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massarray platform  (agena bioscience)


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    agena bioscience massarray platform
    DNA Methylation Status of miR-20b-5p by <t>MassARRAY</t> (A) Methylation profile of CpG sites for the miR-20b-5p gene. The color of the circles is related to the percentage of methylation at each CpG site. Boxes indicate the different methylation patterns between 14 ESCC samples and the corresponding tissues. (B) Hierarchical cluster analysis of CpG site methylation profiles of the miR-20b-5p promoter region in esophageal squamous cell carcinoma (n = 14) and control esophageal tumors (n = 14). The color gradient between green and red indicates methylation of each miR-20b-5p unit in each sample ranging from 0% to 60%. Gray represents technically inadequate or missing data. (C) Evaluation of CpG methylation within the miR-20b-5p promoter. The distribution of the seven analyzed CpG units within miR-20b-5p is shown. Data are presented as the mean value ± SD from triplicate experiments. @@ p < 0.01.
    Massarray Platform, supplied by agena bioscience, used in various techniques. Bioz Stars score: 96/100, based on 2219 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/massarray+quantitative+gene+expression+module/Methylation/pmc07334444-279-9-11
    Average 96 stars, based on 2219 article reviews
    massarray platform - by Bioz Stars, 2026-10
    96/100 stars

    Images

    1) Product Images from "Functional Significance and Therapeutic Potential of miRNA-20b-5p in Esophageal Squamous Cell Carcinoma"

    Article Title: Functional Significance and Therapeutic Potential of miRNA-20b-5p in Esophageal Squamous Cell Carcinoma

    Journal: Molecular Therapy. Nucleic Acids

    doi: 10.1016/j.omtn.2020.05.015

    DNA Methylation Status of miR-20b-5p by MassARRAY (A) Methylation profile of CpG sites for the miR-20b-5p gene. The color of the circles is related to the percentage of methylation at each CpG site. Boxes indicate the different methylation patterns between 14 ESCC samples and the corresponding tissues. (B) Hierarchical cluster analysis of CpG site methylation profiles of the miR-20b-5p promoter region in esophageal squamous cell carcinoma (n = 14) and control esophageal tumors (n = 14). The color gradient between green and red indicates methylation of each miR-20b-5p unit in each sample ranging from 0% to 60%. Gray represents technically inadequate or missing data. (C) Evaluation of CpG methylation within the miR-20b-5p promoter. The distribution of the seven analyzed CpG units within miR-20b-5p is shown. Data are presented as the mean value ± SD from triplicate experiments. @@ p < 0.01.
    Figure Legend Snippet: DNA Methylation Status of miR-20b-5p by MassARRAY (A) Methylation profile of CpG sites for the miR-20b-5p gene. The color of the circles is related to the percentage of methylation at each CpG site. Boxes indicate the different methylation patterns between 14 ESCC samples and the corresponding tissues. (B) Hierarchical cluster analysis of CpG site methylation profiles of the miR-20b-5p promoter region in esophageal squamous cell carcinoma (n = 14) and control esophageal tumors (n = 14). The color gradient between green and red indicates methylation of each miR-20b-5p unit in each sample ranging from 0% to 60%. Gray represents technically inadequate or missing data. (C) Evaluation of CpG methylation within the miR-20b-5p promoter. The distribution of the seven analyzed CpG units within miR-20b-5p is shown. Data are presented as the mean value ± SD from triplicate experiments. @@ p < 0.01.

    Techniques Used: DNA Methylation Assay, Methylation, Control, CpG Methylation Assay

    Related Articles

    Polymerase Chain Reaction:

    Article Title: DNA Methylation in Gastric Cancer and Preneoplastic Lesions: Emerging Insights and Future Directions.
    Article Snippet: .. The converted DNA can be analyzed through different PCR-based methods: (i) methylation-specific PCR (MSP) [82,83]; (ii) methylation-sensitive high-resolution technology (MS-HRM) [81,84]; (iii) bisulfite sequencing PCR [76]; (iv) bisulfite pyrosequencing [85–87]; (v) droplet digital PCR (ddPCR) [88–90]; (vi) Epityper by Agena Bioscience (Sequenom, San Diego, CA) [91]. ..

    Article Title: DNA Methylation in Gastric Cancer and Preneoplastic Lesions: Emerging Insights and Future Directions
    Article Snippet: .. The converted DNA can be analyzed through different PCR-based methods: (i) methylation-specific PCR (MSP) [ , ]; (ii) methylation-sensitive high-resolution technology (MS-HRM) [ , ]; (iii) bisulfite sequencing PCR [ ]; (iv) bisulfite pyrosequencing [ , , ]; (v) droplet digital PCR (ddPCR) [ , , ]; (vi) Epityper by Agena Bioscience (Sequenom, San Diego, CA) [ ]. ..

    Methylation:

    Article Title: DNA Methylation in Gastric Cancer and Preneoplastic Lesions: Emerging Insights and Future Directions.
    Article Snippet: .. The converted DNA can be analyzed through different PCR-based methods: (i) methylation-specific PCR (MSP) [82,83]; (ii) methylation-sensitive high-resolution technology (MS-HRM) [81,84]; (iii) bisulfite sequencing PCR [76]; (iv) bisulfite pyrosequencing [85–87]; (v) droplet digital PCR (ddPCR) [88–90]; (vi) Epityper by Agena Bioscience (Sequenom, San Diego, CA) [91]. ..

    Article Title: DNA Methylation in Gastric Cancer and Preneoplastic Lesions: Emerging Insights and Future Directions
    Article Snippet: .. The converted DNA can be analyzed through different PCR-based methods: (i) methylation-specific PCR (MSP) [ , ]; (ii) methylation-sensitive high-resolution technology (MS-HRM) [ , ]; (iii) bisulfite sequencing PCR [ ]; (iv) bisulfite pyrosequencing [ , , ]; (v) droplet digital PCR (ddPCR) [ , , ]; (vi) Epityper by Agena Bioscience (Sequenom, San Diego, CA) [ ]. ..

    Article Title: Differential miRNA expression during replicative senescence of dental pulp stem cells with potential for forensic age assessment.
    Article Snippet: .. Freire-Aradas A, Phillips C, Mosquera-Miguel A, Girón-Santamaría L, Gómez-Tato A, Casares De Cal M, et al. Development of a methylation marker set for forensic age estimation using analysis of public methylation data and the Agena Bioscience EpiTYPER system. ..

    Methylation Sequencing:

    Article Title: DNA Methylation in Gastric Cancer and Preneoplastic Lesions: Emerging Insights and Future Directions.
    Article Snippet: .. The converted DNA can be analyzed through different PCR-based methods: (i) methylation-specific PCR (MSP) [82,83]; (ii) methylation-sensitive high-resolution technology (MS-HRM) [81,84]; (iii) bisulfite sequencing PCR [76]; (iv) bisulfite pyrosequencing [85–87]; (v) droplet digital PCR (ddPCR) [88–90]; (vi) Epityper by Agena Bioscience (Sequenom, San Diego, CA) [91]. ..

    Article Title: DNA Methylation in Gastric Cancer and Preneoplastic Lesions: Emerging Insights and Future Directions
    Article Snippet: .. The converted DNA can be analyzed through different PCR-based methods: (i) methylation-specific PCR (MSP) [ , ]; (ii) methylation-sensitive high-resolution technology (MS-HRM) [ , ]; (iii) bisulfite sequencing PCR [ ]; (iv) bisulfite pyrosequencing [ , , ]; (v) droplet digital PCR (ddPCR) [ , , ]; (vi) Epityper by Agena Bioscience (Sequenom, San Diego, CA) [ ]. ..

    Digital PCR:

    Article Title: DNA Methylation in Gastric Cancer and Preneoplastic Lesions: Emerging Insights and Future Directions.
    Article Snippet: .. The converted DNA can be analyzed through different PCR-based methods: (i) methylation-specific PCR (MSP) [82,83]; (ii) methylation-sensitive high-resolution technology (MS-HRM) [81,84]; (iii) bisulfite sequencing PCR [76]; (iv) bisulfite pyrosequencing [85–87]; (v) droplet digital PCR (ddPCR) [88–90]; (vi) Epityper by Agena Bioscience (Sequenom, San Diego, CA) [91]. ..

    Article Title: DNA Methylation in Gastric Cancer and Preneoplastic Lesions: Emerging Insights and Future Directions
    Article Snippet: .. The converted DNA can be analyzed through different PCR-based methods: (i) methylation-specific PCR (MSP) [ , ]; (ii) methylation-sensitive high-resolution technology (MS-HRM) [ , ]; (iii) bisulfite sequencing PCR [ ]; (iv) bisulfite pyrosequencing [ , , ]; (v) droplet digital PCR (ddPCR) [ , , ]; (vi) Epityper by Agena Bioscience (Sequenom, San Diego, CA) [ ]. ..

    Marker:

    Article Title: Differential miRNA expression during replicative senescence of dental pulp stem cells with potential for forensic age assessment.
    Article Snippet: .. Freire-Aradas A, Phillips C, Mosquera-Miguel A, Girón-Santamaría L, Gómez-Tato A, Casares De Cal M, et al. Development of a methylation marker set for forensic age estimation using analysis of public methylation data and the Agena Bioscience EpiTYPER system. ..

    other:

    Article Title: Chronic heart failure and GPX3 promoter methylation: A clinical-epigenetic analysis.
    Article Snippet: Background: Selenoprotein GPX3 is linked to Chronic Heart Failure (CHF), but its promoter methylation patterns in CHF remain unclear.. Objective: To explore CpG methylation in the GPX3 promoter region and its association with clinical parameters in CHF.. Methods: Twenty CHF patients and twenty healthy controls were included.

    DNA Methylation Assay:

    Article Title: ADAMTS9-AS2 acts as an epigenetic brake to constrain DNMT3B-mediated CADM2 silencing in esophageal squamous cell carcinoma metastasis
    Article Snippet: .. Targeted DNA methylation validation was performed using the MassARRAY EpiTYPER platform (Agena Bioscience) on 24 paired ESCC tumor and adjacent normal tissues. .. Genomic DNA underwent bisulfite conversion (EZ DNA Methylation-Lightning Kit, Zymo Research), followed by PCR amplification of the CADM2 locus flanking the cg03455765 region.

    Biomarker Discovery:

    Article Title: ADAMTS9-AS2 acts as an epigenetic brake to constrain DNMT3B-mediated CADM2 silencing in esophageal squamous cell carcinoma metastasis
    Article Snippet: .. Targeted DNA methylation validation was performed using the MassARRAY EpiTYPER platform (Agena Bioscience) on 24 paired ESCC tumor and adjacent normal tissues. .. Genomic DNA underwent bisulfite conversion (EZ DNA Methylation-Lightning Kit, Zymo Research), followed by PCR amplification of the CADM2 locus flanking the cg03455765 region.



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