-Aldrich, A6419), AMP-PNP (Sigma-Aldrich, A2647), ADP (ARCOS Organics, 164670010), PU-WS13 (MedChemExpress, HY-18680), HALTS1 (N-(3,5-dichloro-2-ethoxybenzyl)-2H-tetrazol5-amine)71 (ChemBridge Corporation, 9074451), cycloheximide (Sigma-Aldrich, 01810), doxycycline hyclate (Sigma-Aldrich, DD9891), anti-His5 (Santa Cruz Biotechnologies, SC-8036), anti-Strep (Qiagen, 34850), anti-BiP C50B12 (Cell Signaling, 3177), anti-GRP94 (Proteintech, 14700-1-AP), anti-HA tag C29F4 (Cell Signaling 3724), anti-HT (Promega, G9211), goat anti-mouse HRP-coupled secondary antibody (Invitrogen, 31444) and goat anti-rabbit HRP-coupled secondary antibody (Invitrogen, 31460) were from commercial suppliers.

Article Title: Transient inhibition of cell division in competent pneumococcal cells results from deceleration of the septal peptidoglycan complex.
Article Snippet: Proteins were then transferred to a nitrocellulosemembrane, using a Turbo Blot transfer unit (Bio-Rad). mNeonGreen, and HaloTag and anti ALFA-Tag fusion proteinsweredetected using polyclonal antimNeonGreen (NC, unpublished), and anti-HaloTag (G921A, Promega) and anti-ALFA (N1581, NanoTag Biotechnologies) antibodies diluted at 1:10,000, 1:5,000 and 1:10,000 respectively.

Article Title: TopBP1 coordinates DNA repair synthesis in mitosis via recruitment of the nuclease scaffold SLX4.
Article Snippet: Primary antibodies used: Mouse anti-GFP (1:1000, Roche, 11814460001), Rabbit anti-Halo (1:500, Promega, G9281), Sheep anti-SLX4 (1:100, S714C, gift from John Rouse, University of Dundee), Rabbit anti-FANCD2 (1:500, Novus, NB100182SS).

Article Title: The reaction mechanism for glycolysis side product degradation by Parkinson’s disease–linked DJ-1
Article Snippet: Proteins subjected to SDS-PAGE were transferred to polyvinylidene difluoride membranes and blocked with 1% (wt/vol) skim-milk/TBS-T for 30 min. To detect the indicated proteins, mouse anti–DJ-1 3E8 (ADI-KAM-SA100E; at 1:2,000 dilution; RRID:AB_2039445; ENZO), mouse anti-GAPDH (Cat #MAB374; 1:200; RRID:AB_2107445; Millipore), mouse anti-methylglyoxal (Cat #STA-011; 1:1,000; RRID:AB_3096122; Cell Biolabs), mouse anti-Halo (Cat #G9211; 1:1,000; RRID:AB_2688011; Promega), and mouse anti–Beta-actin (Cat #M177-3; 1:4,000; RRID:AB_10697039; MBL) antibodies were used as primary antibodies and incubated for 2 h. HRP-conjugated goat anti-mouse IgG (Cat #W4021; 1:10,000; RRID:AB_430834; Promega) was used as a secondary antibody and incubated for 45 min. Proteins were detected using a Western Lighting Plus-ECL Kit on a FUSION SOLO S system (VILBER).

Article Title: Immune cells regulate circulating adipocyte extracellular vesicle levels in response to metabolic shifts
Article Snippet: After another 3x 10 minutes washes in ultrapure water, the samples were blocked in 10% normal goat serum (NGS), washed 3x 10 minutes in PBS, then incubated in primary antibody (anti-Halo; Promega G9281) diluted 1:500 with 1% bovine serum albumin (BSA) in PBS for 1 hour at room temperature, followed by 2 days at 4°C.

Article Title:
Article Snippet: Blots were rinsed briefly in 5 mL PBST, then incubated for 1 h with 300 gentle rocking in PBST with either mouse anti-HT (1:600 dilution; Promega, G9211), 301 rabbit anti-MreC (1:1,400 dilution; (19-21)), or rabbit anti-bPBP2b (1:10,000 dilution; (18)).

Next-Generation Sequencing:

Article Title: Visualizing PIEZO1 localization and activity in hiPSC-derived single cells and organoids with HaloTag technology.
Article Snippet: Western blotting was performed with 1:1000mouse anti-HaloTag (Promega, Cat. No. G9211) or 1:1000mouse anti-PIEZO1 (Novus Biologicals, Cat. No. NBP2-75617), followed by 1:1000 Goat anti-Mouse IgG-Secondary antibody, HRP (Invitrogen, Cat No. 32430) or 1:100,000 HRP-mouse anti-actin (ThermoFisher Cat. No. 5-15739-HRP) in TBS, 0.1% Tween20 in 5% NonFat Dried Milk (Carnation).

Article Title: Supplementary Information for HaloTag as a Substrate-Based Macroautophagy Reporter
Article Snippet: AntiHaloTag monoclonal antibody (Cat. #G9211) was purchased from Promega.

Article Title: Conformational plasticity of a BiP-GRP94 chaperone complex.
Article Snippet: 6-Chlorohexanol (Sigma-Aldrich, C45008), trypsin (Sigma-Aldrich, T8003), DSBU (Thermo Fisher, A35459), glutaraldehyde (Thermo Fisher), ATP (Sigma-Aldrich, A6419), AMP-PNP (Sigma-Aldrich, A2647), ADP (ARCOS Organics, 164670010), PU-WS13 (MedChemExpress, HY-18680), HALTS1 (N-(3,5-dichloro-2-ethoxybenzyl)-2H-tetrazol5-amine)71 (ChemBridge Corporation, 9074451), cycloheximide (Sigma-Aldrich, 01810), doxycycline hyclate (Sigma-Aldrich, DD9891), anti-His5 (Santa Cruz Biotechnologies, SC-8036), anti-Strep (Qiagen, 34850), anti-BiP C50B12 (Cell Signaling, 3177), anti-GRP94 (Proteintech, 14700-1-AP), anti-HA tag C29F4 (Cell Signaling 3724), anti-HT (Promega, G9211), goat anti-mouse HRP-coupled secondary antibody (Invitrogen, 31444) and goat anti-rabbit HRP-coupled secondary antibody (Invitrogen, 31460) were from commercial suppliers.

Article Title: Transient inhibition of cell division in competent pneumococcal cells results from deceleration of the septal peptidoglycan complex.
Article Snippet: Proteins were then transferred to a nitrocellulosemembrane, using a Turbo Blot transfer unit (Bio-Rad). mNeonGreen, and HaloTag and anti ALFA-Tag fusion proteinsweredetected using polyclonal antimNeonGreen (NC, unpublished), and anti-HaloTag (G921A, Promega) and anti-ALFA (N1581, NanoTag Biotechnologies) antibodies diluted at 1:10,000, 1:5,000 and 1:10,000 respectively.

Article Title: TopBP1 coordinates DNA repair synthesis in mitosis via recruitment of the nuclease scaffold SLX4.
Article Snippet: Primary antibodies used: Mouse anti-GFP (1:1000, Roche, 11814460001), Rabbit anti-Halo (1:500, Promega, G9281), Sheep anti-SLX4 (1:100, S714C, gift from John Rouse, University of Dundee), Rabbit anti-FANCD2 (1:500, Novus, NB100182SS).

Article Title: The reaction mechanism for glycolysis side product degradation by Parkinson’s disease–linked DJ-1
Article Snippet: Proteins subjected to SDS-PAGE were transferred to polyvinylidene difluoride membranes and blocked with 1% (wt/vol) skim-milk/TBS-T for 30 min. To detect the indicated proteins, mouse anti–DJ-1 3E8 (ADI-KAM-SA100E; at 1:2,000 dilution; RRID:AB_2039445; ENZO), mouse anti-GAPDH (Cat #MAB374; 1:200; RRID:AB_2107445; Millipore), mouse anti-methylglyoxal (Cat #STA-011; 1:1,000; RRID:AB_3096122; Cell Biolabs), mouse anti-Halo (Cat #G9211; 1:1,000; RRID:AB_2688011; Promega), and mouse anti–Beta-actin (Cat #M177-3; 1:4,000; RRID:AB_10697039; MBL) antibodies were used as primary antibodies and incubated for 2 h. HRP-conjugated goat anti-mouse IgG (Cat #W4021; 1:10,000; RRID:AB_430834; Promega) was used as a secondary antibody and incubated for 45 min. Proteins were detected using a Western Lighting Plus-ECL Kit on a FUSION SOLO S system (VILBER).

Article Title: Immune cells regulate circulating adipocyte extracellular vesicle levels in response to metabolic shifts
Article Snippet: After another 3x 10 minutes washes in ultrapure water, the samples were blocked in 10% normal goat serum (NGS), washed 3x 10 minutes in PBS, then incubated in primary antibody (anti-Halo; Promega G9281) diluted 1:500 with 1% bovine serum albumin (BSA) in PBS for 1 hour at room temperature, followed by 2 days at 4°C.

Article Title:
Article Snippet: Blots were rinsed briefly in 5 mL PBST, then incubated for 1 h with 300 gentle rocking in PBST with either mouse anti-HT (1:600 dilution; Promega, G9211), 301 rabbit anti-MreC (1:1,400 dilution; (19-21)), or rabbit anti-bPBP2b (1:10,000 dilution; (18)).



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Rectified soleus electromyography (EMG) traces (mean of 30 repetitions) from a control participant illustrating the heteronymous excitation (blue area) and inhibition (red area) from femoral nerve (FN) stimulation (2 × motor threshold, MT) onto ongoing soleus (SOL) EMG. Onset and offset latencies for excitation and inhibition are marked with blue and red circles, respectively. The solid horizontal black line represents the mean SOL background EMG, while the dashed horizontal line indicates one standard deviation below the mean. FN stimulation involved a single square pulse of 1 ms duration, resulting in a stimulation artifact (dashed vertical line). Excitation and inhibition areas (depicted as shaded blue and red regions, respectively) were quantified <t>using</t> <t>MATLAB’s</t> <t>‘trapz’</t> function, referenced to the mean background EMG. The duration was determined from the onset and offset timepoints
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Image Search Results


Rectified soleus electromyography (EMG) traces (mean of 30 repetitions) from a control participant illustrating the heteronymous excitation (blue area) and inhibition (red area) from femoral nerve (FN) stimulation (2 × motor threshold, MT) onto ongoing soleus (SOL) EMG. Onset and offset latencies for excitation and inhibition are marked with blue and red circles, respectively. The solid horizontal black line represents the mean SOL background EMG, while the dashed horizontal line indicates one standard deviation below the mean. FN stimulation involved a single square pulse of 1 ms duration, resulting in a stimulation artifact (dashed vertical line). Excitation and inhibition areas (depicted as shaded blue and red regions, respectively) were quantified using MATLAB’s ‘trapz’ function, referenced to the mean background EMG. The duration was determined from the onset and offset timepoints

Journal: Journal of NeuroEngineering and Rehabilitation

Article Title: Heteronymous feedback from quadriceps onto soleus in stroke survivors

doi: 10.1186/s12984-025-01572-5

Figure Lengend Snippet: Rectified soleus electromyography (EMG) traces (mean of 30 repetitions) from a control participant illustrating the heteronymous excitation (blue area) and inhibition (red area) from femoral nerve (FN) stimulation (2 × motor threshold, MT) onto ongoing soleus (SOL) EMG. Onset and offset latencies for excitation and inhibition are marked with blue and red circles, respectively. The solid horizontal black line represents the mean SOL background EMG, while the dashed horizontal line indicates one standard deviation below the mean. FN stimulation involved a single square pulse of 1 ms duration, resulting in a stimulation artifact (dashed vertical line). Excitation and inhibition areas (depicted as shaded blue and red regions, respectively) were quantified using MATLAB’s ‘trapz’ function, referenced to the mean background EMG. The duration was determined from the onset and offset timepoints

Article Snippet: Excitation and inhibition areas (depicted as shaded blue and red regions, respectively) were quantified using MATLAB’s ‘trapz’ function, referenced to the mean background EMG.

Techniques: Control, Inhibition, Standard Deviation