Flow Cytometry:Article Title: ALDH1A3 promotes the lung metastatic organotropism of pancreatic ductal adenocarcinoma through semaphorin signaling.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Magna ChIP A/G Chromatin Immunoprecipitation Kit Merck Cat# 17-10085 Nuclear and cytoplasmic protein extraction kit Beyotime Cat# P0027 Deposited data RNA Sequencing data This paper GEOID: GSE248375 RNA Sequencing data This paper HRA011000 Multi-omic profiling of lung and liver tumor microenvironments of metastatic pancreatic cancer reveals site-specific immuneregulatory features NCBI Gene Expression Omnibus GEOID: GSE172104 Experimental models: Cell lines Mouse PDAC cell lines: 399, 403, 445,897,907,908,928, 952 and 5M409L p48cre; KrasG12D/+; Tsc1fl/+ mice N/A Mouse PDAC cell lines 5M409L p48cre; KrasG12D/+; p53 fl/fl mice N/A Mouse LPCs This paper N/A HPAC ATCC Cat# CRL-2119; RRID: CVCL_3517 AsPC-1 ATCC Cat# CRL-1682; RRID: CVCL_0152 MLE-12 ATCC Cat# CRL-2110, RRID:CVCL_3751 Experimental models: Organisms/strains C57BL/6J Charles River Strain #213 BALB/c Nude Charles River Strain #401 Oligonucleotides Primers for real-time PCR analysis, see Supplemental information This paper N/A Primers for CHIP PCR analysis, see Supplemental information This paper N/A Human Aldh1a3 shRNA, see Supplemental information This paper N/A Mouse Plxnb1 shRNA, see Supplemental information This paper N/A Mouse Nrp1 shRNA, see Supplemental information This paper N/A Mouse Sema3a siRNA, see Supplemental information This paper N/A Recombinant DNA Mouse Aldh1a3 Plasmid Origene Cat# MG222097 Mouse Plxnb1 Plasmid Origene Cat# MR222673 Mouse Nrp1 Plasmid Origene Cat# MR211197 pCMV6-Entry Plasmid Origene Cat# PS100001 Mouse Aldh1a3 CRISPR Plasmid Santa Cruz Biotechnology Cat# sc-425322 Software and algorithms GraphPad Prism software GraphPad Software https://www.graphpad.com/ Bowtie2 SourceForge https://bowtie-bio.sourceforge.net/bowtie2 HISAT Center for Computational Biology at Johns Hopkins University https://ccb.jhu.edu/software/hisat ImageJ NIH Image https://imagej.net/imagej-wiki-static/ImageJ DESeq2 Bioconductor https://bioconductor.org/packages/release/ bioc/html/DESeq2.html R statistical software The R Project https://www.r-project.org (Continued on next page) e2 iScience 29, 115950, June 19, 2026
Analysis:Article Title: ALDH1A3 promotes the lung metastatic organotropism of pancreatic ductal adenocarcinoma through semaphorin signaling.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Magna ChIP A/G Chromatin Immunoprecipitation Kit Merck Cat# 17-10085 Nuclear and cytoplasmic protein extraction kit Beyotime Cat# P0027 Deposited data RNA Sequencing data This paper GEOID: GSE248375 RNA Sequencing data This paper HRA011000 Multi-omic profiling of lung and liver tumor microenvironments of metastatic pancreatic cancer reveals site-specific immuneregulatory features NCBI Gene Expression Omnibus GEOID: GSE172104 Experimental models: Cell lines Mouse PDAC cell lines: 399, 403, 445,897,907,908,928, 952 and 5M409L p48cre; KrasG12D/+; Tsc1fl/+ mice N/A Mouse PDAC cell lines 5M409L p48cre; KrasG12D/+; p53 fl/fl mice N/A Mouse LPCs This paper N/A HPAC ATCC Cat# CRL-2119; RRID: CVCL_3517 AsPC-1 ATCC Cat# CRL-1682; RRID: CVCL_0152 MLE-12 ATCC Cat# CRL-2110, RRID:CVCL_3751 Experimental models: Organisms/strains C57BL/6J Charles River Strain #213 BALB/c Nude Charles River Strain #401 Oligonucleotides Primers for real-time PCR analysis, see Supplemental information This paper N/A Primers for CHIP PCR analysis, see Supplemental information This paper N/A Human Aldh1a3 shRNA, see Supplemental information This paper N/A Mouse Plxnb1 shRNA, see Supplemental information This paper N/A Mouse Nrp1 shRNA, see Supplemental information This paper N/A Mouse Sema3a siRNA, see Supplemental information This paper N/A Recombinant DNA Mouse Aldh1a3 Plasmid Origene Cat# MG222097 Mouse Plxnb1 Plasmid Origene Cat# MR222673 Mouse Nrp1 Plasmid Origene Cat# MR211197 pCMV6-Entry Plasmid Origene Cat# PS100001 Mouse Aldh1a3 CRISPR Plasmid Santa Cruz Biotechnology Cat# sc-425322 Software and algorithms GraphPad Prism software GraphPad Software https://www.graphpad.com/ Bowtie2 SourceForge https://bowtie-bio.sourceforge.net/bowtie2 HISAT Center for Computational Biology at Johns Hopkins University https://ccb.jhu.edu/software/hisat ImageJ NIH Image https://imagej.net/imagej-wiki-static/ImageJ DESeq2 Bioconductor https://bioconductor.org/packages/release/ bioc/html/DESeq2.html R statistical software The R Project https://www.r-project.org (Continued on next page) e2 iScience 29, 115950, June 19, 2026
Expressing:Article Title: ALDH1A3 promotes the lung metastatic organotropism of pancreatic ductal adenocarcinoma through semaphorin signaling.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Magna ChIP A/G Chromatin Immunoprecipitation Kit Merck Cat# 17-10085 Nuclear and cytoplasmic protein extraction kit Beyotime Cat# P0027 Deposited data RNA Sequencing data This paper GEOID: GSE248375 RNA Sequencing data This paper HRA011000 Multi-omic profiling of lung and liver tumor microenvironments of metastatic pancreatic cancer reveals site-specific immuneregulatory features NCBI Gene Expression Omnibus GEOID: GSE172104 Experimental models: Cell lines Mouse PDAC cell lines: 399, 403, 445,897,907,908,928, 952 and 5M409L p48cre; KrasG12D/+; Tsc1fl/+ mice N/A Mouse PDAC cell lines 5M409L p48cre; KrasG12D/+; p53 fl/fl mice N/A Mouse LPCs This paper N/A HPAC ATCC Cat# CRL-2119; RRID: CVCL_3517 AsPC-1 ATCC Cat# CRL-1682; RRID: CVCL_0152 MLE-12 ATCC Cat# CRL-2110, RRID:CVCL_3751 Experimental models: Organisms/strains C57BL/6J Charles River Strain #213 BALB/c Nude Charles River Strain #401 Oligonucleotides Primers for real-time PCR analysis, see Supplemental information This paper N/A Primers for CHIP PCR analysis, see Supplemental information This paper N/A Human Aldh1a3 shRNA, see Supplemental information This paper N/A Mouse Plxnb1 shRNA, see Supplemental information This paper N/A Mouse Nrp1 shRNA, see Supplemental information This paper N/A Mouse Sema3a siRNA, see Supplemental information This paper N/A Recombinant DNA Mouse Aldh1a3 Plasmid Origene Cat# MG222097 Mouse Plxnb1 Plasmid Origene Cat# MR222673 Mouse Nrp1 Plasmid Origene Cat# MR211197 pCMV6-Entry Plasmid Origene Cat# PS100001 Mouse Aldh1a3 CRISPR Plasmid Santa Cruz Biotechnology Cat# sc-425322 Software and algorithms GraphPad Prism software GraphPad Software https://www.graphpad.com/ Bowtie2 SourceForge https://bowtie-bio.sourceforge.net/bowtie2 HISAT Center for Computational Biology at Johns Hopkins University https://ccb.jhu.edu/software/hisat ImageJ NIH Image https://imagej.net/imagej-wiki-static/ImageJ DESeq2 Bioconductor https://bioconductor.org/packages/release/ bioc/html/DESeq2.html R statistical software The R Project https://www.r-project.org (Continued on next page) e2 iScience 29, 115950, June 19, 2026
Fluorescence:Article Title: ALDH1A3 promotes the lung metastatic organotropism of pancreatic ductal adenocarcinoma through semaphorin signaling.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Magna ChIP A/G Chromatin Immunoprecipitation Kit Merck Cat# 17-10085 Nuclear and cytoplasmic protein extraction kit Beyotime Cat# P0027 Deposited data RNA Sequencing data This paper GEOID: GSE248375 RNA Sequencing data This paper HRA011000 Multi-omic profiling of lung and liver tumor microenvironments of metastatic pancreatic cancer reveals site-specific immuneregulatory features NCBI Gene Expression Omnibus GEOID: GSE172104 Experimental models: Cell lines Mouse PDAC cell lines: 399, 403, 445,897,907,908,928, 952 and 5M409L p48cre; KrasG12D/+; Tsc1fl/+ mice N/A Mouse PDAC cell lines 5M409L p48cre; KrasG12D/+; p53 fl/fl mice N/A Mouse LPCs This paper N/A HPAC ATCC Cat# CRL-2119; RRID: CVCL_3517 AsPC-1 ATCC Cat# CRL-1682; RRID: CVCL_0152 MLE-12 ATCC Cat# CRL-2110, RRID:CVCL_3751 Experimental models: Organisms/strains C57BL/6J Charles River Strain #213 BALB/c Nude Charles River Strain #401 Oligonucleotides Primers for real-time PCR analysis, see Supplemental information This paper N/A Primers for CHIP PCR analysis, see Supplemental information This paper N/A Human Aldh1a3 shRNA, see Supplemental information This paper N/A Mouse Plxnb1 shRNA, see Supplemental information This paper N/A Mouse Nrp1 shRNA, see Supplemental information This paper N/A Mouse Sema3a siRNA, see Supplemental information This paper N/A Recombinant DNA Mouse Aldh1a3 Plasmid Origene Cat# MG222097 Mouse Plxnb1 Plasmid Origene Cat# MR222673 Mouse Nrp1 Plasmid Origene Cat# MR211197 pCMV6-Entry Plasmid Origene Cat# PS100001 Mouse Aldh1a3 CRISPR Plasmid Santa Cruz Biotechnology Cat# sc-425322 Software and algorithms GraphPad Prism software GraphPad Software https://www.graphpad.com/ Bowtie2 SourceForge https://bowtie-bio.sourceforge.net/bowtie2 HISAT Center for Computational Biology at Johns Hopkins University https://ccb.jhu.edu/software/hisat ImageJ NIH Image https://imagej.net/imagej-wiki-static/ImageJ DESeq2 Bioconductor https://bioconductor.org/packages/release/ bioc/html/DESeq2.html R statistical software The R Project https://www.r-project.org (Continued on next page) e2 iScience 29, 115950, June 19, 2026
Control:Article Title: ALDH1A3 promotes the lung metastatic organotropism of pancreatic ductal adenocarcinoma through semaphorin signaling.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Magna ChIP A/G Chromatin Immunoprecipitation Kit Merck Cat# 17-10085 Nuclear and cytoplasmic protein extraction kit Beyotime Cat# P0027 Deposited data RNA Sequencing data This paper GEOID: GSE248375 RNA Sequencing data This paper HRA011000 Multi-omic profiling of lung and liver tumor microenvironments of metastatic pancreatic cancer reveals site-specific immuneregulatory features NCBI Gene Expression Omnibus GEOID: GSE172104 Experimental models: Cell lines Mouse PDAC cell lines: 399, 403, 445,897,907,908,928, 952 and 5M409L p48cre; KrasG12D/+; Tsc1fl/+ mice N/A Mouse PDAC cell lines 5M409L p48cre; KrasG12D/+; p53 fl/fl mice N/A Mouse LPCs This paper N/A HPAC ATCC Cat# CRL-2119; RRID: CVCL_3517 AsPC-1 ATCC Cat# CRL-1682; RRID: CVCL_0152 MLE-12 ATCC Cat# CRL-2110, RRID:CVCL_3751 Experimental models: Organisms/strains C57BL/6J Charles River Strain #213 BALB/c Nude Charles River Strain #401 Oligonucleotides Primers for real-time PCR analysis, see Supplemental information This paper N/A Primers for CHIP PCR analysis, see Supplemental information This paper N/A Human Aldh1a3 shRNA, see Supplemental information This paper N/A Mouse Plxnb1 shRNA, see Supplemental information This paper N/A Mouse Nrp1 shRNA, see Supplemental information This paper N/A Mouse Sema3a siRNA, see Supplemental information This paper N/A Recombinant DNA Mouse Aldh1a3 Plasmid Origene Cat# MG222097 Mouse Plxnb1 Plasmid Origene Cat# MR222673 Mouse Nrp1 Plasmid Origene Cat# MR211197 pCMV6-Entry Plasmid Origene Cat# PS100001 Mouse Aldh1a3 CRISPR Plasmid Santa Cruz Biotechnology Cat# sc-425322 Software and algorithms GraphPad Prism software GraphPad Software https://www.graphpad.com/ Bowtie2 SourceForge https://bowtie-bio.sourceforge.net/bowtie2 HISAT Center for Computational Biology at Johns Hopkins University https://ccb.jhu.edu/software/hisat ImageJ NIH Image https://imagej.net/imagej-wiki-static/ImageJ DESeq2 Bioconductor https://bioconductor.org/packages/release/ bioc/html/DESeq2.html R statistical software The R Project https://www.r-project.org (Continued on next page) e2 iScience 29, 115950, June 19, 2026
Western Blot:Article Title: ALDH1A3 promotes the lung metastatic organotropism of pancreatic ductal adenocarcinoma through semaphorin signaling.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Magna ChIP A/G Chromatin Immunoprecipitation Kit Merck Cat# 17-10085 Nuclear and cytoplasmic protein extraction kit Beyotime Cat# P0027 Deposited data RNA Sequencing data This paper GEOID: GSE248375 RNA Sequencing data This paper HRA011000 Multi-omic profiling of lung and liver tumor microenvironments of metastatic pancreatic cancer reveals site-specific immuneregulatory features NCBI Gene Expression Omnibus GEOID: GSE172104 Experimental models: Cell lines Mouse PDAC cell lines: 399, 403, 445,897,907,908,928, 952 and 5M409L p48cre; KrasG12D/+; Tsc1fl/+ mice N/A Mouse PDAC cell lines 5M409L p48cre; KrasG12D/+; p53 fl/fl mice N/A Mouse LPCs This paper N/A HPAC ATCC Cat# CRL-2119; RRID: CVCL_3517 AsPC-1 ATCC Cat# CRL-1682; RRID: CVCL_0152 MLE-12 ATCC Cat# CRL-2110, RRID:CVCL_3751 Experimental models: Organisms/strains C57BL/6J Charles River Strain #213 BALB/c Nude Charles River Strain #401 Oligonucleotides Primers for real-time PCR analysis, see Supplemental information This paper N/A Primers for CHIP PCR analysis, see Supplemental information This paper N/A Human Aldh1a3 shRNA, see Supplemental information This paper N/A Mouse Plxnb1 shRNA, see Supplemental information This paper N/A Mouse Nrp1 shRNA, see Supplemental information This paper N/A Mouse Sema3a siRNA, see Supplemental information This paper N/A Recombinant DNA Mouse Aldh1a3 Plasmid Origene Cat# MG222097 Mouse Plxnb1 Plasmid Origene Cat# MR222673 Mouse Nrp1 Plasmid Origene Cat# MR211197 pCMV6-Entry Plasmid Origene Cat# PS100001 Mouse Aldh1a3 CRISPR Plasmid Santa Cruz Biotechnology Cat# sc-425322 Software and algorithms GraphPad Prism software GraphPad Software https://www.graphpad.com/ Bowtie2 SourceForge https://bowtie-bio.sourceforge.net/bowtie2 HISAT Center for Computational Biology at Johns Hopkins University https://ccb.jhu.edu/software/hisat ImageJ NIH Image https://imagej.net/imagej-wiki-static/ImageJ DESeq2 Bioconductor https://bioconductor.org/packages/release/ bioc/html/DESeq2.html R statistical software The R Project https://www.r-project.org (Continued on next page) e2 iScience 29, 115950, June 19, 2026
Immunofluorescence:Article Title: ALDH1A3 promotes the lung metastatic organotropism of pancreatic ductal adenocarcinoma through semaphorin signaling.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Magna ChIP A/G Chromatin Immunoprecipitation Kit Merck Cat# 17-10085 Nuclear and cytoplasmic protein extraction kit Beyotime Cat# P0027 Deposited data RNA Sequencing data This paper GEOID: GSE248375 RNA Sequencing data This paper HRA011000 Multi-omic profiling of lung and liver tumor microenvironments of metastatic pancreatic cancer reveals site-specific immuneregulatory features NCBI Gene Expression Omnibus GEOID: GSE172104 Experimental models: Cell lines Mouse PDAC cell lines: 399, 403, 445,897,907,908,928, 952 and 5M409L p48cre; KrasG12D/+; Tsc1fl/+ mice N/A Mouse PDAC cell lines 5M409L p48cre; KrasG12D/+; p53 fl/fl mice N/A Mouse LPCs This paper N/A HPAC ATCC Cat# CRL-2119; RRID: CVCL_3517 AsPC-1 ATCC Cat# CRL-1682; RRID: CVCL_0152 MLE-12 ATCC Cat# CRL-2110, RRID:CVCL_3751 Experimental models: Organisms/strains C57BL/6J Charles River Strain #213 BALB/c Nude Charles River Strain #401 Oligonucleotides Primers for real-time PCR analysis, see Supplemental information This paper N/A Primers for CHIP PCR analysis, see Supplemental information This paper N/A Human Aldh1a3 shRNA, see Supplemental information This paper N/A Mouse Plxnb1 shRNA, see Supplemental information This paper N/A Mouse Nrp1 shRNA, see Supplemental information This paper N/A Mouse Sema3a siRNA, see Supplemental information This paper N/A Recombinant DNA Mouse Aldh1a3 Plasmid Origene Cat# MG222097 Mouse Plxnb1 Plasmid Origene Cat# MR222673 Mouse Nrp1 Plasmid Origene Cat# MR211197 pCMV6-Entry Plasmid Origene Cat# PS100001 Mouse Aldh1a3 CRISPR Plasmid Santa Cruz Biotechnology Cat# sc-425322 Software and algorithms GraphPad Prism software GraphPad Software https://www.graphpad.com/ Bowtie2 SourceForge https://bowtie-bio.sourceforge.net/bowtie2 HISAT Center for Computational Biology at Johns Hopkins University https://ccb.jhu.edu/software/hisat ImageJ NIH Image https://imagej.net/imagej-wiki-static/ImageJ DESeq2 Bioconductor https://bioconductor.org/packages/release/ bioc/html/DESeq2.html R statistical software The R Project https://www.r-project.org (Continued on next page) e2 iScience 29, 115950, June 19, 2026
Staining:Article Title: ALDH1A3 promotes the lung metastatic organotropism of pancreatic ductal adenocarcinoma through semaphorin signaling.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Magna ChIP A/G Chromatin Immunoprecipitation Kit Merck Cat# 17-10085 Nuclear and cytoplasmic protein extraction kit Beyotime Cat# P0027 Deposited data RNA Sequencing data This paper GEOID: GSE248375 RNA Sequencing data This paper HRA011000 Multi-omic profiling of lung and liver tumor microenvironments of metastatic pancreatic cancer reveals site-specific immuneregulatory features NCBI Gene Expression Omnibus GEOID: GSE172104 Experimental models: Cell lines Mouse PDAC cell lines: 399, 403, 445,897,907,908,928, 952 and 5M409L p48cre; KrasG12D/+; Tsc1fl/+ mice N/A Mouse PDAC cell lines 5M409L p48cre; KrasG12D/+; p53 fl/fl mice N/A Mouse LPCs This paper N/A HPAC ATCC Cat# CRL-2119; RRID: CVCL_3517 AsPC-1 ATCC Cat# CRL-1682; RRID: CVCL_0152 MLE-12 ATCC Cat# CRL-2110, RRID:CVCL_3751 Experimental models: Organisms/strains C57BL/6J Charles River Strain #213 BALB/c Nude Charles River Strain #401 Oligonucleotides Primers for real-time PCR analysis, see Supplemental information This paper N/A Primers for CHIP PCR analysis, see Supplemental information This paper N/A Human Aldh1a3 shRNA, see Supplemental information This paper N/A Mouse Plxnb1 shRNA, see Supplemental information This paper N/A Mouse Nrp1 shRNA, see Supplemental information This paper N/A Mouse Sema3a siRNA, see Supplemental information This paper N/A Recombinant DNA Mouse Aldh1a3 Plasmid Origene Cat# MG222097 Mouse Plxnb1 Plasmid Origene Cat# MR222673 Mouse Nrp1 Plasmid Origene Cat# MR211197 pCMV6-Entry Plasmid Origene Cat# PS100001 Mouse Aldh1a3 CRISPR Plasmid Santa Cruz Biotechnology Cat# sc-425322 Software and algorithms GraphPad Prism software GraphPad Software https://www.graphpad.com/ Bowtie2 SourceForge https://bowtie-bio.sourceforge.net/bowtie2 HISAT Center for Computational Biology at Johns Hopkins University https://ccb.jhu.edu/software/hisat ImageJ NIH Image https://imagej.net/imagej-wiki-static/ImageJ DESeq2 Bioconductor https://bioconductor.org/packages/release/ bioc/html/DESeq2.html R statistical software The R Project https://www.r-project.org (Continued on next page) e2 iScience 29, 115950, June 19, 2026
Live Cell Imaging:Article Title: ALDH1A3 promotes the lung metastatic organotropism of pancreatic ductal adenocarcinoma through semaphorin signaling.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Magna ChIP A/G Chromatin Immunoprecipitation Kit Merck Cat# 17-10085 Nuclear and cytoplasmic protein extraction kit Beyotime Cat# P0027 Deposited data RNA Sequencing data This paper GEOID: GSE248375 RNA Sequencing data This paper HRA011000 Multi-omic profiling of lung and liver tumor microenvironments of metastatic pancreatic cancer reveals site-specific immuneregulatory features NCBI Gene Expression Omnibus GEOID: GSE172104 Experimental models: Cell lines Mouse PDAC cell lines: 399, 403, 445,897,907,908,928, 952 and 5M409L p48cre; KrasG12D/+; Tsc1fl/+ mice N/A Mouse PDAC cell lines 5M409L p48cre; KrasG12D/+; p53 fl/fl mice N/A Mouse LPCs This paper N/A HPAC ATCC Cat# CRL-2119; RRID: CVCL_3517 AsPC-1 ATCC Cat# CRL-1682; RRID: CVCL_0152 MLE-12 ATCC Cat# CRL-2110, RRID:CVCL_3751 Experimental models: Organisms/strains C57BL/6J Charles River Strain #213 BALB/c Nude Charles River Strain #401 Oligonucleotides Primers for real-time PCR analysis, see Supplemental information This paper N/A Primers for CHIP PCR analysis, see Supplemental information This paper N/A Human Aldh1a3 shRNA, see Supplemental information This paper N/A Mouse Plxnb1 shRNA, see Supplemental information This paper N/A Mouse Nrp1 shRNA, see Supplemental information This paper N/A Mouse Sema3a siRNA, see Supplemental information This paper N/A Recombinant DNA Mouse Aldh1a3 Plasmid Origene Cat# MG222097 Mouse Plxnb1 Plasmid Origene Cat# MR222673 Mouse Nrp1 Plasmid Origene Cat# MR211197 pCMV6-Entry Plasmid Origene Cat# PS100001 Mouse Aldh1a3 CRISPR Plasmid Santa Cruz Biotechnology Cat# sc-425322 Software and algorithms GraphPad Prism software GraphPad Software https://www.graphpad.com/ Bowtie2 SourceForge https://bowtie-bio.sourceforge.net/bowtie2 HISAT Center for Computational Biology at Johns Hopkins University https://ccb.jhu.edu/software/hisat ImageJ NIH Image https://imagej.net/imagej-wiki-static/ImageJ DESeq2 Bioconductor https://bioconductor.org/packages/release/ bioc/html/DESeq2.html R statistical software The R Project https://www.r-project.org (Continued on next page) e2 iScience 29, 115950, June 19, 2026
RNA Sequencing:Article Title: ALDH1A3 promotes the lung metastatic organotropism of pancreatic ductal adenocarcinoma through semaphorin signaling.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Magna ChIP A/G Chromatin Immunoprecipitation Kit Merck Cat# 17-10085 Nuclear and cytoplasmic protein extraction kit Beyotime Cat# P0027 Deposited data RNA Sequencing data This paper GEOID: GSE248375 RNA Sequencing data This paper HRA011000 Multi-omic profiling of lung and liver tumor microenvironments of metastatic pancreatic cancer reveals site-specific immuneregulatory features NCBI Gene Expression Omnibus GEOID: GSE172104 Experimental models: Cell lines Mouse PDAC cell lines: 399, 403, 445,897,907,908,928, 952 and 5M409L p48cre; KrasG12D/+; Tsc1fl/+ mice N/A Mouse PDAC cell lines 5M409L p48cre; KrasG12D/+; p53 fl/fl mice N/A Mouse LPCs This paper N/A HPAC ATCC Cat# CRL-2119; RRID: CVCL_3517 AsPC-1 ATCC Cat# CRL-1682; RRID: CVCL_0152 MLE-12 ATCC Cat# CRL-2110, RRID:CVCL_3751 Experimental models: Organisms/strains C57BL/6J Charles River Strain #213 BALB/c Nude Charles River Strain #401 Oligonucleotides Primers for real-time PCR analysis, see Supplemental information This paper N/A Primers for CHIP PCR analysis, see Supplemental information This paper N/A Human Aldh1a3 shRNA, see Supplemental information This paper N/A Mouse Plxnb1 shRNA, see Supplemental information This paper N/A Mouse Nrp1 shRNA, see Supplemental information This paper N/A Mouse Sema3a siRNA, see Supplemental information This paper N/A Recombinant DNA Mouse Aldh1a3 Plasmid Origene Cat# MG222097 Mouse Plxnb1 Plasmid Origene Cat# MR222673 Mouse Nrp1 Plasmid Origene Cat# MR211197 pCMV6-Entry Plasmid Origene Cat# PS100001 Mouse Aldh1a3 CRISPR Plasmid Santa Cruz Biotechnology Cat# sc-425322 Software and algorithms GraphPad Prism software GraphPad Software https://www.graphpad.com/ Bowtie2 SourceForge https://bowtie-bio.sourceforge.net/bowtie2 HISAT Center for Computational Biology at Johns Hopkins University https://ccb.jhu.edu/software/hisat ImageJ NIH Image https://imagej.net/imagej-wiki-static/ImageJ DESeq2 Bioconductor https://bioconductor.org/packages/release/ bioc/html/DESeq2.html R statistical software The R Project https://www.r-project.org (Continued on next page) e2 iScience 29, 115950, June 19, 2026
Binding Assay:Article Title: ALDH1A3 promotes the lung metastatic organotropism of pancreatic ductal adenocarcinoma through semaphorin signaling.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Magna ChIP A/G Chromatin Immunoprecipitation Kit Merck Cat# 17-10085 Nuclear and cytoplasmic protein extraction kit Beyotime Cat# P0027 Deposited data RNA Sequencing data This paper GEOID: GSE248375 RNA Sequencing data This paper HRA011000 Multi-omic profiling of lung and liver tumor microenvironments of metastatic pancreatic cancer reveals site-specific immuneregulatory features NCBI Gene Expression Omnibus GEOID: GSE172104 Experimental models: Cell lines Mouse PDAC cell lines: 399, 403, 445,897,907,908,928, 952 and 5M409L p48cre; KrasG12D/+; Tsc1fl/+ mice N/A Mouse PDAC cell lines 5M409L p48cre; KrasG12D/+; p53 fl/fl mice N/A Mouse LPCs This paper N/A HPAC ATCC Cat# CRL-2119; RRID: CVCL_3517 AsPC-1 ATCC Cat# CRL-1682; RRID: CVCL_0152 MLE-12 ATCC Cat# CRL-2110, RRID:CVCL_3751 Experimental models: Organisms/strains C57BL/6J Charles River Strain #213 BALB/c Nude Charles River Strain #401 Oligonucleotides Primers for real-time PCR analysis, see Supplemental information This paper N/A Primers for CHIP PCR analysis, see Supplemental information This paper N/A Human Aldh1a3 shRNA, see Supplemental information This paper N/A Mouse Plxnb1 shRNA, see Supplemental information This paper N/A Mouse Nrp1 shRNA, see Supplemental information This paper N/A Mouse Sema3a siRNA, see Supplemental information This paper N/A Recombinant DNA Mouse Aldh1a3 Plasmid Origene Cat# MG222097 Mouse Plxnb1 Plasmid Origene Cat# MR222673 Mouse Nrp1 Plasmid Origene Cat# MR211197 pCMV6-Entry Plasmid Origene Cat# PS100001 Mouse Aldh1a3 CRISPR Plasmid Santa Cruz Biotechnology Cat# sc-425322 Software and algorithms GraphPad Prism software GraphPad Software https://www.graphpad.com/ Bowtie2 SourceForge https://bowtie-bio.sourceforge.net/bowtie2 HISAT Center for Computational Biology at Johns Hopkins University https://ccb.jhu.edu/software/hisat ImageJ NIH Image https://imagej.net/imagej-wiki-static/ImageJ DESeq2 Bioconductor https://bioconductor.org/packages/release/ bioc/html/DESeq2.html R statistical software The R Project https://www.r-project.org (Continued on next page) e2 iScience 29, 115950, June 19, 2026
IV Injection:Article Title: ALDH1A3 promotes the lung metastatic organotropism of pancreatic ductal adenocarcinoma through semaphorin signaling.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Magna ChIP A/G Chromatin Immunoprecipitation Kit Merck Cat# 17-10085 Nuclear and cytoplasmic protein extraction kit Beyotime Cat# P0027 Deposited data RNA Sequencing data This paper GEOID: GSE248375 RNA Sequencing data This paper HRA011000 Multi-omic profiling of lung and liver tumor microenvironments of metastatic pancreatic cancer reveals site-specific immuneregulatory features NCBI Gene Expression Omnibus GEOID: GSE172104 Experimental models: Cell lines Mouse PDAC cell lines: 399, 403, 445,897,907,908,928, 952 and 5M409L p48cre; KrasG12D/+; Tsc1fl/+ mice N/A Mouse PDAC cell lines 5M409L p48cre; KrasG12D/+; p53 fl/fl mice N/A Mouse LPCs This paper N/A HPAC ATCC Cat# CRL-2119; RRID: CVCL_3517 AsPC-1 ATCC Cat# CRL-1682; RRID: CVCL_0152 MLE-12 ATCC Cat# CRL-2110, RRID:CVCL_3751 Experimental models: Organisms/strains C57BL/6J Charles River Strain #213 BALB/c Nude Charles River Strain #401 Oligonucleotides Primers for real-time PCR analysis, see Supplemental information This paper N/A Primers for CHIP PCR analysis, see Supplemental information This paper N/A Human Aldh1a3 shRNA, see Supplemental information This paper N/A Mouse Plxnb1 shRNA, see Supplemental information This paper N/A Mouse Nrp1 shRNA, see Supplemental information This paper N/A Mouse Sema3a siRNA, see Supplemental information This paper N/A Recombinant DNA Mouse Aldh1a3 Plasmid Origene Cat# MG222097 Mouse Plxnb1 Plasmid Origene Cat# MR222673 Mouse Nrp1 Plasmid Origene Cat# MR211197 pCMV6-Entry Plasmid Origene Cat# PS100001 Mouse Aldh1a3 CRISPR Plasmid Santa Cruz Biotechnology Cat# sc-425322 Software and algorithms GraphPad Prism software GraphPad Software https://www.graphpad.com/ Bowtie2 SourceForge https://bowtie-bio.sourceforge.net/bowtie2 HISAT Center for Computational Biology at Johns Hopkins University https://ccb.jhu.edu/software/hisat ImageJ NIH Image https://imagej.net/imagej-wiki-static/ImageJ DESeq2 Bioconductor https://bioconductor.org/packages/release/ bioc/html/DESeq2.html R statistical software The R Project https://www.r-project.org (Continued on next page) e2 iScience 29, 115950, June 19, 2026
Enzyme-linked Immunosorbent Assay:Article Title: ALDH1A3 promotes the lung metastatic organotropism of pancreatic ductal adenocarcinoma through semaphorin signaling.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Magna ChIP A/G Chromatin Immunoprecipitation Kit Merck Cat# 17-10085 Nuclear and cytoplasmic protein extraction kit Beyotime Cat# P0027 Deposited data RNA Sequencing data This paper GEOID: GSE248375 RNA Sequencing data This paper HRA011000 Multi-omic profiling of lung and liver tumor microenvironments of metastatic pancreatic cancer reveals site-specific immuneregulatory features NCBI Gene Expression Omnibus GEOID: GSE172104 Experimental models: Cell lines Mouse PDAC cell lines: 399, 403, 445,897,907,908,928, 952 and 5M409L p48cre; KrasG12D/+; Tsc1fl/+ mice N/A Mouse PDAC cell lines 5M409L p48cre; KrasG12D/+; p53 fl/fl mice N/A Mouse LPCs This paper N/A HPAC ATCC Cat# CRL-2119; RRID: CVCL_3517 AsPC-1 ATCC Cat# CRL-1682; RRID: CVCL_0152 MLE-12 ATCC Cat# CRL-2110, RRID:CVCL_3751 Experimental models: Organisms/strains C57BL/6J Charles River Strain #213 BALB/c Nude Charles River Strain #401 Oligonucleotides Primers for real-time PCR analysis, see Supplemental information This paper N/A Primers for CHIP PCR analysis, see Supplemental information This paper N/A Human Aldh1a3 shRNA, see Supplemental information This paper N/A Mouse Plxnb1 shRNA, see Supplemental information This paper N/A Mouse Nrp1 shRNA, see Supplemental information This paper N/A Mouse Sema3a siRNA, see Supplemental information This paper N/A Recombinant DNA Mouse Aldh1a3 Plasmid Origene Cat# MG222097 Mouse Plxnb1 Plasmid Origene Cat# MR222673 Mouse Nrp1 Plasmid Origene Cat# MR211197 pCMV6-Entry Plasmid Origene Cat# PS100001 Mouse Aldh1a3 CRISPR Plasmid Santa Cruz Biotechnology Cat# sc-425322 Software and algorithms GraphPad Prism software GraphPad Software https://www.graphpad.com/ Bowtie2 SourceForge https://bowtie-bio.sourceforge.net/bowtie2 HISAT Center for Computational Biology at Johns Hopkins University https://ccb.jhu.edu/software/hisat ImageJ NIH Image https://imagej.net/imagej-wiki-static/ImageJ DESeq2 Bioconductor https://bioconductor.org/packages/release/ bioc/html/DESeq2.html R statistical software The R Project https://www.r-project.org (Continued on next page) e2 iScience 29, 115950, June 19, 2026
Xenograft Assay:Article Title: ALDH1A3 promotes the lung metastatic organotropism of pancreatic ductal adenocarcinoma through semaphorin signaling.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Magna ChIP A/G Chromatin Immunoprecipitation Kit Merck Cat# 17-10085 Nuclear and cytoplasmic protein extraction kit Beyotime Cat# P0027 Deposited data RNA Sequencing data This paper GEOID: GSE248375 RNA Sequencing data This paper HRA011000 Multi-omic profiling of lung and liver tumor microenvironments of metastatic pancreatic cancer reveals site-specific immuneregulatory features NCBI Gene Expression Omnibus GEOID: GSE172104 Experimental models: Cell lines Mouse PDAC cell lines: 399, 403, 445,897,907,908,928, 952 and 5M409L p48cre; KrasG12D/+; Tsc1fl/+ mice N/A Mouse PDAC cell lines 5M409L p48cre; KrasG12D/+; p53 fl/fl mice N/A Mouse LPCs This paper N/A HPAC ATCC Cat# CRL-2119; RRID: CVCL_3517 AsPC-1 ATCC Cat# CRL-1682; RRID: CVCL_0152 MLE-12 ATCC Cat# CRL-2110, RRID:CVCL_3751 Experimental models: Organisms/strains C57BL/6J Charles River Strain #213 BALB/c Nude Charles River Strain #401 Oligonucleotides Primers for real-time PCR analysis, see Supplemental information This paper N/A Primers for CHIP PCR analysis, see Supplemental information This paper N/A Human Aldh1a3 shRNA, see Supplemental information This paper N/A Mouse Plxnb1 shRNA, see Supplemental information This paper N/A Mouse Nrp1 shRNA, see Supplemental information This paper N/A Mouse Sema3a siRNA, see Supplemental information This paper N/A Recombinant DNA Mouse Aldh1a3 Plasmid Origene Cat# MG222097 Mouse Plxnb1 Plasmid Origene Cat# MR222673 Mouse Nrp1 Plasmid Origene Cat# MR211197 pCMV6-Entry Plasmid Origene Cat# PS100001 Mouse Aldh1a3 CRISPR Plasmid Santa Cruz Biotechnology Cat# sc-425322 Software and algorithms GraphPad Prism software GraphPad Software https://www.graphpad.com/ Bowtie2 SourceForge https://bowtie-bio.sourceforge.net/bowtie2 HISAT Center for Computational Biology at Johns Hopkins University https://ccb.jhu.edu/software/hisat ImageJ NIH Image https://imagej.net/imagej-wiki-static/ImageJ DESeq2 Bioconductor https://bioconductor.org/packages/release/ bioc/html/DESeq2.html R statistical software The R Project https://www.r-project.org (Continued on next page) e2 iScience 29, 115950, June 19, 2026
FACS:Article Title: ALDH1A3 promotes the lung metastatic organotropism of pancreatic ductal adenocarcinoma through semaphorin signaling.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Magna ChIP A/G Chromatin Immunoprecipitation Kit Merck Cat# 17-10085 Nuclear and cytoplasmic protein extraction kit Beyotime Cat# P0027 Deposited data RNA Sequencing data This paper GEOID: GSE248375 RNA Sequencing data This paper HRA011000 Multi-omic profiling of lung and liver tumor microenvironments of metastatic pancreatic cancer reveals site-specific immuneregulatory features NCBI Gene Expression Omnibus GEOID: GSE172104 Experimental models: Cell lines Mouse PDAC cell lines: 399, 403, 445,897,907,908,928, 952 and 5M409L p48cre; KrasG12D/+; Tsc1fl/+ mice N/A Mouse PDAC cell lines 5M409L p48cre; KrasG12D/+; p53 fl/fl mice N/A Mouse LPCs This paper N/A HPAC ATCC Cat# CRL-2119; RRID: CVCL_3517 AsPC-1 ATCC Cat# CRL-1682; RRID: CVCL_0152 MLE-12 ATCC Cat# CRL-2110, RRID:CVCL_3751 Experimental models: Organisms/strains C57BL/6J Charles River Strain #213 BALB/c Nude Charles River Strain #401 Oligonucleotides Primers for real-time PCR analysis, see Supplemental information This paper N/A Primers for CHIP PCR analysis, see Supplemental information This paper N/A Human Aldh1a3 shRNA, see Supplemental information This paper N/A Mouse Plxnb1 shRNA, see Supplemental information This paper N/A Mouse Nrp1 shRNA, see Supplemental information This paper N/A Mouse Sema3a siRNA, see Supplemental information This paper N/A Recombinant DNA Mouse Aldh1a3 Plasmid Origene Cat# MG222097 Mouse Plxnb1 Plasmid Origene Cat# MR222673 Mouse Nrp1 Plasmid Origene Cat# MR211197 pCMV6-Entry Plasmid Origene Cat# PS100001 Mouse Aldh1a3 CRISPR Plasmid Santa Cruz Biotechnology Cat# sc-425322 Software and algorithms GraphPad Prism software GraphPad Software https://www.graphpad.com/ Bowtie2 SourceForge https://bowtie-bio.sourceforge.net/bowtie2 HISAT Center for Computational Biology at Johns Hopkins University https://ccb.jhu.edu/software/hisat ImageJ NIH Image https://imagej.net/imagej-wiki-static/ImageJ DESeq2 Bioconductor https://bioconductor.org/packages/release/ bioc/html/DESeq2.html R statistical software The R Project https://www.r-project.org (Continued on next page) e2 iScience 29, 115950, June 19, 2026
Immunohistochemistry:Article Title: ALDH1A3 promotes the lung metastatic organotropism of pancreatic ductal adenocarcinoma through semaphorin signaling.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Magna ChIP A/G Chromatin Immunoprecipitation Kit Merck Cat# 17-10085 Nuclear and cytoplasmic protein extraction kit Beyotime Cat# P0027 Deposited data RNA Sequencing data This paper GEOID: GSE248375 RNA Sequencing data This paper HRA011000 Multi-omic profiling of lung and liver tumor microenvironments of metastatic pancreatic cancer reveals site-specific immuneregulatory features NCBI Gene Expression Omnibus GEOID: GSE172104 Experimental models: Cell lines Mouse PDAC cell lines: 399, 403, 445,897,907,908,928, 952 and 5M409L p48cre; KrasG12D/+; Tsc1fl/+ mice N/A Mouse PDAC cell lines 5M409L p48cre; KrasG12D/+; p53 fl/fl mice N/A Mouse LPCs This paper N/A HPAC ATCC Cat# CRL-2119; RRID: CVCL_3517 AsPC-1 ATCC Cat# CRL-1682; RRID: CVCL_0152 MLE-12 ATCC Cat# CRL-2110, RRID:CVCL_3751 Experimental models: Organisms/strains C57BL/6J Charles River Strain #213 BALB/c Nude Charles River Strain #401 Oligonucleotides Primers for real-time PCR analysis, see Supplemental information This paper N/A Primers for CHIP PCR analysis, see Supplemental information This paper N/A Human Aldh1a3 shRNA, see Supplemental information This paper N/A Mouse Plxnb1 shRNA, see Supplemental information This paper N/A Mouse Nrp1 shRNA, see Supplemental information This paper N/A Mouse Sema3a siRNA, see Supplemental information This paper N/A Recombinant DNA Mouse Aldh1a3 Plasmid Origene Cat# MG222097 Mouse Plxnb1 Plasmid Origene Cat# MR222673 Mouse Nrp1 Plasmid Origene Cat# MR211197 pCMV6-Entry Plasmid Origene Cat# PS100001 Mouse Aldh1a3 CRISPR Plasmid Santa Cruz Biotechnology Cat# sc-425322 Software and algorithms GraphPad Prism software GraphPad Software https://www.graphpad.com/ Bowtie2 SourceForge https://bowtie-bio.sourceforge.net/bowtie2 HISAT Center for Computational Biology at Johns Hopkins University https://ccb.jhu.edu/software/hisat ImageJ NIH Image https://imagej.net/imagej-wiki-static/ImageJ DESeq2 Bioconductor https://bioconductor.org/packages/release/ bioc/html/DESeq2.html R statistical software The R Project https://www.r-project.org (Continued on next page) e2 iScience 29, 115950, June 19, 2026
Chromatin Immunoprecipitation:Article Title: ALDH1A3 promotes the lung metastatic organotropism of pancreatic ductal adenocarcinoma through semaphorin signaling.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Magna ChIP A/G Chromatin Immunoprecipitation Kit Merck Cat# 17-10085 Nuclear and cytoplasmic protein extraction kit Beyotime Cat# P0027 Deposited data RNA Sequencing data This paper GEOID: GSE248375 RNA Sequencing data This paper HRA011000 Multi-omic profiling of lung and liver tumor microenvironments of metastatic pancreatic cancer reveals site-specific immuneregulatory features NCBI Gene Expression Omnibus GEOID: GSE172104 Experimental models: Cell lines Mouse PDAC cell lines: 399, 403, 445,897,907,908,928, 952 and 5M409L p48cre; KrasG12D/+; Tsc1fl/+ mice N/A Mouse PDAC cell lines 5M409L p48cre; KrasG12D/+; p53 fl/fl mice N/A Mouse LPCs This paper N/A HPAC ATCC Cat# CRL-2119; RRID: CVCL_3517 AsPC-1 ATCC Cat# CRL-1682; RRID: CVCL_0152 MLE-12 ATCC Cat# CRL-2110, RRID:CVCL_3751 Experimental models: Organisms/strains C57BL/6J Charles River Strain #213 BALB/c Nude Charles River Strain #401 Oligonucleotides Primers for real-time PCR analysis, see Supplemental information This paper N/A Primers for CHIP PCR analysis, see Supplemental information This paper N/A Human Aldh1a3 shRNA, see Supplemental information This paper N/A Mouse Plxnb1 shRNA, see Supplemental information This paper N/A Mouse Nrp1 shRNA, see Supplemental information This paper N/A Mouse Sema3a siRNA, see Supplemental information This paper N/A Recombinant DNA Mouse Aldh1a3 Plasmid Origene Cat# MG222097 Mouse Plxnb1 Plasmid Origene Cat# MR222673 Mouse Nrp1 Plasmid Origene Cat# MR211197 pCMV6-Entry Plasmid Origene Cat# PS100001 Mouse Aldh1a3 CRISPR Plasmid Santa Cruz Biotechnology Cat# sc-425322 Software and algorithms GraphPad Prism software GraphPad Software https://www.graphpad.com/ Bowtie2 SourceForge https://bowtie-bio.sourceforge.net/bowtie2 HISAT Center for Computational Biology at Johns Hopkins University https://ccb.jhu.edu/software/hisat ImageJ NIH Image https://imagej.net/imagej-wiki-static/ImageJ DESeq2 Bioconductor https://bioconductor.org/packages/release/ bioc/html/DESeq2.html R statistical software The R Project https://www.r-project.org (Continued on next page) e2 iScience 29, 115950, June 19, 2026
Protein Extraction:Article Title: ALDH1A3 promotes the lung metastatic organotropism of pancreatic ductal adenocarcinoma through semaphorin signaling.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Magna ChIP A/G Chromatin Immunoprecipitation Kit Merck Cat# 17-10085 Nuclear and cytoplasmic protein extraction kit Beyotime Cat# P0027 Deposited data RNA Sequencing data This paper GEOID: GSE248375 RNA Sequencing data This paper HRA011000 Multi-omic profiling of lung and liver tumor microenvironments of metastatic pancreatic cancer reveals site-specific immuneregulatory features NCBI Gene Expression Omnibus GEOID: GSE172104 Experimental models: Cell lines Mouse PDAC cell lines: 399, 403, 445,897,907,908,928, 952 and 5M409L p48cre; KrasG12D/+; Tsc1fl/+ mice N/A Mouse PDAC cell lines 5M409L p48cre; KrasG12D/+; p53 fl/fl mice N/A Mouse LPCs This paper N/A HPAC ATCC Cat# CRL-2119; RRID: CVCL_3517 AsPC-1 ATCC Cat# CRL-1682; RRID: CVCL_0152 MLE-12 ATCC Cat# CRL-2110, RRID:CVCL_3751 Experimental models: Organisms/strains C57BL/6J Charles River Strain #213 BALB/c Nude Charles River Strain #401 Oligonucleotides Primers for real-time PCR analysis, see Supplemental information This paper N/A Primers for CHIP PCR analysis, see Supplemental information This paper N/A Human Aldh1a3 shRNA, see Supplemental information This paper N/A Mouse Plxnb1 shRNA, see Supplemental information This paper N/A Mouse Nrp1 shRNA, see Supplemental information This paper N/A Mouse Sema3a siRNA, see Supplemental information This paper N/A Recombinant DNA Mouse Aldh1a3 Plasmid Origene Cat# MG222097 Mouse Plxnb1 Plasmid Origene Cat# MR222673 Mouse Nrp1 Plasmid Origene Cat# MR211197 pCMV6-Entry Plasmid Origene Cat# PS100001 Mouse Aldh1a3 CRISPR Plasmid Santa Cruz Biotechnology Cat# sc-425322 Software and algorithms GraphPad Prism software GraphPad Software https://www.graphpad.com/ Bowtie2 SourceForge https://bowtie-bio.sourceforge.net/bowtie2 HISAT Center for Computational Biology at Johns Hopkins University https://ccb.jhu.edu/software/hisat ImageJ NIH Image https://imagej.net/imagej-wiki-static/ImageJ DESeq2 Bioconductor https://bioconductor.org/packages/release/ bioc/html/DESeq2.html R statistical software The R Project https://www.r-project.org (Continued on next page) e2 iScience 29, 115950, June 19, 2026
Gene Expression:Article Title: ALDH1A3 promotes the lung metastatic organotropism of pancreatic ductal adenocarcinoma through semaphorin signaling.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Magna ChIP A/G Chromatin Immunoprecipitation Kit Merck Cat# 17-10085 Nuclear and cytoplasmic protein extraction kit Beyotime Cat# P0027 Deposited data RNA Sequencing data This paper GEOID: GSE248375 RNA Sequencing data This paper HRA011000 Multi-omic profiling of lung and liver tumor microenvironments of metastatic pancreatic cancer reveals site-specific immuneregulatory features NCBI Gene Expression Omnibus GEOID: GSE172104 Experimental models: Cell lines Mouse PDAC cell lines: 399, 403, 445,897,907,908,928, 952 and 5M409L p48cre; KrasG12D/+; Tsc1fl/+ mice N/A Mouse PDAC cell lines 5M409L p48cre; KrasG12D/+; p53 fl/fl mice N/A Mouse LPCs This paper N/A HPAC ATCC Cat# CRL-2119; RRID: CVCL_3517 AsPC-1 ATCC Cat# CRL-1682; RRID: CVCL_0152 MLE-12 ATCC Cat# CRL-2110, RRID:CVCL_3751 Experimental models: Organisms/strains C57BL/6J Charles River Strain #213 BALB/c Nude Charles River Strain #401 Oligonucleotides Primers for real-time PCR analysis, see Supplemental information This paper N/A Primers for CHIP PCR analysis, see Supplemental information This paper N/A Human Aldh1a3 shRNA, see Supplemental information This paper N/A Mouse Plxnb1 shRNA, see Supplemental information This paper N/A Mouse Nrp1 shRNA, see Supplemental information This paper N/A Mouse Sema3a siRNA, see Supplemental information This paper N/A Recombinant DNA Mouse Aldh1a3 Plasmid Origene Cat# MG222097 Mouse Plxnb1 Plasmid Origene Cat# MR222673 Mouse Nrp1 Plasmid Origene Cat# MR211197 pCMV6-Entry Plasmid Origene Cat# PS100001 Mouse Aldh1a3 CRISPR Plasmid Santa Cruz Biotechnology Cat# sc-425322 Software and algorithms GraphPad Prism software GraphPad Software https://www.graphpad.com/ Bowtie2 SourceForge https://bowtie-bio.sourceforge.net/bowtie2 HISAT Center for Computational Biology at Johns Hopkins University https://ccb.jhu.edu/software/hisat ImageJ NIH Image https://imagej.net/imagej-wiki-static/ImageJ DESeq2 Bioconductor https://bioconductor.org/packages/release/ bioc/html/DESeq2.html R statistical software The R Project https://www.r-project.org (Continued on next page) e2 iScience 29, 115950, June 19, 2026
Real-time Polymerase Chain Reaction:Article Title: ALDH1A3 promotes the lung metastatic organotropism of pancreatic ductal adenocarcinoma through semaphorin signaling.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Magna ChIP A/G Chromatin Immunoprecipitation Kit Merck Cat# 17-10085 Nuclear and cytoplasmic protein extraction kit Beyotime Cat# P0027 Deposited data RNA Sequencing data This paper GEOID: GSE248375 RNA Sequencing data This paper HRA011000 Multi-omic profiling of lung and liver tumor microenvironments of metastatic pancreatic cancer reveals site-specific immuneregulatory features NCBI Gene Expression Omnibus GEOID: GSE172104 Experimental models: Cell lines Mouse PDAC cell lines: 399, 403, 445,897,907,908,928, 952 and 5M409L p48cre; KrasG12D/+; Tsc1fl/+ mice N/A Mouse PDAC cell lines 5M409L p48cre; KrasG12D/+; p53 fl/fl mice N/A Mouse LPCs This paper N/A HPAC ATCC Cat# CRL-2119; RRID: CVCL_3517 AsPC-1 ATCC Cat# CRL-1682; RRID: CVCL_0152 MLE-12 ATCC Cat# CRL-2110, RRID:CVCL_3751 Experimental models: Organisms/strains C57BL/6J Charles River Strain #213 BALB/c Nude Charles River Strain #401 Oligonucleotides Primers for real-time PCR analysis, see Supplemental information This paper N/A Primers for CHIP PCR analysis, see Supplemental information This paper N/A Human Aldh1a3 shRNA, see Supplemental information This paper N/A Mouse Plxnb1 shRNA, see Supplemental information This paper N/A Mouse Nrp1 shRNA, see Supplemental information This paper N/A Mouse Sema3a siRNA, see Supplemental information This paper N/A Recombinant DNA Mouse Aldh1a3 Plasmid Origene Cat# MG222097 Mouse Plxnb1 Plasmid Origene Cat# MR222673 Mouse Nrp1 Plasmid Origene Cat# MR211197 pCMV6-Entry Plasmid Origene Cat# PS100001 Mouse Aldh1a3 CRISPR Plasmid Santa Cruz Biotechnology Cat# sc-425322 Software and algorithms GraphPad Prism software GraphPad Software https://www.graphpad.com/ Bowtie2 SourceForge https://bowtie-bio.sourceforge.net/bowtie2 HISAT Center for Computational Biology at Johns Hopkins University https://ccb.jhu.edu/software/hisat ImageJ NIH Image https://imagej.net/imagej-wiki-static/ImageJ DESeq2 Bioconductor https://bioconductor.org/packages/release/ bioc/html/DESeq2.html R statistical software The R Project https://www.r-project.org (Continued on next page) e2 iScience 29, 115950, June 19, 2026
Polymerase Chain Reaction:Article Title: ALDH1A3 promotes the lung metastatic organotropism of pancreatic ductal adenocarcinoma through semaphorin signaling.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Magna ChIP A/G Chromatin Immunoprecipitation Kit Merck Cat# 17-10085 Nuclear and cytoplasmic protein extraction kit Beyotime Cat# P0027 Deposited data RNA Sequencing data This paper GEOID: GSE248375 RNA Sequencing data This paper HRA011000 Multi-omic profiling of lung and liver tumor microenvironments of metastatic pancreatic cancer reveals site-specific immuneregulatory features NCBI Gene Expression Omnibus GEOID: GSE172104 Experimental models: Cell lines Mouse PDAC cell lines: 399, 403, 445,897,907,908,928, 952 and 5M409L p48cre; KrasG12D/+; Tsc1fl/+ mice N/A Mouse PDAC cell lines 5M409L p48cre; KrasG12D/+; p53 fl/fl mice N/A Mouse LPCs This paper N/A HPAC ATCC Cat# CRL-2119; RRID: CVCL_3517 AsPC-1 ATCC Cat# CRL-1682; RRID: CVCL_0152 MLE-12 ATCC Cat# CRL-2110, RRID:CVCL_3751 Experimental models: Organisms/strains C57BL/6J Charles River Strain #213 BALB/c Nude Charles River Strain #401 Oligonucleotides Primers for real-time PCR analysis, see Supplemental information This paper N/A Primers for CHIP PCR analysis, see Supplemental information This paper N/A Human Aldh1a3 shRNA, see Supplemental information This paper N/A Mouse Plxnb1 shRNA, see Supplemental information This paper N/A Mouse Nrp1 shRNA, see Supplemental information This paper N/A Mouse Sema3a siRNA, see Supplemental information This paper N/A Recombinant DNA Mouse Aldh1a3 Plasmid Origene Cat# MG222097 Mouse Plxnb1 Plasmid Origene Cat# MR222673 Mouse Nrp1 Plasmid Origene Cat# MR211197 pCMV6-Entry Plasmid Origene Cat# PS100001 Mouse Aldh1a3 CRISPR Plasmid Santa Cruz Biotechnology Cat# sc-425322 Software and algorithms GraphPad Prism software GraphPad Software https://www.graphpad.com/ Bowtie2 SourceForge https://bowtie-bio.sourceforge.net/bowtie2 HISAT Center for Computational Biology at Johns Hopkins University https://ccb.jhu.edu/software/hisat ImageJ NIH Image https://imagej.net/imagej-wiki-static/ImageJ DESeq2 Bioconductor https://bioconductor.org/packages/release/ bioc/html/DESeq2.html R statistical software The R Project https://www.r-project.org (Continued on next page) e2 iScience 29, 115950, June 19, 2026
shRNA:Article Title: ALDH1A3 promotes the lung metastatic organotropism of pancreatic ductal adenocarcinoma through semaphorin signaling.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Magna ChIP A/G Chromatin Immunoprecipitation Kit Merck Cat# 17-10085 Nuclear and cytoplasmic protein extraction kit Beyotime Cat# P0027 Deposited data RNA Sequencing data This paper GEOID: GSE248375 RNA Sequencing data This paper HRA011000 Multi-omic profiling of lung and liver tumor microenvironments of metastatic pancreatic cancer reveals site-specific immuneregulatory features NCBI Gene Expression Omnibus GEOID: GSE172104 Experimental models: Cell lines Mouse PDAC cell lines: 399, 403, 445,897,907,908,928, 952 and 5M409L p48cre; KrasG12D/+; Tsc1fl/+ mice N/A Mouse PDAC cell lines 5M409L p48cre; KrasG12D/+; p53 fl/fl mice N/A Mouse LPCs This paper N/A HPAC ATCC Cat# CRL-2119; RRID: CVCL_3517 AsPC-1 ATCC Cat# CRL-1682; RRID: CVCL_0152 MLE-12 ATCC Cat# CRL-2110, RRID:CVCL_3751 Experimental models: Organisms/strains C57BL/6J Charles River Strain #213 BALB/c Nude Charles River Strain #401 Oligonucleotides Primers for real-time PCR analysis, see Supplemental information This paper N/A Primers for CHIP PCR analysis, see Supplemental information This paper N/A Human Aldh1a3 shRNA, see Supplemental information This paper N/A Mouse Plxnb1 shRNA, see Supplemental information This paper N/A Mouse Nrp1 shRNA, see Supplemental information This paper N/A Mouse Sema3a siRNA, see Supplemental information This paper N/A Recombinant DNA Mouse Aldh1a3 Plasmid Origene Cat# MG222097 Mouse Plxnb1 Plasmid Origene Cat# MR222673 Mouse Nrp1 Plasmid Origene Cat# MR211197 pCMV6-Entry Plasmid Origene Cat# PS100001 Mouse Aldh1a3 CRISPR Plasmid Santa Cruz Biotechnology Cat# sc-425322 Software and algorithms GraphPad Prism software GraphPad Software https://www.graphpad.com/ Bowtie2 SourceForge https://bowtie-bio.sourceforge.net/bowtie2 HISAT Center for Computational Biology at Johns Hopkins University https://ccb.jhu.edu/software/hisat ImageJ NIH Image https://imagej.net/imagej-wiki-static/ImageJ DESeq2 Bioconductor https://bioconductor.org/packages/release/ bioc/html/DESeq2.html R statistical software The R Project https://www.r-project.org (Continued on next page) e2 iScience 29, 115950, June 19, 2026
Recombinant:Article Title: ALDH1A3 promotes the lung metastatic organotropism of pancreatic ductal adenocarcinoma through semaphorin signaling.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Magna ChIP A/G Chromatin Immunoprecipitation Kit Merck Cat# 17-10085 Nuclear and cytoplasmic protein extraction kit Beyotime Cat# P0027 Deposited data RNA Sequencing data This paper GEOID: GSE248375 RNA Sequencing data This paper HRA011000 Multi-omic profiling of lung and liver tumor microenvironments of metastatic pancreatic cancer reveals site-specific immuneregulatory features NCBI Gene Expression Omnibus GEOID: GSE172104 Experimental models: Cell lines Mouse PDAC cell lines: 399, 403, 445,897,907,908,928, 952 and 5M409L p48cre; KrasG12D/+; Tsc1fl/+ mice N/A Mouse PDAC cell lines 5M409L p48cre; KrasG12D/+; p53 fl/fl mice N/A Mouse LPCs This paper N/A HPAC ATCC Cat# CRL-2119; RRID: CVCL_3517 AsPC-1 ATCC Cat# CRL-1682; RRID: CVCL_0152 MLE-12 ATCC Cat# CRL-2110, RRID:CVCL_3751 Experimental models: Organisms/strains C57BL/6J Charles River Strain #213 BALB/c Nude Charles River Strain #401 Oligonucleotides Primers for real-time PCR analysis, see Supplemental information This paper N/A Primers for CHIP PCR analysis, see Supplemental information This paper N/A Human Aldh1a3 shRNA, see Supplemental information This paper N/A Mouse Plxnb1 shRNA, see Supplemental information This paper N/A Mouse Nrp1 shRNA, see Supplemental information This paper N/A Mouse Sema3a siRNA, see Supplemental information This paper N/A Recombinant DNA Mouse Aldh1a3 Plasmid Origene Cat# MG222097 Mouse Plxnb1 Plasmid Origene Cat# MR222673 Mouse Nrp1 Plasmid Origene Cat# MR211197 pCMV6-Entry Plasmid Origene Cat# PS100001 Mouse Aldh1a3 CRISPR Plasmid Santa Cruz Biotechnology Cat# sc-425322 Software and algorithms GraphPad Prism software GraphPad Software https://www.graphpad.com/ Bowtie2 SourceForge https://bowtie-bio.sourceforge.net/bowtie2 HISAT Center for Computational Biology at Johns Hopkins University https://ccb.jhu.edu/software/hisat ImageJ NIH Image https://imagej.net/imagej-wiki-static/ImageJ DESeq2 Bioconductor https://bioconductor.org/packages/release/ bioc/html/DESeq2.html R statistical software The R Project https://www.r-project.org (Continued on next page) e2 iScience 29, 115950, June 19, 2026
Plasmid Preparation:Article Title: ALDH1A3 promotes the lung metastatic organotropism of pancreatic ductal adenocarcinoma through semaphorin signaling.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Magna ChIP A/G Chromatin Immunoprecipitation Kit Merck Cat# 17-10085 Nuclear and cytoplasmic protein extraction kit Beyotime Cat# P0027 Deposited data RNA Sequencing data This paper GEOID: GSE248375 RNA Sequencing data This paper HRA011000 Multi-omic profiling of lung and liver tumor microenvironments of metastatic pancreatic cancer reveals site-specific immuneregulatory features NCBI Gene Expression Omnibus GEOID: GSE172104 Experimental models: Cell lines Mouse PDAC cell lines: 399, 403, 445,897,907,908,928, 952 and 5M409L p48cre; KrasG12D/+; Tsc1fl/+ mice N/A Mouse PDAC cell lines 5M409L p48cre; KrasG12D/+; p53 fl/fl mice N/A Mouse LPCs This paper N/A HPAC ATCC Cat# CRL-2119; RRID: CVCL_3517 AsPC-1 ATCC Cat# CRL-1682; RRID: CVCL_0152 MLE-12 ATCC Cat# CRL-2110, RRID:CVCL_3751 Experimental models: Organisms/strains C57BL/6J Charles River Strain #213 BALB/c Nude Charles River Strain #401 Oligonucleotides Primers for real-time PCR analysis, see Supplemental information This paper N/A Primers for CHIP PCR analysis, see Supplemental information This paper N/A Human Aldh1a3 shRNA, see Supplemental information This paper N/A Mouse Plxnb1 shRNA, see Supplemental information This paper N/A Mouse Nrp1 shRNA, see Supplemental information This paper N/A Mouse Sema3a siRNA, see Supplemental information This paper N/A Recombinant DNA Mouse Aldh1a3 Plasmid Origene Cat# MG222097 Mouse Plxnb1 Plasmid Origene Cat# MR222673 Mouse Nrp1 Plasmid Origene Cat# MR211197 pCMV6-Entry Plasmid Origene Cat# PS100001 Mouse Aldh1a3 CRISPR Plasmid Santa Cruz Biotechnology Cat# sc-425322 Software and algorithms GraphPad Prism software GraphPad Software https://www.graphpad.com/ Bowtie2 SourceForge https://bowtie-bio.sourceforge.net/bowtie2 HISAT Center for Computational Biology at Johns Hopkins University https://ccb.jhu.edu/software/hisat ImageJ NIH Image https://imagej.net/imagej-wiki-static/ImageJ DESeq2 Bioconductor https://bioconductor.org/packages/release/ bioc/html/DESeq2.html R statistical software The R Project https://www.r-project.org (Continued on next page) e2 iScience 29, 115950, June 19, 2026
CRISPR:Article Title: ALDH1A3 promotes the lung metastatic organotropism of pancreatic ductal adenocarcinoma through semaphorin signaling.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Magna ChIP A/G Chromatin Immunoprecipitation Kit Merck Cat# 17-10085 Nuclear and cytoplasmic protein extraction kit Beyotime Cat# P0027 Deposited data RNA Sequencing data This paper GEOID: GSE248375 RNA Sequencing data This paper HRA011000 Multi-omic profiling of lung and liver tumor microenvironments of metastatic pancreatic cancer reveals site-specific immuneregulatory features NCBI Gene Expression Omnibus GEOID: GSE172104 Experimental models: Cell lines Mouse PDAC cell lines: 399, 403, 445,897,907,908,928, 952 and 5M409L p48cre; KrasG12D/+; Tsc1fl/+ mice N/A Mouse PDAC cell lines 5M409L p48cre; KrasG12D/+; p53 fl/fl mice N/A Mouse LPCs This paper N/A HPAC ATCC Cat# CRL-2119; RRID: CVCL_3517 AsPC-1 ATCC Cat# CRL-1682; RRID: CVCL_0152 MLE-12 ATCC Cat# CRL-2110, RRID:CVCL_3751 Experimental models: Organisms/strains C57BL/6J Charles River Strain #213 BALB/c Nude Charles River Strain #401 Oligonucleotides Primers for real-time PCR analysis, see Supplemental information This paper N/A Primers for CHIP PCR analysis, see Supplemental information This paper N/A Human Aldh1a3 shRNA, see Supplemental information This paper N/A Mouse Plxnb1 shRNA, see Supplemental information This paper N/A Mouse Nrp1 shRNA, see Supplemental information This paper N/A Mouse Sema3a siRNA, see Supplemental information This paper N/A Recombinant DNA Mouse Aldh1a3 Plasmid Origene Cat# MG222097 Mouse Plxnb1 Plasmid Origene Cat# MR222673 Mouse Nrp1 Plasmid Origene Cat# MR211197 pCMV6-Entry Plasmid Origene Cat# PS100001 Mouse Aldh1a3 CRISPR Plasmid Santa Cruz Biotechnology Cat# sc-425322 Software and algorithms GraphPad Prism software GraphPad Software https://www.graphpad.com/ Bowtie2 SourceForge https://bowtie-bio.sourceforge.net/bowtie2 HISAT Center for Computational Biology at Johns Hopkins University https://ccb.jhu.edu/software/hisat ImageJ NIH Image https://imagej.net/imagej-wiki-static/ImageJ DESeq2 Bioconductor https://bioconductor.org/packages/release/ bioc/html/DESeq2.html R statistical software The R Project https://www.r-project.org (Continued on next page) e2 iScience 29, 115950, June 19, 2026
Software:Article Title: ALDH1A3 promotes the lung metastatic organotropism of pancreatic ductal adenocarcinoma through semaphorin signaling.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Magna ChIP A/G Chromatin Immunoprecipitation Kit Merck Cat# 17-10085 Nuclear and cytoplasmic protein extraction kit Beyotime Cat# P0027 Deposited data RNA Sequencing data This paper GEOID: GSE248375 RNA Sequencing data This paper HRA011000 Multi-omic profiling of lung and liver tumor microenvironments of metastatic pancreatic cancer reveals site-specific immuneregulatory features NCBI Gene Expression Omnibus GEOID: GSE172104 Experimental models: Cell lines Mouse PDAC cell lines: 399, 403, 445,897,907,908,928, 952 and 5M409L p48cre; KrasG12D/+; Tsc1fl/+ mice N/A Mouse PDAC cell lines 5M409L p48cre; KrasG12D/+; p53 fl/fl mice N/A Mouse LPCs This paper N/A HPAC ATCC Cat# CRL-2119; RRID: CVCL_3517 AsPC-1 ATCC Cat# CRL-1682; RRID: CVCL_0152 MLE-12 ATCC Cat# CRL-2110, RRID:CVCL_3751 Experimental models: Organisms/strains C57BL/6J Charles River Strain #213 BALB/c Nude Charles River Strain #401 Oligonucleotides Primers for real-time PCR analysis, see Supplemental information This paper N/A Primers for CHIP PCR analysis, see Supplemental information This paper N/A Human Aldh1a3 shRNA, see Supplemental information This paper N/A Mouse Plxnb1 shRNA, see Supplemental information This paper N/A Mouse Nrp1 shRNA, see Supplemental information This paper N/A Mouse Sema3a siRNA, see Supplemental information This paper N/A Recombinant DNA Mouse Aldh1a3 Plasmid Origene Cat# MG222097 Mouse Plxnb1 Plasmid Origene Cat# MR222673 Mouse Nrp1 Plasmid Origene Cat# MR211197 pCMV6-Entry Plasmid Origene Cat# PS100001 Mouse Aldh1a3 CRISPR Plasmid Santa Cruz Biotechnology Cat# sc-425322 Software and algorithms GraphPad Prism software GraphPad Software https://www.graphpad.com/ Bowtie2 SourceForge https://bowtie-bio.sourceforge.net/bowtie2 HISAT Center for Computational Biology at Johns Hopkins University https://ccb.jhu.edu/software/hisat ImageJ NIH Image https://imagej.net/imagej-wiki-static/ImageJ DESeq2 Bioconductor https://bioconductor.org/packages/release/ bioc/html/DESeq2.html R statistical software The R Project https://www.r-project.org (Continued on next page) e2 iScience 29, 115950, June 19, 2026
Cell Culture:Article Title: ALDH1A3 promotes the lung metastatic organotropism of pancreatic ductal adenocarcinoma through semaphorin signaling.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Magna ChIP A/G Chromatin Immunoprecipitation Kit Merck Cat# 17-10085 Nuclear and cytoplasmic protein extraction kit Beyotime Cat# P0027 Deposited data RNA Sequencing data This paper GEOID: GSE248375 RNA Sequencing data This paper HRA011000 Multi-omic profiling of lung and liver tumor microenvironments of metastatic pancreatic cancer reveals site-specific immuneregulatory features NCBI Gene Expression Omnibus GEOID: GSE172104 Experimental models: Cell lines Mouse PDAC cell lines: 399, 403, 445,897,907,908,928, 952 and 5M409L p48cre; KrasG12D/+; Tsc1fl/+ mice N/A Mouse PDAC cell lines 5M409L p48cre; KrasG12D/+; p53 fl/fl mice N/A Mouse LPCs This paper N/A HPAC ATCC Cat# CRL-2119; RRID: CVCL_3517 AsPC-1 ATCC Cat# CRL-1682; RRID: CVCL_0152 MLE-12 ATCC Cat# CRL-2110, RRID:CVCL_3751 Experimental models: Organisms/strains C57BL/6J Charles River Strain #213 BALB/c Nude Charles River Strain #401 Oligonucleotides Primers for real-time PCR analysis, see Supplemental information This paper N/A Primers for CHIP PCR analysis, see Supplemental information This paper N/A Human Aldh1a3 shRNA, see Supplemental information This paper N/A Mouse Plxnb1 shRNA, see Supplemental information This paper N/A Mouse Nrp1 shRNA, see Supplemental information This paper N/A Mouse Sema3a siRNA, see Supplemental information This paper N/A Recombinant DNA Mouse Aldh1a3 Plasmid Origene Cat# MG222097 Mouse Plxnb1 Plasmid Origene Cat# MR222673 Mouse Nrp1 Plasmid Origene Cat# MR211197 pCMV6-Entry Plasmid Origene Cat# PS100001 Mouse Aldh1a3 CRISPR Plasmid Santa Cruz Biotechnology Cat# sc-425322 Software and algorithms GraphPad Prism software GraphPad Software https://www.graphpad.com/ Bowtie2 SourceForge https://bowtie-bio.sourceforge.net/bowtie2 HISAT Center for Computational Biology at Johns Hopkins University https://ccb.jhu.edu/software/hisat ImageJ NIH Image https://imagej.net/imagej-wiki-static/ImageJ DESeq2 Bioconductor https://bioconductor.org/packages/release/ bioc/html/DESeq2.html R statistical software The R Project https://www.r-project.org (Continued on next page) e2 iScience 29, 115950, June 19, 2026
Virus:Article Title: ALDH1A3 promotes the lung metastatic organotropism of pancreatic ductal adenocarcinoma through semaphorin signaling.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Magna ChIP A/G Chromatin Immunoprecipitation Kit Merck Cat# 17-10085 Nuclear and cytoplasmic protein extraction kit Beyotime Cat# P0027 Deposited data RNA Sequencing data This paper GEOID: GSE248375 RNA Sequencing data This paper HRA011000 Multi-omic profiling of lung and liver tumor microenvironments of metastatic pancreatic cancer reveals site-specific immuneregulatory features NCBI Gene Expression Omnibus GEOID: GSE172104 Experimental models: Cell lines Mouse PDAC cell lines: 399, 403, 445,897,907,908,928, 952 and 5M409L p48cre; KrasG12D/+; Tsc1fl/+ mice N/A Mouse PDAC cell lines 5M409L p48cre; KrasG12D/+; p53 fl/fl mice N/A Mouse LPCs This paper N/A HPAC ATCC Cat# CRL-2119; RRID: CVCL_3517 AsPC-1 ATCC Cat# CRL-1682; RRID: CVCL_0152 MLE-12 ATCC Cat# CRL-2110, RRID:CVCL_3751 Experimental models: Organisms/strains C57BL/6J Charles River Strain #213 BALB/c Nude Charles River Strain #401 Oligonucleotides Primers for real-time PCR analysis, see Supplemental information This paper N/A Primers for CHIP PCR analysis, see Supplemental information This paper N/A Human Aldh1a3 shRNA, see Supplemental information This paper N/A Mouse Plxnb1 shRNA, see Supplemental information This paper N/A Mouse Nrp1 shRNA, see Supplemental information This paper N/A Mouse Sema3a siRNA, see Supplemental information This paper N/A Recombinant DNA Mouse Aldh1a3 Plasmid Origene Cat# MG222097 Mouse Plxnb1 Plasmid Origene Cat# MR222673 Mouse Nrp1 Plasmid Origene Cat# MR211197 pCMV6-Entry Plasmid Origene Cat# PS100001 Mouse Aldh1a3 CRISPR Plasmid Santa Cruz Biotechnology Cat# sc-425322 Software and algorithms GraphPad Prism software GraphPad Software https://www.graphpad.com/ Bowtie2 SourceForge https://bowtie-bio.sourceforge.net/bowtie2 HISAT Center for Computational Biology at Johns Hopkins University https://ccb.jhu.edu/software/hisat ImageJ NIH Image https://imagej.net/imagej-wiki-static/ImageJ DESeq2 Bioconductor https://bioconductor.org/packages/release/ bioc/html/DESeq2.html R statistical software The R Project https://www.r-project.org (Continued on next page) e2 iScience 29, 115950, June 19, 2026
Microarray:Article Title: ALDH1A3 promotes the lung metastatic organotropism of pancreatic ductal adenocarcinoma through semaphorin signaling.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Magna ChIP A/G Chromatin Immunoprecipitation Kit Merck Cat# 17-10085 Nuclear and cytoplasmic protein extraction kit Beyotime Cat# P0027 Deposited data RNA Sequencing data This paper GEOID: GSE248375 RNA Sequencing data This paper HRA011000 Multi-omic profiling of lung and liver tumor microenvironments of metastatic pancreatic cancer reveals site-specific immuneregulatory features NCBI Gene Expression Omnibus GEOID: GSE172104 Experimental models: Cell lines Mouse PDAC cell lines: 399, 403, 445,897,907,908,928, 952 and 5M409L p48cre; KrasG12D/+; Tsc1fl/+ mice N/A Mouse PDAC cell lines 5M409L p48cre; KrasG12D/+; p53 fl/fl mice N/A Mouse LPCs This paper N/A HPAC ATCC Cat# CRL-2119; RRID: CVCL_3517 AsPC-1 ATCC Cat# CRL-1682; RRID: CVCL_0152 MLE-12 ATCC Cat# CRL-2110, RRID:CVCL_3751 Experimental models: Organisms/strains C57BL/6J Charles River Strain #213 BALB/c Nude Charles River Strain #401 Oligonucleotides Primers for real-time PCR analysis, see Supplemental information This paper N/A Primers for CHIP PCR analysis, see Supplemental information This paper N/A Human Aldh1a3 shRNA, see Supplemental information This paper N/A Mouse Plxnb1 shRNA, see Supplemental information This paper N/A Mouse Nrp1 shRNA, see Supplemental information This paper N/A Mouse Sema3a siRNA, see Supplemental information This paper N/A Recombinant DNA Mouse Aldh1a3 Plasmid Origene Cat# MG222097 Mouse Plxnb1 Plasmid Origene Cat# MR222673 Mouse Nrp1 Plasmid Origene Cat# MR211197 pCMV6-Entry Plasmid Origene Cat# PS100001 Mouse Aldh1a3 CRISPR Plasmid Santa Cruz Biotechnology Cat# sc-425322 Software and algorithms GraphPad Prism software GraphPad Software https://www.graphpad.com/ Bowtie2 SourceForge https://bowtie-bio.sourceforge.net/bowtie2 HISAT Center for Computational Biology at Johns Hopkins University https://ccb.jhu.edu/software/hisat ImageJ NIH Image https://imagej.net/imagej-wiki-static/ImageJ DESeq2 Bioconductor https://bioconductor.org/packages/release/ bioc/html/DESeq2.html R statistical software The R Project https://www.r-project.org (Continued on next page) e2 iScience 29, 115950, June 19, 2026
Labeling:Article Title: ALDH1A3 promotes the lung metastatic organotropism of pancreatic ductal adenocarcinoma through semaphorin signaling.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Magna ChIP A/G Chromatin Immunoprecipitation Kit Merck Cat# 17-10085 Nuclear and cytoplasmic protein extraction kit Beyotime Cat# P0027 Deposited data RNA Sequencing data This paper GEOID: GSE248375 RNA Sequencing data This paper HRA011000 Multi-omic profiling of lung and liver tumor microenvironments of metastatic pancreatic cancer reveals site-specific immuneregulatory features NCBI Gene Expression Omnibus GEOID: GSE172104 Experimental models: Cell lines Mouse PDAC cell lines: 399, 403, 445,897,907,908,928, 952 and 5M409L p48cre; KrasG12D/+; Tsc1fl/+ mice N/A Mouse PDAC cell lines 5M409L p48cre; KrasG12D/+; p53 fl/fl mice N/A Mouse LPCs This paper N/A HPAC ATCC Cat# CRL-2119; RRID: CVCL_3517 AsPC-1 ATCC Cat# CRL-1682; RRID: CVCL_0152 MLE-12 ATCC Cat# CRL-2110, RRID:CVCL_3751 Experimental models: Organisms/strains C57BL/6J Charles River Strain #213 BALB/c Nude Charles River Strain #401 Oligonucleotides Primers for real-time PCR analysis, see Supplemental information This paper N/A Primers for CHIP PCR analysis, see Supplemental information This paper N/A Human Aldh1a3 shRNA, see Supplemental information This paper N/A Mouse Plxnb1 shRNA, see Supplemental information This paper N/A Mouse Nrp1 shRNA, see Supplemental information This paper N/A Mouse Sema3a siRNA, see Supplemental information This paper N/A Recombinant DNA Mouse Aldh1a3 Plasmid Origene Cat# MG222097 Mouse Plxnb1 Plasmid Origene Cat# MR222673 Mouse Nrp1 Plasmid Origene Cat# MR211197 pCMV6-Entry Plasmid Origene Cat# PS100001 Mouse Aldh1a3 CRISPR Plasmid Santa Cruz Biotechnology Cat# sc-425322 Software and algorithms GraphPad Prism software GraphPad Software https://www.graphpad.com/ Bowtie2 SourceForge https://bowtie-bio.sourceforge.net/bowtie2 HISAT Center for Computational Biology at Johns Hopkins University https://ccb.jhu.edu/software/hisat ImageJ NIH Image https://imagej.net/imagej-wiki-static/ImageJ DESeq2 Bioconductor https://bioconductor.org/packages/release/ bioc/html/DESeq2.html R statistical software The R Project https://www.r-project.org (Continued on next page) e2 iScience 29, 115950, June 19, 2026
CCK-8 Assay:Article Title: ALDH1A3 promotes the lung metastatic organotropism of pancreatic ductal adenocarcinoma through semaphorin signaling.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Magna ChIP A/G Chromatin Immunoprecipitation Kit Merck Cat# 17-10085 Nuclear and cytoplasmic protein extraction kit Beyotime Cat# P0027 Deposited data RNA Sequencing data This paper GEOID: GSE248375 RNA Sequencing data This paper HRA011000 Multi-omic profiling of lung and liver tumor microenvironments of metastatic pancreatic cancer reveals site-specific immuneregulatory features NCBI Gene Expression Omnibus GEOID: GSE172104 Experimental models: Cell lines Mouse PDAC cell lines: 399, 403, 445,897,907,908,928, 952 and 5M409L p48cre; KrasG12D/+; Tsc1fl/+ mice N/A Mouse PDAC cell lines 5M409L p48cre; KrasG12D/+; p53 fl/fl mice N/A Mouse LPCs This paper N/A HPAC ATCC Cat# CRL-2119; RRID: CVCL_3517 AsPC-1 ATCC Cat# CRL-1682; RRID: CVCL_0152 MLE-12 ATCC Cat# CRL-2110, RRID:CVCL_3751 Experimental models: Organisms/strains C57BL/6J Charles River Strain #213 BALB/c Nude Charles River Strain #401 Oligonucleotides Primers for real-time PCR analysis, see Supplemental information This paper N/A Primers for CHIP PCR analysis, see Supplemental information This paper N/A Human Aldh1a3 shRNA, see Supplemental information This paper N/A Mouse Plxnb1 shRNA, see Supplemental information This paper N/A Mouse Nrp1 shRNA, see Supplemental information This paper N/A Mouse Sema3a siRNA, see Supplemental information This paper N/A Recombinant DNA Mouse Aldh1a3 Plasmid Origene Cat# MG222097 Mouse Plxnb1 Plasmid Origene Cat# MR222673 Mouse Nrp1 Plasmid Origene Cat# MR211197 pCMV6-Entry Plasmid Origene Cat# PS100001 Mouse Aldh1a3 CRISPR Plasmid Santa Cruz Biotechnology Cat# sc-425322 Software and algorithms GraphPad Prism software GraphPad Software https://www.graphpad.com/ Bowtie2 SourceForge https://bowtie-bio.sourceforge.net/bowtie2 HISAT Center for Computational Biology at Johns Hopkins University https://ccb.jhu.edu/software/hisat ImageJ NIH Image https://imagej.net/imagej-wiki-static/ImageJ DESeq2 Bioconductor https://bioconductor.org/packages/release/ bioc/html/DESeq2.html R statistical software The R Project https://www.r-project.org (Continued on next page) e2 iScience 29, 115950, June 19, 2026
Luciferase:Article Title: ALDH1A3 promotes the lung metastatic organotropism of pancreatic ductal adenocarcinoma through semaphorin signaling.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Magna ChIP A/G Chromatin Immunoprecipitation Kit Merck Cat# 17-10085 Nuclear and cytoplasmic protein extraction kit Beyotime Cat# P0027 Deposited data RNA Sequencing data This paper GEOID: GSE248375 RNA Sequencing data This paper HRA011000 Multi-omic profiling of lung and liver tumor microenvironments of metastatic pancreatic cancer reveals site-specific immuneregulatory features NCBI Gene Expression Omnibus GEOID: GSE172104 Experimental models: Cell lines Mouse PDAC cell lines: 399, 403, 445,897,907,908,928, 952 and 5M409L p48cre; KrasG12D/+; Tsc1fl/+ mice N/A Mouse PDAC cell lines 5M409L p48cre; KrasG12D/+; p53 fl/fl mice N/A Mouse LPCs This paper N/A HPAC ATCC Cat# CRL-2119; RRID: CVCL_3517 AsPC-1 ATCC Cat# CRL-1682; RRID: CVCL_0152 MLE-12 ATCC Cat# CRL-2110, RRID:CVCL_3751 Experimental models: Organisms/strains C57BL/6J Charles River Strain #213 BALB/c Nude Charles River Strain #401 Oligonucleotides Primers for real-time PCR analysis, see Supplemental information This paper N/A Primers for CHIP PCR analysis, see Supplemental information This paper N/A Human Aldh1a3 shRNA, see Supplemental information This paper N/A Mouse Plxnb1 shRNA, see Supplemental information This paper N/A Mouse Nrp1 shRNA, see Supplemental information This paper N/A Mouse Sema3a siRNA, see Supplemental information This paper N/A Recombinant DNA Mouse Aldh1a3 Plasmid Origene Cat# MG222097 Mouse Plxnb1 Plasmid Origene Cat# MR222673 Mouse Nrp1 Plasmid Origene Cat# MR211197 pCMV6-Entry Plasmid Origene Cat# PS100001 Mouse Aldh1a3 CRISPR Plasmid Santa Cruz Biotechnology Cat# sc-425322 Software and algorithms GraphPad Prism software GraphPad Software https://www.graphpad.com/ Bowtie2 SourceForge https://bowtie-bio.sourceforge.net/bowtie2 HISAT Center for Computational Biology at Johns Hopkins University https://ccb.jhu.edu/software/hisat ImageJ NIH Image https://imagej.net/imagej-wiki-static/ImageJ DESeq2 Bioconductor https://bioconductor.org/packages/release/ bioc/html/DESeq2.html R statistical software The R Project https://www.r-project.org (Continued on next page) e2 iScience 29, 115950, June 19, 2026
Multiple Displacement Amplification:Article Title: ALDH1A3 promotes the lung metastatic organotropism of pancreatic ductal adenocarcinoma through semaphorin signaling.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Magna ChIP A/G Chromatin Immunoprecipitation Kit Merck Cat# 17-10085 Nuclear and cytoplasmic protein extraction kit Beyotime Cat# P0027 Deposited data RNA Sequencing data This paper GEOID: GSE248375 RNA Sequencing data This paper HRA011000 Multi-omic profiling of lung and liver tumor microenvironments of metastatic pancreatic cancer reveals site-specific immuneregulatory features NCBI Gene Expression Omnibus GEOID: GSE172104 Experimental models: Cell lines Mouse PDAC cell lines: 399, 403, 445,897,907,908,928, 952 and 5M409L p48cre; KrasG12D/+; Tsc1fl/+ mice N/A Mouse PDAC cell lines 5M409L p48cre; KrasG12D/+; p53 fl/fl mice N/A Mouse LPCs This paper N/A HPAC ATCC Cat# CRL-2119; RRID: CVCL_3517 AsPC-1 ATCC Cat# CRL-1682; RRID: CVCL_0152 MLE-12 ATCC Cat# CRL-2110, RRID:CVCL_3751 Experimental models: Organisms/strains C57BL/6J Charles River Strain #213 BALB/c Nude Charles River Strain #401 Oligonucleotides Primers for real-time PCR analysis, see Supplemental information This paper N/A Primers for CHIP PCR analysis, see Supplemental information This paper N/A Human Aldh1a3 shRNA, see Supplemental information This paper N/A Mouse Plxnb1 shRNA, see Supplemental information This paper N/A Mouse Nrp1 shRNA, see Supplemental information This paper N/A Mouse Sema3a siRNA, see Supplemental information This paper N/A Recombinant DNA Mouse Aldh1a3 Plasmid Origene Cat# MG222097 Mouse Plxnb1 Plasmid Origene Cat# MR222673 Mouse Nrp1 Plasmid Origene Cat# MR211197 pCMV6-Entry Plasmid Origene Cat# PS100001 Mouse Aldh1a3 CRISPR Plasmid Santa Cruz Biotechnology Cat# sc-425322 Software and algorithms GraphPad Prism software GraphPad Software https://www.graphpad.com/ Bowtie2 SourceForge https://bowtie-bio.sourceforge.net/bowtie2 HISAT Center for Computational Biology at Johns Hopkins University https://ccb.jhu.edu/software/hisat ImageJ NIH Image https://imagej.net/imagej-wiki-static/ImageJ DESeq2 Bioconductor https://bioconductor.org/packages/release/ bioc/html/DESeq2.html R statistical software The R Project https://www.r-project.org (Continued on next page) e2 iScience 29, 115950, June 19, 2026
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