dna blood mini kit (Qiagen)
99
Structured Review
Qiagen
dna blood mini kit
Dna Blood Mini Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 3359 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/massarray+analysis+50+snps/QIAamp+DNA+Blood+Mini+Kit/pm30528655-53-96-100
Average 99 stars, based on 3359 article reviews
Dna Blood Mini Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 3359 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/massarray+analysis+50+snps/QIAamp+DNA+Blood+Mini+Kit/pm30528655-53-96-100
Average 99 stars, based on 3359 article reviews
dna blood mini kit - by Bioz Stars,
2026-10
99/100 stars
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Dot Blot:Article Title: Increased vulnerability of colorectal cancer models with high alkylation damage to NTHL1 inactivation. Article Snippet: The plates were then stained with a 0.5% crystal violet solution (C0775-25G, Millipore Sigma, Burlington, MA) for 10 minutes. .. Dot blot assay on 3-methylcytosine (3-MeC) AR TIC LE IN Sequencing:Article Title: Meiotic gene variants contribute to recurrent blastulation failure Article Snippet: .. 166 Whole-exome sequencing and variant analysis 167 Genomic DNA was extracted from peripheral blood samples using Variant Assay:Article Title: Meiotic gene variants contribute to recurrent blastulation failure Article Snippet: .. 166 Whole-exome sequencing and variant analysis 167 Genomic DNA was extracted from peripheral blood samples using Recombinant:Article Title: Presentation of identical tumor antigen peptides by canine and human MHC class I Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-MHC Class I (HLA-A, HLA-B, HLA-C) – human, Clone W6/32 BioXCell Cat# BE0079; RRID: AB_1107730 Mouse anti-MHC class I monoclonal antibody, Clone DG-H58A Monoclonal Antibody Center, Washington State University Cat# DG-BOV2001; RRID: AB_3718131 Anti-Human HLA A2, B7 (MHC Class I), Clone BB7.6 Leinco Technologies RRID: AB_2893725 Bacterial and virus strains OneShot Top10 Chemically Competent ThermoFisher Cat# C404003 Biological samples Canine tumor samples Collected from veterinary hospitals with owner informed consent. .. N/A Chemicals, peptides, and recombinant proteins RPMI 1640 Gibco Cat# 11875119 DMEM/F-12 Gibco Cat# 11320082 PBS Gibco Cat# 20012027 FBS Gibco Cat# A5256701 CNBr-activated Sepharose 4 Fast Flow Amersham Pharmacia Biotech/Cytiva Cat# 17-0981-01 Tris pH 8.0 NELS Cat# E199-500 ML IGEPAL® CA-630 Sigma-Aldrich Cat# 18896 Complete protease inhibitor cocktail Roche Cat# 11836145001 Glacial Acetic acid Sigma-Aldrich Cat# AX0077 iRT peptides Biognosys Cat# 1900615 Transporter 5 Polysciences Cat# 26008 Xho1 NEB Cat# R0146S Salt-T4 DNA Ligase NEB Cat# M0467S EcoR1 NEB Cat# R3101S LR Clonase II ThermoFisher Cat# 11791020 Polybrene Sigma-Aldrich Cat# TR-1003-50UL Blasticidin ThermoFisher Cat# A1113903 Critical commercial assays MelonTM Gel IgG Spin Purification Kit Thermo Scientific Cat# 45206 ZebaTM Spin Desalting Columns Thermo Scientific Cat# 89893 QIAfilter Plasmid Kits Qiagen Cat #12243 Monarch Plasmid Spin Miniprep Kit NEB Cat #T1110L Monarch Spin DNA Gel Extraction Kit NEB Cat #T1120L Article Title: AAV-based gene therapy with modified HEXB confers lasting therapeutic benefits in GM2 gangliosidosis models. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies GM2-specific mouse mAb (GMB28: IgM) Provided by Dr. Tadashi Tai Kotani et al.65 Anti-NAG(A), antiserum against human HexA Provided by Dr. Akihiko Tsuji Izumi et al.,66 Utsumi et al.67 Anti-GFP antibody (JL-8) Takara Bio Cat# 632381; RRID: AB_2313808 anti-NeuN antibody Merck Millipore Cat# ABN78; RRID: AB_10807945 anti-GAPDH antibody (6C5) Santa Cruz Biotechnology Cat# ab8245; RRID: AB_2107448 anti-HexB antibody Abcam Cat# ab140649; RRID: AB_3065101 Anti-CD68 antibody (FA-11) Thermo Fisher Scientific Cat# 14-0681-82; RRID: AB_2572857 Anti-rabbit IgG, HRP-linked Antibody Cell Signaling Technology Cat# 7074; RRID: AB_2099233 Anti-mouse IgG, HRP-linked Antibody Cell Signaling Technology Cat# 7076; RRID: AB_330924 Goat Anti-Mouse IgG+IgM H&L (FITC) preadsorbed Abcam Cat# ab47830; RRID: AB_955209 Chemicals, peptides, and recombinant proteins 4-methylumbelliferyl-N-acetyl-β-D-glucosaminide Sigma-Aldrich Cat# 474502 4-methylumbelliferyl-6-sulfo-N-acetyl-β-D-glucosaminide Merck Cat# 454428 Blocking One Nacalai Tesque Cat# 03953-95 Western Lightning Plus-ECL Perkin Elmer Cat# NEL104001EA Western Lightning Ultra Perkin Elmer Cat# NEL111001EA Hoechst33258 Sigma-Aldrich Cat# 94403 Tissue-Tek O.C.T. compound Sakura Finetek Japan Cat# 4583 HistoVT One Nacalai Tesque Cat# 06380-76 antigen retrieval buffer Abcam Cat# ab93684 AS-BI N-acetyl-β-D-glucosaminide Merck Cat# N4006 Critical commercial assays DC Protein Assay Bio-Rad Cat# 5000112 DeadEnd Colorimetric TUNEL system Promega Cat# G7360 VECTASTAIN Elite ABC Kit Vector Laboratories Cat# PK-6100 Tissue-Tek Mayer Hematoxylin For Prisma standard solution reservoir Sakura Finetek Japan Cat# 6186-4P Mouse CCL3/MIP-1 alpha ELISA Kit - Quantikine R&D Systems Cat# Protease Inhibitor:Article Title: Presentation of identical tumor antigen peptides by canine and human MHC class I Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-MHC Class I (HLA-A, HLA-B, HLA-C) – human, Clone W6/32 BioXCell Cat# BE0079; RRID: AB_1107730 Mouse anti-MHC class I monoclonal antibody, Clone DG-H58A Monoclonal Antibody Center, Washington State University Cat# DG-BOV2001; RRID: AB_3718131 Anti-Human HLA A2, B7 (MHC Class I), Clone BB7.6 Leinco Technologies RRID: AB_2893725 Bacterial and virus strains OneShot Top10 Chemically Competent ThermoFisher Cat# C404003 Biological samples Canine tumor samples Collected from veterinary hospitals with owner informed consent. .. N/A Chemicals, peptides, and recombinant proteins RPMI 1640 Gibco Cat# 11875119 DMEM/F-12 Gibco Cat# 11320082 PBS Gibco Cat# 20012027 FBS Gibco Cat# A5256701 CNBr-activated Sepharose 4 Fast Flow Amersham Pharmacia Biotech/Cytiva Cat# 17-0981-01 Tris pH 8.0 NELS Cat# E199-500 ML IGEPAL® CA-630 Sigma-Aldrich Cat# 18896 Complete protease inhibitor cocktail Roche Cat# 11836145001 Glacial Acetic acid Sigma-Aldrich Cat# AX0077 iRT peptides Biognosys Cat# 1900615 Transporter 5 Polysciences Cat# 26008 Xho1 NEB Cat# R0146S Salt-T4 DNA Ligase NEB Cat# M0467S EcoR1 NEB Cat# R3101S LR Clonase II ThermoFisher Cat# 11791020 Polybrene Sigma-Aldrich Cat# TR-1003-50UL Blasticidin ThermoFisher Cat# A1113903 Critical commercial assays MelonTM Gel IgG Spin Purification Kit Thermo Scientific Cat# 45206 ZebaTM Spin Desalting Columns Thermo Scientific Cat# 89893 QIAfilter Plasmid Kits Qiagen Cat #12243 Monarch Plasmid Spin Miniprep Kit NEB Cat #T1110L Monarch Spin DNA Gel Extraction Kit NEB Cat #T1120L Purification:Article Title: Presentation of identical tumor antigen peptides by canine and human MHC class I Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-MHC Class I (HLA-A, HLA-B, HLA-C) – human, Clone W6/32 BioXCell Cat# BE0079; RRID: AB_1107730 Mouse anti-MHC class I monoclonal antibody, Clone DG-H58A Monoclonal Antibody Center, Washington State University Cat# DG-BOV2001; RRID: AB_3718131 Anti-Human HLA A2, B7 (MHC Class I), Clone BB7.6 Leinco Technologies RRID: AB_2893725 Bacterial and virus strains OneShot Top10 Chemically Competent ThermoFisher Cat# C404003 Biological samples Canine tumor samples Collected from veterinary hospitals with owner informed consent. .. N/A Chemicals, peptides, and recombinant proteins RPMI 1640 Gibco Cat# 11875119 DMEM/F-12 Gibco Cat# 11320082 PBS Gibco Cat# 20012027 FBS Gibco Cat# A5256701 CNBr-activated Sepharose 4 Fast Flow Amersham Pharmacia Biotech/Cytiva Cat# 17-0981-01 Tris pH 8.0 NELS Cat# E199-500 ML IGEPAL® CA-630 Sigma-Aldrich Cat# 18896 Complete protease inhibitor cocktail Roche Cat# 11836145001 Glacial Acetic acid Sigma-Aldrich Cat# AX0077 iRT peptides Biognosys Cat# 1900615 Transporter 5 Polysciences Cat# 26008 Xho1 NEB Cat# R0146S Salt-T4 DNA Ligase NEB Cat# M0467S EcoR1 NEB Cat# R3101S LR Clonase II ThermoFisher Cat# 11791020 Polybrene Sigma-Aldrich Cat# TR-1003-50UL Blasticidin ThermoFisher Cat# A1113903 Critical commercial assays MelonTM Gel IgG Spin Purification Kit Thermo Scientific Cat# 45206 ZebaTM Spin Desalting Columns Thermo Scientific Cat# 89893 QIAfilter Plasmid Kits Qiagen Cat #12243 Monarch Plasmid Spin Miniprep Kit NEB Cat #T1110L Monarch Spin DNA Gel Extraction Kit NEB Cat #T1120L Plasmid Preparation:Article Title: Presentation of identical tumor antigen peptides by canine and human MHC class I Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-MHC Class I (HLA-A, HLA-B, HLA-C) – human, Clone W6/32 BioXCell Cat# BE0079; RRID: AB_1107730 Mouse anti-MHC class I monoclonal antibody, Clone DG-H58A Monoclonal Antibody Center, Washington State University Cat# DG-BOV2001; RRID: AB_3718131 Anti-Human HLA A2, B7 (MHC Class I), Clone BB7.6 Leinco Technologies RRID: AB_2893725 Bacterial and virus strains OneShot Top10 Chemically Competent ThermoFisher Cat# C404003 Biological samples Canine tumor samples Collected from veterinary hospitals with owner informed consent. .. N/A Chemicals, peptides, and recombinant proteins RPMI 1640 Gibco Cat# 11875119 DMEM/F-12 Gibco Cat# 11320082 PBS Gibco Cat# 20012027 FBS Gibco Cat# A5256701 CNBr-activated Sepharose 4 Fast Flow Amersham Pharmacia Biotech/Cytiva Cat# 17-0981-01 Tris pH 8.0 NELS Cat# E199-500 ML IGEPAL® CA-630 Sigma-Aldrich Cat# 18896 Complete protease inhibitor cocktail Roche Cat# 11836145001 Glacial Acetic acid Sigma-Aldrich Cat# AX0077 iRT peptides Biognosys Cat# 1900615 Transporter 5 Polysciences Cat# 26008 Xho1 NEB Cat# R0146S Salt-T4 DNA Ligase NEB Cat# M0467S EcoR1 NEB Cat# R3101S LR Clonase II ThermoFisher Cat# 11791020 Polybrene Sigma-Aldrich Cat# TR-1003-50UL Blasticidin ThermoFisher Cat# A1113903 Critical commercial assays MelonTM Gel IgG Spin Purification Kit Thermo Scientific Cat# 45206 ZebaTM Spin Desalting Columns Thermo Scientific Cat# 89893 QIAfilter Plasmid Kits Qiagen Cat #12243 Monarch Plasmid Spin Miniprep Kit NEB Cat #T1110L Monarch Spin DNA Gel Extraction Kit NEB Cat #T1120L Gel Extraction:Article Title: Presentation of identical tumor antigen peptides by canine and human MHC class I Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-MHC Class I (HLA-A, HLA-B, HLA-C) – human, Clone W6/32 BioXCell Cat# BE0079; RRID: AB_1107730 Mouse anti-MHC class I monoclonal antibody, Clone DG-H58A Monoclonal Antibody Center, Washington State University Cat# DG-BOV2001; RRID: AB_3718131 Anti-Human HLA A2, B7 (MHC Class I), Clone BB7.6 Leinco Technologies RRID: AB_2893725 Bacterial and virus strains OneShot Top10 Chemically Competent ThermoFisher Cat# C404003 Biological samples Canine tumor samples Collected from veterinary hospitals with owner informed consent. .. N/A Chemicals, peptides, and recombinant proteins RPMI 1640 Gibco Cat# 11875119 DMEM/F-12 Gibco Cat# 11320082 PBS Gibco Cat# 20012027 FBS Gibco Cat# A5256701 CNBr-activated Sepharose 4 Fast Flow Amersham Pharmacia Biotech/Cytiva Cat# 17-0981-01 Tris pH 8.0 NELS Cat# E199-500 ML IGEPAL® CA-630 Sigma-Aldrich Cat# 18896 Complete protease inhibitor cocktail Roche Cat# 11836145001 Glacial Acetic acid Sigma-Aldrich Cat# AX0077 iRT peptides Biognosys Cat# 1900615 Transporter 5 Polysciences Cat# 26008 Xho1 NEB Cat# R0146S Salt-T4 DNA Ligase NEB Cat# M0467S EcoR1 NEB Cat# R3101S LR Clonase II ThermoFisher Cat# 11791020 Polybrene Sigma-Aldrich Cat# TR-1003-50UL Blasticidin ThermoFisher Cat# A1113903 Critical commercial assays MelonTM Gel IgG Spin Purification Kit Thermo Scientific Cat# 45206 ZebaTM Spin Desalting Columns Thermo Scientific Cat# 89893 QIAfilter Plasmid Kits Qiagen Cat #12243 Monarch Plasmid Spin Miniprep Kit NEB Cat #T1110L Monarch Spin DNA Gel Extraction Kit NEB Cat #T1120L Mass Spectrometry:Article Title: Presentation of identical tumor antigen peptides by canine and human MHC class I Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-MHC Class I (HLA-A, HLA-B, HLA-C) – human, Clone W6/32 BioXCell Cat# BE0079; RRID: AB_1107730 Mouse anti-MHC class I monoclonal antibody, Clone DG-H58A Monoclonal Antibody Center, Washington State University Cat# DG-BOV2001; RRID: AB_3718131 Anti-Human HLA A2, B7 (MHC Class I), Clone BB7.6 Leinco Technologies RRID: AB_2893725 Bacterial and virus strains OneShot Top10 Chemically Competent ThermoFisher Cat# C404003 Biological samples Canine tumor samples Collected from veterinary hospitals with owner informed consent. .. N/A Chemicals, peptides, and recombinant proteins RPMI 1640 Gibco Cat# 11875119 DMEM/F-12 Gibco Cat# 11320082 PBS Gibco Cat# 20012027 FBS Gibco Cat# A5256701 CNBr-activated Sepharose 4 Fast Flow Amersham Pharmacia Biotech/Cytiva Cat# 17-0981-01 Tris pH 8.0 NELS Cat# E199-500 ML IGEPAL® CA-630 Sigma-Aldrich Cat# 18896 Complete protease inhibitor cocktail Roche Cat# 11836145001 Glacial Acetic acid Sigma-Aldrich Cat# AX0077 iRT peptides Biognosys Cat# 1900615 Transporter 5 Polysciences Cat# 26008 Xho1 NEB Cat# R0146S Salt-T4 DNA Ligase NEB Cat# M0467S EcoR1 NEB Cat# R3101S LR Clonase II ThermoFisher Cat# 11791020 Polybrene Sigma-Aldrich Cat# TR-1003-50UL Blasticidin ThermoFisher Cat# A1113903 Critical commercial assays MelonTM Gel IgG Spin Purification Kit Thermo Scientific Cat# 45206 ZebaTM Spin Desalting Columns Thermo Scientific Cat# 89893 QIAfilter Plasmid Kits Qiagen Cat #12243 Monarch Plasmid Spin Miniprep Kit NEB Cat #T1110L Monarch Spin DNA Gel Extraction Kit NEB Cat #T1120L RNA Sequencing:Article Title: Presentation of identical tumor antigen peptides by canine and human MHC class I Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-MHC Class I (HLA-A, HLA-B, HLA-C) – human, Clone W6/32 BioXCell Cat# BE0079; RRID: AB_1107730 Mouse anti-MHC class I monoclonal antibody, Clone DG-H58A Monoclonal Antibody Center, Washington State University Cat# DG-BOV2001; RRID: AB_3718131 Anti-Human HLA A2, B7 (MHC Class I), Clone BB7.6 Leinco Technologies RRID: AB_2893725 Bacterial and virus strains OneShot Top10 Chemically Competent ThermoFisher Cat# C404003 Biological samples Canine tumor samples Collected from veterinary hospitals with owner informed consent. .. N/A Chemicals, peptides, and recombinant proteins RPMI 1640 Gibco Cat# 11875119 DMEM/F-12 Gibco Cat# 11320082 PBS Gibco Cat# 20012027 FBS Gibco Cat# A5256701 CNBr-activated Sepharose 4 Fast Flow Amersham Pharmacia Biotech/Cytiva Cat# 17-0981-01 Tris pH 8.0 NELS Cat# E199-500 ML IGEPAL® CA-630 Sigma-Aldrich Cat# 18896 Complete protease inhibitor cocktail Roche Cat# 11836145001 Glacial Acetic acid Sigma-Aldrich Cat# AX0077 iRT peptides Biognosys Cat# 1900615 Transporter 5 Polysciences Cat# 26008 Xho1 NEB Cat# R0146S Salt-T4 DNA Ligase NEB Cat# M0467S EcoR1 NEB Cat# R3101S LR Clonase II ThermoFisher Cat# 11791020 Polybrene Sigma-Aldrich Cat# TR-1003-50UL Blasticidin ThermoFisher Cat# A1113903 Critical commercial assays MelonTM Gel IgG Spin Purification Kit Thermo Scientific Cat# 45206 ZebaTM Spin Desalting Columns Thermo Scientific Cat# 89893 QIAfilter Plasmid Kits Qiagen Cat #12243 Monarch Plasmid Spin Miniprep Kit NEB Cat #T1110L Monarch Spin DNA Gel Extraction Kit NEB Cat #T1120L Isolation:Article Title: Heparanase (HPSE) genetic variants as prognostic indicators in ovarian cancer: evidence from discovery and validation cohorts Article Snippet: .. DNA was isolated using the Cell Culture:Article Title: Heparanase (HPSE) genetic variants as prognostic indicators in ovarian cancer: evidence from discovery and validation cohorts Article Snippet: .. DNA was isolated using the RNA Extraction:Article Title: Comprehensive Molecular Characterization of High-Grade Endometrial Cancer in an Ancestrally Diverse Cohort Article Snippet: The isolated PBMCs were cryopreserved following resuspension in Recovery Cell Culture Freezing Medium (ThermoFisher, 12648010). .. DNA and RNA extraction was primarily conducted at NYGC using the following protocol: PBMC normal samples were extracted with the Blocking Assay:Article Title: AAV-based gene therapy with modified HEXB confers lasting therapeutic benefits in GM2 gangliosidosis models. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies GM2-specific mouse mAb (GMB28: IgM) Provided by Dr. Tadashi Tai Kotani et al.65 Anti-NAG(A), antiserum against human HexA Provided by Dr. Akihiko Tsuji Izumi et al.,66 Utsumi et al.67 Anti-GFP antibody (JL-8) Takara Bio Cat# 632381; RRID: AB_2313808 anti-NeuN antibody Merck Millipore Cat# ABN78; RRID: AB_10807945 anti-GAPDH antibody (6C5) Santa Cruz Biotechnology Cat# ab8245; RRID: AB_2107448 anti-HexB antibody Abcam Cat# ab140649; RRID: AB_3065101 Anti-CD68 antibody (FA-11) Thermo Fisher Scientific Cat# 14-0681-82; RRID: AB_2572857 Anti-rabbit IgG, HRP-linked Antibody Cell Signaling Technology Cat# 7074; RRID: AB_2099233 Anti-mouse IgG, HRP-linked Antibody Cell Signaling Technology Cat# 7076; RRID: AB_330924 Goat Anti-Mouse IgG+IgM H&L (FITC) preadsorbed Abcam Cat# ab47830; RRID: AB_955209 Chemicals, peptides, and recombinant proteins 4-methylumbelliferyl-N-acetyl-β-D-glucosaminide Sigma-Aldrich Cat# 474502 4-methylumbelliferyl-6-sulfo-N-acetyl-β-D-glucosaminide Merck Cat# 454428 Blocking One Nacalai Tesque Cat# 03953-95 Western Lightning Plus-ECL Perkin Elmer Cat# NEL104001EA Western Lightning Ultra Perkin Elmer Cat# NEL111001EA Hoechst33258 Sigma-Aldrich Cat# 94403 Tissue-Tek O.C.T. compound Sakura Finetek Japan Cat# 4583 HistoVT One Nacalai Tesque Cat# 06380-76 antigen retrieval buffer Abcam Cat# ab93684 AS-BI N-acetyl-β-D-glucosaminide Merck Cat# N4006 Critical commercial assays DC Protein Assay Bio-Rad Cat# 5000112 DeadEnd Colorimetric TUNEL system Promega Cat# G7360 VECTASTAIN Elite ABC Kit Vector Laboratories Cat# PK-6100 Tissue-Tek Mayer Hematoxylin For Prisma standard solution reservoir Sakura Finetek Japan Cat# 6186-4P Mouse CCL3/MIP-1 alpha ELISA Kit - Quantikine R&D Systems Cat# Western Blot:Article Title: AAV-based gene therapy with modified HEXB confers lasting therapeutic benefits in GM2 gangliosidosis models. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies GM2-specific mouse mAb (GMB28: IgM) Provided by Dr. Tadashi Tai Kotani et al.65 Anti-NAG(A), antiserum against human HexA Provided by Dr. Akihiko Tsuji Izumi et al.,66 Utsumi et al.67 Anti-GFP antibody (JL-8) Takara Bio Cat# 632381; RRID: AB_2313808 anti-NeuN antibody Merck Millipore Cat# ABN78; RRID: AB_10807945 anti-GAPDH antibody (6C5) Santa Cruz Biotechnology Cat# ab8245; RRID: AB_2107448 anti-HexB antibody Abcam Cat# ab140649; RRID: AB_3065101 Anti-CD68 antibody (FA-11) Thermo Fisher Scientific Cat# 14-0681-82; RRID: AB_2572857 Anti-rabbit IgG, HRP-linked Antibody Cell Signaling Technology Cat# 7074; RRID: AB_2099233 Anti-mouse IgG, HRP-linked Antibody Cell Signaling Technology Cat# 7076; RRID: AB_330924 Goat Anti-Mouse IgG+IgM H&L (FITC) preadsorbed Abcam Cat# ab47830; RRID: AB_955209 Chemicals, peptides, and recombinant proteins 4-methylumbelliferyl-N-acetyl-β-D-glucosaminide Sigma-Aldrich Cat# 474502 4-methylumbelliferyl-6-sulfo-N-acetyl-β-D-glucosaminide Merck Cat# 454428 Blocking One Nacalai Tesque Cat# 03953-95 Western Lightning Plus-ECL Perkin Elmer Cat# NEL104001EA Western Lightning Ultra Perkin Elmer Cat# NEL111001EA Hoechst33258 Sigma-Aldrich Cat# 94403 Tissue-Tek O.C.T. compound Sakura Finetek Japan Cat# 4583 HistoVT One Nacalai Tesque Cat# 06380-76 antigen retrieval buffer Abcam Cat# ab93684 AS-BI N-acetyl-β-D-glucosaminide Merck Cat# N4006 Critical commercial assays DC Protein Assay Bio-Rad Cat# 5000112 DeadEnd Colorimetric TUNEL system Promega Cat# G7360 VECTASTAIN Elite ABC Kit Vector Laboratories Cat# PK-6100 Tissue-Tek Mayer Hematoxylin For Prisma standard solution reservoir Sakura Finetek Japan Cat# 6186-4P Mouse CCL3/MIP-1 alpha ELISA Kit - Quantikine R&D Systems Cat# DC Protein Assay:Article Title: AAV-based gene therapy with modified HEXB confers lasting therapeutic benefits in GM2 gangliosidosis models. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies GM2-specific mouse mAb (GMB28: IgM) Provided by Dr. Tadashi Tai Kotani et al.65 Anti-NAG(A), antiserum against human HexA Provided by Dr. Akihiko Tsuji Izumi et al.,66 Utsumi et al.67 Anti-GFP antibody (JL-8) Takara Bio Cat# 632381; RRID: AB_2313808 anti-NeuN antibody Merck Millipore Cat# ABN78; RRID: AB_10807945 anti-GAPDH antibody (6C5) Santa Cruz Biotechnology Cat# ab8245; RRID: AB_2107448 anti-HexB antibody Abcam Cat# ab140649; RRID: AB_3065101 Anti-CD68 antibody (FA-11) Thermo Fisher Scientific Cat# 14-0681-82; RRID: AB_2572857 Anti-rabbit IgG, HRP-linked Antibody Cell Signaling Technology Cat# 7074; RRID: AB_2099233 Anti-mouse IgG, HRP-linked Antibody Cell Signaling Technology Cat# 7076; RRID: AB_330924 Goat Anti-Mouse IgG+IgM H&L (FITC) preadsorbed Abcam Cat# ab47830; RRID: AB_955209 Chemicals, peptides, and recombinant proteins 4-methylumbelliferyl-N-acetyl-β-D-glucosaminide Sigma-Aldrich Cat# 474502 4-methylumbelliferyl-6-sulfo-N-acetyl-β-D-glucosaminide Merck Cat# 454428 Blocking One Nacalai Tesque Cat# 03953-95 Western Lightning Plus-ECL Perkin Elmer Cat# NEL104001EA Western Lightning Ultra Perkin Elmer Cat# NEL111001EA Hoechst33258 Sigma-Aldrich Cat# 94403 Tissue-Tek O.C.T. compound Sakura Finetek Japan Cat# 4583 HistoVT One Nacalai Tesque Cat# 06380-76 antigen retrieval buffer Abcam Cat# ab93684 AS-BI N-acetyl-β-D-glucosaminide Merck Cat# N4006 Critical commercial assays DC Protein Assay Bio-Rad Cat# 5000112 DeadEnd Colorimetric TUNEL system Promega Cat# G7360 VECTASTAIN Elite ABC Kit Vector Laboratories Cat# PK-6100 Tissue-Tek Mayer Hematoxylin For Prisma standard solution reservoir Sakura Finetek Japan Cat# 6186-4P Mouse CCL3/MIP-1 alpha ELISA Kit - Quantikine R&D Systems Cat# TUNEL Assay:Article Title: AAV-based gene therapy with modified HEXB confers lasting therapeutic benefits in GM2 gangliosidosis models. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies GM2-specific mouse mAb (GMB28: IgM) Provided by Dr. Tadashi Tai Kotani et al.65 Anti-NAG(A), antiserum against human HexA Provided by Dr. Akihiko Tsuji Izumi et al.,66 Utsumi et al.67 Anti-GFP antibody (JL-8) Takara Bio Cat# 632381; RRID: AB_2313808 anti-NeuN antibody Merck Millipore Cat# ABN78; RRID: AB_10807945 anti-GAPDH antibody (6C5) Santa Cruz Biotechnology Cat# ab8245; RRID: AB_2107448 anti-HexB antibody Abcam Cat# ab140649; RRID: AB_3065101 Anti-CD68 antibody (FA-11) Thermo Fisher Scientific Cat# 14-0681-82; RRID: AB_2572857 Anti-rabbit IgG, HRP-linked Antibody Cell Signaling Technology Cat# 7074; RRID: AB_2099233 Anti-mouse IgG, HRP-linked Antibody Cell Signaling Technology Cat# 7076; RRID: AB_330924 Goat Anti-Mouse IgG+IgM H&L (FITC) preadsorbed Abcam Cat# ab47830; RRID: AB_955209 Chemicals, peptides, and recombinant proteins 4-methylumbelliferyl-N-acetyl-β-D-glucosaminide Sigma-Aldrich Cat# 474502 4-methylumbelliferyl-6-sulfo-N-acetyl-β-D-glucosaminide Merck Cat# 454428 Blocking One Nacalai Tesque Cat# 03953-95 Western Lightning Plus-ECL Perkin Elmer Cat# NEL104001EA Western Lightning Ultra Perkin Elmer Cat# NEL111001EA Hoechst33258 Sigma-Aldrich Cat# 94403 Tissue-Tek O.C.T. compound Sakura Finetek Japan Cat# 4583 HistoVT One Nacalai Tesque Cat# 06380-76 antigen retrieval buffer Abcam Cat# ab93684 AS-BI N-acetyl-β-D-glucosaminide Merck Cat# N4006 Critical commercial assays DC Protein Assay Bio-Rad Cat# 5000112 DeadEnd Colorimetric TUNEL system Promega Cat# G7360 VECTASTAIN Elite ABC Kit Vector Laboratories Cat# PK-6100 Tissue-Tek Mayer Hematoxylin For Prisma standard solution reservoir Sakura Finetek Japan Cat# 6186-4P Mouse CCL3/MIP-1 alpha ELISA Kit - Quantikine R&D Systems Cat# Enzyme-linked Immunosorbent Assay:Article Title: AAV-based gene therapy with modified HEXB confers lasting therapeutic benefits in GM2 gangliosidosis models. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies GM2-specific mouse mAb (GMB28: IgM) Provided by Dr. Tadashi Tai Kotani et al.65 Anti-NAG(A), antiserum against human HexA Provided by Dr. Akihiko Tsuji Izumi et al.,66 Utsumi et al.67 Anti-GFP antibody (JL-8) Takara Bio Cat# 632381; RRID: AB_2313808 anti-NeuN antibody Merck Millipore Cat# ABN78; RRID: AB_10807945 anti-GAPDH antibody (6C5) Santa Cruz Biotechnology Cat# ab8245; RRID: AB_2107448 anti-HexB antibody Abcam Cat# ab140649; RRID: AB_3065101 Anti-CD68 antibody (FA-11) Thermo Fisher Scientific Cat# 14-0681-82; RRID: AB_2572857 Anti-rabbit IgG, HRP-linked Antibody Cell Signaling Technology Cat# 7074; RRID: AB_2099233 Anti-mouse IgG, HRP-linked Antibody Cell Signaling Technology Cat# 7076; RRID: AB_330924 Goat Anti-Mouse IgG+IgM H&L (FITC) preadsorbed Abcam Cat# ab47830; RRID: AB_955209 Chemicals, peptides, and recombinant proteins 4-methylumbelliferyl-N-acetyl-β-D-glucosaminide Sigma-Aldrich Cat# 474502 4-methylumbelliferyl-6-sulfo-N-acetyl-β-D-glucosaminide Merck Cat# 454428 Blocking One Nacalai Tesque Cat# 03953-95 Western Lightning Plus-ECL Perkin Elmer Cat# NEL104001EA Western Lightning Ultra Perkin Elmer Cat# NEL111001EA Hoechst33258 Sigma-Aldrich Cat# 94403 Tissue-Tek O.C.T. compound Sakura Finetek Japan Cat# 4583 HistoVT One Nacalai Tesque Cat# 06380-76 antigen retrieval buffer Abcam Cat# ab93684 AS-BI N-acetyl-β-D-glucosaminide Merck Cat# N4006 Critical commercial assays DC Protein Assay Bio-Rad Cat# 5000112 DeadEnd Colorimetric TUNEL system Promega Cat# G7360 VECTASTAIN Elite ABC Kit Vector Laboratories Cat# PK-6100 Tissue-Tek Mayer Hematoxylin For Prisma standard solution reservoir Sakura Finetek Japan Cat# 6186-4P Mouse CCL3/MIP-1 alpha ELISA Kit - Quantikine R&D Systems Cat# Real-time Polymerase Chain Reaction:Article Title: AAV-based gene therapy with modified HEXB confers lasting therapeutic benefits in GM2 gangliosidosis models. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies GM2-specific mouse mAb (GMB28: IgM) Provided by Dr. Tadashi Tai Kotani et al.65 Anti-NAG(A), antiserum against human HexA Provided by Dr. Akihiko Tsuji Izumi et al.,66 Utsumi et al.67 Anti-GFP antibody (JL-8) Takara Bio Cat# 632381; RRID: AB_2313808 anti-NeuN antibody Merck Millipore Cat# ABN78; RRID: AB_10807945 anti-GAPDH antibody (6C5) Santa Cruz Biotechnology Cat# ab8245; RRID: AB_2107448 anti-HexB antibody Abcam Cat# ab140649; RRID: AB_3065101 Anti-CD68 antibody (FA-11) Thermo Fisher Scientific Cat# 14-0681-82; RRID: AB_2572857 Anti-rabbit IgG, HRP-linked Antibody Cell Signaling Technology Cat# 7074; RRID: AB_2099233 Anti-mouse IgG, HRP-linked Antibody Cell Signaling Technology Cat# 7076; RRID: AB_330924 Goat Anti-Mouse IgG+IgM H&L (FITC) preadsorbed Abcam Cat# ab47830; RRID: AB_955209 Chemicals, peptides, and recombinant proteins 4-methylumbelliferyl-N-acetyl-β-D-glucosaminide Sigma-Aldrich Cat# 474502 4-methylumbelliferyl-6-sulfo-N-acetyl-β-D-glucosaminide Merck Cat# 454428 Blocking One Nacalai Tesque Cat# 03953-95 Western Lightning Plus-ECL Perkin Elmer Cat# NEL104001EA Western Lightning Ultra Perkin Elmer Cat# NEL111001EA Hoechst33258 Sigma-Aldrich Cat# 94403 Tissue-Tek O.C.T. compound Sakura Finetek Japan Cat# 4583 HistoVT One Nacalai Tesque Cat# 06380-76 antigen retrieval buffer Abcam Cat# ab93684 AS-BI N-acetyl-β-D-glucosaminide Merck Cat# N4006 Critical commercial assays DC Protein Assay Bio-Rad Cat# 5000112 DeadEnd Colorimetric TUNEL system Promega Cat# G7360 VECTASTAIN Elite ABC Kit Vector Laboratories Cat# PK-6100 Tissue-Tek Mayer Hematoxylin For Prisma standard solution reservoir Sakura Finetek Japan Cat# 6186-4P Mouse CCL3/MIP-1 alpha ELISA Kit - Quantikine R&D Systems Cat# Derivative Assay:Article Title: AAV-based gene therapy with modified HEXB confers lasting therapeutic benefits in GM2 gangliosidosis models. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies GM2-specific mouse mAb (GMB28: IgM) Provided by Dr. Tadashi Tai Kotani et al.65 Anti-NAG(A), antiserum against human HexA Provided by Dr. Akihiko Tsuji Izumi et al.,66 Utsumi et al.67 Anti-GFP antibody (JL-8) Takara Bio Cat# 632381; RRID: AB_2313808 anti-NeuN antibody Merck Millipore Cat# ABN78; RRID: AB_10807945 anti-GAPDH antibody (6C5) Santa Cruz Biotechnology Cat# ab8245; RRID: AB_2107448 anti-HexB antibody Abcam Cat# ab140649; RRID: AB_3065101 Anti-CD68 antibody (FA-11) Thermo Fisher Scientific Cat# 14-0681-82; RRID: AB_2572857 Anti-rabbit IgG, HRP-linked Antibody Cell Signaling Technology Cat# 7074; RRID: AB_2099233 Anti-mouse IgG, HRP-linked Antibody Cell Signaling Technology Cat# 7076; RRID: AB_330924 Goat Anti-Mouse IgG+IgM H&L (FITC) preadsorbed Abcam Cat# ab47830; RRID: AB_955209 Chemicals, peptides, and recombinant proteins 4-methylumbelliferyl-N-acetyl-β-D-glucosaminide Sigma-Aldrich Cat# 474502 4-methylumbelliferyl-6-sulfo-N-acetyl-β-D-glucosaminide Merck Cat# 454428 Blocking One Nacalai Tesque Cat# 03953-95 Western Lightning Plus-ECL Perkin Elmer Cat# NEL104001EA Western Lightning Ultra Perkin Elmer Cat# NEL111001EA Hoechst33258 Sigma-Aldrich Cat# 94403 Tissue-Tek O.C.T. compound Sakura Finetek Japan Cat# 4583 HistoVT One Nacalai Tesque Cat# 06380-76 antigen retrieval buffer Abcam Cat# ab93684 AS-BI N-acetyl-β-D-glucosaminide Merck Cat# N4006 Critical commercial assays DC Protein Assay Bio-Rad Cat# 5000112 DeadEnd Colorimetric TUNEL system Promega Cat# G7360 VECTASTAIN Elite ABC Kit Vector Laboratories Cat# PK-6100 Tissue-Tek Mayer Hematoxylin For Prisma standard solution reservoir Sakura Finetek Japan Cat# 6186-4P Mouse CCL3/MIP-1 alpha ELISA Kit - Quantikine R&D Systems Cat# |