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    agena bioscience all-mentions
    All Mentions, supplied by agena bioscience, used in various techniques. Bioz Stars score: 99/100, based on 2840 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/massarray+quantitative+methylation+analysis+platform/all-mentions/custom%40all-mentions%4038528048
    Average 99 stars, based on 2840 article reviews
    all-mentions - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    Multiplex Assay:

    Article Title: Design and validation of an SNP-based assay for detecting Juglans ailantifolia introgression into Juglans cinerea populations or living collections
    Article Snippet: lignment of the original CAPS primers with the chloroplast genomes of J. cinerea, J. ailantifolia, J. nigra, J. regia, and Juglans mandshurica L. (Dong et al. 2017). Genotyping was performed using the Agena Bioscience iPLEX Gold platform at the Genome Quebec service facility (Montreal, Canada). In short, a multiplex PCR was performed on 20 ng of template genomic DNA in a 5uL reaction mixture contai

    Polymerase Chain Reaction:

    Article Title: Design and validation of an SNP-based assay for detecting Juglans ailantifolia introgression into Juglans cinerea populations or living collections
    Article Snippet: lignment of the original CAPS primers with the chloroplast genomes of J. cinerea, J. ailantifolia, J. nigra, J. regia, and Juglans mandshurica L. (Dong et al. 2017). Genotyping was performed using the Agena Bioscience iPLEX Gold platform at the Genome Quebec service facility (Montreal, Canada). In short, a multiplex PCR was performed on 20 ng of template genomic DNA in a 5uL reaction mixture contai

    Multiplex PCR:

    Article Title: Design and validation of an SNP-based assay for detecting Juglans ailantifolia introgression into Juglans cinerea populations or living collections
    Article Snippet: lignment of the original CAPS primers with the chloroplast genomes of J. cinerea, J. ailantifolia, J. nigra, J. regia, and Juglans mandshurica L. (Dong et al. 2017). Genotyping was performed using the Agena Bioscience iPLEX Gold platform at the Genome Quebec service facility (Montreal, Canada). In short, a multiplex PCR was performed on 20 ng of template genomic DNA in a 5uL reaction mixture contai

    Genotyping Assay:

    Article Title: Design and validation of an SNP-based assay for detecting Juglans ailantifolia introgression into Juglans cinerea populations or living collections
    Article Snippet: lignment of the original CAPS primers with the chloroplast genomes of J. cinerea, J. ailantifolia, J. nigra, J. regia, and Juglans mandshurica L. (Dong et al. 2017). Genotyping was performed using the Agena Bioscience iPLEX Gold platform at the Genome Quebec service facility (Montreal, Canada). In short, a multiplex PCR was performed on 20 ng of template genomic DNA in a 5uL reaction mixture contai

    Purification:

    Article Title: Design and validation of an SNP-based assay for detecting Juglans ailantifolia introgression into Juglans cinerea populations or living collections
    Article Snippet: lignment of the original CAPS primers with the chloroplast genomes of J. cinerea, J. ailantifolia, J. nigra, J. regia, and Juglans mandshurica L. (Dong et al. 2017). Genotyping was performed using the Agena Bioscience iPLEX Gold platform at the Genome Quebec service facility (Montreal, Canada). In short, a multiplex PCR was performed on 20 ng of template genomic DNA in a 5uL reaction mixture contai

    DNA Purification:

    Article Title: Design and validation of an SNP-based assay for detecting Juglans ailantifolia introgression into Juglans cinerea populations or living collections
    Article Snippet: lignment of the original CAPS primers with the chloroplast genomes of J. cinerea, J. ailantifolia, J. nigra, J. regia, and Juglans mandshurica L. (Dong et al. 2017). Genotyping was performed using the Agena Bioscience iPLEX Gold platform at the Genome Quebec service facility (Montreal, Canada). In short, a multiplex PCR was performed on 20 ng of template genomic DNA in a 5uL reaction mixture contai

    Hot Start PCR:

    Article Title: Design and validation of an SNP-based assay for detecting Juglans ailantifolia introgression into Juglans cinerea populations or living collections
    Article Snippet: lignment of the original CAPS primers with the chloroplast genomes of J. cinerea, J. ailantifolia, J. nigra, J. regia, and Juglans mandshurica L. (Dong et al. 2017). Genotyping was performed using the Agena Bioscience iPLEX Gold platform at the Genome Quebec service facility (Montreal, Canada). In short, a multiplex PCR was performed on 20 ng of template genomic DNA in a 5uL reaction mixture contai

    Mutagenesis:

    Article Title: Design and validation of an SNP-based assay for detecting Juglans ailantifolia introgression into Juglans cinerea populations or living collections
    Article Snippet: lignment of the original CAPS primers with the chloroplast genomes of J. cinerea, J. ailantifolia, J. nigra, J. regia, and Juglans mandshurica L. (Dong et al. 2017). Genotyping was performed using the Agena Bioscience iPLEX Gold platform at the Genome Quebec service facility (Montreal, Canada). In short, a multiplex PCR was performed on 20 ng of template genomic DNA in a 5uL reaction mixture contai

    Generated:

    Article Title: Design and validation of an SNP-based assay for detecting Juglans ailantifolia introgression into Juglans cinerea populations or living collections
    Article Snippet: lignment of the original CAPS primers with the chloroplast genomes of J. cinerea, J. ailantifolia, J. nigra, J. regia, and Juglans mandshurica L. (Dong et al. 2017). Genotyping was performed using the Agena Bioscience iPLEX Gold platform at the Genome Quebec service facility (Montreal, Canada). In short, a multiplex PCR was performed on 20 ng of template genomic DNA in a 5uL reaction mixture contai

    Methylation:

    Article Title: Design and validation of an SNP-based assay for detecting Juglans ailantifolia introgression into Juglans cinerea populations or living collections
    Article Snippet: lignment of the original CAPS primers with the chloroplast genomes of J. cinerea, J. ailantifolia, J. nigra, J. regia, and Juglans mandshurica L. (Dong et al. 2017). Genotyping was performed using the Agena Bioscience iPLEX Gold platform at the Genome Quebec service facility (Montreal, Canada). In short, a multiplex PCR was performed on 20 ng of template genomic DNA in a 5uL reaction mixture contai

    DNA Methylation Assay:

    Article Title: Design and validation of an SNP-based assay for detecting Juglans ailantifolia introgression into Juglans cinerea populations or living collections
    Article Snippet: lignment of the original CAPS primers with the chloroplast genomes of J. cinerea, J. ailantifolia, J. nigra, J. regia, and Juglans mandshurica L. (Dong et al. 2017). Genotyping was performed using the Agena Bioscience iPLEX Gold platform at the Genome Quebec service facility (Montreal, Canada). In short, a multiplex PCR was performed on 20 ng of template genomic DNA in a 5uL reaction mixture contai

    Mass Spectrometry:

    Article Title: Design and validation of an SNP-based assay for detecting Juglans ailantifolia introgression into Juglans cinerea populations or living collections
    Article Snippet: lignment of the original CAPS primers with the chloroplast genomes of J. cinerea, J. ailantifolia, J. nigra, J. regia, and Juglans mandshurica L. (Dong et al. 2017). Genotyping was performed using the Agena Bioscience iPLEX Gold platform at the Genome Quebec service facility (Montreal, Canada). In short, a multiplex PCR was performed on 20 ng of template genomic DNA in a 5uL reaction mixture contai

    Amplification:

    Article Title: Design and validation of an SNP-based assay for detecting Juglans ailantifolia introgression into Juglans cinerea populations or living collections
    Article Snippet: lignment of the original CAPS primers with the chloroplast genomes of J. cinerea, J. ailantifolia, J. nigra, J. regia, and Juglans mandshurica L. (Dong et al. 2017). Genotyping was performed using the Agena Bioscience iPLEX Gold platform at the Genome Quebec service facility (Montreal, Canada). In short, a multiplex PCR was performed on 20 ng of template genomic DNA in a 5uL reaction mixture contai



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    During transforming growth factor β1 (TGFβ1)-induced epithelial-to-mesenchymal transition (EMT), stomatin was downregulated through methylation-dependent transcription mechanism. (A) Real-time polymerase chain reaction (PCR) was performed to validate the transcriptional change of stomatin identified in the microarray. **, P<0.001; (B) Two CpG islands spanning from −768 to −223 bp and from −143 to +122 bp upstream of STOM gene were identified by EMBOSS cpgplot program (upper). Schematic representation of −768/−223 and −143/+122 (marked with the grey square) CpG islands in the upstream of stomatin gene (lower). Red box marked the exon. The translation start site was position +1; (C) Measuring stomatin promoter methylation levels by Sequenom MassARRAY assay; (D) Sequenom MassARRAY analysis of methylation percentages of CpG sites at the stomatin promoter. The top panel showed the methylation percentages of 30 sites in human bronchial epithelial (HBE) and A549 cells. Dots with different color depths represent different methylation status. Dark dots represent high degrees of methylation. Light dots represent low degrees of methylation. The methylation percentages of 30 CpG sites were compared by two-tailed paired Student’s t test. In line graph, each dot represents an individual CpG dinucleotide. Green dots represent CpG dinucleotides in HBE cells. Red dots represent CpG sites in A549 cells. The corresponding detection sites were connected in a straight line. Real-time PCR was performed to evaluate the expression of stomatin in HBE and A549 cells (P<0.01); (E) The same sequenom MassArray and statistical analyses were also implemented to assess the methylation status of the indicated promoter region of stomatin in A549 and A549-5Aza cells (P<0.01); (F) A549 cells were exposed to TGFβ1 (5 ng/mL) for 2 d or 5 d with or without 5-Aza. Expression of DNMT3A and stomatin was analyzed by western blotting. β-actin was used as a loading control; (G) Sequenom MassArray analysis was implemented to assess the stomatin promoter methylation levels in A549, A549-TGFβ1, and A549-TGFβ1/5-Aza cells (P=0.005, P=0.004, respectively).

    Journal: Chinese Journal of Cancer Research

    Article Title: Stomatin plays a suppressor role in non-small cell lung cancer metastasis

    doi: 10.21147/j.issn.1000-9604.2019.06.09

    Figure Lengend Snippet: During transforming growth factor β1 (TGFβ1)-induced epithelial-to-mesenchymal transition (EMT), stomatin was downregulated through methylation-dependent transcription mechanism. (A) Real-time polymerase chain reaction (PCR) was performed to validate the transcriptional change of stomatin identified in the microarray. **, P<0.001; (B) Two CpG islands spanning from −768 to −223 bp and from −143 to +122 bp upstream of STOM gene were identified by EMBOSS cpgplot program (upper). Schematic representation of −768/−223 and −143/+122 (marked with the grey square) CpG islands in the upstream of stomatin gene (lower). Red box marked the exon. The translation start site was position +1; (C) Measuring stomatin promoter methylation levels by Sequenom MassARRAY assay; (D) Sequenom MassARRAY analysis of methylation percentages of CpG sites at the stomatin promoter. The top panel showed the methylation percentages of 30 sites in human bronchial epithelial (HBE) and A549 cells. Dots with different color depths represent different methylation status. Dark dots represent high degrees of methylation. Light dots represent low degrees of methylation. The methylation percentages of 30 CpG sites were compared by two-tailed paired Student’s t test. In line graph, each dot represents an individual CpG dinucleotide. Green dots represent CpG dinucleotides in HBE cells. Red dots represent CpG sites in A549 cells. The corresponding detection sites were connected in a straight line. Real-time PCR was performed to evaluate the expression of stomatin in HBE and A549 cells (P<0.01); (E) The same sequenom MassArray and statistical analyses were also implemented to assess the methylation status of the indicated promoter region of stomatin in A549 and A549-5Aza cells (P<0.01); (F) A549 cells were exposed to TGFβ1 (5 ng/mL) for 2 d or 5 d with or without 5-Aza. Expression of DNMT3A and stomatin was analyzed by western blotting. β-actin was used as a loading control; (G) Sequenom MassArray analysis was implemented to assess the stomatin promoter methylation levels in A549, A549-TGFβ1, and A549-TGFβ1/5-Aza cells (P=0.005, P=0.004, respectively).

    Article Snippet: The methylation status of Stomatin promoter was detected using Sequenom MassARRAY quantitative methylation analysis platform (BGI tech, Shenzhen, China).

    Techniques: Methylation, Real-time Polymerase Chain Reaction, Microarray, Sequenom Massarray Assay, Two Tailed Test, Expressing, Western Blot, Control