Multiple Displacement Amplification:Article Title: MEK interactions tune RAF kinase sensitivity to conformation-selective inhibition.
Article Snippet: .. All cancer cell lines used in this study, namely Mia PaCa-2 (CRL-1420), MDA-MB-453 (HTB-131), SW-480 (CCL-228), DLD-1 (CCL-221), MDA-MB-231 (HTB-26), HCT-116 (CCL-247), LoVo (CCL-229), HPAF-II (CRL-1997), AsPc-1 (CRL-1682), A549 (CCL-185) and Calu-6 (HTB56) were purchased from the American Type Culture collection (ATCC) and cultured in DMEM (Thermo Fisher) + 10% FBS (Millipore Sigma), with the exception of HCT-116 cells, which were cultured in McCoy’s medium (Thermo Fisher) supplemented with 10% FBS. .. All cell lines were authenticated by ATCC through short tandem repeat profiling.
Cell Culture:Article Title: MEK interactions tune RAF kinase sensitivity to conformation-selective inhibition.
Article Snippet: .. All cancer cell lines used in this study, namely Mia PaCa-2 (CRL-1420), MDA-MB-453 (HTB-131), SW-480 (CCL-228), DLD-1 (CCL-221), MDA-MB-231 (HTB-26), HCT-116 (CCL-247), LoVo (CCL-229), HPAF-II (CRL-1997), AsPc-1 (CRL-1682), A549 (CCL-185) and Calu-6 (HTB56) were purchased from the American Type Culture collection (ATCC) and cultured in DMEM (Thermo Fisher) + 10% FBS (Millipore Sigma), with the exception of HCT-116 cells, which were cultured in McCoy’s medium (Thermo Fisher) supplemented with 10% FBS. .. All cell lines were authenticated by ATCC through short tandem repeat profiling.
Article Title: Discovery and Development of a Potent LIMK2 Isoform-Specific Degrader
Article Snippet: Subsequently, EGFP-YTHDF2-P2A-mCherry was cloned into the lentiviral vector pCW57-MCS1-P2A-MCS2 (Hygro) (Addgene plasmid #80922) to generate pCW57-HygB-EGFP-LIMK2-P2A-mCherry. pCW57-MCS1-P2A-MCS2 (Hygro) was a gift from Adam Karpf. .. HEK293T cells (ATCC, CRL-3216) were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HUCCT1 cells (ATCC, CRL-1997) were cultured in RPMI medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin (P/S) antibiotic mix (all from Sigma-Aldrich, St. Louis, Missouri, USA). ..
Plasmid Preparation:Article Title: SLC22A3/OCT3 drives serotonin-mediated stemness in pancreatic cancer.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER RNase A ThermoFisher Cat. No. # EN0531 Dispase ThermoFisher Cat. No.# 17105041 FFN246 MedChem Express Cat. No.# 2210244-83-8 Decynium-22 Sigma Cat. No.# 323764 Hoechst 33342 Invitrogen Cat. No.# 62249 Verapamil Sigma Cat. No.#V4629-5G B27 Invitrogen Cat# 17504-044 EGF (20 ng/mL) Fujifilm Biosciences Cat. No # 100-26 bFGF (10ng/mL) R&D Systems Cat. No # 3718-FB-100 Insulin (5–10 μg/mL) Gibco Cat. No # 12-585-014 BCIP/NBT Invitrogen Cat. No # WP20001 Critical commercial assays BCA Protein Quantification Kit Thermo Fisher Scientific Cat. No # 23227 RNeasy Mini Kit Qiagen Cat. No # 74104 NucleoBond Plasmid Midiprep Kit Macherey-Nagel Cat. No # 740410.50 Experimental models: Cell lines HPNE Dr. Michel Ouellette N/A SUIT-2 Dr. Michel Ouellette N/A CD18/HPAF-II ATCC Cat. No.# CRL-1997 MIAPACA2 ATCC Cat. No.# CRL-1420 SW1990 ATCC Cat. No.# CRL-2172 HPNE/E6/E7 Dr. Michel Ouellette N/A HPNE/E6/E7/ST Dr. Michel Ouellette N/A HPNE/E6/E7/KRAS Dr. Michel Ouellette N/A HPNE/E6/E7/ST/KRAS Dr. Michel Ouellette N/A HPDE Dr. Michel Ouellette N/A HPDE/KRAS Dr. Michel Ouellette N/A HEK293T Gift from Ashu Shah N/A Deposited data mRNA profiles for hESC-derived cells and primary cells GEO (NCBI) GEO: GSE97987 RNA sequencing of circulating tumor cells implicates WNT signaling in pancreatic cancer metastasis GEO (NCBI) GEO: GSE40174 Pancreatic Adenocarcinoma TCGA-PAAD TCGA-PAAD Whole-Tissue Gene Expression Study of Pancreatic Ductal Adenocarcinoma GEO (NCBI) GEO: GSE15471 Expression data from Mayo Clinic Pancreatic Tumor and Normal samples GEO (NCBI) GEO: GSE16515 Affymetrix Arrays Interrogated with Tumor/Normal Pancreatic Samples GEO (NCBI) GEO: GSE22780 Multimodal Mapping of the Tumor and Peripheral Blood Immune Landscape in Human Pancreatic Cancer GEO (NCBI) GEO: GSE155698 Single-cell RNA-seq highlights intra-tumoral heterogeneity and malignant progression in pancreatic ductal adenocarcinoma Genome Sequence Archive (GSA) GSA: CRA001160 Identification and characterization of new pancreatic cancer stem cells marker GEO (NCBI) GEO: GSE172303 Software and algorithms ImageJ Fiji https://imagej.nih.gov/ij/ GraphPad Prism GraphPad Software Home - GraphPad QuantaSoft BioRad QuantaSoft Software QuantaSoftTM Software (Continued on next page) 20 Cell Reports 45, 117387, June 23, 2026 ..
RNA Sequencing:Article Title: SLC22A3/OCT3 drives serotonin-mediated stemness in pancreatic cancer.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER RNase A ThermoFisher Cat. No. # EN0531 Dispase ThermoFisher Cat. No.# 17105041 FFN246 MedChem Express Cat. No.# 2210244-83-8 Decynium-22 Sigma Cat. No.# 323764 Hoechst 33342 Invitrogen Cat. No.# 62249 Verapamil Sigma Cat. No.#V4629-5G B27 Invitrogen Cat# 17504-044 EGF (20 ng/mL) Fujifilm Biosciences Cat. No # 100-26 bFGF (10ng/mL) R&D Systems Cat. No # 3718-FB-100 Insulin (5–10 μg/mL) Gibco Cat. No # 12-585-014 BCIP/NBT Invitrogen Cat. No # WP20001 Critical commercial assays BCA Protein Quantification Kit Thermo Fisher Scientific Cat. No # 23227 RNeasy Mini Kit Qiagen Cat. No # 74104 NucleoBond Plasmid Midiprep Kit Macherey-Nagel Cat. No # 740410.50 Experimental models: Cell lines HPNE Dr. Michel Ouellette N/A SUIT-2 Dr. Michel Ouellette N/A CD18/HPAF-II ATCC Cat. No.# CRL-1997 MIAPACA2 ATCC Cat. No.# CRL-1420 SW1990 ATCC Cat. No.# CRL-2172 HPNE/E6/E7 Dr. Michel Ouellette N/A HPNE/E6/E7/ST Dr. Michel Ouellette N/A HPNE/E6/E7/KRAS Dr. Michel Ouellette N/A HPNE/E6/E7/ST/KRAS Dr. Michel Ouellette N/A HPDE Dr. Michel Ouellette N/A HPDE/KRAS Dr. Michel Ouellette N/A HEK293T Gift from Ashu Shah N/A Deposited data mRNA profiles for hESC-derived cells and primary cells GEO (NCBI) GEO: GSE97987 RNA sequencing of circulating tumor cells implicates WNT signaling in pancreatic cancer metastasis GEO (NCBI) GEO: GSE40174 Pancreatic Adenocarcinoma TCGA-PAAD TCGA-PAAD Whole-Tissue Gene Expression Study of Pancreatic Ductal Adenocarcinoma GEO (NCBI) GEO: GSE15471 Expression data from Mayo Clinic Pancreatic Tumor and Normal samples GEO (NCBI) GEO: GSE16515 Affymetrix Arrays Interrogated with Tumor/Normal Pancreatic Samples GEO (NCBI) GEO: GSE22780 Multimodal Mapping of the Tumor and Peripheral Blood Immune Landscape in Human Pancreatic Cancer GEO (NCBI) GEO: GSE155698 Single-cell RNA-seq highlights intra-tumoral heterogeneity and malignant progression in pancreatic ductal adenocarcinoma Genome Sequence Archive (GSA) GSA: CRA001160 Identification and characterization of new pancreatic cancer stem cells marker GEO (NCBI) GEO: GSE172303 Software and algorithms ImageJ Fiji https://imagej.nih.gov/ij/ GraphPad Prism GraphPad Software Home - GraphPad QuantaSoft BioRad QuantaSoft Software QuantaSoftTM Software (Continued on next page) 20 Cell Reports 45, 117387, June 23, 2026 ..
Gene Expression:Article Title: SLC22A3/OCT3 drives serotonin-mediated stemness in pancreatic cancer.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER RNase A ThermoFisher Cat. No. # EN0531 Dispase ThermoFisher Cat. No.# 17105041 FFN246 MedChem Express Cat. No.# 2210244-83-8 Decynium-22 Sigma Cat. No.# 323764 Hoechst 33342 Invitrogen Cat. No.# 62249 Verapamil Sigma Cat. No.#V4629-5G B27 Invitrogen Cat# 17504-044 EGF (20 ng/mL) Fujifilm Biosciences Cat. No # 100-26 bFGF (10ng/mL) R&D Systems Cat. No # 3718-FB-100 Insulin (5–10 μg/mL) Gibco Cat. No # 12-585-014 BCIP/NBT Invitrogen Cat. No # WP20001 Critical commercial assays BCA Protein Quantification Kit Thermo Fisher Scientific Cat. No # 23227 RNeasy Mini Kit Qiagen Cat. No # 74104 NucleoBond Plasmid Midiprep Kit Macherey-Nagel Cat. No # 740410.50 Experimental models: Cell lines HPNE Dr. Michel Ouellette N/A SUIT-2 Dr. Michel Ouellette N/A CD18/HPAF-II ATCC Cat. No.# CRL-1997 MIAPACA2 ATCC Cat. No.# CRL-1420 SW1990 ATCC Cat. No.# CRL-2172 HPNE/E6/E7 Dr. Michel Ouellette N/A HPNE/E6/E7/ST Dr. Michel Ouellette N/A HPNE/E6/E7/KRAS Dr. Michel Ouellette N/A HPNE/E6/E7/ST/KRAS Dr. Michel Ouellette N/A HPDE Dr. Michel Ouellette N/A HPDE/KRAS Dr. Michel Ouellette N/A HEK293T Gift from Ashu Shah N/A Deposited data mRNA profiles for hESC-derived cells and primary cells GEO (NCBI) GEO: GSE97987 RNA sequencing of circulating tumor cells implicates WNT signaling in pancreatic cancer metastasis GEO (NCBI) GEO: GSE40174 Pancreatic Adenocarcinoma TCGA-PAAD TCGA-PAAD Whole-Tissue Gene Expression Study of Pancreatic Ductal Adenocarcinoma GEO (NCBI) GEO: GSE15471 Expression data from Mayo Clinic Pancreatic Tumor and Normal samples GEO (NCBI) GEO: GSE16515 Affymetrix Arrays Interrogated with Tumor/Normal Pancreatic Samples GEO (NCBI) GEO: GSE22780 Multimodal Mapping of the Tumor and Peripheral Blood Immune Landscape in Human Pancreatic Cancer GEO (NCBI) GEO: GSE155698 Single-cell RNA-seq highlights intra-tumoral heterogeneity and malignant progression in pancreatic ductal adenocarcinoma Genome Sequence Archive (GSA) GSA: CRA001160 Identification and characterization of new pancreatic cancer stem cells marker GEO (NCBI) GEO: GSE172303 Software and algorithms ImageJ Fiji https://imagej.nih.gov/ij/ GraphPad Prism GraphPad Software Home - GraphPad QuantaSoft BioRad QuantaSoft Software QuantaSoftTM Software (Continued on next page) 20 Cell Reports 45, 117387, June 23, 2026 ..
Expressing:Article Title: SLC22A3/OCT3 drives serotonin-mediated stemness in pancreatic cancer.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER RNase A ThermoFisher Cat. No. # EN0531 Dispase ThermoFisher Cat. No.# 17105041 FFN246 MedChem Express Cat. No.# 2210244-83-8 Decynium-22 Sigma Cat. No.# 323764 Hoechst 33342 Invitrogen Cat. No.# 62249 Verapamil Sigma Cat. No.#V4629-5G B27 Invitrogen Cat# 17504-044 EGF (20 ng/mL) Fujifilm Biosciences Cat. No # 100-26 bFGF (10ng/mL) R&D Systems Cat. No # 3718-FB-100 Insulin (5–10 μg/mL) Gibco Cat. No # 12-585-014 BCIP/NBT Invitrogen Cat. No # WP20001 Critical commercial assays BCA Protein Quantification Kit Thermo Fisher Scientific Cat. No # 23227 RNeasy Mini Kit Qiagen Cat. No # 74104 NucleoBond Plasmid Midiprep Kit Macherey-Nagel Cat. No # 740410.50 Experimental models: Cell lines HPNE Dr. Michel Ouellette N/A SUIT-2 Dr. Michel Ouellette N/A CD18/HPAF-II ATCC Cat. No.# CRL-1997 MIAPACA2 ATCC Cat. No.# CRL-1420 SW1990 ATCC Cat. No.# CRL-2172 HPNE/E6/E7 Dr. Michel Ouellette N/A HPNE/E6/E7/ST Dr. Michel Ouellette N/A HPNE/E6/E7/KRAS Dr. Michel Ouellette N/A HPNE/E6/E7/ST/KRAS Dr. Michel Ouellette N/A HPDE Dr. Michel Ouellette N/A HPDE/KRAS Dr. Michel Ouellette N/A HEK293T Gift from Ashu Shah N/A Deposited data mRNA profiles for hESC-derived cells and primary cells GEO (NCBI) GEO: GSE97987 RNA sequencing of circulating tumor cells implicates WNT signaling in pancreatic cancer metastasis GEO (NCBI) GEO: GSE40174 Pancreatic Adenocarcinoma TCGA-PAAD TCGA-PAAD Whole-Tissue Gene Expression Study of Pancreatic Ductal Adenocarcinoma GEO (NCBI) GEO: GSE15471 Expression data from Mayo Clinic Pancreatic Tumor and Normal samples GEO (NCBI) GEO: GSE16515 Affymetrix Arrays Interrogated with Tumor/Normal Pancreatic Samples GEO (NCBI) GEO: GSE22780 Multimodal Mapping of the Tumor and Peripheral Blood Immune Landscape in Human Pancreatic Cancer GEO (NCBI) GEO: GSE155698 Single-cell RNA-seq highlights intra-tumoral heterogeneity and malignant progression in pancreatic ductal adenocarcinoma Genome Sequence Archive (GSA) GSA: CRA001160 Identification and characterization of new pancreatic cancer stem cells marker GEO (NCBI) GEO: GSE172303 Software and algorithms ImageJ Fiji https://imagej.nih.gov/ij/ GraphPad Prism GraphPad Software Home - GraphPad QuantaSoft BioRad QuantaSoft Software QuantaSoftTM Software (Continued on next page) 20 Cell Reports 45, 117387, June 23, 2026 ..
Single Cell:Article Title: SLC22A3/OCT3 drives serotonin-mediated stemness in pancreatic cancer.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER RNase A ThermoFisher Cat. No. # EN0531 Dispase ThermoFisher Cat. No.# 17105041 FFN246 MedChem Express Cat. No.# 2210244-83-8 Decynium-22 Sigma Cat. No.# 323764 Hoechst 33342 Invitrogen Cat. No.# 62249 Verapamil Sigma Cat. No.#V4629-5G B27 Invitrogen Cat# 17504-044 EGF (20 ng/mL) Fujifilm Biosciences Cat. No # 100-26 bFGF (10ng/mL) R&D Systems Cat. No # 3718-FB-100 Insulin (5–10 μg/mL) Gibco Cat. No # 12-585-014 BCIP/NBT Invitrogen Cat. No # WP20001 Critical commercial assays BCA Protein Quantification Kit Thermo Fisher Scientific Cat. No # 23227 RNeasy Mini Kit Qiagen Cat. No # 74104 NucleoBond Plasmid Midiprep Kit Macherey-Nagel Cat. No # 740410.50 Experimental models: Cell lines HPNE Dr. Michel Ouellette N/A SUIT-2 Dr. Michel Ouellette N/A CD18/HPAF-II ATCC Cat. No.# CRL-1997 MIAPACA2 ATCC Cat. No.# CRL-1420 SW1990 ATCC Cat. No.# CRL-2172 HPNE/E6/E7 Dr. Michel Ouellette N/A HPNE/E6/E7/ST Dr. Michel Ouellette N/A HPNE/E6/E7/KRAS Dr. Michel Ouellette N/A HPNE/E6/E7/ST/KRAS Dr. Michel Ouellette N/A HPDE Dr. Michel Ouellette N/A HPDE/KRAS Dr. Michel Ouellette N/A HEK293T Gift from Ashu Shah N/A Deposited data mRNA profiles for hESC-derived cells and primary cells GEO (NCBI) GEO: GSE97987 RNA sequencing of circulating tumor cells implicates WNT signaling in pancreatic cancer metastasis GEO (NCBI) GEO: GSE40174 Pancreatic Adenocarcinoma TCGA-PAAD TCGA-PAAD Whole-Tissue Gene Expression Study of Pancreatic Ductal Adenocarcinoma GEO (NCBI) GEO: GSE15471 Expression data from Mayo Clinic Pancreatic Tumor and Normal samples GEO (NCBI) GEO: GSE16515 Affymetrix Arrays Interrogated with Tumor/Normal Pancreatic Samples GEO (NCBI) GEO: GSE22780 Multimodal Mapping of the Tumor and Peripheral Blood Immune Landscape in Human Pancreatic Cancer GEO (NCBI) GEO: GSE155698 Single-cell RNA-seq highlights intra-tumoral heterogeneity and malignant progression in pancreatic ductal adenocarcinoma Genome Sequence Archive (GSA) GSA: CRA001160 Identification and characterization of new pancreatic cancer stem cells marker GEO (NCBI) GEO: GSE172303 Software and algorithms ImageJ Fiji https://imagej.nih.gov/ij/ GraphPad Prism GraphPad Software Home - GraphPad QuantaSoft BioRad QuantaSoft Software QuantaSoftTM Software (Continued on next page) 20 Cell Reports 45, 117387, June 23, 2026 ..
Sequencing:Article Title: SLC22A3/OCT3 drives serotonin-mediated stemness in pancreatic cancer.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER RNase A ThermoFisher Cat. No. # EN0531 Dispase ThermoFisher Cat. No.# 17105041 FFN246 MedChem Express Cat. No.# 2210244-83-8 Decynium-22 Sigma Cat. No.# 323764 Hoechst 33342 Invitrogen Cat. No.# 62249 Verapamil Sigma Cat. No.#V4629-5G B27 Invitrogen Cat# 17504-044 EGF (20 ng/mL) Fujifilm Biosciences Cat. No # 100-26 bFGF (10ng/mL) R&D Systems Cat. No # 3718-FB-100 Insulin (5–10 μg/mL) Gibco Cat. No # 12-585-014 BCIP/NBT Invitrogen Cat. No # WP20001 Critical commercial assays BCA Protein Quantification Kit Thermo Fisher Scientific Cat. No # 23227 RNeasy Mini Kit Qiagen Cat. No # 74104 NucleoBond Plasmid Midiprep Kit Macherey-Nagel Cat. No # 740410.50 Experimental models: Cell lines HPNE Dr. Michel Ouellette N/A SUIT-2 Dr. Michel Ouellette N/A CD18/HPAF-II ATCC Cat. No.# CRL-1997 MIAPACA2 ATCC Cat. No.# CRL-1420 SW1990 ATCC Cat. No.# CRL-2172 HPNE/E6/E7 Dr. Michel Ouellette N/A HPNE/E6/E7/ST Dr. Michel Ouellette N/A HPNE/E6/E7/KRAS Dr. Michel Ouellette N/A HPNE/E6/E7/ST/KRAS Dr. Michel Ouellette N/A HPDE Dr. Michel Ouellette N/A HPDE/KRAS Dr. Michel Ouellette N/A HEK293T Gift from Ashu Shah N/A Deposited data mRNA profiles for hESC-derived cells and primary cells GEO (NCBI) GEO: GSE97987 RNA sequencing of circulating tumor cells implicates WNT signaling in pancreatic cancer metastasis GEO (NCBI) GEO: GSE40174 Pancreatic Adenocarcinoma TCGA-PAAD TCGA-PAAD Whole-Tissue Gene Expression Study of Pancreatic Ductal Adenocarcinoma GEO (NCBI) GEO: GSE15471 Expression data from Mayo Clinic Pancreatic Tumor and Normal samples GEO (NCBI) GEO: GSE16515 Affymetrix Arrays Interrogated with Tumor/Normal Pancreatic Samples GEO (NCBI) GEO: GSE22780 Multimodal Mapping of the Tumor and Peripheral Blood Immune Landscape in Human Pancreatic Cancer GEO (NCBI) GEO: GSE155698 Single-cell RNA-seq highlights intra-tumoral heterogeneity and malignant progression in pancreatic ductal adenocarcinoma Genome Sequence Archive (GSA) GSA: CRA001160 Identification and characterization of new pancreatic cancer stem cells marker GEO (NCBI) GEO: GSE172303 Software and algorithms ImageJ Fiji https://imagej.nih.gov/ij/ GraphPad Prism GraphPad Software Home - GraphPad QuantaSoft BioRad QuantaSoft Software QuantaSoftTM Software (Continued on next page) 20 Cell Reports 45, 117387, June 23, 2026 ..
Marker:Article Title: SLC22A3/OCT3 drives serotonin-mediated stemness in pancreatic cancer.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER RNase A ThermoFisher Cat. No. # EN0531 Dispase ThermoFisher Cat. No.# 17105041 FFN246 MedChem Express Cat. No.# 2210244-83-8 Decynium-22 Sigma Cat. No.# 323764 Hoechst 33342 Invitrogen Cat. No.# 62249 Verapamil Sigma Cat. No.#V4629-5G B27 Invitrogen Cat# 17504-044 EGF (20 ng/mL) Fujifilm Biosciences Cat. No # 100-26 bFGF (10ng/mL) R&D Systems Cat. No # 3718-FB-100 Insulin (5–10 μg/mL) Gibco Cat. No # 12-585-014 BCIP/NBT Invitrogen Cat. No # WP20001 Critical commercial assays BCA Protein Quantification Kit Thermo Fisher Scientific Cat. No # 23227 RNeasy Mini Kit Qiagen Cat. No # 74104 NucleoBond Plasmid Midiprep Kit Macherey-Nagel Cat. No # 740410.50 Experimental models: Cell lines HPNE Dr. Michel Ouellette N/A SUIT-2 Dr. Michel Ouellette N/A CD18/HPAF-II ATCC Cat. No.# CRL-1997 MIAPACA2 ATCC Cat. No.# CRL-1420 SW1990 ATCC Cat. No.# CRL-2172 HPNE/E6/E7 Dr. Michel Ouellette N/A HPNE/E6/E7/ST Dr. Michel Ouellette N/A HPNE/E6/E7/KRAS Dr. Michel Ouellette N/A HPNE/E6/E7/ST/KRAS Dr. Michel Ouellette N/A HPDE Dr. Michel Ouellette N/A HPDE/KRAS Dr. Michel Ouellette N/A HEK293T Gift from Ashu Shah N/A Deposited data mRNA profiles for hESC-derived cells and primary cells GEO (NCBI) GEO: GSE97987 RNA sequencing of circulating tumor cells implicates WNT signaling in pancreatic cancer metastasis GEO (NCBI) GEO: GSE40174 Pancreatic Adenocarcinoma TCGA-PAAD TCGA-PAAD Whole-Tissue Gene Expression Study of Pancreatic Ductal Adenocarcinoma GEO (NCBI) GEO: GSE15471 Expression data from Mayo Clinic Pancreatic Tumor and Normal samples GEO (NCBI) GEO: GSE16515 Affymetrix Arrays Interrogated with Tumor/Normal Pancreatic Samples GEO (NCBI) GEO: GSE22780 Multimodal Mapping of the Tumor and Peripheral Blood Immune Landscape in Human Pancreatic Cancer GEO (NCBI) GEO: GSE155698 Single-cell RNA-seq highlights intra-tumoral heterogeneity and malignant progression in pancreatic ductal adenocarcinoma Genome Sequence Archive (GSA) GSA: CRA001160 Identification and characterization of new pancreatic cancer stem cells marker GEO (NCBI) GEO: GSE172303 Software and algorithms ImageJ Fiji https://imagej.nih.gov/ij/ GraphPad Prism GraphPad Software Home - GraphPad QuantaSoft BioRad QuantaSoft Software QuantaSoftTM Software (Continued on next page) 20 Cell Reports 45, 117387, June 23, 2026 ..
Software:Article Title: SLC22A3/OCT3 drives serotonin-mediated stemness in pancreatic cancer.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER RNase A ThermoFisher Cat. No. # EN0531 Dispase ThermoFisher Cat. No.# 17105041 FFN246 MedChem Express Cat. No.# 2210244-83-8 Decynium-22 Sigma Cat. No.# 323764 Hoechst 33342 Invitrogen Cat. No.# 62249 Verapamil Sigma Cat. No.#V4629-5G B27 Invitrogen Cat# 17504-044 EGF (20 ng/mL) Fujifilm Biosciences Cat. No # 100-26 bFGF (10ng/mL) R&D Systems Cat. No # 3718-FB-100 Insulin (5–10 μg/mL) Gibco Cat. No # 12-585-014 BCIP/NBT Invitrogen Cat. No # WP20001 Critical commercial assays BCA Protein Quantification Kit Thermo Fisher Scientific Cat. No # 23227 RNeasy Mini Kit Qiagen Cat. No # 74104 NucleoBond Plasmid Midiprep Kit Macherey-Nagel Cat. No # 740410.50 Experimental models: Cell lines HPNE Dr. Michel Ouellette N/A SUIT-2 Dr. Michel Ouellette N/A CD18/HPAF-II ATCC Cat. No.# CRL-1997 MIAPACA2 ATCC Cat. No.# CRL-1420 SW1990 ATCC Cat. No.# CRL-2172 HPNE/E6/E7 Dr. Michel Ouellette N/A HPNE/E6/E7/ST Dr. Michel Ouellette N/A HPNE/E6/E7/KRAS Dr. Michel Ouellette N/A HPNE/E6/E7/ST/KRAS Dr. Michel Ouellette N/A HPDE Dr. Michel Ouellette N/A HPDE/KRAS Dr. Michel Ouellette N/A HEK293T Gift from Ashu Shah N/A Deposited data mRNA profiles for hESC-derived cells and primary cells GEO (NCBI) GEO: GSE97987 RNA sequencing of circulating tumor cells implicates WNT signaling in pancreatic cancer metastasis GEO (NCBI) GEO: GSE40174 Pancreatic Adenocarcinoma TCGA-PAAD TCGA-PAAD Whole-Tissue Gene Expression Study of Pancreatic Ductal Adenocarcinoma GEO (NCBI) GEO: GSE15471 Expression data from Mayo Clinic Pancreatic Tumor and Normal samples GEO (NCBI) GEO: GSE16515 Affymetrix Arrays Interrogated with Tumor/Normal Pancreatic Samples GEO (NCBI) GEO: GSE22780 Multimodal Mapping of the Tumor and Peripheral Blood Immune Landscape in Human Pancreatic Cancer GEO (NCBI) GEO: GSE155698 Single-cell RNA-seq highlights intra-tumoral heterogeneity and malignant progression in pancreatic ductal adenocarcinoma Genome Sequence Archive (GSA) GSA: CRA001160 Identification and characterization of new pancreatic cancer stem cells marker GEO (NCBI) GEO: GSE172303 Software and algorithms ImageJ Fiji https://imagej.nih.gov/ij/ GraphPad Prism GraphPad Software Home - GraphPad QuantaSoft BioRad QuantaSoft Software QuantaSoftTM Software (Continued on next page) 20 Cell Reports 45, 117387, June 23, 2026 ..
Modification:Article Title: Discovery and Development of a Potent LIMK2 Isoform-Specific Degrader
Article Snippet: Subsequently, EGFP-YTHDF2-P2A-mCherry was cloned into the lentiviral vector pCW57-MCS1-P2A-MCS2 (Hygro) (Addgene plasmid #80922) to generate pCW57-HygB-EGFP-LIMK2-P2A-mCherry. pCW57-MCS1-P2A-MCS2 (Hygro) was a gift from Adam Karpf. .. HEK293T cells (ATCC, CRL-3216) were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), while HUCCT1 cells (ATCC, CRL-1997) were cultured in RPMI medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin (P/S) antibiotic mix (all from Sigma-Aldrich, St. Louis, Missouri, USA). ..
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