p-mtor (s2448; o21-404) (Becton Dickinson)
90
Structured Review
Becton Dickinson
p-mtor (s2448; o21-404)
P Mtor (S2448; O21 404), supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mapix%2C+data+404+acquisition+and+microarray+image+analysis+software/p+mtor++ser2448++antibody/pmc06371971-23-0-4
Average 90 stars, based on 1 article reviews
P Mtor (S2448; O21 404), supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mapix%2C+data+404+acquisition+and+microarray+image+analysis+software/p+mtor++ser2448++antibody/pmc06371971-23-0-4
Average 90 stars, based on 1 article reviews
p-mtor (s2448; o21-404) - by Bioz Stars,
2026-10
90/100 stars
Images
1) Product Images from "Activation of miR-21-Regulated Pathways in Immune Aging Selects against Signatures Characteristic of Memory T Cells"
Article Title: Activation of miR-21-Regulated Pathways in Immune Aging Selects against Signatures Characteristic of Memory T Cells
Journal: Cell reports
doi: 10.1016/j.celrep.2018.10.074
Figure Legend Snippet: (A) Naive CD4 + T cells were transfected with either scrambled control or LNA21. Representative western blots and mean normalized band intensities of PTEN, SPRY1, and b-actin expression after 48 hr are shown (n = 3–4, mean ± SEM). (B and C) Naive CD4 + T cells from young and older individuals were activated with anti-CD3 and anti-CD28 beads and transduced with a lentiviral vector expressing scrambled control RNA or anti-miR-21. (B) Representative histogram of phosphorylated S6 in GFP + cells on day 2 and results from 7 individuals. (C) Representative histograms of phosphorylated S6, AKT, mTOR, and ERK in GFP + cells on day 3 and results of paired samples from 7 individuals. The filled gray histograms represent unstimulated naive CD4 + T cells. (D and E) Naive CD4 + T cells from healthy adults were activated with anti-CD3 and anti-CD28 beads. On day 3, activated cells were treated with combinations of the AKT inhibitor MK-2206 2HCl and the MEK1 and MEK2 inhibitor U0126 for 1.5 hr. (D) Representative histograms show phosphorylated S6, mTOR, and ERK in cells treated with combinations of 40 nM of the AKT inhibitor and 400 nM of MEK1 and MEK2 inhibitor. Numbers in histograms indicate geometric MFI of p-mTOR and p-ERK. (E) Graphs depicting the frequencies of cells with phosphorylated S6 cultured with the indicated concentrations of the AKT inhibitor in the presence or absence of 400 nM of the MEK1 and MEK2 inhibitor (n = 4, mean ± SEM). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; all by two-tailed paired t test.
Techniques Used: Transfection, Western Blot, Expressing, Transduction, Plasmid Preparation, Cell Culture, Two Tailed Test
Figure Legend Snippet:
Techniques Used: Plasmid Preparation, Recombinant, Isolation, SYBR Green Assay, Reporter Assay, Microarray, Luciferase, Software
Related Articles
Staining:Article Title: IL-21, inflammatory cytokines and hyper polarized CD8 + T cells are central players in Lupus immune pathology Article Snippet: Then they were permeabilised with ice cold methanol with vigorous vortexing and incubated at 4° C for 15mins.After washing with staining media cells were stained with p-mTOR (Ser2448) Antibody for 30 mins at room temperature and then acquired on BD LSR Fortessa[ , ]. Article Title: Functional characterization of the PI3K/AKT/MTOR signaling pathway for targeted therapy in B-precursor acute lymphoblastic leukemia. Article Snippet: Alexa 647 p-MTOR (Ser2448) (clone O21-404) and PE p-AKT (Ser473) (clone M89-61) for Article Title: Amniotic MSC affect CD8 naive polarization toward SLEC/MPEC subsets by down-modulating IL-12Rβ1 and IL-2Rα signaling pathways Article Snippet: Finally, cells were stained for 1h at RT with BD PhosflowTM (BD Bioscience) fluorescently conjugated antibodies against: Stat4 (pY693) Alexa fluor® 488 (clone 38/p-Stat4), Stat5 (pY694) PE-Cy7 (clone 47/Stat5(pY694)), Anti-Akt (pS473) PE-CF594 (clone M89-61), Anti-mTOR (pS2448) Alexa fluor® 647 (clone 021-404) all purchased from BD Biosciences. Article Title: IL-21, Inflammatory Cytokines and Hyperpolarized CD8 + T Cells Are Central Players in Lupus Immune Pathology Article Snippet: After washing with staining media, cells were stained with p-mTOR (Ser2448) Antibody for 30 min at room temperature and then acquired on a BD LSR Fortessa [ , ]. Article Title: Characterization of the bone marrow niche in patients with chronic myeloid leukemia identifies CXCL14 as a new therapeutic option Article Snippet: After spinning down at 800 x g 5 min, the cells were stained with BD Phosflow Alexa Fluor 647 mouse anti-mTOR (pS2448), cat#564242) or purified primary antibodies against OPA3 (ab230205), CYC1 (ab224044), SLC25A26 [EPR11581] (ab175209) and SERCA2 ATPase [EPR9392] (ab150435) in BD Perm/Wash buffer (BD 554723) overnight at 4°C. Article Title: A tumor microenvironment model of chronic lymphocytic leukemia enables drug sensitivity testing to guide precision medicine. Article Snippet: Antibodies against Bcl-2 (pS70) (N46-467), Btk (pY223)/Itk (pY180) (N35-86), Btk (pY551) & p-Itk (pY511) (24a/BTK (Y551), IgG1 Kappa (MOPC-21), MEK1 (pS298) (J114-64), MEK1 (pS218)/MEK2 (pS222) (024-836), mTOR (pS2448) (O21-404), NF-κB p65 (pS529) (K10895.12.50), p53 (pS37) (J159-641.79), PLCγ2 (pY759) (K86-689.37), Rb (pS807/S811) (J112-906), STAT1 (pS272) (K51-856), STAT1 (pY701) (14/P), STAT3 (pS727) (49/p-Stat3), STAT3 (pY705) (4/p-STAT3), STAT5 (pY694) (47), STAT6 (pY614) (18/p-Stat6), TBK1 (pS172) (J133-587), and Article Title: IL-21, Inflammatory Cytokines and Hyperpolarized CD8 + T Cells Are Central Players in Lupus Immune Pathology. Article Snippet: After washing with staining media, cells were stained with p-mTOR (Ser2448) Antibody for 30 min at room temperature and then acquired on a BD LSR Fortessa [30,32]. Article Title: Amniotic MSC affect CD8 naive polarization toward SLEC/MPEC subsets by down-modulating IL-12Rβ1 and IL-2Rα signaling pathways Article Snippet: Plasmid Preparation:Article Title: IL-21, inflammatory cytokines and hyper polarized CD8 + T cells are central players in Lupus immune pathology Article Snippet: Then they were permeabilised with ice cold methanol with vigorous vortexing and incubated at 4° C for 15mins.After washing with staining media cells were stained with p-mTOR (Ser2448) Antibody for 30 mins at room temperature and then acquired on BD LSR Fortessa[ , ]. Article Title: Functional characterization of the PI3K/AKT/MTOR signaling pathway for targeted therapy in B-precursor acute lymphoblastic leukemia. Article Snippet: Alexa 647 p-MTOR (Ser2448) (clone O21-404) and PE p-AKT (Ser473) (clone M89-61) for Article Title: Amniotic MSC affect CD8 naive polarization toward SLEC/MPEC subsets by down-modulating IL-12Rβ1 and IL-2Rα signaling pathways Article Snippet: Finally, cells were stained for 1h at RT with BD PhosflowTM (BD Bioscience) fluorescently conjugated antibodies against: Stat4 (pY693) Alexa fluor® 488 (clone 38/p-Stat4), Stat5 (pY694) PE-Cy7 (clone 47/Stat5(pY694)), Anti-Akt (pS473) PE-CF594 (clone M89-61), Anti-mTOR (pS2448) Alexa fluor® 647 (clone 021-404) all purchased from BD Biosciences. Article Title: IL-21, Inflammatory Cytokines and Hyperpolarized CD8 + T Cells Are Central Players in Lupus Immune Pathology Article Snippet: After washing with staining media, cells were stained with p-mTOR (Ser2448) Antibody for 30 min at room temperature and then acquired on a BD LSR Fortessa [ , ]. Article Title: Characterization of the bone marrow niche in patients with chronic myeloid leukemia identifies CXCL14 as a new therapeutic option Article Snippet: After spinning down at 800 x g 5 min, the cells were stained with BD Phosflow Alexa Fluor 647 mouse anti-mTOR (pS2448), cat#564242) or purified primary antibodies against OPA3 (ab230205), CYC1 (ab224044), SLC25A26 [EPR11581] (ab175209) and SERCA2 ATPase [EPR9392] (ab150435) in BD Perm/Wash buffer (BD 554723) overnight at 4°C. Article Title: A tumor microenvironment model of chronic lymphocytic leukemia enables drug sensitivity testing to guide precision medicine. Article Snippet: Antibodies against Bcl-2 (pS70) (N46-467), Btk (pY223)/Itk (pY180) (N35-86), Btk (pY551) & p-Itk (pY511) (24a/BTK (Y551), IgG1 Kappa (MOPC-21), MEK1 (pS298) (J114-64), MEK1 (pS218)/MEK2 (pS222) (024-836), mTOR (pS2448) (O21-404), NF-κB p65 (pS529) (K10895.12.50), p53 (pS37) (J159-641.79), PLCγ2 (pY759) (K86-689.37), Rb (pS807/S811) (J112-906), STAT1 (pS272) (K51-856), STAT1 (pY701) (14/P), STAT3 (pS727) (49/p-Stat3), STAT3 (pY705) (4/p-STAT3), STAT5 (pY694) (47), STAT6 (pY614) (18/p-Stat6), TBK1 (pS172) (J133-587), and Article Title: IL-21, Inflammatory Cytokines and Hyperpolarized CD8 + T Cells Are Central Players in Lupus Immune Pathology. Article Snippet: After washing with staining media, cells were stained with p-mTOR (Ser2448) Antibody for 30 min at room temperature and then acquired on a BD LSR Fortessa [30,32]. Article Title: Amniotic MSC affect CD8 naive polarization toward SLEC/MPEC subsets by down-modulating IL-12Rβ1 and IL-2Rα signaling pathways Article Snippet: Recombinant:Article Title: IL-21, inflammatory cytokines and hyper polarized CD8 + T cells are central players in Lupus immune pathology Article Snippet: Then they were permeabilised with ice cold methanol with vigorous vortexing and incubated at 4° C for 15mins.After washing with staining media cells were stained with p-mTOR (Ser2448) Antibody for 30 mins at room temperature and then acquired on BD LSR Fortessa[ , ]. Article Title: Functional characterization of the PI3K/AKT/MTOR signaling pathway for targeted therapy in B-precursor acute lymphoblastic leukemia. Article Snippet: Alexa 647 p-MTOR (Ser2448) (clone O21-404) and PE p-AKT (Ser473) (clone M89-61) for Article Title: Amniotic MSC affect CD8 naive polarization toward SLEC/MPEC subsets by down-modulating IL-12Rβ1 and IL-2Rα signaling pathways Article Snippet: Finally, cells were stained for 1h at RT with BD PhosflowTM (BD Bioscience) fluorescently conjugated antibodies against: Stat4 (pY693) Alexa fluor® 488 (clone 38/p-Stat4), Stat5 (pY694) PE-Cy7 (clone 47/Stat5(pY694)), Anti-Akt (pS473) PE-CF594 (clone M89-61), Anti-mTOR (pS2448) Alexa fluor® 647 (clone 021-404) all purchased from BD Biosciences. Article Title: IL-21, Inflammatory Cytokines and Hyperpolarized CD8 + T Cells Are Central Players in Lupus Immune Pathology Article Snippet: After washing with staining media, cells were stained with p-mTOR (Ser2448) Antibody for 30 min at room temperature and then acquired on a BD LSR Fortessa [ , ]. Article Title: Characterization of the bone marrow niche in patients with chronic myeloid leukemia identifies CXCL14 as a new therapeutic option Article Snippet: After spinning down at 800 x g 5 min, the cells were stained with BD Phosflow Alexa Fluor 647 mouse anti-mTOR (pS2448), cat#564242) or purified primary antibodies against OPA3 (ab230205), CYC1 (ab224044), SLC25A26 [EPR11581] (ab175209) and SERCA2 ATPase [EPR9392] (ab150435) in BD Perm/Wash buffer (BD 554723) overnight at 4°C. Article Title: A tumor microenvironment model of chronic lymphocytic leukemia enables drug sensitivity testing to guide precision medicine. Article Snippet: Antibodies against Bcl-2 (pS70) (N46-467), Btk (pY223)/Itk (pY180) (N35-86), Btk (pY551) & p-Itk (pY511) (24a/BTK (Y551), IgG1 Kappa (MOPC-21), MEK1 (pS298) (J114-64), MEK1 (pS218)/MEK2 (pS222) (024-836), mTOR (pS2448) (O21-404), NF-κB p65 (pS529) (K10895.12.50), p53 (pS37) (J159-641.79), PLCγ2 (pY759) (K86-689.37), Rb (pS807/S811) (J112-906), STAT1 (pS272) (K51-856), STAT1 (pY701) (14/P), STAT3 (pS727) (49/p-Stat3), STAT3 (pY705) (4/p-STAT3), STAT5 (pY694) (47), STAT6 (pY614) (18/p-Stat6), TBK1 (pS172) (J133-587), and Article Title: IL-21, Inflammatory Cytokines and Hyperpolarized CD8 + T Cells Are Central Players in Lupus Immune Pathology. Article Snippet: After washing with staining media, cells were stained with p-mTOR (Ser2448) Antibody for 30 min at room temperature and then acquired on a BD LSR Fortessa [30,32]. Article Title: Amniotic MSC affect CD8 naive polarization toward SLEC/MPEC subsets by down-modulating IL-12Rβ1 and IL-2Rα signaling pathways Article Snippet: Software:Article Title: IL-21, inflammatory cytokines and hyper polarized CD8 + T cells are central players in Lupus immune pathology Article Snippet: Then they were permeabilised with ice cold methanol with vigorous vortexing and incubated at 4° C for 15mins.After washing with staining media cells were stained with p-mTOR (Ser2448) Antibody for 30 mins at room temperature and then acquired on BD LSR Fortessa[ , ]. Article Title: Functional characterization of the PI3K/AKT/MTOR signaling pathway for targeted therapy in B-precursor acute lymphoblastic leukemia. Article Snippet: Alexa 647 p-MTOR (Ser2448) (clone O21-404) and PE p-AKT (Ser473) (clone M89-61) for Article Title: Amniotic MSC affect CD8 naive polarization toward SLEC/MPEC subsets by down-modulating IL-12Rβ1 and IL-2Rα signaling pathways Article Snippet: Finally, cells were stained for 1h at RT with BD PhosflowTM (BD Bioscience) fluorescently conjugated antibodies against: Stat4 (pY693) Alexa fluor® 488 (clone 38/p-Stat4), Stat5 (pY694) PE-Cy7 (clone 47/Stat5(pY694)), Anti-Akt (pS473) PE-CF594 (clone M89-61), Anti-mTOR (pS2448) Alexa fluor® 647 (clone 021-404) all purchased from BD Biosciences. Article Title: IL-21, Inflammatory Cytokines and Hyperpolarized CD8 + T Cells Are Central Players in Lupus Immune Pathology Article Snippet: After washing with staining media, cells were stained with p-mTOR (Ser2448) Antibody for 30 min at room temperature and then acquired on a BD LSR Fortessa [ , ]. Article Title: Characterization of the bone marrow niche in patients with chronic myeloid leukemia identifies CXCL14 as a new therapeutic option Article Snippet: After spinning down at 800 x g 5 min, the cells were stained with BD Phosflow Alexa Fluor 647 mouse anti-mTOR (pS2448), cat#564242) or purified primary antibodies against OPA3 (ab230205), CYC1 (ab224044), SLC25A26 [EPR11581] (ab175209) and SERCA2 ATPase [EPR9392] (ab150435) in BD Perm/Wash buffer (BD 554723) overnight at 4°C. Article Title: A tumor microenvironment model of chronic lymphocytic leukemia enables drug sensitivity testing to guide precision medicine. Article Snippet: Antibodies against Bcl-2 (pS70) (N46-467), Btk (pY223)/Itk (pY180) (N35-86), Btk (pY551) & p-Itk (pY511) (24a/BTK (Y551), IgG1 Kappa (MOPC-21), MEK1 (pS298) (J114-64), MEK1 (pS218)/MEK2 (pS222) (024-836), mTOR (pS2448) (O21-404), NF-κB p65 (pS529) (K10895.12.50), p53 (pS37) (J159-641.79), PLCγ2 (pY759) (K86-689.37), Rb (pS807/S811) (J112-906), STAT1 (pS272) (K51-856), STAT1 (pY701) (14/P), STAT3 (pS727) (49/p-Stat3), STAT3 (pY705) (4/p-STAT3), STAT5 (pY694) (47), STAT6 (pY614) (18/p-Stat6), TBK1 (pS172) (J133-587), and Article Title: IL-21, Inflammatory Cytokines and Hyperpolarized CD8 + T Cells Are Central Players in Lupus Immune Pathology. Article Snippet: After washing with staining media, cells were stained with p-mTOR (Ser2448) Antibody for 30 min at room temperature and then acquired on a BD LSR Fortessa [30,32]. Article Title: Amniotic MSC affect CD8 naive polarization toward SLEC/MPEC subsets by down-modulating IL-12Rβ1 and IL-2Rα signaling pathways Article Snippet: SYBR Green Assay:Article Title: IL-21, inflammatory cytokines and hyper polarized CD8 + T cells are central players in Lupus immune pathology Article Snippet: Then they were permeabilised with ice cold methanol with vigorous vortexing and incubated at 4° C for 15mins.After washing with staining media cells were stained with p-mTOR (Ser2448) Antibody for 30 mins at room temperature and then acquired on BD LSR Fortessa[ , ]. Article Title: Functional characterization of the PI3K/AKT/MTOR signaling pathway for targeted therapy in B-precursor acute lymphoblastic leukemia. Article Snippet: Alexa 647 p-MTOR (Ser2448) (clone O21-404) and PE p-AKT (Ser473) (clone M89-61) for Article Title: Amniotic MSC affect CD8 naive polarization toward SLEC/MPEC subsets by down-modulating IL-12Rβ1 and IL-2Rα signaling pathways Article Snippet: Finally, cells were stained for 1h at RT with BD PhosflowTM (BD Bioscience) fluorescently conjugated antibodies against: Stat4 (pY693) Alexa fluor® 488 (clone 38/p-Stat4), Stat5 (pY694) PE-Cy7 (clone 47/Stat5(pY694)), Anti-Akt (pS473) PE-CF594 (clone M89-61), Anti-mTOR (pS2448) Alexa fluor® 647 (clone 021-404) all purchased from BD Biosciences. Article Title: IL-21, Inflammatory Cytokines and Hyperpolarized CD8 + T Cells Are Central Players in Lupus Immune Pathology Article Snippet: After washing with staining media, cells were stained with p-mTOR (Ser2448) Antibody for 30 min at room temperature and then acquired on a BD LSR Fortessa [ , ]. Article Title: Characterization of the bone marrow niche in patients with chronic myeloid leukemia identifies CXCL14 as a new therapeutic option Article Snippet: After spinning down at 800 x g 5 min, the cells were stained with BD Phosflow Alexa Fluor 647 mouse anti-mTOR (pS2448), cat#564242) or purified primary antibodies against OPA3 (ab230205), CYC1 (ab224044), SLC25A26 [EPR11581] (ab175209) and SERCA2 ATPase [EPR9392] (ab150435) in BD Perm/Wash buffer (BD 554723) overnight at 4°C. Article Title: A tumor microenvironment model of chronic lymphocytic leukemia enables drug sensitivity testing to guide precision medicine. Article Snippet: Antibodies against Bcl-2 (pS70) (N46-467), Btk (pY223)/Itk (pY180) (N35-86), Btk (pY551) & p-Itk (pY511) (24a/BTK (Y551), IgG1 Kappa (MOPC-21), MEK1 (pS298) (J114-64), MEK1 (pS218)/MEK2 (pS222) (024-836), mTOR (pS2448) (O21-404), NF-κB p65 (pS529) (K10895.12.50), p53 (pS37) (J159-641.79), PLCγ2 (pY759) (K86-689.37), Rb (pS807/S811) (J112-906), STAT1 (pS272) (K51-856), STAT1 (pY701) (14/P), STAT3 (pS727) (49/p-Stat3), STAT3 (pY705) (4/p-STAT3), STAT5 (pY694) (47), STAT6 (pY614) (18/p-Stat6), TBK1 (pS172) (J133-587), and Article Title: IL-21, Inflammatory Cytokines and Hyperpolarized CD8 + T Cells Are Central Players in Lupus Immune Pathology. Article Snippet: After washing with staining media, cells were stained with p-mTOR (Ser2448) Antibody for 30 min at room temperature and then acquired on a BD LSR Fortessa [30,32]. Article Title: Amniotic MSC affect CD8 naive polarization toward SLEC/MPEC subsets by down-modulating IL-12Rβ1 and IL-2Rα signaling pathways Article Snippet: Cell Isolation:Article Title: IL-21, inflammatory cytokines and hyper polarized CD8 + T cells are central players in Lupus immune pathology Article Snippet: Then they were permeabilised with ice cold methanol with vigorous vortexing and incubated at 4° C for 15mins.After washing with staining media cells were stained with p-mTOR (Ser2448) Antibody for 30 mins at room temperature and then acquired on BD LSR Fortessa[ , ]. Article Title: Functional characterization of the PI3K/AKT/MTOR signaling pathway for targeted therapy in B-precursor acute lymphoblastic leukemia. Article Snippet: Alexa 647 p-MTOR (Ser2448) (clone O21-404) and PE p-AKT (Ser473) (clone M89-61) for Article Title: Amniotic MSC affect CD8 naive polarization toward SLEC/MPEC subsets by down-modulating IL-12Rβ1 and IL-2Rα signaling pathways Article Snippet: Finally, cells were stained for 1h at RT with BD PhosflowTM (BD Bioscience) fluorescently conjugated antibodies against: Stat4 (pY693) Alexa fluor® 488 (clone 38/p-Stat4), Stat5 (pY694) PE-Cy7 (clone 47/Stat5(pY694)), Anti-Akt (pS473) PE-CF594 (clone M89-61), Anti-mTOR (pS2448) Alexa fluor® 647 (clone 021-404) all purchased from BD Biosciences. Article Title: IL-21, Inflammatory Cytokines and Hyperpolarized CD8 + T Cells Are Central Players in Lupus Immune Pathology Article Snippet: After washing with staining media, cells were stained with p-mTOR (Ser2448) Antibody for 30 min at room temperature and then acquired on a BD LSR Fortessa [ , ]. Article Title: Characterization of the bone marrow niche in patients with chronic myeloid leukemia identifies CXCL14 as a new therapeutic option Article Snippet: After spinning down at 800 x g 5 min, the cells were stained with BD Phosflow Alexa Fluor 647 mouse anti-mTOR (pS2448), cat#564242) or purified primary antibodies against OPA3 (ab230205), CYC1 (ab224044), SLC25A26 [EPR11581] (ab175209) and SERCA2 ATPase [EPR9392] (ab150435) in BD Perm/Wash buffer (BD 554723) overnight at 4°C. Article Title: A tumor microenvironment model of chronic lymphocytic leukemia enables drug sensitivity testing to guide precision medicine. Article Snippet: Antibodies against Bcl-2 (pS70) (N46-467), Btk (pY223)/Itk (pY180) (N35-86), Btk (pY551) & p-Itk (pY511) (24a/BTK (Y551), IgG1 Kappa (MOPC-21), MEK1 (pS298) (J114-64), MEK1 (pS218)/MEK2 (pS222) (024-836), mTOR (pS2448) (O21-404), NF-κB p65 (pS529) (K10895.12.50), p53 (pS37) (J159-641.79), PLCγ2 (pY759) (K86-689.37), Rb (pS807/S811) (J112-906), STAT1 (pS272) (K51-856), STAT1 (pY701) (14/P), STAT3 (pS727) (49/p-Stat3), STAT3 (pY705) (4/p-STAT3), STAT5 (pY694) (47), STAT6 (pY614) (18/p-Stat6), TBK1 (pS172) (J133-587), and Article Title: IL-21, Inflammatory Cytokines and Hyperpolarized CD8 + T Cells Are Central Players in Lupus Immune Pathology. Article Snippet: After washing with staining media, cells were stained with p-mTOR (Ser2448) Antibody for 30 min at room temperature and then acquired on a BD LSR Fortessa [30,32]. Article Title: Amniotic MSC affect CD8 naive polarization toward SLEC/MPEC subsets by down-modulating IL-12Rβ1 and IL-2Rα signaling pathways Article Snippet: Purification:Article Title: IL-21, inflammatory cytokines and hyper polarized CD8 + T cells are central players in Lupus immune pathology Article Snippet: Then they were permeabilised with ice cold methanol with vigorous vortexing and incubated at 4° C for 15mins.After washing with staining media cells were stained with p-mTOR (Ser2448) Antibody for 30 mins at room temperature and then acquired on BD LSR Fortessa[ , ]. Article Title: Functional characterization of the PI3K/AKT/MTOR signaling pathway for targeted therapy in B-precursor acute lymphoblastic leukemia. Article Snippet: Alexa 647 p-MTOR (Ser2448) (clone O21-404) and PE p-AKT (Ser473) (clone M89-61) for Article Title: Amniotic MSC affect CD8 naive polarization toward SLEC/MPEC subsets by down-modulating IL-12Rβ1 and IL-2Rα signaling pathways Article Snippet: Finally, cells were stained for 1h at RT with BD PhosflowTM (BD Bioscience) fluorescently conjugated antibodies against: Stat4 (pY693) Alexa fluor® 488 (clone 38/p-Stat4), Stat5 (pY694) PE-Cy7 (clone 47/Stat5(pY694)), Anti-Akt (pS473) PE-CF594 (clone M89-61), Anti-mTOR (pS2448) Alexa fluor® 647 (clone 021-404) all purchased from BD Biosciences. Article Title: IL-21, Inflammatory Cytokines and Hyperpolarized CD8 + T Cells Are Central Players in Lupus Immune Pathology Article Snippet: After washing with staining media, cells were stained with p-mTOR (Ser2448) Antibody for 30 min at room temperature and then acquired on a BD LSR Fortessa [ , ]. Article Title: Characterization of the bone marrow niche in patients with chronic myeloid leukemia identifies CXCL14 as a new therapeutic option Article Snippet: After spinning down at 800 x g 5 min, the cells were stained with BD Phosflow Alexa Fluor 647 mouse anti-mTOR (pS2448), cat#564242) or purified primary antibodies against OPA3 (ab230205), CYC1 (ab224044), SLC25A26 [EPR11581] (ab175209) and SERCA2 ATPase [EPR9392] (ab150435) in BD Perm/Wash buffer (BD 554723) overnight at 4°C. Article Title: A tumor microenvironment model of chronic lymphocytic leukemia enables drug sensitivity testing to guide precision medicine. Article Snippet: Antibodies against Bcl-2 (pS70) (N46-467), Btk (pY223)/Itk (pY180) (N35-86), Btk (pY551) & p-Itk (pY511) (24a/BTK (Y551), IgG1 Kappa (MOPC-21), MEK1 (pS298) (J114-64), MEK1 (pS218)/MEK2 (pS222) (024-836), mTOR (pS2448) (O21-404), NF-κB p65 (pS529) (K10895.12.50), p53 (pS37) (J159-641.79), PLCγ2 (pY759) (K86-689.37), Rb (pS807/S811) (J112-906), STAT1 (pS272) (K51-856), STAT1 (pY701) (14/P), STAT3 (pS727) (49/p-Stat3), STAT3 (pY705) (4/p-STAT3), STAT5 (pY694) (47), STAT6 (pY614) (18/p-Stat6), TBK1 (pS172) (J133-587), and Article Title: IL-21, Inflammatory Cytokines and Hyperpolarized CD8 + T Cells Are Central Players in Lupus Immune Pathology. Article Snippet: After washing with staining media, cells were stained with p-mTOR (Ser2448) Antibody for 30 min at room temperature and then acquired on a BD LSR Fortessa [30,32]. Article Title: Amniotic MSC affect CD8 naive polarization toward SLEC/MPEC subsets by down-modulating IL-12Rβ1 and IL-2Rα signaling pathways Article Snippet: Expressing:Article Title: IL-21, inflammatory cytokines and hyper polarized CD8 + T cells are central players in Lupus immune pathology Article Snippet: Then they were permeabilised with ice cold methanol with vigorous vortexing and incubated at 4° C for 15mins.After washing with staining media cells were stained with p-mTOR (Ser2448) Antibody for 30 mins at room temperature and then acquired on BD LSR Fortessa[ , ]. Article Title: Functional characterization of the PI3K/AKT/MTOR signaling pathway for targeted therapy in B-precursor acute lymphoblastic leukemia. Article Snippet: Alexa 647 p-MTOR (Ser2448) (clone O21-404) and PE p-AKT (Ser473) (clone M89-61) for Article Title: Amniotic MSC affect CD8 naive polarization toward SLEC/MPEC subsets by down-modulating IL-12Rβ1 and IL-2Rα signaling pathways Article Snippet: Finally, cells were stained for 1h at RT with BD PhosflowTM (BD Bioscience) fluorescently conjugated antibodies against: Stat4 (pY693) Alexa fluor® 488 (clone 38/p-Stat4), Stat5 (pY694) PE-Cy7 (clone 47/Stat5(pY694)), Anti-Akt (pS473) PE-CF594 (clone M89-61), Anti-mTOR (pS2448) Alexa fluor® 647 (clone 021-404) all purchased from BD Biosciences. Article Title: IL-21, Inflammatory Cytokines and Hyperpolarized CD8 + T Cells Are Central Players in Lupus Immune Pathology Article Snippet: After washing with staining media, cells were stained with p-mTOR (Ser2448) Antibody for 30 min at room temperature and then acquired on a BD LSR Fortessa [ , ]. Article Title: Characterization of the bone marrow niche in patients with chronic myeloid leukemia identifies CXCL14 as a new therapeutic option Article Snippet: After spinning down at 800 x g 5 min, the cells were stained with BD Phosflow Alexa Fluor 647 mouse anti-mTOR (pS2448), cat#564242) or purified primary antibodies against OPA3 (ab230205), CYC1 (ab224044), SLC25A26 [EPR11581] (ab175209) and SERCA2 ATPase [EPR9392] (ab150435) in BD Perm/Wash buffer (BD 554723) overnight at 4°C. Article Title: A tumor microenvironment model of chronic lymphocytic leukemia enables drug sensitivity testing to guide precision medicine. Article Snippet: Antibodies against Bcl-2 (pS70) (N46-467), Btk (pY223)/Itk (pY180) (N35-86), Btk (pY551) & p-Itk (pY511) (24a/BTK (Y551), IgG1 Kappa (MOPC-21), MEK1 (pS298) (J114-64), MEK1 (pS218)/MEK2 (pS222) (024-836), mTOR (pS2448) (O21-404), NF-κB p65 (pS529) (K10895.12.50), p53 (pS37) (J159-641.79), PLCγ2 (pY759) (K86-689.37), Rb (pS807/S811) (J112-906), STAT1 (pS272) (K51-856), STAT1 (pY701) (14/P), STAT3 (pS727) (49/p-Stat3), STAT3 (pY705) (4/p-STAT3), STAT5 (pY694) (47), STAT6 (pY614) (18/p-Stat6), TBK1 (pS172) (J133-587), and Article Title: IL-21, Inflammatory Cytokines and Hyperpolarized CD8 + T Cells Are Central Players in Lupus Immune Pathology. Article Snippet: After washing with staining media, cells were stained with p-mTOR (Ser2448) Antibody for 30 min at room temperature and then acquired on a BD LSR Fortessa [30,32]. Article Title: Amniotic MSC affect CD8 naive polarization toward SLEC/MPEC subsets by down-modulating IL-12Rβ1 and IL-2Rα signaling pathways Article Snippet: Isolation:Article Title: IL-21, inflammatory cytokines and hyper polarized CD8 + T cells are central players in Lupus immune pathology Article Snippet: Then they were permeabilised with ice cold methanol with vigorous vortexing and incubated at 4° C for 15mins.After washing with staining media cells were stained with p-mTOR (Ser2448) Antibody for 30 mins at room temperature and then acquired on BD LSR Fortessa[ , ]. Article Title: Functional characterization of the PI3K/AKT/MTOR signaling pathway for targeted therapy in B-precursor acute lymphoblastic leukemia. Article Snippet: Alexa 647 p-MTOR (Ser2448) (clone O21-404) and PE p-AKT (Ser473) (clone M89-61) for Article Title: Amniotic MSC affect CD8 naive polarization toward SLEC/MPEC subsets by down-modulating IL-12Rβ1 and IL-2Rα signaling pathways Article Snippet: Finally, cells were stained for 1h at RT with BD PhosflowTM (BD Bioscience) fluorescently conjugated antibodies against: Stat4 (pY693) Alexa fluor® 488 (clone 38/p-Stat4), Stat5 (pY694) PE-Cy7 (clone 47/Stat5(pY694)), Anti-Akt (pS473) PE-CF594 (clone M89-61), Anti-mTOR (pS2448) Alexa fluor® 647 (clone 021-404) all purchased from BD Biosciences. Article Title: IL-21, Inflammatory Cytokines and Hyperpolarized CD8 + T Cells Are Central Players in Lupus Immune Pathology Article Snippet: After washing with staining media, cells were stained with p-mTOR (Ser2448) Antibody for 30 min at room temperature and then acquired on a BD LSR Fortessa [ , ]. Article Title: Characterization of the bone marrow niche in patients with chronic myeloid leukemia identifies CXCL14 as a new therapeutic option Article Snippet: After spinning down at 800 x g 5 min, the cells were stained with BD Phosflow Alexa Fluor 647 mouse anti-mTOR (pS2448), cat#564242) or purified primary antibodies against OPA3 (ab230205), CYC1 (ab224044), SLC25A26 [EPR11581] (ab175209) and SERCA2 ATPase [EPR9392] (ab150435) in BD Perm/Wash buffer (BD 554723) overnight at 4°C. Article Title: A tumor microenvironment model of chronic lymphocytic leukemia enables drug sensitivity testing to guide precision medicine. Article Snippet: Antibodies against Bcl-2 (pS70) (N46-467), Btk (pY223)/Itk (pY180) (N35-86), Btk (pY551) & p-Itk (pY511) (24a/BTK (Y551), IgG1 Kappa (MOPC-21), MEK1 (pS298) (J114-64), MEK1 (pS218)/MEK2 (pS222) (024-836), mTOR (pS2448) (O21-404), NF-κB p65 (pS529) (K10895.12.50), p53 (pS37) (J159-641.79), PLCγ2 (pY759) (K86-689.37), Rb (pS807/S811) (J112-906), STAT1 (pS272) (K51-856), STAT1 (pY701) (14/P), STAT3 (pS727) (49/p-Stat3), STAT3 (pY705) (4/p-STAT3), STAT5 (pY694) (47), STAT6 (pY614) (18/p-Stat6), TBK1 (pS172) (J133-587), and Article Title: IL-21, Inflammatory Cytokines and Hyperpolarized CD8 + T Cells Are Central Players in Lupus Immune Pathology. Article Snippet: After washing with staining media, cells were stained with p-mTOR (Ser2448) Antibody for 30 min at room temperature and then acquired on a BD LSR Fortessa [30,32]. Article Title: Amniotic MSC affect CD8 naive polarization toward SLEC/MPEC subsets by down-modulating IL-12Rβ1 and IL-2Rα signaling pathways Article Snippet: Activation Assay:Article Title: IL-21, inflammatory cytokines and hyper polarized CD8 + T cells are central players in Lupus immune pathology Article Snippet: Then they were permeabilised with ice cold methanol with vigorous vortexing and incubated at 4° C for 15mins.After washing with staining media cells were stained with p-mTOR (Ser2448) Antibody for 30 mins at room temperature and then acquired on BD LSR Fortessa[ , ]. Article Title: Functional characterization of the PI3K/AKT/MTOR signaling pathway for targeted therapy in B-precursor acute lymphoblastic leukemia. Article Snippet: Alexa 647 p-MTOR (Ser2448) (clone O21-404) and PE p-AKT (Ser473) (clone M89-61) for Article Title: Amniotic MSC affect CD8 naive polarization toward SLEC/MPEC subsets by down-modulating IL-12Rβ1 and IL-2Rα signaling pathways Article Snippet: Finally, cells were stained for 1h at RT with BD PhosflowTM (BD Bioscience) fluorescently conjugated antibodies against: Stat4 (pY693) Alexa fluor® 488 (clone 38/p-Stat4), Stat5 (pY694) PE-Cy7 (clone 47/Stat5(pY694)), Anti-Akt (pS473) PE-CF594 (clone M89-61), Anti-mTOR (pS2448) Alexa fluor® 647 (clone 021-404) all purchased from BD Biosciences. Article Title: IL-21, Inflammatory Cytokines and Hyperpolarized CD8 + T Cells Are Central Players in Lupus Immune Pathology Article Snippet: After washing with staining media, cells were stained with p-mTOR (Ser2448) Antibody for 30 min at room temperature and then acquired on a BD LSR Fortessa [ , ]. Article Title: Characterization of the bone marrow niche in patients with chronic myeloid leukemia identifies CXCL14 as a new therapeutic option Article Snippet: After spinning down at 800 x g 5 min, the cells were stained with BD Phosflow Alexa Fluor 647 mouse anti-mTOR (pS2448), cat#564242) or purified primary antibodies against OPA3 (ab230205), CYC1 (ab224044), SLC25A26 [EPR11581] (ab175209) and SERCA2 ATPase [EPR9392] (ab150435) in BD Perm/Wash buffer (BD 554723) overnight at 4°C. Article Title: A tumor microenvironment model of chronic lymphocytic leukemia enables drug sensitivity testing to guide precision medicine. Article Snippet: Antibodies against Bcl-2 (pS70) (N46-467), Btk (pY223)/Itk (pY180) (N35-86), Btk (pY551) & p-Itk (pY511) (24a/BTK (Y551), IgG1 Kappa (MOPC-21), MEK1 (pS298) (J114-64), MEK1 (pS218)/MEK2 (pS222) (024-836), mTOR (pS2448) (O21-404), NF-κB p65 (pS529) (K10895.12.50), p53 (pS37) (J159-641.79), PLCγ2 (pY759) (K86-689.37), Rb (pS807/S811) (J112-906), STAT1 (pS272) (K51-856), STAT1 (pY701) (14/P), STAT3 (pS727) (49/p-Stat3), STAT3 (pY705) (4/p-STAT3), STAT5 (pY694) (47), STAT6 (pY614) (18/p-Stat6), TBK1 (pS172) (J133-587), and Article Title: IL-21, Inflammatory Cytokines and Hyperpolarized CD8 + T Cells Are Central Players in Lupus Immune Pathology. Article Snippet: After washing with staining media, cells were stained with p-mTOR (Ser2448) Antibody for 30 min at room temperature and then acquired on a BD LSR Fortessa [30,32]. Article Title: Amniotic MSC affect CD8 naive polarization toward SLEC/MPEC subsets by down-modulating IL-12Rβ1 and IL-2Rα signaling pathways Article Snippet: Flow Cytometry:Article Title: IL-21, inflammatory cytokines and hyper polarized CD8 + T cells are central players in Lupus immune pathology Article Snippet: Then they were permeabilised with ice cold methanol with vigorous vortexing and incubated at 4° C for 15mins.After washing with staining media cells were stained with p-mTOR (Ser2448) Antibody for 30 mins at room temperature and then acquired on BD LSR Fortessa[ , ]. Article Title: Functional characterization of the PI3K/AKT/MTOR signaling pathway for targeted therapy in B-precursor acute lymphoblastic leukemia. Article Snippet: Alexa 647 p-MTOR (Ser2448) (clone O21-404) and PE p-AKT (Ser473) (clone M89-61) for Article Title: Amniotic MSC affect CD8 naive polarization toward SLEC/MPEC subsets by down-modulating IL-12Rβ1 and IL-2Rα signaling pathways Article Snippet: Finally, cells were stained for 1h at RT with BD PhosflowTM (BD Bioscience) fluorescently conjugated antibodies against: Stat4 (pY693) Alexa fluor® 488 (clone 38/p-Stat4), Stat5 (pY694) PE-Cy7 (clone 47/Stat5(pY694)), Anti-Akt (pS473) PE-CF594 (clone M89-61), Anti-mTOR (pS2448) Alexa fluor® 647 (clone 021-404) all purchased from BD Biosciences. Article Title: IL-21, Inflammatory Cytokines and Hyperpolarized CD8 + T Cells Are Central Players in Lupus Immune Pathology Article Snippet: After washing with staining media, cells were stained with p-mTOR (Ser2448) Antibody for 30 min at room temperature and then acquired on a BD LSR Fortessa [ , ]. Article Title: Characterization of the bone marrow niche in patients with chronic myeloid leukemia identifies CXCL14 as a new therapeutic option Article Snippet: After spinning down at 800 x g 5 min, the cells were stained with BD Phosflow Alexa Fluor 647 mouse anti-mTOR (pS2448), cat#564242) or purified primary antibodies against OPA3 (ab230205), CYC1 (ab224044), SLC25A26 [EPR11581] (ab175209) and SERCA2 ATPase [EPR9392] (ab150435) in BD Perm/Wash buffer (BD 554723) overnight at 4°C. Article Title: A tumor microenvironment model of chronic lymphocytic leukemia enables drug sensitivity testing to guide precision medicine. Article Snippet: Antibodies against Bcl-2 (pS70) (N46-467), Btk (pY223)/Itk (pY180) (N35-86), Btk (pY551) & p-Itk (pY511) (24a/BTK (Y551), IgG1 Kappa (MOPC-21), MEK1 (pS298) (J114-64), MEK1 (pS218)/MEK2 (pS222) (024-836), mTOR (pS2448) (O21-404), NF-κB p65 (pS529) (K10895.12.50), p53 (pS37) (J159-641.79), PLCγ2 (pY759) (K86-689.37), Rb (pS807/S811) (J112-906), STAT1 (pS272) (K51-856), STAT1 (pY701) (14/P), STAT3 (pS727) (49/p-Stat3), STAT3 (pY705) (4/p-STAT3), STAT5 (pY694) (47), STAT6 (pY614) (18/p-Stat6), TBK1 (pS172) (J133-587), and Article Title: IL-21, Inflammatory Cytokines and Hyperpolarized CD8 + T Cells Are Central Players in Lupus Immune Pathology. Article Snippet: After washing with staining media, cells were stained with p-mTOR (Ser2448) Antibody for 30 min at room temperature and then acquired on a BD LSR Fortessa [30,32]. Article Title: Amniotic MSC affect CD8 naive polarization toward SLEC/MPEC subsets by down-modulating IL-12Rβ1 and IL-2Rα signaling pathways Article Snippet: Quantitative RT-PCR:Article Title: IL-21, inflammatory cytokines and hyper polarized CD8 + T cells are central players in Lupus immune pathology Article Snippet: Then they were permeabilised with ice cold methanol with vigorous vortexing and incubated at 4° C for 15mins.After washing with staining media cells were stained with p-mTOR (Ser2448) Antibody for 30 mins at room temperature and then acquired on BD LSR Fortessa[ , ]. Article Title: Functional characterization of the PI3K/AKT/MTOR signaling pathway for targeted therapy in B-precursor acute lymphoblastic leukemia. Article Snippet: Alexa 647 p-MTOR (Ser2448) (clone O21-404) and PE p-AKT (Ser473) (clone M89-61) for Article Title: Amniotic MSC affect CD8 naive polarization toward SLEC/MPEC subsets by down-modulating IL-12Rβ1 and IL-2Rα signaling pathways Article Snippet: Finally, cells were stained for 1h at RT with BD PhosflowTM (BD Bioscience) fluorescently conjugated antibodies against: Stat4 (pY693) Alexa fluor® 488 (clone 38/p-Stat4), Stat5 (pY694) PE-Cy7 (clone 47/Stat5(pY694)), Anti-Akt (pS473) PE-CF594 (clone M89-61), Anti-mTOR (pS2448) Alexa fluor® 647 (clone 021-404) all purchased from BD Biosciences. Article Title: IL-21, Inflammatory Cytokines and Hyperpolarized CD8 + T Cells Are Central Players in Lupus Immune Pathology Article Snippet: After washing with staining media, cells were stained with p-mTOR (Ser2448) Antibody for 30 min at room temperature and then acquired on a BD LSR Fortessa [ , ]. Article Title: Characterization of the bone marrow niche in patients with chronic myeloid leukemia identifies CXCL14 as a new therapeutic option Article Snippet: After spinning down at 800 x g 5 min, the cells were stained with BD Phosflow Alexa Fluor 647 mouse anti-mTOR (pS2448), cat#564242) or purified primary antibodies against OPA3 (ab230205), CYC1 (ab224044), SLC25A26 [EPR11581] (ab175209) and SERCA2 ATPase [EPR9392] (ab150435) in BD Perm/Wash buffer (BD 554723) overnight at 4°C. Article Title: A tumor microenvironment model of chronic lymphocytic leukemia enables drug sensitivity testing to guide precision medicine. Article Snippet: Antibodies against Bcl-2 (pS70) (N46-467), Btk (pY223)/Itk (pY180) (N35-86), Btk (pY551) & p-Itk (pY511) (24a/BTK (Y551), IgG1 Kappa (MOPC-21), MEK1 (pS298) (J114-64), MEK1 (pS218)/MEK2 (pS222) (024-836), mTOR (pS2448) (O21-404), NF-κB p65 (pS529) (K10895.12.50), p53 (pS37) (J159-641.79), PLCγ2 (pY759) (K86-689.37), Rb (pS807/S811) (J112-906), STAT1 (pS272) (K51-856), STAT1 (pY701) (14/P), STAT3 (pS727) (49/p-Stat3), STAT3 (pY705) (4/p-STAT3), STAT5 (pY694) (47), STAT6 (pY614) (18/p-Stat6), TBK1 (pS172) (J133-587), and Article Title: IL-21, Inflammatory Cytokines and Hyperpolarized CD8 + T Cells Are Central Players in Lupus Immune Pathology. Article Snippet: After washing with staining media, cells were stained with p-mTOR (Ser2448) Antibody for 30 min at room temperature and then acquired on a BD LSR Fortessa [30,32]. Article Title: Amniotic MSC affect CD8 naive polarization toward SLEC/MPEC subsets by down-modulating IL-12Rβ1 and IL-2Rα signaling pathways Article Snippet: Synthesized:Article Title: IL-21, inflammatory cytokines and hyper polarized CD8 + T cells are central players in Lupus immune pathology Article Snippet: Then they were permeabilised with ice cold methanol with vigorous vortexing and incubated at 4° C for 15mins.After washing with staining media cells were stained with p-mTOR (Ser2448) Antibody for 30 mins at room temperature and then acquired on BD LSR Fortessa[ , ]. Article Title: Functional characterization of the PI3K/AKT/MTOR signaling pathway for targeted therapy in B-precursor acute lymphoblastic leukemia. Article Snippet: Alexa 647 p-MTOR (Ser2448) (clone O21-404) and PE p-AKT (Ser473) (clone M89-61) for Article Title: Amniotic MSC affect CD8 naive polarization toward SLEC/MPEC subsets by down-modulating IL-12Rβ1 and IL-2Rα signaling pathways Article Snippet: Finally, cells were stained for 1h at RT with BD PhosflowTM (BD Bioscience) fluorescently conjugated antibodies against: Stat4 (pY693) Alexa fluor® 488 (clone 38/p-Stat4), Stat5 (pY694) PE-Cy7 (clone 47/Stat5(pY694)), Anti-Akt (pS473) PE-CF594 (clone M89-61), Anti-mTOR (pS2448) Alexa fluor® 647 (clone 021-404) all purchased from BD Biosciences. Article Title: IL-21, Inflammatory Cytokines and Hyperpolarized CD8 + T Cells Are Central Players in Lupus Immune Pathology Article Snippet: After washing with staining media, cells were stained with p-mTOR (Ser2448) Antibody for 30 min at room temperature and then acquired on a BD LSR Fortessa [ , ]. Article Title: Characterization of the bone marrow niche in patients with chronic myeloid leukemia identifies CXCL14 as a new therapeutic option Article Snippet: After spinning down at 800 x g 5 min, the cells were stained with BD Phosflow Alexa Fluor 647 mouse anti-mTOR (pS2448), cat#564242) or purified primary antibodies against OPA3 (ab230205), CYC1 (ab224044), SLC25A26 [EPR11581] (ab175209) and SERCA2 ATPase [EPR9392] (ab150435) in BD Perm/Wash buffer (BD 554723) overnight at 4°C. Article Title: A tumor microenvironment model of chronic lymphocytic leukemia enables drug sensitivity testing to guide precision medicine. Article Snippet: Antibodies against Bcl-2 (pS70) (N46-467), Btk (pY223)/Itk (pY180) (N35-86), Btk (pY551) & p-Itk (pY511) (24a/BTK (Y551), IgG1 Kappa (MOPC-21), MEK1 (pS298) (J114-64), MEK1 (pS218)/MEK2 (pS222) (024-836), mTOR (pS2448) (O21-404), NF-κB p65 (pS529) (K10895.12.50), p53 (pS37) (J159-641.79), PLCγ2 (pY759) (K86-689.37), Rb (pS807/S811) (J112-906), STAT1 (pS272) (K51-856), STAT1 (pY701) (14/P), STAT3 (pS727) (49/p-Stat3), STAT3 (pY705) (4/p-STAT3), STAT5 (pY694) (47), STAT6 (pY614) (18/p-Stat6), TBK1 (pS172) (J133-587), and Article Title: IL-21, Inflammatory Cytokines and Hyperpolarized CD8 + T Cells Are Central Players in Lupus Immune Pathology. Article Snippet: After washing with staining media, cells were stained with p-mTOR (Ser2448) Antibody for 30 min at room temperature and then acquired on a BD LSR Fortessa [30,32]. Article Title: Amniotic MSC affect CD8 naive polarization toward SLEC/MPEC subsets by down-modulating IL-12Rβ1 and IL-2Rα signaling pathways Article Snippet: Reverse Transcription:Article Title: IL-21, inflammatory cytokines and hyper polarized CD8 + T cells are central players in Lupus immune pathology Article Snippet: Then they were permeabilised with ice cold methanol with vigorous vortexing and incubated at 4° C for 15mins.After washing with staining media cells were stained with p-mTOR (Ser2448) Antibody for 30 mins at room temperature and then acquired on BD LSR Fortessa[ , ]. Article Title: Functional characterization of the PI3K/AKT/MTOR signaling pathway for targeted therapy in B-precursor acute lymphoblastic leukemia. Article Snippet: Alexa 647 p-MTOR (Ser2448) (clone O21-404) and PE p-AKT (Ser473) (clone M89-61) for Article Title: Amniotic MSC affect CD8 naive polarization toward SLEC/MPEC subsets by down-modulating IL-12Rβ1 and IL-2Rα signaling pathways Article Snippet: Finally, cells were stained for 1h at RT with BD PhosflowTM (BD Bioscience) fluorescently conjugated antibodies against: Stat4 (pY693) Alexa fluor® 488 (clone 38/p-Stat4), Stat5 (pY694) PE-Cy7 (clone 47/Stat5(pY694)), Anti-Akt (pS473) PE-CF594 (clone M89-61), Anti-mTOR (pS2448) Alexa fluor® 647 (clone 021-404) all purchased from BD Biosciences. Article Title: IL-21, Inflammatory Cytokines and Hyperpolarized CD8 + T Cells Are Central Players in Lupus Immune Pathology Article Snippet: After washing with staining media, cells were stained with p-mTOR (Ser2448) Antibody for 30 min at room temperature and then acquired on a BD LSR Fortessa [ , ]. Article Title: Characterization of the bone marrow niche in patients with chronic myeloid leukemia identifies CXCL14 as a new therapeutic option Article Snippet: After spinning down at 800 x g 5 min, the cells were stained with BD Phosflow Alexa Fluor 647 mouse anti-mTOR (pS2448), cat#564242) or purified primary antibodies against OPA3 (ab230205), CYC1 (ab224044), SLC25A26 [EPR11581] (ab175209) and SERCA2 ATPase [EPR9392] (ab150435) in BD Perm/Wash buffer (BD 554723) overnight at 4°C. Article Title: A tumor microenvironment model of chronic lymphocytic leukemia enables drug sensitivity testing to guide precision medicine. Article Snippet: Antibodies against Bcl-2 (pS70) (N46-467), Btk (pY223)/Itk (pY180) (N35-86), Btk (pY551) & p-Itk (pY511) (24a/BTK (Y551), IgG1 Kappa (MOPC-21), MEK1 (pS298) (J114-64), MEK1 (pS218)/MEK2 (pS222) (024-836), mTOR (pS2448) (O21-404), NF-κB p65 (pS529) (K10895.12.50), p53 (pS37) (J159-641.79), PLCγ2 (pY759) (K86-689.37), Rb (pS807/S811) (J112-906), STAT1 (pS272) (K51-856), STAT1 (pY701) (14/P), STAT3 (pS727) (49/p-Stat3), STAT3 (pY705) (4/p-STAT3), STAT5 (pY694) (47), STAT6 (pY614) (18/p-Stat6), TBK1 (pS172) (J133-587), and Article Title: IL-21, Inflammatory Cytokines and Hyperpolarized CD8 + T Cells Are Central Players in Lupus Immune Pathology. Article Snippet: After washing with staining media, cells were stained with p-mTOR (Ser2448) Antibody for 30 min at room temperature and then acquired on a BD LSR Fortessa [30,32]. Article Title: Amniotic MSC affect CD8 naive polarization toward SLEC/MPEC subsets by down-modulating IL-12Rβ1 and IL-2Rα signaling pathways Article Snippet: Incubation:Article Title: IL-21, inflammatory cytokines and hyper polarized CD8 + T cells are central players in Lupus immune pathology Article Snippet: Then they were permeabilised with ice cold methanol with vigorous vortexing and incubated at 4° C for 15mins.After washing with staining media cells were stained with p-mTOR (Ser2448) Antibody for 30 mins at room temperature and then acquired on BD LSR Fortessa[ , ]. Article Title: Functional characterization of the PI3K/AKT/MTOR signaling pathway for targeted therapy in B-precursor acute lymphoblastic leukemia. Article Snippet: Alexa 647 p-MTOR (Ser2448) (clone O21-404) and PE p-AKT (Ser473) (clone M89-61) for Article Title: Amniotic MSC affect CD8 naive polarization toward SLEC/MPEC subsets by down-modulating IL-12Rβ1 and IL-2Rα signaling pathways Article Snippet: Finally, cells were stained for 1h at RT with BD PhosflowTM (BD Bioscience) fluorescently conjugated antibodies against: Stat4 (pY693) Alexa fluor® 488 (clone 38/p-Stat4), Stat5 (pY694) PE-Cy7 (clone 47/Stat5(pY694)), Anti-Akt (pS473) PE-CF594 (clone M89-61), Anti-mTOR (pS2448) Alexa fluor® 647 (clone 021-404) all purchased from BD Biosciences. Article Title: IL-21, Inflammatory Cytokines and Hyperpolarized CD8 + T Cells Are Central Players in Lupus Immune Pathology Article Snippet: After washing with staining media, cells were stained with p-mTOR (Ser2448) Antibody for 30 min at room temperature and then acquired on a BD LSR Fortessa [ , ]. Article Title: Characterization of the bone marrow niche in patients with chronic myeloid leukemia identifies CXCL14 as a new therapeutic option Article Snippet: After spinning down at 800 x g 5 min, the cells were stained with BD Phosflow Alexa Fluor 647 mouse anti-mTOR (pS2448), cat#564242) or purified primary antibodies against OPA3 (ab230205), CYC1 (ab224044), SLC25A26 [EPR11581] (ab175209) and SERCA2 ATPase [EPR9392] (ab150435) in BD Perm/Wash buffer (BD 554723) overnight at 4°C. Article Title: A tumor microenvironment model of chronic lymphocytic leukemia enables drug sensitivity testing to guide precision medicine. Article Snippet: Antibodies against Bcl-2 (pS70) (N46-467), Btk (pY223)/Itk (pY180) (N35-86), Btk (pY551) & p-Itk (pY511) (24a/BTK (Y551), IgG1 Kappa (MOPC-21), MEK1 (pS298) (J114-64), MEK1 (pS218)/MEK2 (pS222) (024-836), mTOR (pS2448) (O21-404), NF-κB p65 (pS529) (K10895.12.50), p53 (pS37) (J159-641.79), PLCγ2 (pY759) (K86-689.37), Rb (pS807/S811) (J112-906), STAT1 (pS272) (K51-856), STAT1 (pY701) (14/P), STAT3 (pS727) (49/p-Stat3), STAT3 (pY705) (4/p-STAT3), STAT5 (pY694) (47), STAT6 (pY614) (18/p-Stat6), TBK1 (pS172) (J133-587), and Article Title: IL-21, Inflammatory Cytokines and Hyperpolarized CD8 + T Cells Are Central Players in Lupus Immune Pathology. Article Snippet: After washing with staining media, cells were stained with p-mTOR (Ser2448) Antibody for 30 min at room temperature and then acquired on a BD LSR Fortessa [30,32]. Article Title: Amniotic MSC affect CD8 naive polarization toward SLEC/MPEC subsets by down-modulating IL-12Rβ1 and IL-2Rα signaling pathways Article Snippet: |