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Matrix Science mascot 2.4 algorithm
Mascot 2.4 Algorithm, supplied by Matrix Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mascot+2%2E4+algorithm/mascot+algorithm/pm38553595-299-31-34
Average 90 stars, based on 1 article reviews
mascot 2.4 algorithm - by Bioz Stars, 2026-11
90/100 stars

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Article Title: Biochemical and functional properties of vesicles from planktonic and biofilm phenotypes of Limosilactobacillus reuteri DSM 17938.
Article Snippet: All MS and MS/MS raw data files per sample were merged for protein identification and relative protein quantification into ProteomeDiscoverer vs. 2.4 software (Thermo Scientific), enabling the database search by Mascot algorithm v. 2.4.2 (Matrix Science, UK) using the following criteria: L. reuteri UniProtKB protein database (72,034 protein sequences, 06/2022) including the most common protein contaminants; carbamidomethylation of Cys and TMT6plex modification of lysine and peptide N-terminus as fixed modifications; oxidation at Met, deamidation at Asn and Gln, pyroglutamate formation at Gln as variable modifications.

Article Title: Subversion of phosphorylated SR proteins by enterovirus A71 in IRES-dependent translation revealed by RNA-interactome analysis.
Article Snippet: Subsequently, the samples were separated by 8%, 10%, or 12% SDS-polyacrylamide gel electrophoresis (SDS-PAGE) based on the MW of the proteins of interest and transferred to a PVDF membrane (Cytiva, Marlborough, MA, USA).

Article Title: Coupling tRNA Gly gene redundancy with staphylococcal cell wall integrity, antibiotic susceptibility, and virulence potential
Article Snippet: Peptides were separated on the integrated emitter column IonOpticks Aurora Elite (25 cm × 75 μM, 1.7 μm particle size, and 120 Å pore size; AUR3-15075C18-CSI).

Article Title: Identification of uranyl-binding proteins in Arabidopsis thaliana cells exposed to uranium: Insights from a metalloproteomic analysis and characterization of Glycine-Rich RNA-binding protein 7 (GRP7).
Article Snippet: For protein identification, the MS/MS data were interpreted using a local Mascot server with MASCOT 2.6.2 algorithm (Matrix Science, London, UK) against UniProtKB/SwissProt database (version 2019_09 561,176 sequences and version 2019_10 561,356 sequences).

Article Title: CLEC-1 is a death sensor that limits antigen cross-presentation by dendritic cells and represents a target for cancer immunotherapy
Article Snippet: Eluate was revealed by WB with anti-human TRIM21 mAbs (AB01/1G5, Biorad).

Sequencing:

Article Title: Subversion of phosphorylated SR proteins by enterovirus A71 in IRES-dependent translation revealed by RNA-interactome analysis
Article Snippet: The MS data have been deposited in ProteomeXchange with the identifier PXD036890 [ ]. .. To identify peptides, mass spectra were analyzed using the Mascot algorithm (Version 2.1, Matrix Science, Boston, MA, USA) and searched against the SwissProt human sequence database (released March 2018, selected for Homo sapiens, 20,198 entries) from the European Bioinformatics Institute. .. The search was conducted using the Proteome Discoverer software (version 2.1, Thermo Fisher Scientific).

Article Title: Identification of α2-macroglobulin as a biomarker for type 2 diabetes in human serum
Article Snippet: .. The Mascot algorithm (Matrix Science, Boston, MA, USA) was utilized to identify peptide sequences in the protein sequence database. ..



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